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Enrichment of $CO_2$-Fixing Bacteria in Cylinder-Type Electrochemical Bioreactor with Built-In Anode Compartment

  • Jeon, Bo-Young;Jung, Il-Lae;Park, Doo-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.21 no.6
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    • pp.590-598
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    • 2011
  • Bacterial assimilation of $CO_2$ into stable biomolecules using electrochemical reducing power may be an effective method to reduce atmospheric $CO_2$ without fossil fuel combustion. For the enrichment of the $CO_2$-fixing bacteria using electrochemical reducing power as an energy source, a cylinder-type electrochemical bioreactor with a built-in anode compartment was developed. A graphite felt cathode modified with neutral red (NR-graphite cathode) was used as a solid electron mediator to induce bacterial cells to fix $CO_2$ using electrochemical reducing power. Bacterial $CO_2$ consumption was calculated based on the variation in the ratio of $CO_2$ to $N_2$ in the gas reservoir. $CO_2$ consumed by the bacteria grown in the electrochemical bioreactor (2,000 ml) reached a maximum of approximately 1,500 ml per week. Time-coursed variations in the bacterial community grown with the electrochemical reducing power and $CO_2$ in the mineral-based medium were analyzed via temperature gradient gel electrophoresis (TGGE) of the 16S rDNA variable region. Some of the bacterial community constituents noted at the initial time disappeared completely, but some of them observed as DNA signs at the initial time were clearly enriched in the electrochemical bioreactor during 24 weeks of incubation. Finally, Alcaligenes sp. and Achromobacter sp., which are capable of autotrophically fixing $CO_2$, were enriched to major constituents of the bacterial community in the electrochemical bioreactor.

Comparison of Model-simulated Atmospheric Carbon Dioxide with GOSAT Retrievals

  • Shim, Chang-Sub;Nassar, Ray;Kim, Jhoon
    • Asian Journal of Atmospheric Environment
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    • v.5 no.4
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    • pp.263-277
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    • 2011
  • Global atmospheric $CO_2$ distributions were simulated with a chemical transport model (GEOS-Chem) and compared with space-borne observations of $CO_2$ column density by GOSAT from April 2009 to January 2010. The GEOS-Chem model simulated 3-D global atmospheric $CO_2$ at $2^{\circ}{\times}2.5^{\circ}$ horizontal resolution using global $CO_2$ surface sources/sinks as well as 3-D emissions from aviation and the atmospheric oxidation of other carbon species. The seasonal cycle and spatial distribution of GEOS-Chem $CO_2$ columns were generally comparable with GOSAT columns over each continent with a systematic positive bias of ~1.0%. Data from the World Data Center for Greenhouse Gases (WDCGG) from twelve ground stations spanning $90^{\circ}S-82^{\circ}N$ were also compared with the modeled data for the period of 2004-2009 inclusive. The ground-based data show high correlations with the GEOS-Chem simulation ($0.66{\leq}R^2{\leq}0.99$) but the model data have a negative bias of ~1.0%, which is primarily due to the model initial conditions. Together these two comparisons can be used to infer that GOSAT $CO_2$ retrievals underestimate $CO_2$ column concentration by ~2.0%, as demonstrated in recent validation work using other methods. We further estimated individual source/sink contributions to the global atmospheric $CO_2$ budget and trends through 7 tagged $CO_2$ tracers (fossil fuels, ocean exchanges, biomass burning, biofuel burning, net terrestrial exchange, shipping, aviation, and CO oxidation) over 2004-2009. The global $CO_2$ trend over this period (2.1 ppmv/year) has been mainly driven by fossil fuel combustion and cement production (3.2 ppmv/year), reinforcing the fact that rigorous $CO_2$ reductions from human activities are necessary in order to stabilize atmospheric $CO_2$ levels.

