• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2000.05a
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• pp.64-75
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• 2000

During the last three decades considerable advances has been made in goat embryo production and transfer technology. The Korean native black goat is the most useful domestic ruminant in this country for biological investigation and application because it has a lot of merits such as relatively short generation period (1 vs 2 year for a cow), easy of handling, well adaptation, high fertility, convenient and inexpensive. This article covers the methods of superovulation, estrus synchronization, embryo collection and transfer techniques, pregnancy diagnosis and subsequent pregnancy and kidding rates for the production of transgenic Korean native black goats. More than one hundred goat kids have been produced as a result of our transgenic goat project via microinjection of foreign gene into pronuclei, in vitro culture, transfer of various stages of fresh and frozen-thawed microinjected embryos into oviducts or uteri of recipient does. We have got two transgenic goats carrying a transgene targeting the expression of recombinant human granulocyte colony stimulating factor (hG-CSF) to the mammary gland so far. Since collection and transfer of embryos in this species is usually accomplished by laparotomy, exteriorization of the reproductive tract for surgical embryo collection leads to the formation of post-operative adhesions. Nonsurgical or laparoscopic technique to reduce adhesions from repeated surgeries has great advantages in improving embryo production and transfer especially from valuable donors. We will discuss this later.

• Proceedings of the Microbiological Society of Korea Conference
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• 2002.10a
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• pp.90-93
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• 2002

The revolution in molecular biology has given us greatly increased ability to obtain and to modify biological resources and to use them for the benefit of all humankind. The sequencing and the associated analysis of gene functions for a growing number of genomes will have an unprecedented effect on the uses of biological resources and the need for access to them. To investigate the diversity of microbial community in swamp, molecular systematic methods were applied. By amplified rDNA restriction analysis (ARDRA) and rDNA partial sequence analysis, $75\%$ of the isolates were known species. In case of uncultured analysis, almost all the selected clones were new species candidate. Especially archea and uncultured bacterial analyses, all clones were new taxon candidates. As for the eukaryotic diversity, several yeast form cultures were isolated from various samples of swamp. Among them, about $60\%$ of the isolates were easily identified. In case of a new species candidate, most strain were included in hymenomycetal yeasts.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.9 no.9
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• pp.3396-3411
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• 2015

Sensors used in Wireless Sensor Networks (WSN) have mostly limited capacity which affects the performance of their applications. One of the data-gathering methods is to use mobile sinks to visit these sensors so that they can save their limited battery energies from forwarding data packages to static sinks. The main disadvantage of employing mobile sinks is the delay of data collection due to relative low speed of mobile sinks. Since sensors have very limited memory capacities, whenever a mobile sink is too late to visit a sensor, that sensor's memory would be full, which is called a 'memory overflow', and thus, needs to be purged, which causes loss of collected data. In this work, a method is proposed to generate mobile sink tours, such that the number of overflows and the amount of lost data are minimized. Moreover, the proposed method does not need either the sensor locations or sensor memory status in advance. Hence, the overhead stemmed from the information exchange of these requirements are avoided. The proposed method is compared with a previously published heuristic. The simulation experiment results show the success of the proposed method over the rival heuristic with respect to the considered metrics under various parameters.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2000.06a
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• pp.64-75
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• 2000

During the last three decades considerable advances has been made in goat embryo production and transfer technology. The Korean native black goat is the most useful domestic ruminant in this country for biological investigation and application because it has a lot of merits such as relatively short generation period(1 vs 2 year for a cow), easy of handling, well adaptation, high fertility, convenient and inexpensive. This article covers the methods of superovulation, estrus synchronization, embryo collection and transfer techniques, pregnancy diagnosis and subsequent pregnancy and kidding rates for the production of transgenic Korean native black goats. More than one hundred goat kids have been produced as a result of our transgenic goat project via microinjection of foreign gene into pronuclei, in vitro culture, transfer of various stages of fresh and frozen-thawed microinjected embryos into oviducts or uteri of recipient does. We have got two transgenic goats carrying a transgene targeting the expression of recombinant human granulocyte colony stimulating factor(hG-CSF) to the mammary gland so far. Since collection and transfer of embryos in this species is usually accomplished by laparotomy, exteriorization of the reproductive tract for surgical embryo collection leads to the formation of post-operative adhesions. Nonsurgical or laparoscopic technique to reduce adhesions from repeated surgeries has great advantages in improving embryo production and transfer especially from valuable donors. We will discuss this later.

• Journal of Korean Public Health Nursing
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• v.19 no.2
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• pp.217-228
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• 2005

Purpose: The purpose of this study is to understand nursing students' experiences during clinical practice at a public health center. Method: This research used narrative inquiry far data collection. From April 2005 to June 2006, data collection was conducted by open-ended interview, questionnaire and close observation. The participants, who were student nurses, were willing to take part in this study. Results: On the basis of these data, the experiences of clinical practice at public health center were: 1) when the student nurses begin clinical practice at public health centers for the first time, most of the students feel fearful, nervous and stressed. They also mentioned having a hard time being polite to clients and the staff. 2) The students had new experiences at the health public center compared with clinical practice. Especially, the student nurses who were determined to be good nurses were doing home visiting care service. Not only did they have the opportunity to confirm their identity as nurses, but also the students change their career course from clinical nursing to public health nursing. 4) They reflected on themselves after home visiting care service. Conclusion: On the basis of these findings, the following recommendations are made. 1) Data collection and analysis are needed, net only through the narrative method, but also through other various qualitative methods. 2) Comparative study is necessary to enhance clinical experiences through the analysis of the interfering factors and the original experiences.

