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Investigation of Physicochemical Properties of Safflower Sprouts Grown Different Wavelengths of Visible Light and Treated with Different Drying Processes (다른 광원 조사로 재배된 홍화새싹의 건조 방법별 이화학적 특성)

  • Choi, Mi-Kyeong;Chang, Moon-Sik;Eom, Seok-Hyun;Jung, Uk-Sun;Kang, Myung-Hwa
    • Korean journal of food and cookery science
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    • v.29 no.6
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    • pp.717-724
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    • 2013
  • Physicochemical properties of safflower sprouts grown in there different lights red, blue, and white; control exposed to limited light and subjested to different drying processes were investigated. Moisture contents of safflower sprouts grown different lights and treated with hot dry air were as follows: 9.38%(white light), 5.70%(blue light), 12.21%(red light), and 7.84%(control; no light). The moisture contents of freeze-dried safflower sprouts were as follow as: 9.95%(white light), 11.30%(blue light), 11.25%(red light), and 10.45%(control). Crude ash contents of the hot air-dried sprouts were as follows: 4.82%(white light), 12.22%(blue light), 4.33%(red light), and 6.30%(control). The crude ash contents of freeze-dried safflower sprouts were as follow as: 4.75%(white light), 4.44%(blue light), 4.00%(red light), and 3.65% (control). Crude protein contents of hot air-dried safflower sprouts were as follows: 16.52%(white light) 15.40%(blue light), 14.55%(red light), and 15.46%(control). The crude ash contents of freeze-dried safflower sprouts were as follows: 25.19%(white light), 15.72%(blue light), 25.53%(red light), and 20.13%(control). Chlorophyll contents of hot air-dried and freeze-dried safflower sprouts were higher than those of only freeze-dried sprouts. b-values of freeze-dried sprouts were lower than those of sprouts dried by other dried methods. The contents of minerals Ca, Mg, Na, P and K in all the sprout samples remains unchanged regardless of the drying methods.

Herpes Simplex Virus Thymidine Kinase Gene Therapy Delivered by Retroviral or Adenoviral Vector in Mouse Model of Lewis Lung Carcinoma (Lewis 폐암 마우스 모델에서 Retroviral Vector나 Adenoviral Vector로 이입된 Herpes Simplex Virus Thymidine Kinase 유전자치료)

  • Kwon, Hee-Chung;Jeong, Jae-Min;Kim, Jung-Hyeon;Ham, Yong-Ho;Seo, Ji-Sook;Lee, Ki-Ho;Kim, Chang-Min;Lee, Han-Soo;Lee, Choon-Taek
    • Tuberculosis and Respiratory Diseases
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    • v.49 no.3
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    • pp.298-309
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    • 2000
  • Background : The antitumor effects of herpes simplex virus thymidine kinase (HSV-tk) and ganciclovir (GCV) strategies for cancer gene therapy have a the following advantages : 1) a direct cytotoxicity to HSV-tk modified cancer cells by GCV 2) a cell death by the local transfer of toxic metabolites from the HSV-tk modified cells to nearby unmodified tumor cells (bystander effect), and 3) in vivo bystander effect such as antitumor-immunity. Retroviral and adenoviral sequences can silence transgene expression in cells and mice. In this study, we investigated the above described advantages of HSV-tk/GCV strategy in Lewis lung cell and mouse lung cancer model using retroviral vector and adenoviral vector. Also, we observed whether the expression of a silenced gene can be reactivated by treating cells with butyrate. Methods : Retrovirus-HSV-tk and adenovirus-HSV-tk vectors were used for the transduction of Lewis lung carcinoma (LLC) cells. The change of HSV-tk expression by butyrate was measured by Western blol The antitumor activities containing bystander effect were observed in vivo (by MTT assay) and in vivo tumor models of various combinations of LLC and LLC-tk. Results : 1. Butyrate induced the enhancement of HSV-tk expression from adenovirally transduced cells but not from retrovirally transduced cells. 2. Both retrovirus-HSV-tk and adenovirus-HSV-tk vectors with GCV treatment were effective for killing of tumor cell in vitro and suppression of LLC tumorigenicity. Bystander effect was responsible for killing of mixture of LLC-tk and LLC in vitro and in vivo-tumorigenicity model. Conclusion : Butyrate could augment adenovirus-mediated HSV -tk gene expression. Cancer gene therapy with HSV-tk suicide gene by retroviral and adenoviral vector seems to be an effective approach for lung cancer therapy.

