• Asian-Australasian Journal of Animal Sciences
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• 제23권10호
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• pp.1308-1318
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• 2010

The purpose of this study was to evaluate the effect of replacing soybean meal with treated castor meal with (CMT) or without lime (CMNT) on the nutrient intake, performance, carcass characteristics, and yield of commercial cuts of beef cattle from a feedlot. Thirty male, castrated, crossbreed zebu cattle were used in the study, with an average initial weight of $360{\pm}30.27\;kg$. Five animals were used as a control group and were slaughtered at the beginning of the experiment; the remaining animals (n = 25) were distributed in random blocks (repetitions), with body weight as the criterion for block assignment. The animals were fed a diet containing 65% corn silage and 35% of concentrate on dry matter (DM) basis. Five diets consisted of four levels of soybean meal (SM) substituted with CMT (0, 33, 67 and 100%) on a DM basis and a diet with 100% of SM replaced with CMNT. At the end of the experiment, all animals were slaughtered, and their gastrointestinal tracts were emptied to determine their empty body weights (EBW). No significant effects were observed (p>0.05) for the substitution of soybean meal with CMT on intake of dietary nutrients, the average daily body weight gain (ADG) or EBW gain (EBWG). In spite of greater (p<0.05) ricin intake for the diet containing CMNT (3.06 mg/kg BW) compared to the CMT diet (0.10 mg/kg BW/d), there were no effects (p>0.05) on intake of dietary nutrients, ADG or EBWG. The average intake of DM and the ADG were 10,664.63 and 1,353.04 g/d, respectively. Regarding carcass characteristics, only carcass yield in relation to body weight was linearly reduced (p<0.05) upon substitution of SM by CMT. There was no effect (p>0.05) of the substitution of SM by CMT or CMNT on the yield of carcass basic cuts. CMT prices that are higher than 85% of the SM price do not economically justify the use of CMT. For CMT prices between 20 and 80% of the SM price, the optimal level was 67% substitution, while for prices below 15% of the SM price, the optimal level was 100% substitution with CMT. It can be concluded that treated castor meal with 6% lime can totally replace soybean meal in beef cattle diets.

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1553-1558
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• 2012

This study was conducted to investigate the effects of stocking density or group size on feed intake, daily gain, and carcass characteristics of Hanwoo (Korean indigenous breed) steers reared from 7 months to 31 months of age. Thirty Hanwoo steers were divided into four groups with three replicates each (a total of 12 pens). In each group, one (G1), two (G2), three (G3), and four steers (G4) per pen were allocated as treatments. Pen size was $32.0m^2$, and therefore Hanwoo steers in G1, G2, G3, and G4 were reared under different space allowances, i.e. 32.0, 16.0, 10.6, and $8.0m^2$/steer, respectively. Steers were reared following a conventional beef cattle management method in Korea, and were offered a fixed amount of commercial concentrate with ad libitum forages. Results were subjected to analysis of variance with stocking density as the main effect, and significance was declared at p<0.05. Although total feed intake was not significantly altered, it numerically increased in animals of low stocking density (G1) compared to those subjected to high stocking density treatment (i.e. G4). Feed conversion ratio was higher (p<0.05) in G3 compared to G1 and G2. Animals in G1 (low stocking density) grew faster (p<0.05) than those of high stocking density (G3 and G4). Back fat thickness, meat yield index, and meat yield grade were similar among all levels of stocking density. However, longissimus muscle area was larger in G1 and G2 (p<0.01) compared to G3 and G4, and animals in G3 produced smaller carcasses (p<0.05). Carcass quality traits, including marbling score, meat color, fat color, texture, maturity and meat quality grade, as determined by a group of experts, were not significantly different among the treatments. In conclusion, lower stocking density resulted in increased feed efficiency, daily gain, and carcass weight in Hanwoo steers. However it remains unclear whether such differences are the results of stocking density or group size, or a combination of both. Nonetheless, these results confirm previous studies reporting a negative effect of increased stocking density on animal productivity. Further, animal welfare under an intensive farming system in relation to economical return is discussed.

• Asian-Australasian Journal of Animal Sciences
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• 제25권6호
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• pp.806-811
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• 2012

