• Environmental Engineering Research
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• v.23 no.4
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• pp.420-429
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• 2018

Soil stabilization is a soil remediation technique that reduces the mobility of heavy metals in soils. Although it is a well-established technique, it is nonetheless essential to perform a follow-up chemical assessment via a leaching test to evaluate the immobilization of heavy metals in the soil matrix. Unfortunately, a standard chemical assessment is not sufficient for evaluation of the biological functional state of stabilized soils slated for agricultural use. Therefore, it is useful to employ a pyrosequencing-based microbial community analysis for the purpose. In this study, a recently stabilized site in the proximity of an exhausted mine was analyzed for bacterial diversity, richness, and relative abundance as well as the effect of environmental factors. Based on the Shannon and Chao1 indices and rarefaction curves, the results showed that the stabilized layer exhibited lower bacterial diversity than control soils. The prevalence of dominant bacterial populations was examined in a hierarchical manner. Relatively high abundances of Proteobacteria and Methylobacter tundripaludum were observed in the stabilized soil. In particular, there was substantial abundance of the Methylobacter genus, which is known for its association with heavy metal contamination. The study demonstrated the efficacy of (micro)biological assessment for aiding in the understanding and post-management of stabilized soils.

• Journal of the Korean Society of Systems Engineering
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• v.8 no.2
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• pp.37-45
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• 2012

About 20 years have passed since Systems Engineering(SE) was introduced in Korea. KCOSE(Korean Council On Systems Engineering) and KOSSE(The Korean Society of Systems Engineering) were established during that period and Korean SE community has increased quantitatively. But We can not answer "Yes!" for the questions "Did Korean SE community increase qualitatively? and How about SE education or training?" Many participants at SE lecture classes said that they were not able to understand SE concept. I analyzed current SE lecture materials and then proposed new model of SE concept lecture for SE novices. The new model consists of Vee diagram, life cycle model, PMTE paradigm, technical review, SE sample and definition of SE. I expect that the proposed model will contribute to upgrade a quality of domestic SE education.

• Journal of the Korea Society of Computer and Information
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• v.19 no.12
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• pp.239-246
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• 2014

In this paper, we propose a method to decide the additional edges in order to integrate two communitites A,B($${\mid}A{\mid}{\geq_-}{\mid}B{\mid}$$, ${\mid}{\cdot}{\mid}$ is the size of the set). The proposed algorithm uses a fitness function that shows the property of a community and the fitness function is defined by the number of edges which exist in the community and connect two nodes, one is in the community and the other is out of the community. The community has a strong property when the function has a large value. The proposed algorithm is a kind of greedy method and when a node of B is merged to A, the minimum number of additional edges is decided to increase the fitness function value of A. After determining the number of additional edges, we define the community connectivity measures using the node centrality to determine the edges locations. The connections of the new edges are fixed to maximize the connectivity measure of the combined community. The procedure is applied for all nodes in B to integrate A and B. The effectiveness of the proposed algorithm is shown by solving the Zachary Karate Club network.

• Korean Journal of Environmental Biology
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• v.31 no.4
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• pp.362-369
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• 2013

Phytoplankton and zooplankton communities were investigated from 8 weir sites of the Nakdong River system to provide basic information on plankton community after weir construction and to estimate its effects on major water quality parameters. The relationship between plankton community structure and environmental factors was analysed with CCA (Canonical Correspondence Analysis). The results suggested that discharge and total phosphorus and nitrogen concentrations are important factors determining the phytoplankton species composition. For zooplankton community, the difference in discharge between September and October induced different distribution pattern of zooplankton community with more homogeneous distribution with extreme dominance of rotifers during the high discharge season. Chlorophyll a concentration representing phytoplankton biomass has been suggested as the main environmental factor affecting zooplankton community followed by COD and total nitrogen concentration.

• Journal of environmental and Sanitary engineering
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• v.6 no.1
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• pp.27-30
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• 1991

Since the diffusion rate of sewage treatment plant is about 30% of the country. It is not enough to cope with entire sewage treatment of the community. Therefore, the septic tank is introduced which is connected with water flush toilet only. It is estimated that the large portion of water pollution has occured due to untreated sewage such as pollutants of kitchen, laundry and bathtub water, etc., which are not go through the existing septic tank. However, the construction of community plant which is could treat most of household run sewage should have flexibility in type, treatment method, effectiveness and economy which are adoption of the community. Without the treatment of combined household run sewage, the improvement of water pollution will be hardly expected.

• Journal of Korean Society on Water Environment
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• v.31 no.4
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• pp.399-406
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• 2015

A sequencing batch reactor was operated under different pH conditions to see the influence of free ammonia (FA) and free nitrous acid (FNA) to microbial community on ammonium partial nitrification. Long-term influences of FA and FNA were evaluated by polymerase chain reaction-denaturing gradient gel electrophoresis and fluorescence in situ hybridization. Nitrite accumulation was successfully achieved at pH 8.2 and 6.3. The shifts in the microbial community were observed when influent ammonia concentration increased to 1 g $NH_4$-N/L at pH 8.2, and then when pH was dropped to 6.3. Both Nitrosomonas and Nitrosospira were selected during the startup of the reactor, and eventually became dominant members as ammonia-oxidizing bacteria. The results of molecular microbiological analysis strongly suggested that the composition of microbial community was changed according to the method used to control nitrite-oxidizing bacteria.

