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Role of Trehalose Synthesis in Ralstonia syzygii subsp. indonesiensis PW1001 in Inducing Hypersensitive Response on Eggplant (Solanum melongena cv. Senryo-nigou)

  • Laili, Nur;Mukaihara, Takafumi;Matsui, Hidenori;Yamamoto, Mikihiro;Noutoshi, Yoshiteru;Toyoda, Kazuhiro;Ichinose, Yuki
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.566-579
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    • 2021
  • Ralstonia syzygii subsp. indonesiensis (Rsi, former name: Ralstonia solanacearum phylotype IV) PW1001, a causal agent of potato wilt disease, induces hypersensitive response (HR) on its non-host eggplant (Solanum melongena cv. Senryo-nigou). The disaccharide trehalose is involved in abiotic and biotic stress tolerance in many organisms. We found that trehalose is required for eliciting HR on eggplant by plant pathogen Rsi PW1001. In R. solanacearum, it is known that the OtsA/OtsB pathway is the dominant trehalose synthesis pathway, and otsA and otsB encode trehalose-6-phosphate (T6P) synthase and T6P phosphatase, respectively. We generated otsA and otsB mutant strains and found that these mutant strains reduced the bacterial trehalose concentration and HR induction on eggplant leaves compared to wild-type. Trehalose functions intracellularly in Rsi PW1001 because addition of exogenous trehalose did not affect the HR level and ion leakage. Requirement of trehalose in HR induction is not common in R. solanacearum species complex because mutation of otsA in Ralstonia pseudosolanacearum (former name: Ralstonia solanacearum phylotype I) RS1002 did not affect HR on the leaves of its non-host tobacco and wild eggplant Solanum torvum. Further, we also found that each otsA and otsB mutant had reduced ability to grow in a medium containing NaCl and sucrose, indicating that trehalose also has an important role in osmotic stress tolerance.

Automatic Clustering of Same-Name Authors Using Full-text of Articles (논문 원문을 이용한 동명 저자 자동 군집화)

  • Kang, In-Su;Jung, Han-Min;Lee, Seung-Woo;Kim, Pyung;Goo, Hee-Kwan;Lee, Mi-Kyung;Goo, Nam-Ang;Sung, Won-Kyung
    • Proceedings of the Korea Contents Association Conference
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    • 2006.11a
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    • pp.652-656
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    • 2006
  • Bibliographic information retrieval systems require bibliographic data such as authors, organizations, source of publication to be uniquely identified using keys. In particular, when authors are represented simply as their names, users bear the burden of manually discriminating different users of the same name. Previous approaches to resolving the problem of same-name authors rely on bibliographic data such as co-author information, titles of articles, etc. However, these methods cannot handle the case of single author articles, or the case when articles do not have common terms in their titles. To complement the previous methods, this study introduces a classification-based approach using similarity between full-text of articles. Experiments using recent domestic proceedings showed that the proposed method has the potential to supplement the previous meta-data based approaches.

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The Regional Folk Plants in Southern Inland Area of Gyeonggi-do (경기남부 내륙지역의 민속식물자원)

  • Jeong, Hye-Ran;Choi, Kyung;Park, Kwang-Woo
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.523-542
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    • 2012
  • This study was carried out to clarify the regional folk plants in the southern inland area of Gyeonggi-do Province. According to the survey results, derived from 1,229 sheets of 90 residents at 35 places from 9 counties and cities between October 2009 and December 2010, the folk plants in the inland southern area of Gyeonggi-do consisted of a total of 259 taxa; 82 families, 200 genera, 221 species, 4 subspecies, 32 varieties, and 2 forma. The classification of the plants by its usage was; 214 as edible, 119 as medicinal, 34 as ornamental, 18 as timber, 12 as dye, 5 as oil, 3 as spice, 2 as fiber, 2 as aroma, and 22 as others uses. So, the edible and medicinal uses are higher than other uses. The most useful part was the leaf, followed by fruit, stem, root, and flower. The collection season was focused on spring and summer, because the leaf was mainly collected in an edible condition during those seasons. Traditional knowledge of folk plants was mostly passed down orally. The consistency comparison between the standard common name and the local name was the highest in the people in 50s and the lowest in those in 80s.