Biochemical and Molecular Characterization of Laccases from Wild Mushrooms

  • Ro, Hyeon-Su
    • 한국균학회소식:학술대회논문집
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    • 2014.05a
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    • pp.43-43
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    • 2014
  • White rot fungi have been useful source of enzymes for the degradation of environmental pollutants including polycyclic aromatic hydrocarbons (PAHs) and synthetic dyes. PAHs are widespread organic compounds present in fossil fuels and are routinely generated by incomplete fuel combustion. PAHs are some of the major toxic pollutants of water and soil environments. Synthetic dyes are major water-pollutants, which are toxic to organisms in water environments and interfere photosynthesis of water plants. Removal of PAHs and synthetic dyes has been of interests in the environmental science especially in the environmental microbiology. Mushrooms are fungal groups that function as primary degraders of wood polyphenolic lignin. The ligninolytic enzymes produced by mushroom, including manganese peroxidase, lignin peroxidase, and laccase, mediate the oxidative degradation of lignin. The catalytic power of these enzymes in the degradation of aromatic ring compounds has been sought for the degradation of various organic compounds. In this project, we have screened 60 wild mushroom strains for their degradation activity against two representative PAHs, naphthalene and anthracene, and five aromatic dyes, including alizarin red S, crystal violet, malachite green, methylene blue, rose bengal. The degradation of PAHs was measured by GC while the decolorization of dyes was measured by both UV spectrophotometer and HPLC. As results, 9 wild mushroom strains showed high activity in degradation of PAHs and textile dyes. We also describe the secretive enzyme activities, the transcription levels, and cloning of target genes. In conjunction with this, activities of degradative enzymes, including laccase, lignin peroxidase, and Mn peroxidase, were measured in the liquid medium in the presence of PAHs and dyes. Our results showed that the laccase activity was directed correlated with the degradation, indicating that the main enzyme acts on PAHs and dyes is the laccase. The laccase activity was further simulated by the addition of $Cu^{2+}$ ion. Detailed studies of the enzyme system should be sought for future applications.

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A study of improving filtration efficiency through SiC whisker synthesis on carbon felt by CVD VS method

  • Kim, Gwang-Ju;Choe, Du-Jin
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.150-150
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    • 2016
  • Mankind is enjoying a great convenience of their life by the rapid growth of secondary industry since the Industrial Revolution and it is possible due to the invention of huge power such as engine. The automobile which plays the important role of industrial development and human movement is powered by the Engine Module, and especially Diesel engine is widely used because of mechanical durability and energy efficiency. The main work mechanism of the Diesel engine is composed of inhalation of the organic material (coal, oil, etc.), combustion, explosion and exhaust Cycle process then the carbon compound emissions during the last exhaust process are essential which is known as the major causes of air pollution issues in recent years. In particular, COx, called carbon oxide compound which is composed of a very small size of the particles from several ten to hundred nano meter and they exist as a suspension in the atmosphere. These Diesel particles can be accumulated at the respiratory organs and cause many serious diseases. In order to compensate for the weak point of such a Diesel Engine, the DPF(Diesel Particulate Filter) post-cleaning equipment has been used and it mainly consists of ceramic materials(SiC, Cordierite etc) because of the necessity for the engine system durability on the exposure of high temperature, high pressure and chemical harsh environmental. Ceramic Material filter, but it remains a lot of problems yet, such as limitations of collecting very small particles below micro size, high cost due to difficulties of manufacturing process and low fuel consumption efficiency due to back pressure increase by the small pore structure. This study is to test the possibility of new structure by direct infiltration of SiC Whisker on Carbon felt as the next generation filter and this new filter is expected to improve the above various problems of the Ceramic DPF currently in use and reduction of the cost simultaneously. In this experiment, non-catalytic VS CVD (Vapor-Solid Chemical Vaporized Deposition) system was adopted to keep high mechanical properties of SiC and MTS (Methyl-Trichloro-Silane) gas used as source and H2 gas used as dilute gas. From this, the suitable whisker growth for high performance filter was observed depending on each deposition conditions change (input gas ratio, temperature, mass flow rate etc.).