• The Plant Pathology Journal
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• v.38 no.5
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• pp.425-431
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• 2022

Plant microbiota has influenced plant growth and physiology significantly. Plant and plant-associated microbes have flexible interactions that respond to changes in environmental conditions. These interactions can be adjusted to suit the requirements of the microbial community or the host physiology. In addition, it can be modified to suit microbiota structure or fixed by the host condition. However, no technology is realized yet to control mechanically manipulated plant microbiota structure. Here, we review step-by-step plant-associated microbial partnership from plant growth-promoting rhizobacteria to the microbiota structural modulation. Glutamic acid enriched the population of Streptomyces, a specific taxon in anthosphere microbiota community. Additionally, the population density of the microbes in the rhizosphere was also a positive response to glutamic acid treatment. Although many types of research are conducted on the structural revealing of plant microbiota, these concepts need to be further understood as to how the plant microbiota clusters are controlled or modulated at the community level. This review suggests that the intrinsic level of glutamic acid in planta is associated with the microbiota composition that the external supply of the biostimulant can modulate.

• Journal of Korean Society on Water Environment
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• v.22 no.4
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• pp.590-598
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• 2006

In-situ Air sparging (IAS) is a groundwater remediation technique, in which organic contaminants volatilize into air form the saturated to vadose zone. This study was carried out to evaluate the effect of sludge and soil microbial community structure on air sparging of Total Petroleum Hydrocarbons (TPH) contaminated groundwater soils. In the laboratory, diesel (10,000 mg TPH/kg) contaminated saturated soil. The Air was injected in intermittent (Q=1500 mL/min, 10 minute injection and 10 minute idle) modes. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for experiment with sludge soil samples that were closely related to Agrococcus, Flavobacterium, Thermoanaerobacter, Flexibacter and Shewanella, etc, in the clone library. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil the fate of microorganisms in natural microbial community.

• Proceedings of the Microbiological Society of Korea Conference
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• 2008.05a
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• pp.118-118
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• 2008

The phylogenetic diversity of microbial communities in calcareous mats from Spearfish Creek, a freshwater stream located in the Black Hills of South Dakota, was examined using PCR-based 16S rDNA sequence analysis. In this study, two types of calcareous mats were compared: mature mats formed on the natural substrate of rock surfaces and developing mats on an artificial substrate of glass slides. Among 63 unique isolates from a clone library of 16S rRNA genes from mature mat samples, there were 8 phyla of Bacteria represented. The predominant phylum was Proteobacteria (48%), with the $\beta$ subclass being the largest group. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes from slide samples collected at intervals for four months showed considerable diversity of the microbial community from the earliest stages of community development. Amplicons isolated from DGGE gels and sequenced indicated that community succession has occurred without increasing microbial diversity. However, light microscopic analysis revealed a significant increase in microbial cell density throughout the community development. Scanning electron microscopy of mat samples provides evidence that diatoms are also important members of calcareous mat communities.

• Journal of Microbiology and Biotechnology
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• v.24 no.3
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• pp.313-323
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• 2014

Significant alteration in the microbial community can occur across reclamation areas suffering subsidence from mining. A reclamation site undergoing fertilization practices and an adjacent coal-excavated subsidence site (sites A and B, respectively) were examined to characterize the bacterial diversity using 454 high-throughput 16S rDNA sequencing. The dominant taxonomic groups in both the sites were Proteobacteria, Acidobacteria, Bacteroidetes, Betaproteobacteria, Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, and Firmicutes. However, the bacterial communities' abundance, diversity, and composition differed significantly between the sites. Site A presented higher bacterial diversity and more complex community structures than site B. The majority of sequences related to Proteobacteria, Gemmatimonadetes, Chloroflexi, Nitrospirae, Firmicutes, Betaproteobacteria, Deltaproteobacteria, and Anaerolineae were from site A; whereas those related to Actinobacteria, Planctomycetes, Bacteroidetes, Verrucomicrobia, Gammaproteobacteria, Nitriliruptoria, Alphaproteobacteria, and Phycisphaerae originated from site B. The distribution of some bacterial groups and subgroups in the two sites correlated with soil properties and vegetation due to reclamation practice. Site A exhibited enriched bacterial community, soil organic matter (SOM), and total nitrogen (TN), suggesting the presence of relatively diverse microorganisms. SOM and TN were important factors shaping the underlying microbial communities. Furthermore, the specific plant functional group (legumes) was also an important factor influencing soil microbial community composition. Thus, the effectiveness of 454 pyrosequencing in analyzing soil bacterial diversity was validated and an association between land ecological system restoration, mostly mediated by microbial communities, and an improvement in soil properties in coal-mining reclamation areas was suggested.