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Investigating Research on the Degree of Frequency and Importance of Tasks for Framing the EMTs Occupational Description (응급구조사 직무기술서 작성에 따른 일의 요소별 빈도 및 중요도 조사 연구)

  • Kim, Tae Min;Kim, Hyo Sik;Yoou, Soon Kyu
    • The Korean Journal of Emergency Medical Services
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    • v.5 no.1
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    • pp.199-212
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    • 2001
  • This research, aiming at analyzing the Emergency Medical Technicians' duties prior to establishing a baseline for question development in the national exams to qualify for the EMTs, investigated the importance and frequency per unit specified in the description with the EMTs to be subjects working on the task spot by means of questionnaires. The EMT duties were classified into 9 items like 1) the notification and response, 2) the scene size-up, 3) the patient assessment, 4) the emergency care, 5) the patient transport, 6) the duties at hospital clinics, 7) the operational management, 8) the receiving and responding to a call, 9) the self-development, and put the functional tasks into 52 items, describing the task elements into 177 items, and then questioned 112 EMTs working on the spot from July 21 through August 30, 2000, which showed the following results. 1) The distribution of subjects' career showed the highest rate with 33.9% for those who careered "less than a year", only 13.4% for those with more than 4 years and the highest rate with 43.8% for those who aged at 20-25. And 70.5% of all those who were questioned was the junior college graduates, 58.9% for those working at fire station and 29.5% working at hospital clinics. 2) Looking at the distribution of frequency and importance for each task element, questioning 'the patients main symptoms', 'accidental type', 'place of the patients identification' showed the highest rate in both frequency and importance in the field of "notification and response". 3) In the "scene size-up", identifying the patients showed the highest rate of frequency and importance, compared to other field of tasks, among which "identifying the patients' state" showed the highest rate of frequency (2.66) and importance (2.81). 4) In the "patient assessment", "identifying the patients" showed the high rate of frequency and importance in most elements of task, especially the importance showed the highest rate with 2.83 for the task of airway management and the cervical immobilization during "the primary assessment", and the frequency showed the highest rate for questioning the past case of the task of grasping the patients' history. 5) In the "emergency care", "the management of the heart attacked patients" and "the advanced cardiac life support" showed a high rate in the importance, whereas the frequency showed a very low rate. The high rate of frequency during the emergent task was the management of "musculoskeletal system injury patients" and of "the gastro-intestinal and urinary genital system injury patient support." In care of the patients with heart attack, the management of the airway showed the highest rate with 2.95, whereas the management of alcoholic abused patients and of dying patients, showed comparatively lower rate of assessment in the importance. The frequency of tasks showed the highest rate with 2.69 in the control of bleeding and the lowest with 0.47 in the management of abnormal delivery of child. 6) As to the patient transport, "the emergency transport" showed the highest rate with 2.74 and the unemergent transport with 2.55 in the importance, and the task importance at hospital showed the highest rate with 2.89 in managing the cardio-pulmonary resuscitation and with 2.60 in identifying the patient state. 7) Of all the tasks related with "operational task", the high importance was to "educate for the management of the first responder", but the frequency of tasks mostly showed a low rate and "receiving and responding to a call" showed relatively a high rate of importance and frequency. And related with "the self-development", "the health care management" and "the stress control" on working spot showed a high rate, but the frequency mostly showed a low rate.

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Pond Vegetation Assessment of Golf Courses in the Capital Region of Korea (수도권 지역 골프장의 연못식생 평가)

