• 한국동물학회지
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• 제32권3호
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• pp.290-303
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• 1989

$\beta$-glucosidase, $\beta$-glucuronidase, N-acetyl-$\beta$-glucosaminidase의 성적 성숙과의 연관서을 조사하기 위하여 흰쥐의 복강내로 testosterone과 dibutryjry cyclic AMP 를 투여하여 위이 효소들의 활성도를 측정하였다. 그 결과 겨세한 실험군에서$\beta$-glucosidase와 N-acetyl-$\beta$-glucosaminidase이 활성도도 거세후 7일째에는 유의성있는 감소효과를 나타내었다. testosterone을 7일간 계속 투여한 경우에는 세 효소의 활성도가 모두 유의성있게 증가하였고 dbcAMP 투여군의 경우는 거세 14일째되는 실험군과 비슷하거나 감소하는 경향이 있었다. 미세구조를 관찰한 결과 부정소 상피세포의 유형은 크게 주세포와 기저세포로 나눌 수 있었으며 주세포는 일반적인 원주상피의 형태를 나타냈으며 소낭을 많이 포함하고 있는 narrow cell이 존재하였다. 특히 부정소미에는 다른 상피세포에 비해 전자밀도가 낮은 light cell이 존재했고 기저세포는 부정소 부위마다 비슷한 형태를 가지는 것으로 나타났으며 상피세포 사이에는 이동능력을 가진 halo cell이 존재했다.

• 한국임상수의학회지
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• 제18권2호
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• pp.116-123
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• 2001

This study was undertaken to find out the effect of Korean safflower seed powder on histopathological changes of cadmium toxicity in mice. Fifty BALB/c mice were divided into a control group(A) and four experimental groups(B, C, D, E) : group A received tap water and basal diet, group B received tap water and diet supplemented with 3% Korean safflower seed powder alone, group C received basal diet and 300 $\mu\textrm{g}$/g of cadmium, group D and E received basal diet supplemented with 3% and 10% Korean safflower seed powder and 300$\mu\textrm{g}$/g of cadmium respectively. Cadmium dissolved in tap water was used, and the Korean safflower seed powder were mixed with feed. All mice were dissected on the 56th day. Histopathological changes in liver, kidney, lung, cortical osseous tissue of femoral shaft, bone trabecular of femur, and epiphyseal cartilage plate of femur were observed. Group B showed no significant changes compared with the control group. But group C showed the unclearness of specific cells in liver, the loss of architecture and focal necrosis of hepatocyte, the glomerular swelling, degeneration and necrosis of convoluted tubules, desquamation and vacuolization of the greater part of the renal tubular epithelium, the marked congestion and thickness of the wall of alveolus in lung, slightly thinning of the cortical osseous tissue in femoral shaft, reduction of cancellous bone volume and marked narrowness of bone trabecular, marked thinning of epiphyseal cartilage plate and irregular arrangement of columnar structure of cartilage cells. On the other hand, Korean safflower seed powder-treated group showed a little convalescent changes and maintained their normal architectures in liner, kidney, lung, cortical osseous tissue of femoral shaft, bone trabecular of femur and epiphyseal cartilage plate of femur.

• 한국패류학회지
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• 제4권1호
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• pp.1-16
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• 1988

The authors observed histochemical and ultrastructural characters on the osphradium of Rapana venosa Valenciennes using light microscope, scanning and transmission electron microscpes. The results were as follows:1)The basic structure of osphradium was bipectinated shape, which consisted of a septum situating in the center of osphradium and numerous osphradial leaflets. On the other hand, Epidermis of ospradial leaflets formed the structure of pseudostratified ciliated columnar epithelium which was composed of an epithelial cell layer, a basal cel layer and a neuropile. 2) Ciliated dpithelial cells:A large number of these cells were observed on the lateral and ventral regions but a small number of them were observed on the dorsal region. These cells had cylindrical microvilli, slender mitochondria and serve fibers.3) Supporting cells: These cells had cylindrical microvilli, spongy layer, electron dense granules, mitochondria and nerve fibers4) Four types secretory epothelial cells: Four distinct types of secretory epithelial cells were recognized and were arbitrily designated as Type I, Type II, Type III and Type IV.cell type I: These cells contained electron denwe granules(diameter, 0.94-1.56${\mu}{\textrm}{m}$), well developed Golgi apparatus and rough endoplasmic reticula, cell type II: These cills contained two types of granules of the different electron density. One was high electron density granules which were 0.4-1.0${\mu}{\textrm}{m}$ in diameter, The other was low electron density granules which were 0.75-1.2${\mu}{\textrm}{m}$ in diameter.cell type III:These cells had fibrous secretory materials and exhibited strongly positive reaction with Toluidine blue.cell type IV:A large number of this type of cells were observed on the ventral region of ospgradial leaflets and positively reacted with periodic acid Schiff reagent. 5)Dark cells contained several electron dense cillaty rootlets and unmerous granules but cellular organelles were not observed.6) Four types basal cells: Four distinci types of basal cells were recognized and arbitrarily designated as Type I, Type II, Type III and Type IV.Cell type I(light cell): These cells exhibited low electuon density and contained short smooth endoplasmic reticula, several vacuoles and granules.

