• Asian-Australasian Journal of Animal Sciences
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• v.20 no.12
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• pp.1887-1894
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• 2007

In this paper, a rapid and reliable gene-targeted species-specific polymerase chain reaction (PCR) technique based on a two-step process was established to identify bifidobacteria in dairy products. The first step was the PCR assay for genus Bifidobacterium with genus specific primers followed by the second step, which identified the species level with species-specific primer mixtures. Ten specific primer pairs, designed from nucleotide sequences of the 16-23S rRNA region, were developed for the Bifidobacterium species including B. angulatum, B. animalis, B. bifidum, B. breve, B. catenulatum, B. infantis, B. longum, B. minimum, B. subtile, and B. thermophilum. This technique was applied to the identification of Bifidobacterium species isolated from 6 probiotic products, and four different Bifidobacterium spp. (B. bifidum, B. longum, B. infantis, and B. breve) were identified. The findings indicated that the 16S-23S rDNA gene-targeted species-specific PCR technique is a simple and reliable method for identification of bifidobacteria in probiotic products. PCR combined with Denaturing Gradient Gel Electrophoresis (DGGE) for identification of the bifidobacteria was also evaluated and compared with the gene-targeted species-specific technique. Results indicated that for fermented milk products consistency was found for both species-specific PCR and PCR-DGGE in detecting species. However, in some lyophilized products, the bands corresponding to these species were not visualized in the DGGE profile but the specific PCR gave a positive result.

• Journal of TMJ Balancing Medicine
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• v.4 no.1
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• pp.8-11
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• 2014

A 27 year-old male patient with sleep bruxism-induced temporomandibular pain was managed by appliance of FCST with 3 weeks of duration, combined with acupuncture. After being treats for 3 weeks, the patient's pain was improved. Assessment was made by self assessment (Numerical Rating Scale, NRS; Korean Oral Health Impact Profile, KOHIP) and clinical observation. An impressive effect was observed and further clinical and biological research on FCST is expected.

• 유체기계공업학회:학술대회논문집
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• 1998.02a
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• pp.128-135
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• 1998

Procedures and results of aerodynamic design of a centrifugal compressor are presented for development of a 40kW class turbogenerator gas turbine. Specification of higher level of total pressure ratio of 4 and total efficiency of $80\%$ requires advanced methods of design and analysis. In the meanline design/analysis, a method with conventional loss modeling and a method with the two-zone model are alternately used for more reliable prediction. In the impeller blade generation, a series of Bezier curve are combined to produce meridional contours and distributions of blade camber angle and blade thickness. Intermediate profiles of blades are repeatedly produced and changed to be finally fixed through quasi-three dimensional Euler flow analysis. Three dimensional compressible turbulent flow analysis is then performed for the impeller to be confirmed in the final step of design. Satisfactory results in the aerodynamic performance are obtained, which assures that there is no need of aerodynamic re-design.

• Journal of Welding and Joining
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• v.16 no.2
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• pp.111-121
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• 1998

It is well recognized that a excessive temperature gradient from the junction of head to skirt in axial direction in a hot pressure vessel can cause unpredicted high thermal stress at the junction and/or in axial direction of a skirt. this thermal stress resulting from axial thermal gradient may be a major cause of unsoundness of structural integrity. In case of cyclic operation of hot pressure vessels, the thermal stress becomes one of the primary design consideration because of the possibility of fracture as a result of cyclic thermal fatigue and progressively incremental plastic deformation. To perform thermal stress analysis of the junction and cylindrical skirt of a vessel, or, at least, to inspect quantitatively the magnitude and effect of thermal stress, the temperature profile of the vessel and skirt must be known. This paper demonstrated the temperature distribution and thermal stress analysis for the junction of skirt to head using F.E. analysis. Effect of air pocket in crotch space was quantitatively investigated to minimize the temperature gradient causing the thermal stress in axial direction. Effect of the skirt height on thermal stresses was also studied. Analysis results were compared with theoretical formulas to verify th applicability to the strength calculation in design field.

• Food Science and Biotechnology
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• v.18 no.1
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• pp.36-42
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• 2009

This study was performed to develop low-fat/reduced-salt sausages (LFRSS; <3% fat and <1.5% salt) containing milk protein (whey protein concentrate, WPC, or sodium caseinate, SC) that showed the similar cooking yield and textural characteristics to those of regular-fat/salt sausage control (RFC; 20% fat and 1.5% salt) or low-fat sausage control (LFC; <3% fat and 1.5% salt). Low-fat sausages (LFS) were formulated with a 2.5% fat replacer (konjac flour:carrageenan:soy protein isolate=1:1:3) and various salt levels (0.75, 1.0, 1.25, and 1.5%). LFS had differences in color and expressible moisture (EM, %) values as compared to those of RFC. A minimum salt level of 1% and addition of nonmeat proteins were required to manufacture LFRSS that have similar characteristics to those of RFC. However, LFS with 2% milk proteins reduced the hardness and gumminess as compared to LFC. These results indicated that 1% milk protein in combined with 1% salt was a proper level for manufacturing of LFRSS.

