• Title/Summary/Keyword: Combinations

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Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay

  • Kim, Me-Sun;Ouk, Sothea;Jung, Kuk-Hyun;Song, Yoohan;Le, Van Trang;Yang, Ju-Young;Cho, Yong-Gu
    • Plant Breeding and Biotechnology
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    • v.7 no.3
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    • pp.272-286
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    • 2019
  • Developing elite hybrid rice varieties is one important objective of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F1 hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F1 hybrid combinations and two control vatieties. Results showed that nine F1 hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F1 hybrid rice varieties with resistance to disease/insect were selected from eleven F1 hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F1 hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

Eight Confluent Acupoint Combinations Patterns: Data Mining and Network Analysis (데이터마이닝과 네트워크분석을 통한 팔맥교회혈의 배합 패턴 연구)

  • Min-Jeong Kwon;Da-Eun Yoon;Heeyoung Moon;Yeonhee Ryu;In-Seon Lee;Younbyoung Chae
    • Korean Journal of Acupuncture
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    • v.40 no.4
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    • pp.177-183
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    • 2023
  • Objectives : One of the crucial combinations of acupoints for treating various disorders involves the Eight Confluent acupoints. The present study aims to investigate the selection patterns of the Eight Confluent acupoints in clinical trials and determine the most frequent pairings through network analysis. Methods : The frequencies of the Eight Confluent acupoints were extracted from the Acusynth database, which includes data from 421 clinical investigations. We examined the degree distribution, eigenvector centrality, proximity centrality, and betweenness centrality of these acupoint combinations using network analysis. Results : Data mining revealed that among the Eight Confluent acupoints, PC6 and TE5 were the most commonly applied in the treatment of 30 disorders. Additionally, we identified the most frequently co-occurring pairs of Eight Confluent acupoints by network analysis which included PC6-GV20, SP4-GV4, LU7-LI4, TE5-PC7, GB41-SP6, KI6-BL62, and SI3-BL62. Conclusions : Through the application of data mining and network analysis, we have elucidated the selection patterns and combinations of the Eight Confluent acupoints. These findings provide valuable insights that can enhance doctors' understanding of clinical database-driven Eight Confluent acupoint selection patterns.

Combination of Natural Products Removing ROS for Growth Promoting Effects of the Useful Enterobacteria Lacobacillus sp. (ROS 제거기능을 갖는 천연산물로부터 장내 유용세균 Lacobacillus속의 성장을 촉진시키는 조합의 구성)

  • 김종덕;김민용;서효진;김봉조;김대현;김은옥;정해영;공재열
    • Microbiology and Biotechnology Letters
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    • v.30 no.3
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    • pp.270-281
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    • 2002
  • The growth of Enterobacteria, Lactobacillus acidophilus and L. plantarum was promoted by natural products themselves bearing antioxidative capacity and combined two, three and four kinds of them.1. acidophilus was promoted by Paeonia japonica, Acori graminei rhizoma, Aurantii nobilis pericarpium, alone, and two mixed combinations were composed of Acori graminei rhizoma and Cinnamomi cortex, Aurantii nobilis pericarpium and Puerariae radix, and three mixed combinations were formed with Cimicifugae rhi- zoma, Cinnamomi cortex and Glycyrrhizae radix, and four mixed combinations were made up Glycyrrhizae radix, Theae folium, Scutellaria baicalensis george and Cinnamomi cortex. There four combination promoted growth of 1. acidophilus with 1.3 times than that of control, and their antioxidative capacity also higher than that off times, the ratio of elimination of hydroxyl radical was more than 70% in dilution rate of 100 times. In the case of 1. plantarum was premoted by Corm fructus, Nelumbo nuclfera gaertner Sophorae flos, alone, and two mixed combinations were organized with Schizandrae Iructus and Theae folium, Paeonia japonica and Epimedii herba, and three mixed combinations were combined with Corni fructus and Theae folium and Paeonia japonica, Corni fructus and Coptidis rhizoma and Schieandrae fructus, and four mixed combinations were composed of Corm fructus, Theae folium, Coptidis rhizoma, Scutellaria baicalensis george, and Corm fructus, Schizandrae fructus, Paeonia japonica, Theae folium. These four combinations assisted growth of 1. plantarum with 1.5 times than that of control, and their antioxidative capacity also higher than that of 4-5 times, the ratio of elimination of hydroxyl radical was more than 75% in dilution rate of 100 times. As these combinations of natural products could activate some parts of body, they might be applied pharmaceuitcal sources, functional foods, and expected to fermentative beverages.