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A Study of GHG-AP Integrated Inventories and Alternative Energy Use Scenario of Energy Consumption in the University (대학 내 에너지 소비에 따른 온실가스-대기오염 통합 인벤토리 및 대체 에너지 사용 시나리오 분석)

  • Jung, Jae-Hyung;Kwon, O-Yul
    • Journal of Environmental Science International
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    • v.23 no.9
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    • pp.1643-1654
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    • 2014
  • The university is one of the main energy consumption facilities and thereby releases a large amount of greenhouse gas (GHG). Accordingly, efforts for reducing energy consumption and GHG have been established in many local as well as international universities. However, it has been limited to energy consumption and GHG, and has not included air pollution (AP). Therefore, we estimated GHG and AP integrated emissions from the energy consumed by Seoul National University of Science and Technology during the years between 2010 and 2012. In addition, the effect of alternative energy use scenario was analysed. We estimated GHG using IPCC guideline and Guidelines for Local Government Greenhouse Inventories, and AP using APEMEP/EEA Emission Inventory Guidebook 2013 and Air Pollutants Calculation Manual. The estimated annual average GHG emission was $11,420tonCO_{2eq}$, of which 27% was direct emissions from fuel combustion sectors, including stationary and mobile source, and the remaining 73% was indirect emissions from purchased electricity and purchased water supply. The estimated annual average AP emission was 7,757 kgAP, of which the total amount was from direct emissions only. The annual GHG emissions from city gas and purchased electricity usage per unit area ($m^2$) of the university buildings were estimated as $15.4kgCO_{2eq}/m^2$ and $42.4tonCO_{2eq}/m^2$ and those per person enrolled in the university were $210kgCO_{2eq}$/capita and $577kgCO_{2eq}$/capita. Alternative energy use scenarios revealed that the use of all alternative energy sources including solar energy, electric car and rain water reuse applicable to the university could reduce as much as 9.4% of the annual GHG and 34% of AP integrated emissions, saving approximately 400 million won per year, corresponding to 14% of the university energy budget.

Current Status and Technical Development for Di-Methyl Ether as a New and Renewable Energy (신재생 에너지로서 DME 기술개발 현황)

  • Cho, Wonjun;Kim, Seung-Soo
    • Applied Chemistry for Engineering
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    • v.20 no.4
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    • pp.355-362
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    • 2009
  • Fuels based on petroleum will eventually run out in the near future. DME (Di-methyl Ether) is a clean energy source that can be manufactured from various raw materials such as natural gas, coal as well as biomass. As DME has no carbon-carbon bond in its molecular structure and is an oxygenate fuel, its combustion essentially generates no soot as well as no SOx. Because the physical properties of DME are similar to those of LPG, the LPG distribution infrastructure can be converted to use with DME. DME has such high cetane number of 55~60 that it can be used as a diesel engine fuel. Practical use of DME as a next-generation clean fuel or next-generation chemical feedstock is advancing in the fields of power generation, diesel engines, household use, and fuel cells, among others. The purpose of this paper is review the characteristics, standardization, status of research and development in domestic and foreign countries of DME.

Application and verification analysis of the thermoelectric generation system for a coastal fishing vessel (열전발전 장치의 연안어선 활용에 관한 연구)

  • LEE, Donggil;YANG, Yongsu;KIM, Pyungkwan;KIM, Seonghun;KIM, Hyunyoung
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.53 no.2
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    • pp.196-203
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    • 2017
  • The renewable energy sources can be thought of one of the major measures to reduce greenhouse gas emissions in the industries. However, the utilization technology for those sources is approaching in the different matters. One of them, thermoelectric generation might be applicable to fishing industry. A various of internal combustion engines are used in a wide range of fisheries. After the ignition process, the heat passed out from the exhaust outlet. Recycling the heat could be not only an energy source but also reduction of green gas emission. Therefore, this study was designed to verify the feasibility of generation from wasted exhaust gas and analyze the performance. The designed experiment devices were connected with a data logger and an electric loader to quantify the currency and voltage. The devices were installed in a coastal fishing vessel for a gillnet fishery. During the whole fishing trips, the amount of generation was measured by engine rpm and the fishing operation procedures including vessel operations. At the maximum 1,500 rpm in the practical range, the generation amount was 113.6 W. The amount difference in relation to connection method was within 5 W: serial connection was 111.4 W and parallel connection was 115.8 W.