  • Kim Chang-Hwan;Ahn Deug-Soo
    • Journal of Environmental Science International
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    • v.14 no.3
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    • pp.279-288
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    • 2005
  • The vascular plant at Korea CC was researched in 95 taxa and Korea CC had the highest number of vascular plants among the ponds of 9 golf courses in the capital region. The number of vascular plants at Eunhwasam CC was 21 taxa which followed as the second most. Most of the vascular plants surveyed consist of a mon-ocotyledon with 1-2 years of life and a dicotyledon as perennial. The ratio between an introduced species of pond vegetation and planting species from Jisan CC is $52.3\%$, which is the highest. The lowest ratio of those is $21.1\%$, which is from Pine Creek Cc. Pine Creek CC has 25 plant communities in total, with 16 semi-natural and natural communities and 9 planting or introduced communities. Pine Creek CC has the most varied plant communities among 9 golf clubs. There are plant communities of semi-natural and natural vegetation from all ponds, and there are 12 aquatic plant communities at disturbance or planting vegetation. We found out that Pine creek CC has more natural communities than disturbance or planting communities compared to other golf clubs, and there is not any natural vegetation at Pristine Valley CC. According to value grade from assessment indicator, the natural aspect of Seseoul CC and Eunhwasam CC are the lowest among 9 golf clubs. Taeyoung CC, Eunhwasam CC, Jisan CC, Seseoul CC are superior in made vegetation among the group in terms of euclidean similarity coefficient on natural aspect and are classified as one group. Korea CC and Pristine Valley CC have distinguished semi-natural vegetation and Midas Valley CC and Pine Creek CC, which have a relatively high natural aspect, are classified as one group. Blue Heron CC is separated from other groups into its own group because it has a high value of scenery in particular. In conclusion, there is a correlation between the natural aspect of a pond and size of vegetation.

Analysis of Consciousness and Model on Land for the Another use After Quarrying (채석장의 부지 활용에 대한 의식 및 모델 분석)

  • Park, Jae Hyeon
    • Journal of Korean Society of Forest Science
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    • v.101 no.3
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    • pp.387-394
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    • 2012
  • The study was conducted to develop an effective forest resources use models for an alternate use of abandoned quarry by an attitude survey. According to the result of survey, a pessimistic view due to dust, noise pollution, and forest damage was 5% higher than an affirmative view by economic benefits from the development of quarry. The 42% of the respondents preferred the alternate use of abandoned quarry and the 25% of the respondents wanted an art and cultural space. The optimum size of alternate use was 5-10 ha (43%) with the requirement of nearby residents (32%). According to the SWOT analysis for abandoned quarry, the strength factors were an effective use of land, the content development of modern industrial inheritance + cultural and art fusion, attraction for nearby city and visitors, a harmony of beauty landscape and clean environment, and a sustainable increase of domestic and foreign visitors with the 5-day-work week. The opportunity factors were the improvement of traffic networks through KTX and local highway, the creation of the new growth engines with the establishment of artistic creation belts, the providing of unique cultural and art space through grafting of tour and education, the creation of local income through stone processed goods, and the vitalization of local development through eco-city. The weakness factors were a psychological remoteness and backwardness, and the weakness of staying tour infra. The threat factors were a poor financial support for sustainable development in nearby quarry and a modify of legal and institutional system for the alternated use of abandoned quarry. The developed restoration models for the alternate use in abandoned quarry are classified to a sculpture park, a waterfall and lake park, a rock-climbing, a sports park + forest park, a native botanical garden, a culture and art park, a complex park, a water storage site, a water storage site to extinguish forest fire, a geriatric hospital, an agricultural facility, and a school site types etc. The results suggest that the alternate use in the abandoned soil and stone quarry is needed to establish facility use models with consideration of user's preference.

A Study on the Current Status and Satisfaction of the Art, Music, and Physical Education in Local Child Care Center (지역아동센터의 예체능교육에 대한 현황과 만족도에 관한 조사 연구)