This study was conducted to evaluate effects of plant extracts on methanogenesis and rumen microbial diversity in in vitro. Plant extracts (Artemisia princeps var. Orientalis; Wormwood, Allium sativum for. Pekinense; Garlic, Allium cepa; Onion, Zingiber officinale; Ginger, Citrus unshiu; Mandarin orange, Lonicera japonica; Honeysuckle) were obtained from the Plant Extract Bank at Korea Research Institute of Bioscience and Biotechnology. The rumen fluid was collected before morning feeding from a fistulated Holstein cow fed timothy and commercial concentrate (TDN; 73.5%, crude protein; 19%, crude fat; 3%, crude fiber; 12%, crude ash; 10%, Ca; 0.8%, P; 1.2%) in the ratio of 3 to 2. The 30 ml of mixture, comprising McDougall buffer and rumen liquor in the ratio of 4 to 1, was dispensed anaerobically into serum bottles containing 0.3 g of timothy substrate and plant extracts (1% of total volume, respectively) filled with $O_2$-free $N_2$ gas and capped with a rubber stopper. The serum bottles were held in a shaking incubator at $39^{\circ}C$ for 24 h. Total gas production in all plant extracts was higher (p<0.05) than that of the control, and total gas production of ginger extract was highest (p<0.05). The methane emission was highest (p<0.05) at control, but lowest (p<0.05) at garlic extract which was reduced to about 20% of methane emission (40.2 vs 32.5 ml/g DM). Other plant extracts also resulted in a decrease in methane emissions (wormwood; 8%, onion; 16%, ginger; 16.7%, mandarin orange; 12%, honeysuckle; 12.2%). Total VFAs concentration and pH were not influenced by the addition of plant extracts. Acetate to propionate ratios from garlic and ginger extracts addition samples were lower (p<0.05, 3.36 and 3.38 vs 3.53) than that of the control. Real-time PCR indicted that the ciliate-associated methanogen population in all added plant extracts decreased more than that of the control, while the fibrolytic bacteria population increased. In particular, the F. succinogens community in added wormwood, garlic, mandarin orange and honeysuckle extracts increased more than that of the others. The addition of onion extract increased R. albus diversity, while other extracts did not influence the R. albus community. The R. flavefaciens population in added wormwood and garlic extracts decreased, while other extracts increased its abundance compared to the control. In conclusion, the results indicated that the plant extracts used in the experiment could be promising feed additives to decrease methane gas emission from ruminant animals while improving ruminal fermentation.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1598-1604
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• 2001

Eight $12.4{\pm}0.6kg$ initial body weight crossbred barrows were used to determine the effect of soybean galactooligosaccharides on nutrient and energy digestibility, and digesta transit time. Four dietary treatments were utilized in this trial. Treatment one was a corn-soybean meal based diet (SBM) containing raffinose and stachyose at the levels of 0.16% and 0.75%, respectively. Treatment two (control) was a corn-HP300 (soybean concentrate protein) diet. In treatments three and four, 1.1% and 2.2% commercial stachyose was added to the control diet to provide total dietary stachyose at the levels of 1% and 2%, respectively. The soybean galactooligosaccharides (raffinose + stachyose) level in treatment one was slightly lower compared to that in treatment three. Three collection periods were run with two pigs for each treatment/period. There was a 4 d adjustment period followed by a 3 d collection period. The results showed that the nitrogen retention (86.79%) of pigs fed treatment two diet was higher than that of pigs fed treatment one by 5.2% (p<0.05). The nitrogen retention of treatment three was intermediate 83.09%. The apparent fecal digestibility of all amino acids in treatment two was numerically highest, followed by treatments three and four. However, there were no significant difference among groups (p>0.05). The dry matter (DM), organic matter (OM), crude protein (CP), and crude fiber (CF) digestibility numerically decreased as the soybean galactooligosaccharides level increased, but were not significantly different (p>0.05). Chromium content in feces (from the inclusion of 0.3% chromic oxide in the diets) differed among treatments (p<0.05) at 15 h, 18 h, and 21 h after eating. This showed that the digesta transit time was differed significantly among treatments. Treatment four was the shortest, followed by treatment three, SBM and control. The results demonstrated that in the absence of antinutritional factors and soybean antigen protein, inclusion of 1% and 2% stachyose in corn-HP300 diet has no significant effect on the digestibility of DM, OM, CP, CF and amino acids. When the soybean galactooligosaccharide level in diet one and diet three were adjusted to be almost the same, antinutritional factors such as trypsin inhibitor and soybean antigen protein could decrease the nutrient digestibility and nitrogen retention rate of diet. High levels of soybean galactooligosaccharides shortened the digesta transit time in the intestinal tract. This trial suggested that the total level of soybean galactooligosaccharides (stachyose+raffinose) in the weanling piglet diet is better not to exceed 1% when common soybean meal is used as main protein source.