Chemical Characteristics of Ambient Aerosol during Asian Dusts and High PM Episodes at Seoul Intensive Monitoring Site in 2009 (2009년 서울지역 황사 및 고농도 미세먼지 사례 시 미세먼지의 화학성분 특성)

  • Park, Seung-Myung;Moon, Kwang-Joo;Park, Jong-Sung;Kim, Hyun-Jae;Ahn, Jun-Yong;Kim, Jeong-Soo
    • Journal of Korean Society for Atmospheric Environment
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    • v.28 no.3
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    • pp.282-293
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    • 2012
  • This study aimed to show the chemical characteristics of $PM_{10}$ and $PM_{2.5}$ during Asian dust (AD) events and high PM episodes observed in Seoul. Time-resolved chemical composition of $PM_{10}$ or $PM_{2.5}$ was monitored in 1 hour interval using ambient ion monitor, semi-continuous carbon monitor, and on-line XRF spectrometer at Seoul intensive monitoring site in 2009. Considering that AD events were classified into three different cases according to the source area and pathway, the concentrations of ammonium-sulfate and trace metal components were relatively high when the AD were occurred in Gobi and inner-mongolia and transported across the Bohai bay. In addition, the influence of alkaline dust, which carried from saline land located in the northeastern China, was observed when the AD was originated from Gobi, inner-mongolia, and Manchuria. Except AD events, the high PM episodes observed in Seoul were impacted by various anthropogenic sources such as biomass burning, motor vehicle, oil combustion and road dust.

Characterization of Urease-Producing Bacteria Isolated from Heavy Metal Contaminated Mine Soil

  • Park, Min-Jeong;Yoon, Min-Ho;Nam, In-Hyun
    • Korean Journal of Soil Science and Fertilizer
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    • v.47 no.6
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    • pp.391-397
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    • 2014
  • Acid mine drainage occurrence is a serious environmental problem by mining industry; it usually contain high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of greatest concern. It causes mine impacted soil pollution with mining and smelting activities, fossil fuel combustion, and waste disposal. In the present study, three bacterial strains capable of producing urease were isolated by selective enrichment of heavy metal contaminated soils from a minei-mpacted area. All isolated bacterial strains were identified Sporosarcina pasteurii with more than 98% of similarity, therefore they were named Sporosarcina sp. KM-01, KM-07, and KM-12. The heavy metals detected from the collected mine soils containing bacterial isolates as Mn ($170.50mg\;kg^{-1}$), As ($114.05mg\;kg^{-1}$), Zn ($92.07mg\;kg^{-1}$), Cu ($62.44mg\;kg^{-1}$), and Pb ($40.29mg\;kg^{-1}$). The KM-01, KM-07, and KM-12 strains were shown to be able to precipitate calcium carbonate using urea as a energy source that was amended with calcium chloride. SEM-EDS analyses showed that calcium carbonate was successfully produced and increased with time. To confirm the calcium carbonate precipitation ability, urease activity and precipitate weight were also measured and compared. These results demonstrate that all isolated bacterial strains could potentially be used in the bioremediation of acidic soil contaminated by heavy metals by mining activity.

An Experimental Study on Annulus Muffler of Automobile (자동차용 환상형 소음기에 관한 실험적 연구)

  • Kim, Byoung-Sam;Song, Kyu-Keun;Sim, Sang-Cherl;Cheong, Byeong-Kuk
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2006.11a
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    • pp.217-222
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    • 2006
  • Internal combustion engine is the main source of environmental pollutants and therefore advanced technology is required to reduce harmful elements from the exhaust gases all over the world. Especially, when the exhaust gas is released front the automotive muffler, exhaust noise has many bad influence on the surrounding environment. In order to reduce the exhaust noise, it is necessary that automotive muffler must be designed for best exhaust efficiency. The sound insulation room was installed for the analysis of an acoustics characteristics of the noise from automotive muffler, in this study. Exhaust gas noise, noise distribution characteristics, pressure and temperature of exhaust gas were investigated with the change of annulus temperature of air cooled annulus automotive muffler and cooled annulus automotive muffler. The following results were obtained with this study. From the frequency analysis of automotive muffler, high noise distribution was observed in the range $100{\sim}2000Hz$. It means that the noise in this range has an dominate influence for the overall noise. Noise reduction of automotive muffler was affected by the temperature of annulus. It is caused the result that the high temperature and pressure of exhaust gas are changed lower by the drop of annulus temperature. The tendencies of noise, the temperature and pressure of exhaust gas are similar to the performance curve of engine. Exhaust gas pressure is determined by the r.p.m. of engine and affected by the cooling performance of automotive muffler.

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