Classification of Rice Cultivar Using Two-dimensional Ordination Analysis (Two-dimensional Ordination 분석법에 의한 수도 품종 분류)

  • Soon-Chul Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.1
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    • pp.74-80
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    • 1989
  • Twentyseven rice cultivars were compared Quantitatively and classified by 15 agronomic traits. For this, 12 Tongil-type rice cultivars and 15 Japonica-type rice cultivars were transplanted at the experimental farm of the Yeongnam Crop Experiment Station on May 25 in 1987. Jangseongbyeo belonging to Tongil-type had the greatest dissimilarity total among 27 rice cultivars and followed by Chilseongbyeo, Gayabyeo, Hangangchalbyeo, Gihobyeo (Tongil-type) and Sangpoongbyeo (Japonica-type) while cultivars having greatest similarity total were Daecheongbyeo and Yeongsanbyeo and followed by Nagdongbyeo, Sinsunchalbyeo, Gwangmyeongbyeo, Yeongdeogbyeo, Palgongbyeo and Dongjinbyeo that were all belonging to Japonica-type cultivars. CuItivar pairs having the greatest dissimilarity coefficient (50-55%) were combinations of Gayabyeo and Sangpungbyeo, Gihobyeo, or Seonambyeo, combinations of Chilseongbyeo and Jangseongbyeo, Sangpung-byeo, Chucheongbyeo, Gihobyeo or Seonambyeo and combinations of }angseongbyeo and Gwangmyeong-byeo, Sangpungbyeo, Gihobyeo, Daechangbyeo or H waseongbyeo. On the other hand, cultivar pairs having the greatest similarity coefficient (above 85 %) were combinations of Dongjinbyeo and Sinsunchalbyeo, Daecheongbyeo, Somjinbyeo or Yeongsanbyeo, combination of Sangpungbyeo and Gihobyeo, combinations of Yeongdeogbyeo and Yeongsanbyeo or Palgongbyeo, combination of Gwangmyeongbyeo and Yeongdeogbyeo, Combinations of Nagdongbyeo and Gwangmyeongbyeo, Yeongdeogbyeo, Daecheongbyeo, Hwaseongbyeo or Yeongsanbyeo, combinations of Sinsunchalbyeo and Daecheongbyeo or Yeongsanbyeo, combinations of Somjinbyeo and Daecheongbyeo or Yeongsanbyeo and combination of Daecheongbyeo nd Yeongsanbyeo. Two-dimensional ordination analysis method provided the information on cultivar classification between Japonica-type and Tongil-type cultivars except a few cultivars. Only two cultivars, Joongweonbyeo and Seomjinbyeo were located opposit area that implied Tongil-type cultivar Joongweonbyeo was rather close to Japonica-type while Japonica-type cultivar Seomjinbyeo was rather close to Tongil-type, respectively. Sinsunchalbyeo and Daecheongbyeo, on the other hand, were located on the line between two cultivar groups. The method of two-dimensional ordination analysis concluded as feasible approach to classify cultivars Quantitatively for the practical purpose.