• 한국어류학회지
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• 제7권2호
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• pp.140-149
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• 1995

망상어, Ditrema temmincki 소화관의 형태 조직화학적인 특징은 다음과 같이 요약할 수 있다. 체장에 대한 식도에서 항문까지의 소화관 길이의 비 (RLG)는 0.89정도이며, 소화관에는 식도와 담관 입구사이의 팽창부인 위(胃)가 없다. 소화관은 형태 조직화학적인 특징에 의하여 식도, 식도 - 장이행부, 전장, 중장, 후장, 장 - 직장 이행부, 직장, 직장 - 항문 이행부, 항문으로 나눌 수 있다. 점막주름은 직장에서 가장 복잡한 형태를 보이며, 근육층은 식도와 항문에서 발달된 상태를 나타낸다. 점막주름의 상피층은 식도 전방부에서는 입방세포로 구성되며, 나머지 부위 에서는 원주상피로 구성된다. 소화관에는 PAS 반응에 양성을 나타내는 배상세포와 다당류 흡수세포 (Polysaccharid Absorptive Cell)가 관찰되는데, 후자는 다당류 계통의 영양물질의 흡수기능을 가진다. 망상어 소화관에서 다당류 계통의 영양물질은 주로 후장부에서 흡수된다.

• Archives of Plastic Surgery
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• 제38권3호
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• pp.251-256
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• 2011

Purpose: A nasolabial cyst is a rare non-odontogenic, soft-tissue, developmental cyst arising anywhere on the face inferior to the nasoalar region. It is thought to arise from either epithelial remnants trapped along the lines of fusion during the development of face or the remnants of the developing nasolacrimal duct. This study examines various features of nasolabial cysts with bony involvement to provide a basis for correct diagnosis and treatment. Methods: Eight cases of nasolabial cyst treated in Soonchunhyang Hospital between March 2002 and July 2010 were examined in terms of their clinical features and radiological and histological findings. Seven patients underwent surgical excision of the cyst via an intraoral, sublabial approach. One underwent incision and drainage. Results: Our eight patients were seven women and one man. The most frequent symptoms and signs were facial deformity and swelling of the nasolabial fold. Computed tomography (CT) showed a well-circumscribed cystic mass lateral to the pyriform aperture. Seven cases had erosive lesions on CT, and the intraoperative findings were consistent with a nasolabial cyst with a bony defect. Typical histopathological findings showed that these cysts were most frequently lined with respiratory epithelium with ciliated columnar cells and cuboid cells. No patient developed complications or recurrences. Conclusion: A nasolabial cyst is often unrecognized or confused with other intranasal masses, including fissural and odontogenic cysts, midface infections, or swelling in the nasolabial area. Therefore, a careful clinical and radiological evaluation should be preformed when considering the differential diagnosis. We present eight patients with nasolabial cysts treated via a gingivobuccal approach with excellent functional and cosmetic results.

• 한국임상수의학회지
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• 제22권4호
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• pp.401-403
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• 2005

동물원에서 사육 중이던 7세령 암컷 일본원숭이가 짝짓기 과정에서 피부에 외상을 입은 후 치료 도중 폐사하였다. 부검시 간에서 $1.3\times1.2\times1.0cm$ 크기를 비롯한 다양한 크기의 다발성 낭포가 관찰되었으며 낭포내에 점액성 액체가 저류되어 있었다. 현미경 소견상 낭포는 담관 상피세포로 덮혀 있었고 입방상피에서 원주상피까지 다양하였으며 대부분 단층이었으나 일부는 여러층으로 덮혀 있었다. 악성도나 다른 장기로의 전이 소견은 찾을 수 없었다. 본 증례는 일본 원숭이에서 발견된 첫 번째 담관 낭샘종 증례이다.

• 대한수의학회지
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• 제36권1호
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• pp.209-219
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• 1996

This study examined the effect of alcohol(AL) and/or paraquat(PQ) on serum TSH, thyroid hormones and enzyme activities, and the protective effect of selenium(SE) againse alcohol and/or paraquat-induced thyroid toxicity in guinea pigs. The experomental group consisted of control, 15% alcohol(AL), 4ppm sodium selentite(SE), 200ppm paraquat(PQ), AL+PQ, AL+SE, PQ+SE and AL+PQ+SE mixed in drinking water-fed guinea pigs for 4 weeks. The morphological changes of thyroid gland were studies on paraffin-embedded sections stained with H-E stain. Body weight losses, high serum concentration in TSH and cholesterol, and low values on triiodothyronine($T_3$), thyrozine($T_4$), free $T_4$ and alkaline phosophatase(ALP) were produced in the groups fed AL and/or PQ. We also noted that AL+PQ-fed group was marked increase in serum TSH. In AL or AL+PQ-fed groups when cpmpared to control group had increased the ratio of thyroid weight to body weight(ratio Twt/Bwt), whereas the ratio Twt/Bwt was decresed in SE or PQ-fed groups. However, the serum TSH, $T_3$,$T_4$ free $T_4$ and cholesterol values, and the ratio Twt/Bwt were reversed in groups given the combination of SE, compared with AL and/or Pq-fed groups, also ALP values were reversed in groups given the combination of SE, compared with AL or AL+PQ-fed groups. In microscope, morphological changes showed a remarkable between the AL or PQ-fed group and controls. In AL+PQ+SE-fed guinca pig, follicular colloid is high density in thyroid follicle and increased in connective tissue around the thyroid cells, and thyroidal epithelia were composed of cuboidal or columnar epithelium. The indicated that the morphological changes of thyroid were direct action in the thyroid cell. The results of this study confirmed that the toxic effect of AL or PQ on thyroid occur independently of changes in liver function, and that SE confers marked protection against AL or PQ-induced thyroid toxicity.

• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.301-309
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• 2014

Nesfatin-1, an anorexic nucleobindin-2 (NUCB2)-derived hypothalamic peptide, controls appetite and energy metabolism. Recent studies show that nesfatin-1/NUCB2 is expressed not only in the brain but also in gastric and adipose tissues. Thus, we investigated the distributions of nesfatin-1/NUCB2 in various tissues of male and female mice by real-time PCR, western blotting, and immunohistochemical staining. Real-time PCR analyses showed that NUCB2 mRNA was predominantly expressed in the pituitary and at lower levels in the hypothalamus, spleen, thymus, heart, liver, and muscle of both male and female mice. Expression was much higher in reproductive organs, such as the testis, epididymis, ovary, and uterus, than in the hypothalamus. Western blot analysis of the nesfatin-1 protein level showed similar results to the real-time PCR analyses in both male and female mice. These results suggest that nesfatin-1/NUCB2 have widespread physiological effects in endocrine and non-endocrine organs. In addition, immunohistochemical staining revealed that nesfatin-1 was localized in interstitial cells, including Leydig cells and in the columnar epithelium of the epididymis. Nesfatin-1 was also expressed in theca cells and interstitial cells in the ovary and in epithelial cells of the endometrium and uterine glands in the uterus. These results suggest that nesfatin-1 is a novel potent regulator of steroidogenesis and gonadal function in male and female reproductive organs. Further studies are required to elucidate the functions of nesfatin-1 in various organs of male and female mice.

• 대한수의학회지
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• 제49권1호
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• pp.67-71
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• 2009

A 10-year old female Yorkshire terrier with nasal discharge and swelling was referred to the local animal hospital. Abnormal mass of right nasal cavity was detected in physical examination and radiography. According to the radiographs of the head, there was an evidence of bony destruction in right nose. Oronasal fistula was detected in right maxillary canine teeth. After surgical excision, the sample of nasal mass was refereed to Pathology Department of Veterinary Medicine in Jeju National University. Grossly, the enlarged mass was soft and 3 ${\times}$ 3 cm in size. Histopathologically, the neoplastic mass was composed of tubular to tubulopapillary structures which were lined by single to 6~7 layers of cuboidal to ciliated columnar cells. These neoplastic cells showed invasive tendency to adjacent normal parenchyma. They had uniform, round to oval nuclei, cytoplasm with small vacuoles and indistinct cellular margin. The number of mitotic figures was varied in different areas, ranged from 0 to 4 per high power field. Necrotic foci and infiltration of inflammatory cells including neutrophils, lymphocytes, and plasma cells also presented in the mass. Immunohistochemically, the neoplastic cells demonstrated strong positive reaction for cytokeratin (CK) 18 but were negative for CK 7 and 8. Based on the gross, histopathology and immunohistochemistry, this mass was diagnosed as nasal adenocarcinoma originated from respiratory epithelium.

• 대한방사선기술학회지:방사선기술과학
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• 제3권1호
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• pp.95-105
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• 1980

흰불나방(Hyphantria cunea Drury)의 각(各) 변태단계(變態段階)에 따라 $1.75{\sim}7krad$의 Cobalt-60 $\gamma$선(線)을 전신조사(全身照射)하여 $\gamma$선조사(線照射)에 의(依)한 중장세포(中腸細胞)의 조직학적(組織學的) 변화(變化)를 광학현미경(光學顯微鏡)으로 정상군(正常群)과 비교(比較) 관찰(觀察)하였던 바, 다음과 같은 결과(結果)를 얻었다. 1. 중장(中腸)의 상피세포(上皮細胞)는 원주세포(圓柱細胞), 배상세포(杯狀細胞), 신생세포(新生細胞)로 구성(構成)되어 있었다. 2. 조사(照射)의 영향(影響)은 조사(照射)된 선량(線量)과 변태시기(變態時期)에 따라 변화(變化)되었다. 3. 조직학적(組織學的) 변화(變化)의 정도(程度)는 조사선량(照射線量)이 많을수록 증대(增大)되었다. 4. 방사선(放射線)의 감수성(感受性)은 종령유충(終齡幼蟲), 용8일(日)에서 가장 높았다.