• Asian-Australasian Journal of Animal Sciences
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• 제24권4호
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• pp.493-499
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• 2011

The objective of this study was to find the way to prolong the storage time of sawdust-based oyster mushroom (Pleurotus osteratus) spent substrate (OMSS) by fermenting with potential probiotic microorganisms to recycle the otherwise waste of mushroom farms. To this purpose, lactic acid bacteria (LAB) were screened to select the best lactic acid-producing strains. Three strains of LAB (Lactobacillus plantarum Lp1', Pediococcus acidilacticii Pa193, L. plantarum Lp2M) were selected and in mixture they lowered the pH of the fermented OMSS to 3.81. fOMSS (fermented sawdust-based oyster mushroom spent substrate) could be stored at room temperature for at least 17 days without any deterioration of feed quality based on the pH, smell, and color. In dry matter disappearance rate in situ, commercial TMR (total mixed ration), OMSS and OMMM (oyster mushroom mycelium mass) showed no significant differences between the samples after 6, 12 and 24 h incubation except for 48 h. Two separate field studies were performed to test the effects of fOMSS supplement on the growth performance of postweaning Holstein calves. Field trials included groups of animals feeding calf starter supplemented with: Control (no supplement), AB (colistin 0.08% and oxyneo 110/110 0.1%), fOMSS (10% fOMSS) and fConc (10% fermented concentrate) and DFM (direct-fed microbials, average $10^9$ cfu for each of three LAB/d/head). Growth performance (average daily gain and feed efficiency) of the fOMSS supplement group was higher than that of AB followed by fConc and DFM even though there was no statistically significant difference. The Control group was lower than any other group. Various hematological values including IgG, IgA, RBC (red blood cell), hemoglobin, and hematocrit were measured every 10 days to check any unusual abnormality for all groups in trial I and II, and they were within a normal and safe range. Our results suggest that sawdust-based OMSS could be recycled after fermentation with three probiotic LAB strains as a feed supplement for post-weaning calves, and fOMSS has the beneficial effects of an alternative to antibiotics for a growth enhancer in dairy calves.

• KSBB Journal
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• 제23권1호
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• pp.37-43
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• 2008

(주)녹십자는 1986년 "훽나인"을 B형 혈우병 치료제로 제조품목허가를 받아 B형 혈우병치료제 공급을 시작하였다. 또한 1991년 New York Blood Center에서 selvent/detergent 바이러스 불활화 방법을 도입하여 제조공정에 추가한 후 혈액유래 바이러스로부터 안전한 제품을 생산하여 왔다. 하지만 이 제품은 혈액응고 제2인자, 제7인자, 제10인자가 함유된 제9인자 복합체로, 정맥 혈전증과 파종성 혈관내응고병증 같은 혈전형성 부작용이 일어날 가능성이 있어, 훽나인보다 순도, 유효성, 바이러스 안전성이 우수한 제품의 개발이 필요하였다. 이를 위해 고순도 제9인자 제제인 "GreenNine VF" 제조공정을 개발하였다. GreenNine VF 제조공정은 기존의 훽나인 생산 공정에 heparin 친화성 크로마토그래피와 양이온 크로마토그래피가 추가된 공정으로, 바이러스 안전성을 증진시키기 위한 바이러스 필터 공정도 포함하고 있다. 이러한 공정에 의해서 산업적 규모로 생산된 GreenNine VF는 훽나인에 비해 순도와 바이러스 안전성이 월등히 높은 것으로 확인되었다. 또한 고순도 혈액응고 제9인자 제제인 Mononine, Octanyne, Berinin HS, Immunine STIM plus 600보다 순도가 더 높았다. Cryo-poor plasma 1,600 L를 원료로 사용했을 때 1 batch 당 250IU 분병제품 2,400병 이상, 500 IU 분병제품 1,200병 이상을 생산할 수 있었다.

• 한국환경생태학회지
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• 제24권2호
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• pp.101-107
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• 2010

본 연구에서는 태안해안국립공원을 탐방한 이용자들을 대상으로 설문조사를 통한 이용 후 평가를 통하여 국립공원 탐방에 대한 중요도와 만족도, 만족요인, 만족도 예측모형 분석을 통하여 국립공원관리의 기초자료를 제공하고자 하였다. 중요도-성취도 분석결과, 호객행위나 잡상행위, 고성방가, 무분별한 이용, 불법포획 및 식물채취 등에 대한 만족도가 높게 나타났으며, 중요도 항목은 쓰레기 투기 문제, 수질오염행위, 무분별한 취사행위, 바가지요금 등이 높은 값을 보였다. 특산물 및 기념품, 공원이용프로그램, 공공시설, 공원이용정보시설, 상업시설 등의 항목은 '우선시정'을 필요로 하는 즉, 집중적인 관리가 필요한 항목이었으며, 나머지 항목은 '지속적 노력'이 필요한 항목으로 나타났다. 요인분석 결과, '시설관리' '이용관리' '자원관리'요인 순으로 추출되었으며, '시설관리요인'의 설명력이 가장 높게(32.6%) 나타나 시설관리의 질적 수준이 만족도를 결정하는 중요한 요인임을 알 수 있었다. 만족도 예측모형에서는 '관리자의 친절 및 안내' '편의시설부족' '고성방가' '숙박시설 부족' '이용요금 및 주차비'등이 만족도에 영향을 미치는 변수로 나타나, 이들 요소에 대한 집중적인 관리가 요구된다 하겠다. 이와 같은 결과는 해안공원의 특성이 반영된 것으로 산악형 국립공원과는 차별화된 관리전략과 정책이 필요하다 하겠다.