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The Effects of Beak Trimming and Crossbreeding-Combinations on the Productive Performance and Stress Response Levels of Korean Native Chickens (한국 토종닭에 있어 부리 다듬기 및 교배조합이 생산능력과 스트레스 반응 정도에 미치는 영향)

  • Ka Bin Shin;Sang-Hyon Oh;See Hwan Sohn
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.213-229
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    • 2023
  • This study aimed to investigate the effects of beak trimming and crossbreeding-combinations on the productive performance and stress response levels of Korean native chickens. The study divided 248 individuals from six crossbreeding-combinations into two groups: one underwent beak trimming, and the other did not. The survival rate, body weight, egg production rate, egg quality, feather damage score, HSP-70 gene expression level, H/L ratio, and intracellular DNA damage rate were measured and analyzed. The results showed that the beak-trimmed group had significantly higher survival rates and hen-housed egg production compared to the non-beak-trimmed group (P<0.05). Feather damage and DNA damage rates were significantly lower in the beak-trimmed group (P<0.05). On the other hand, there were no significant differences between the two groups in adult body weight, hen-day egg production, egg quality, HSP-70 gene expression level, and H/L ratio. Among the crossbreeding-combinations, there were significant differences in survival rate, body weight, feather damage score, egg quality, and DNA damage rate (P<0.05), while egg production rate, HSP-70 gene expression level, and H/L ratio showed no significant differences. There was an interaction between beak trimming and crossbreeding-combinations in some traits. In conclusion, beak trimming in Korean native chickens has a positive impact on productive performance, and in terms of stress response, beak trimming may not act as a stress factor or may even reduce stress after the growing period. Furthermore, there were differences in productive performance and stress response levels among crossbreeding-combinations, but the effects of beak trimming were similar across these combinations.

Bipolar Heterothallism, a Principal Mating System of Cordyceps militaris In Vitro

  • Shrestha Bhushan;Kim Ho Kyung;Sung Gi Ho;Spatafora Joseph W.;Sung Jae Mo
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.9 no.6
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    • pp.440-446
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    • 2004
  • Interest in in vitro study of entomopathogenic fungi, including Cordyceps species, has been increasing due to their valuable bioactive compounds and biocontrol effects. Among Cordyceps species, in vitro stromata of C militaris has been successfully produced and cultivated for industrial purposes. However, genetic study on in vitro stromata formation of C militaris has not been carried out yet. Here, relationship between mating system and perithecial stromata formation of C militaris is reported. Mating system was determined by observing perithecial stromata formation from mono-ascospore cultures and their pair-wise combinations. Certain combinations of mono-ascospore strains produced perithecial club-shaped stromata, whereas other combinations produced either no stromata or only abnormal non-perithecial stromata. Similarly, mono­ascospore cultures without combination produced either no stromata or only abnormal non­perithecial stromata. Despite obvious heterothallism, self-fertility was occasionally observed in few strains of C militaris. These observations indicated that C militaris behaves as a bipolar het­erothallic fungus and requires two mating compatible strains in order to produce regular club­shaped perithecial stromata, a fundamental requirement for its industrial cultivation.

Comparison between reinforced concrete designs based on the ACI 318 and BS 8110 codes

  • Tabsh, Sami W.
    • Structural Engineering and Mechanics
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    • v.48 no.4
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    • pp.467-477
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    • 2013
  • Municipalities in the United Arab Emirates approve reinforced concrete design of building structures to follow either the ACI 318 or the BS 8110 code. Since the requirements of these codes are different from each, there is a need to compare the structural demand in the two codes. The main objective of this study is to compare the design requirements of the ACI 318 code with the BS 8110 code for the flexural, shear and axial compression limit states. The load factors and load combinations in the two codes are also compared. To do so, a large number of cross-sections with different geometries, material properties, and reinforcement ratios are analyzed following the procedures in the two codes. The relevant factored load combinations in the two codes are also investigated for a wide range of live-to-dead load ratios and for various wind-to-dead load ratios. The study showed that the differences between the design capacities in the ACI 318 and BS 8110 codes are minor for flexure, moderate for axial compression, and major for shear. Furthermore, the factored load combinations for dead load, live load and wind in the two codes yield minor-to-moderate differences, depending on the live-to-dead load ratio and intensity of wind.