The Educational Significance of Place Experience through Folklore (설화를 통한 장소 경험의 융합교육적 의의 -청주 지역 전승의 <지네 장터> 설화를 중심으로)

  • Hwang, Yun-jeong;Seo, Myug-hee
    • Journal of Korean Classical Literature and Education
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    • no.34
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    • pp.75-113
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    • 2017
  • The purpose of fusion education is to acquire educational contents efficiently and gain a new worldview. To realize this purpose, I point out that it is urgent to provide common educational content and suggest "place experience" as common content for literature and geography. Local legends present a concrete space and a sharp confrontation with the human world, while shaping the tradition of a place's name. Place experience as common educational content enables a three-dimensional experience of a place that utilizes the characteristics of these local legends. The physical condition, human activity, and implied meaning of a place mediate the student's empirical understanding of folktales. The common area of "place experience" allows us to expect a stereotypical understanding of a learner's place by providing a literary context to learning contents that can flow from the existing geography subject to the simple provision of information. In addition, it facilitates learners' empirical understanding by providing actual and specific objects to learning contents, which can flow abstractly in the existing literature subject. Through this discussion, convergence education demonstrates educational significance by achieving educational efficiency through common educational content and enabling the formation of new thinking.

Different Characteristics of Toxic Substance/poison Exposure Data that Collected from Pre-hospital Telephone Response and Emergency Department (일부 지역의 전화상담을 통해 얻어진 독성물질 노출정보와 응급실 기반 중독 정보 분석)

  • Kim, Su-Jin;Choa, Min-Hong;Park, Jong-Su;Lee, Sung-Woo;Hong, Yun-Sik
    • Journal of The Korean Society of Clinical Toxicology
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    • v.12 no.1
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    • pp.1-7
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    • 2014
  • Purpose: The purpose of this study is to find differences in the demographics of toxic exposed patients and substance between call based poison information data and hospital based poison information data. Methods: Seoul 1339 call-response data were used as call based poison data and toxic related injury surveillance data of the Korean center for disease control and prevention (KCDC) were used as hospital based poison data. Age, sex, the kind of exposed substance, reasons for exposure, and exposure routes were compared between two data sets. We analyzed the presence or not of documentation on the name and amount of exposed substance, symptoms after exposure in call based poison data. Results: Seoul1339 poison data included a total of 2260 information related to toxic exposure and KCDC poison data included 5650 poison cases. There was no difference in sexual distribution. Pediatric exposure and accidental exposure were more common in call based poison data. The most common exposed substances were household products in call based poison data and medicines in hospital based poison data, respectively. Documents regarding amount and time of toxic exposure and symptoms after toxic exposure were not recorded exactly in call based poison data. Conclusion: There were significant differences in age, reasons for toxic exposure, and the kinds of exposed substances. Poison information data from both pre-hospital and hospital must be considered.

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The anti-hyperglycemic property of different ginseng partitions

  • Xie, Jing-Tian;Wang, Chong-Zhi;Kim, Stephen;Yuan, Chun-Su
    • Advances in Traditional Medicine
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    • v.5 no.1
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    • pp.1-15
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    • 2005
  • Ginseng is a popular medicinal plant highly valued throughout the world. Asian ginseng is one of the most common species of ginseng. It has long occupied a significant position in oriental medicine and has been justified its name as the 'king herb'. As a nutritional supplement, ginseng is an extremely common and popular herbal medicine in the United States and Canada in recent decades. The multiple constituents of ginseng possess equally multifaceted pharmacological actions as demonstrated by numerous studies. Ginseng root and its constituents influenced the central nervous system, endocrine, cardiovascular, gastrointestinal system, sexual, renal organ and immune system, etc. One important action is its anti-hyperglycemic effect. Previous studies on ginseng demonstrate that only the root of ginseng has been used in the treatment of diabetes, while the other parts of ginseng plant were always neglected. Recently, we analyzed the constituents of ginseng berry, leaf and discovered that ginseng berry, leaf extracts and its total ginsenosides have the ability to reduce hyperglycemia and body weight and increase the peripheral glucose utilization in obese or diabetic ob/ob or db/db mice. Our data suggest that all parts of ginseng plant, including root, berry, leaf and stem exhibit potent anti-hyperglycemic and anti-obese effects and may provide an opportunity to develop a novel class of anti-diabetic agents.

The Occurrence of Strawberry Black Leaf Spot Caused by Alternaria alternate (Fr.) Keissler in Korea (딸기 검은무늬병(Alternaria alternata (Fr.) Keissler)의 발생에 관하여)

  • Cho J.T.;Moon B.J.
    • Korean journal of applied entomology
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    • v.19 no.4 s.45
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    • pp.221-227
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    • 1980
  • A new disease of strawberry caused by Alternaria was found in Gim Hae, Gyougnam province, in July 1979. The symptoms of the disease occurred mainly on leaves as showing dark brown spots with circular to irregular, $5\~6mm$ in diameter. The pathogen was identified as Alternaria alternate (Fr.) Keissler which has not been described in Korea as a pathogen of strawberry disease. The common name of the disease was given as Gum un mum byung of strawberry. The conidia shape and size of this fungus varied considerably with culture media. The measurements of conidia and beak on corn meal agar were $20.1\times9.3{\mu}\;and\;4.0\mu$, respectively, which were shorter than those on strawberry leaf lesions in the field. Among 49 strawberry varieties tested, Morioca 16 and Robinson were highly susceptible to the disease by artificial inoculation and Catskill, Cyclone, Northwest, Merton princess, Juspa and Daehak I were moderately susceptible whereas 31 varieties were resistant. This fungus could also infect the leaf of Common garden sunflower, Double sunflower and Mexican sunflower among the 14 plants species tested.