  • Bae, Na-Rae
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.10
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    • pp.163-169
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    • 2017
  • The purpose of this study is to present the basic data needed to improve the arts, music, and physical education provided by local child care centers based on an investigation of the current status of and satisfaction with the education. The subjects of this study were 17 local child care centers in Gyeonggi-do, South Korea, and the situation of the arts, music, and physical education programs operated from 2014 to 2016 were examined. In addition, 419 children who received the education were surveyed to evaluate their level of satisfaction. The results of this study are as follows. As regards the status of the arts education from 2014 to 2016, it was observed that three of the 17 local child care centers did not have any arts, music or physical education at all, while six of them continuously implemented all three of these programs during this period of time. Two and six of the 17 institutes had arts, music, and physical education programs for two years and one year, respectively. All of the teachers who ran the arts and music education programs of the 17 institutes were arts and music majors who were certified teachers of the liberal arts. However, the physical education programs were run as volunteer activities by college students majoring in physical education. The survey on the level of satisfaction of the children who participated in the arts, music, and physical education programs showed that they were helpful for the overall life experience of the children and that they were more helpful for the boys than for the girls. The level of satisfaction with the education was high for most of the students who participated in the programs, however the boys were more satisfied than the girls. When asked whether they would participate in the arts, music, and physical education programs again, most of the respondents answered that they would do so. The boys were more likely to participate again than the girls. Based on this study, in order to enhance the creativity and personality education of the children using the local child care centers, higher quality education is needed. Arts and music education can be used to help children to learn to communicate smoothly with their friends. In addition, it seems to be necessary to enhance the education by setting goals that are suitable for its purpose, in order to provide creative arts and music education that contributes to the physical health and emotional stability of the children.

A Survey of Korean Housewives' Perception on the Commercial Korean Basic Side Dishes in Busan Area (시판 밑반찬에 대한 부산지역 주부의 인식조사)

  • Lyu, Eun-Soon;Lee, Dong-Sun;Chung, Sun-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.4
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    • pp.440-447
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    • 2006
  • The perception of Korean housewives was investigated on commercial Korean basic side dishes. The questionnaires were distributed to 464 housewives in Busan area. According to the survey, the housewives preferred discount stores (44.1%) to purchase basic side dishes. The reasons for purchasing them were convenience (54.6%) and readiness in small amount (23.1%). The housewives had high perception scores on saying cooking time (4.90/5.00), the possibility of purchasing when they need (3.93/5.00), and convenient use (3.85/5.00). However, they doubted the propriety of making use of chemical seasoning (2.15/5.00), the original place of raw food materials (2.21/5.00), and shelf life control (2.44/5.00). The satisfaction scores on the commercial basic side dishes were 3.06/5.00 in braised perilla leaves, 2.98/5.00 in braised soybean, 2.96/5.00 in pickled garlic, and 2.96/5.00 in stir-fried/muchim dried fish. The priorities on the improvement demand for the basic side dishes were appeared in order of the sanitary preparation, the consistent tastes, the proper amounts of chemical seasonings, and sanitary distribution systems.

Damage Analysis of Korean White Pine Stands in which the Black-tipped Sawfly was Chemically Controlled (잣나무넓적잎벌 방제림분(防除林分)에 대(對)한 잣나무 피해해석(被害解析))

  • Chung, Sang Bae;Kim, Chul Su
    • Journal of Korean Society of Forest Science
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    • v.87 no.3
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    • pp.328-333
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    • 1998
  • To obtain basic information for establishing a pest control strategy for insect pest management system, changes in the population densities of the black-tipped sawfly(Acantholyda posticalis posticalis Matsumura) and damage patterns in tree growth were investigated in national forests in Hoigok-ri, Kapyung-gun, Kyunggi-do, where the pest control measures were taken. The results obtained were as follows ; 1. The larval density in the soil of the forests where the insecticides were applied have been kept below economic threshold for about 7 years. The density was the highest in the middle of slopes and similar to the level of the early stage of the insect outbreak. 2. After the pest control by insecticides, reduction in tree height and diameter growth lasted for 2-3 years in trees defoliated by over 70%. 3. The diameter growth of the trees damaged by black-tipped sawfly recovered faster in upper stem than in the lower. 4. volume growth of the trees defoliated over 70% by the insect decreased for three to four years. The volume loss of trees defoliated by 70% and 90% was 19.6% and 54.0%, respectively. 5. Maintaining the rate of defoliation below 50%, which is the economic threshold, by chemical control measures had an effect of reducing the tree volume loss by $40m^2/ha$ as compared with a stand defoliated by 90%.