Development of a Thermal Design Software for the Heat Recovery Steam Generator of Combined Cogeneration Systems (열병합 복합발전시스템용 폐열회수 보일러 열설계 소프트웨어 개발 연구)

  • Kim, T.K.;Oh, S.D.;Kwon, Y.H.;Seo, S.H.;Kim, B.Y.
    • Proceedings of the KSME Conference
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    • 2001.06d
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    • pp.726-731
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    • 2001
  • A thermal design software is developed for the heat recovery steam generator(HRSG) of combined cogeneration systems. The heat transfer is calculated by using the element method to account for the varying thermal properties across the heat transfer elements. The circulation balance is computed for the evaporator to accurately estimate the steam generation rate and to check the proper circulation of the boiler water through the tubes. The software developed can be used to simulate HRSG systems with various combinations of auxiliary burner, wall superheater, superheater, reheater, evaporator, and economizer. Systems with several different combinations of the system components are successfully tested. And it is concluded that the developed software can be used for the design of heat recovery steam generators with various combinations of heat transfer components.

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Risk Prediction Using Genome-Wide Association Studies on Type 2 Diabetes

  • Choi, Sungkyoung;Bae, Sunghwan;Park, Taesung
    • Genomics & Informatics
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    • v.14 no.4
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    • pp.138-148
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    • 2016
  • The success of genome-wide association studies (GWASs) has enabled us to improve risk assessment and provide novel genetic variants for diagnosis, prevention, and treatment. However, most variants discovered by GWASs have been reported to have very small effect sizes on complex human diseases, which has been a big hurdle in building risk prediction models. Recently, many statistical approaches based on penalized regression have been developed to solve the "large p and small n" problem. In this report, we evaluated the performance of several statistical methods for predicting a binary trait: stepwise logistic regression (SLR), least absolute shrinkage and selection operator (LASSO), and Elastic-Net (EN). We first built a prediction model by combining variable selection and prediction methods for type 2 diabetes using Affymetrix Genome-Wide Human SNP Array 5.0 from the Korean Association Resource project. We assessed the risk prediction performance using area under the receiver operating characteristic curve (AUC) for the internal and external validation datasets. In the internal validation, SLR-LASSO and SLR-EN tended to yield more accurate predictions than other combinations. During the external validation, the SLR-SLR and SLR-EN combinations achieved the highest AUC of 0.726. We propose these combinations as a potentially powerful risk prediction model for type 2 diabetes.

Harmony Evaluation for Same-color Coloration of Makeup and Clothing (메이크업과 의복의 동일색상 배색에 따른 조화감 평가)

  • Jeong, Su-Jin
    • Journal of the Korean Society of Clothing and Textiles
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    • v.31 no.5 s.164
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    • pp.801-812
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    • 2007
  • In this research, 128 stimuli of same-color coloration of lipstick and clothing were evaluated to the degree of harmony in the coordination of makeup and clothing color depending on the combination of eyeshadow color, lipstick color, lipstick tone, clothing style, and clothing tone. As to the brown eyeshadow, vivid lipstick and vivid, dull clothing, dull lipstick and light, dull, dark clothing, dark lipstick and dull, dark clothing among the combinations of red lipstick and clothing, vivid lipstick and vivid, light, dark clothing, dull lipstick and light, dull, dark clothing, dark lipstick and dark, clothing among the combinations of orange lipstick and clothing were evaluated as harmonious coloration regardless of the clothing style. As to the violet eyeshadow, vivid lipstick and vivid clothing, dull lipstick and light, dark clothing, dark lipstick and dull, dark clothing among the combinations of red lipstick and clothing, vivid lipstick and vivid, dull clothing, dull lipstick and vivid, light, dull clothing, dark lipstick and vivid, dull, dark clothing among the purple lipstick and clothing were evaluated as harmonious coloration regardless of the clothing style. Thus, the degree of harmony cannot only be different according to the clothing style even in the same coloration, but also the same clothing style can be perceived to be different degree of harmony depending on how the color and tone of clothing and lipstick are coordinate.