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Study on Medical Thought of "Zhouyi Cantongxie(周易參同契)" ("주역참동계"에 나타난 의학사상 연구)

  • Im, Myung-Jin;Kim, Byung-Soo;Kang, Jung-Soo
    • Journal of Korean Medical classics
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    • v.24 no.5
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    • pp.1-10
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    • 2011
  • According to "Dongeuibogam(東醫寶鑑)", "the Shinnong Herb Bible(神農本草經)", "Chamdonggo(參同攷)", come to the conclusion about medical thought of "the Zhouyi Cantongxie(周易參同契)". Alchemy is a common field both on the eastern and western civilization, but a process of development is different. Ancient chinese alchemist made a study on external alchemy, but harmful consequences of external alchemy was consecutive, many scholars had turned their interest on internal alchemy which is related in human meridian biology and spirit. In the western alchemy has developed to make a study on analytic psychology and science of religion. Most importantly, the eastern and western alchemy have something in common what at the point of the perfection of self is similar, but the name is different, for example in the eastern alchemy we can be immortals and take a spiritual enlightenment with the elixir of life, in the western we can take a cure of souls as a christian. As for internal alchemy, "Cantongxie(參同契)" gives influence to the theories such as the theory of danjeon(lower part of the abdomen), evolution, aging, and life nurturing. Especially, the theory of danjeon shows such merit for studying since it is similar to triple energizers(三焦) and the theory of qi street(氣街) of oriental medicine. The study on "Cantongxie(參同契)" was achieved in Korea too, especially Chosun dynasty. "Candongkao (參同攷)" by Seo Myung-eung(徐命膺, 1716~1787) are representative. According to "Candongkao(參同攷)", Geon(乾), Gon(坤), Gam(坎), and Ri(離), the four trigrams for divination, each are affiliated with lungs, spleen, kidneys, and heart. Nowadays the study on alchemy correspond with modern trends, which are anti-aging and living without any disease. If processed mineral drugs is assured for stability, it can be used for incurable diseases such as cancer.

An Automated Technique for Illegal Site Detection using the Sequence of HTML Tags (HTML 태그 순서를 이용한 불법 사이트 탐지 자동화 기술)

  • Lee, Kiryong;Lee, Heejo
    • Journal of KIISE
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    • v.43 no.10
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    • pp.1173-1178
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    • 2016
  • Since the introduction of BitTorrent protocol in 2001, everything can be downloaded through file sharing, including music, movies and software. As a result, the copyright holder suffers from illegal sharing of copyright content. In order to solve this problem, countries have enacted illegal share related law; and internet service providers block pirate sites. However, illegal sites such as pirate bay easily reopen the site by changing the domain name. Thus, we propose a technique to easily detect pirate sites that are reopened. This automated technique collects the domain names using the google search engine, and measures similarity using Longest Common Subsequence (LCS) algorithm by comparing the tag structure of the source web page and reopened web page. For evaluation, we colledted 2,383 domains from google search. Experimental results indicated detection of a total of 44 pirate sites for collected domains when applying LCS algorithm. In addition, this technique detected 23 pirate sites for 805 domains when applied to foreign pirate sites. This experiment facilitated easy detection of the reopened pirate sites using an automated detection system.

Metadata Analysis of Open Government Data by Formal Concept Analysis (형식 개념 분석을 통한 공공데이터의 메타데이터 분석)

  • Kim, Haklae
    • The Journal of the Korea Contents Association
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    • v.18 no.1
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    • pp.305-313
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    • 2018
  • Public open data is a database or electronic file produced by a public agency or government. The government is opening public data through the open data portals and individual agency websites. However, it is a reality that there is a limit to search and utilize desired public data from the perspective of data users. In particular, it takes a great deal of effort and time to understand the characteristics of data sets and to combine different data sets. This study suggests the possibility of interlinking between data sets by analyzing the common relationship of item names held by public data. The data sets are collected from the open data portal, and item names included in the data sets are extracted. The extracted item names consist of formal context and formal concept through formal concept analysis. The format concept has a list of data sets and a set of item name as extent and intent, respectively, and analyzes the common items of intent end to determine the possibility of data connection. The results derived from the formal concept analysis can be effectively applied to the semantic connection of the public data, and can be applied to data standard and quality improvement for public data release.