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Studies on Restoration of Forest-Floor Vegetation Devastated by Recreational Trampling (I) -Seeding, Fertilizing and Soil Surface Treatment Effect on Restoration of Forest-Floor Vegetation- (답압(踏壓)으로 훼손(毁損)된 임간나지(林間裸地)의 임상식생복원(林床植生復元)에 관한 연구(硏究)(I) -임상식생복원(林床植生復元)에 미치는 파종(播種), 시비(施肥) 및 표토처리효과 (表土處理效果)-)

  • Oh, Koo Kyoon;Woo, Bo Myeong
    • Journal of Korean Society of Forest Science
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    • v.81 no.1
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    • pp.53-65
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    • 1992
  • For elucidating effective methods of restoration of forest recreational sites where management goals are maintaining naturalness and conserving natural ecosystem, seeding, fertilization and soil surface treatment were used for four years at the devastated forest-floor. For restoration of forest-floor vegetation, factorial experiment was used with a split plot design(main plot : fertilization, subplot : soil surface${\times}$seeding) and a randomized complete block design (fertilization${\times}$seeding) at the Kwanaksan Aboretum, Anyang, Kyonggido. Results were summarized as follows : Soil surface softening with tipping and ripping and straw-mat mulching (70% coverage) treatment was effective on germination, survival and growth of seeded vegetation at devastated forest-floor. Especially, straw-mat mulching treatment was effective on soil surface stabilization and seedling's survival at eroded soil surface, while complete soil surface softening treatment was effective on germination, survival and early growth of tree species of late-successional series. Introducing seeds of native species of pioneer or early-successional series, with good growth capability in barren soil was effective on rapid restoration in devastated forest-floor with its soil surface previously compacted and its surviving seeds washed away. When the seeding and straw-mat mulching after partial soil surface softening with tipping and ripping treatment were employed, it took about three years to restore the devastated forest-floor where surface erosion had been undertaken for an extended period of time and where naturally surviving seeds of native species had been washed away. Softening treatment of soil surface was effective for about two years, and seeding and soil surface treatment increased number of seedlings and improved soil surface environment through fixing of movement of the fallen leaves. Fertilizing effect was not oberserved, mainly due to seeding exposure and poor physical condition including soil surface erosion, low soil water potential and drought, etc, at the field experimental site. However, application of nitrogen and phosphate fertilizers was effective on seedling survival of the species in late-successional series, while lime application adversely affected the seedling survival.

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Effects of Auxin and Fog Treatments on the Green-Wood Cutting of the Mature Trees in Prunus yedoensis (왕벚나무 성숙목의 녹지삽목에서 Auxin 및 Fog 처리 효과)

  • Kim, Chang-Soo;Kim, Zin-Suh
    • Journal of Korean Society of Forest Science
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    • v.96 no.6
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    • pp.676-683
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    • 2007
  • In an attempt to develop an efficient method for the propagation of mature Prunus yedoensis (45 to 55 years old), green wood cuttings from normal branch and sprouts branch were treated with three different kinds of auxin (Rootone < 1-naphthylacetamide 0.4% >, IBA 100 ppm, and control and two different kinds of fog systems (0.9 L/min. and 0.54 L/min.). The Rootone treatment showed higher values in the percentage of rootings (PR) and the mean number of roots per cutting (NR) than the IBA treatment in the early stage. However, in the late stage, the values of PR and NR in the Rootone treatment become lower than those in the IBA 100 ppm treatment. On the other hand, root development ceased 62 days after taking cuttings for all of the treatments. The IBA 100 ppm treatment showed the best performance in root development (PR= 89.5%, NR = 6.5, LR=6.4 cm). The values of PR (76.5%) and NR (6.4) in the 0.9 L/min. of fog treatment was higher than those (PR = 71.7% and NR = 5.4) in the 0.54 L/min. of fog treatment. The cuttings from sprouts (PR: 74.8%, NR: 5.9, LR: 5.7 cm) showed slightly better performance in rooting rate that the cuttings from shoots (PR : 73.3%, NR: 5.9, LR: 5.4 cm). Statistically significant interactions were presented among most of the different combinations of three factors (auxin treatments, fog treatments, and types of cuttings). The PR showed the highest value of 98.0% in the combination of cuttings of shoots+IBA 100 ppm+0.54 L/min. fog treatments. In case of NR, the cuttings from normal branch showed a higher value than the cuttings from sprouts branch under the fog treatment of 0.9 L/min., while this tendency was reversed under the fog treatment of 0.54 L/min.. The perigon development of roots, which reflects the number and the direction of roots, was best in the IBA treatment (85.6%).