• The Korean Journal of Emergency Medical Services
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• v.23 no.2
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• pp.75-86
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• 2019

Purpose: It is crucial that 119 emergency medical technician (EMT) have adequate knowledge regarding forensic science. This study aimed to assess the forensic knowledge of EMTs in the Republic of Korea. Methods: This study was conducted on EMTs of five fire stations in D metropolitan city between August 6 and August 13, 2018. A questionnaire consisting of items on crime-related incident recognition (i.e., mechanical asphyxiation, trauma, sex crimes, and child abuse), forensic evidence management (i.e., evidence collection, preservation, and recording) was administered to the participants. Finally, 119 questionnaires were analyzed. Results: The ratio of correct answers for crime-related incidents recognition was 55.82%, which was lower than that for forensic evidence management(84.5%). In particular, the scores for the type of neck compression(16.5%) and wound assessment(44.0%) were low. The percentage of correct answers for forensic evidence management was 78.4% for evidence collection, 84.4% for evidence preservation, and 90.6% for evidence recording. Previous forensic education experience was not a significant variable. Conclusion: Specific and practical forensic science education on perception of crime-related incident, including asphyxia and wound identification, and forensic evidence collection is needed.

• Tunnel and Underground Space
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• v.28 no.6
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• pp.547-554
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• 2018

Using crowdsourced sensor data collection technique, it is possible to collect high-density ground vibration data which is difficult to obtain by conventional methods. In this study, we have developed a crowdsourced ground vibration data collection system using MEMS sensors mounted on small electronic devices including smartphones, and implemented client and server based on the proposed infrastructure system design. The system is designed to gather vibration data quickly through Android-based smartphones or fixed devices based on Android Things, minimizing the usage of resource like power usage and data transmission traffic of the hardware.

• International Journal of Oral Biology
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• v.46 no.1
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• pp.1-6
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• 2021

Since the outbreak of coronavirus disease 2019 (COVID-2019), the infection has spread worldwide due to the highly contagious nature of severe acute syndrome coronavirus (SARS-CoV-2). To manage SARS-CoV-2, the development of diagnostic assays that can quickly and accurately identify the disease in patients is necessary. Currently, nucleic acid-based testing and serology-based testing are two widely used approaches. Of these, nucleic acid-based testing with quantitative reverse transcription-PCR (RT-qPCR) using nasopharyngeal (NP) and/or oropharyngeal (OP) swabs is considered to be the gold standard. Recently, the use of saliva samples has been considered as an alternative method of sample collection. Compared to the NP and OP swab methods, saliva specimens have several advantages. Saliva specimens are easier to collect. Self-collection of saliva specimens can reduce the risk of infection to healthcare providers and reduce sample collection time and cost. Until recently, the sensitivity and accuracy of the data obtained using saliva specimens for SARS-CoV-2 detection was controversial. However, recent clinical research has found that sensitive and reliable data can be obtained from saliva specimens using RT-qPCR, with approximately 81% to 95% correspondence with the data obtained from NP and OP swabs. These data suggest that self-collected saliva is an alternative option for the diagnosis of COVID-19.

• International Journal of Internet, Broadcasting and Communication
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• v.13 no.3
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• pp.124-129
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• 2021

As the 4th industrial era is in full swing, the public's interest in related technologies such as artificial intelligence, big data, and block chain is increasing. As artificial intelligence technology is used in various industrial fields, the need for research methods incorporating artificial intelligence technology in related fields is also increasing. Evidence collection among digital forensic investigation techniques is a very important procedure in the investigation process that needs to prove a specific person's suspicions. However, there may be cases in which evidence is damaged due to intentional damage to evidence or other physical reasons, and there is a limit to the collection of evidence in this situation. Therefore, this paper we intends to propose an artificial intelligence-based evidence collection system that analyzes numerous image files reported by citizens in real time to visually check the location, user information, and shooting time of the image files. When this system is applied, it is expected that the evidence expected data collected in real time can be actually used as evidence, and it is also expected that the risk area analysis will be possible through big data analysis.

• Ocean and Polar Research
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• v.45 no.1
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• pp.1-9
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• 2023

Marine microalgae have different collection methods depending on their habitat. In the case of adhesive microalgae, it is difficult to separate organisms from the substrate surface, and contamination is likely to occur during the sampling process. In this study, we analyzed the collection efficiency of adhesive microalgae using three artificial fiber materials (nylon, blend fabric, and viscose rayon). Each fiber showed different fiber diameter and pore characteristics (nylon 26.09 ㎛, blend fabric 56.6 ㎛, viscose rayon 101.3 ㎛). In addition, attached organisms were collected on the surface of artificial substrates at Bukseong Port in Incheon using each tested fiber material. After that, we investigated the population and species composition. The highest number of cells was found in nylon, which was 8 times higher than in the least collected viscose rayon material. In addition, we identified 24 microalgal species from the substrate, demonstrating that the species composition differed from that of surface water. The number of collected microalgae species varied depending on the fiber materials, with nylon containing all the adhesive microalgae. In contrast, only a few microalgae were observed in other fibers. These results suggest that, of the tested fibers, nylon material may be suitable for collecting adhesive microalgae. As a result, this study may be useful for future research on adhesive microalgae.