• Structural Engineering and Mechanics
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• v.41 no.4
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• pp.509-525
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• 2012

In recent years, CFRP material usage in strengthening applications gradually became widespread. Especially, the studies on the strengthening of shear deficient reinforced concrete beams with CFRP strips are chosen as a subject to numerous experimental studies and research on this subject are increased rapidly. The most important variable, that is affected on the failure mode of CFRP strips and that is needed for determining the shear capacity of the strengthened reinforced concrete beams, is the strain distribution between CFRP strips and concrete. Numerous experimental studies are encountered in the literature about the determination of strain distribution between CFRP strips and concrete. However, these studies mainly focused on the CFRP strips under axial tension. There are very limited numbers of experimental and analytic studies examining the strain distribution between concrete and CFRP strips, which are under combined stresses due to the effects of shear force and bending moment. For this reason, existing experimental study in the literature is used as model for ANSYS finite element software. Nonlinear finite element analysis of RC beams strengthened against shear with CFRP strips under reverse cyclic loading is performed. The strain distributions between CFRP strips and concrete that is obtained from finite element analysis are compared with the results of experimental measurements. It is seen that the experimental results are consisted with the results derived from the finite element analysis and important findings on the strain distribution profile are reached by obtaining strain values of many points using finite element method.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.19 no.12
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• pp.811-820
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• 2007

A Study on the optimal design of the AIG(Ammonia Injection Grid) to improve the performance of DeNOx facilities in the HRSG(Heat Recovery Steam Generator) was performed using the CFD analysis. On the basis of the flow analysis results in the case that the AIG in the HRSG was not installed, the numerical analyses according to the positions of AIG, injection angles of nozzle and the control of ammonia injection quantity were carried out. The standard deviation according to factors was calculated for quantitative comparison. As the results, the AIG in the HRSG should be installed in the position that the uniform flow field shows through the exact flow analysis in the previous of the AIG design and installation. In the case the AIG has already been installed and non uniform flow distribution shows, it is recommended that flow correction device or KoNOx catalyst should be used. Otherwise, the control of ammonia injection angle or the ammonia injection quantity using the velocity profile analysis is demanded to accomplish the optimal performance.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.11 no.10
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• pp.796-803
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• 1998

Intrinsic and proximity gettering are popular processes to get higher cumulative production yield and usually adopt multi-step annealing and high energy ion implantation, respectively. In order to test the combined processed of these, high energy \ulcornerAs\ulcorner ion implantation and 2-step annealing process were adopted. After the ion implantation followed by 2-step annealing, the wafers were cleaved and etched with Wright etchant. The morphology of cross section on samples was inspected by FESEM. The concentration profile of As was measured by SRP. The location and type of secondary defects inspected by HRTEM were dependent on the 1st annealing temperatures. That is, a line of dislocation located at $1.5mutextrm{m}$ apart from the surface at $600^{\circ}C$ lst annealing was changed to some dislocation lines or loops nearby the surface at 100$0^{\circ}C$. The density of dislocation line was reduced but the size of the defects was enlarged as the temperature increased.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.23 no.7
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• pp.841-855
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• 1999

In the present work, the effect of a flow maldistribution on the thermal and conversion response of 8 monolith catalytic converter is Investigated. To achieve this goal, a combined chemical reaction and multi-dimensional fluid dynamic mathematical model has been developed. The present results show that flow uniformity within the monolith brick has 8 great impact on light-off performance of the catalytic converter. In the case of lower flow uniformity, large portions of the monolith remain cold due to locally concentrated high velocities and CO, HC are unconverted during warm-up period, which loads to retardation of light-off. It has been also found that the heat-up pattern of the monolith ill similar to the flow distribution profile, In the early stage of the reaction. It may be concluded that flow maldistribution can cause a significant retardation of the light-off and hence can eventually worsen tho conversion efficiency of automotive catalytic converter.

• Journal of the Society of Naval Architects of Korea
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• v.47 no.6
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• pp.813-820
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• 2010

The spread mooring system for FPSO is developed to explore deep sea area, in which swell is dominant. It is known that the tension response of mooring lines in this sea area shows bimodal spectrum. Assuming normal distribution of tension profile and Rayleigh distribution of tension amplitude, the power spectral density function (PSD) of the mooring tension under the bimodal stationary random process is applied for the calculation of spectrum fatigue. Three popular methods, which are simple summation method, combined spectrum method and Jioa-Moan method, are used to combine fatigue damages from bimodal spectrum characteristics. Each damage value is compared with damage using Rainflow Cycle Counting (RCC) method which is believed to be close to exact solution. Vanmarcke' parameter and RMS(Root Mean Square) ratio are employed to assess relative damage variations between from RCC method and from three combination methods. Finally the most reliable fatigue damage combining method for spread mooring system is suggested.