• 멤브레인
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• 제17권4호
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• pp.338-344
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• 2007

HI몰랄리티가 9.5 $mol/kg-H_2O$인 HI의 전해-전기투석을 시판의 양이온교환막(CMB)을 이용하여 요오드의 존재하에 실험을 진행하였다. 수소이온 투과의 선택성을 증가시키기 위해, 막은 전자선 가속기를 이용하여 방사선 처리하였다. 방사선 처리한 막의 막특성(막 저항, 이온교환용량, 함수율)을 측정하였다. 각각의 방사선량에서 처리한 막의 2 $mol/dm^3$의 KCl 용액에서 막저항, 이온교환용량과 함수율은 처리하지 않은 막과 거의 동등의 값을 가졌다. HI몰랄리티가 9.5 $mol/kg-H_2O$인 HI의 전해-전투기투석을 $75^{\circ}C$, 9.6 $A/dm^2$에서 진행하였다. 전자선 가속기에 의해 방사선 처리한 양이온교환막은 처리하지 않은 막과 비교하여 고분자의 가교구조와 함께 수소이온투과의 높은 선택성을 가졌다.

• 한국응용곤충학회지
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• 제56권4호
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• pp.403-412
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• 2017

춘천지역 농가 및 강원도농업기술원 내 시험포장에서 반촉성재배토마토(2~6월)에서 트랩을 이용한 주요 해충의 발생밀도와 천적 등을 이용한 방제효과를 조사 하였다. 토마토 재배농가에서 주로 사용하는 약제와 유기농자재의 천적곤충에 대한 독성평가도 수행하였다. 반촉성재배 시 발생되는 주요 해충은 온실가루이(Trialeurodes vaporariorum)와 총채벌레류이었다. 온실가루이의 경우 4월 중순경부터 발생하기 시작하여 5월 중순 이후 발생이 증가하였고, 총채벌레류는 4월 상순 경 발생을 시작하여 6월 이후에 증가하는 경향을 보였다. 저독성약제 선발시험을 통해 온실가루이와 총채벌레의 천적인 온실가루이좀벌과 미끌애꽃노린재에 대해 60% 이하의 독성을 보이는 살충제 1종(spiromesifen SC), 살균제 1종(cyazofamid SC) 및 님 추출물이 함유된 유기농자재 외 1종을 선발하였다. 이상의 결과에서 제시한 천적중심 관리모델을 적극적으로 활용한다면 토마토 반촉성 시설재배 시 온실가루이와 총채벌레를 효율적으로 관리할 수 있을 것으로 기대된다.

• 한국정보과학회논문지:정보통신
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• 제37권6호
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• pp.420-430
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• 2010

Vehicular Ad Hoc Network(VANET)은 차량과 차량, 차량과 네트워크 기반 구조 간에 통신을 지완하는 네트워크로서, 다양한 서비스를 제공할 수 있어 학문적으로나 상업적으로 많은 관심을 받고 있다. 그러나 VANET이 널려 사용되기 위해서는 보안과 프라이버시 관련 문제들이 선행적으로 해결되어야 한다. VANET은 무선 통신과 ad hoc network의 성질을 그대로 이어받아, 다양한 보안 취약점이 존재하며 이에 따라 다양한 공격이 가능하다. 또한, 프라이버시 요구사항을 만족시키지 못하는 경우, 공격자는 특정 차량에 때해서 추적할 수 있으며, 운전자의 민감한 개인정보들이 노출될 수 있다. VANET에서 프라이버시와 보안을 제공하기 위해 많은 연구들이 진행되었지만, 대부분이 차량 간의 통신 혹은 차량과 네트워크 기반 구조 간의 통신 중 하나에만 집중하고 있으며, 차량의 폐지목록(Revocation List)으로 인해 많은 네트워크 자원을 소비하고 있다. 본 논문에서는 차량과 네트워크 기반구조간의 효율적인 상호 인증을 제공하고, 상호인증 시 네트워크 기반 구조가 차량에게 짧은 시간 동안 차량과 차량 간의 통신에서 사용할 익명 인증서를 생성해주는 프로토콜을 제안한다. 보안 분석을 통해 제안한 프로토콜이 차량과 차량, 차량과 네트워크 기반 구조간의 안전한 통신을 보장하고, 차량의 프라이버시를 보호할 수 있음을 확인 하였으며, 성능 분석을 통해 제안한 프로토콜이 기존의 연구들 보다 높은 효율성을 지니고 있음을 검증하였다.