• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.14-14
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• 2010

Plastid proteomics are essential organelles present in virtually all cells in plants and green algae. Plastids are responsible for the synthesis and storage of key molecules required for the basic architecture and functions of plant cells. The proteome of plastid, and in particular of chloroplast, have received significant amounts of attention in recent years. Various fractionation and mass spectrometry (MS) techniques have been applied to catalogue the chloroplast proteome and its sub-organelles compartments. To better understanding the function of the lumenal sub-organelles within the thylakoid network, we have carried out a systematical analysis and identification of the lumenal proteins in the thylakoid of wheat by using Tricine-SDS-PAGE, and LTQ-ESI-FTICR mass spectrometry followed by SWISS-PROT database searching. We isolation and fractionation these membrane from fully developed wheat leaves using a combination of differential and gradient centrifugation couple to high speed ultra-centrifuge. After collecting all proteins to eliminate possible same proteins, we estimated that there are 407 different proteins including chloroplast, chloroplast stroma, lumenal, and thylakoid membrane proteins excluding 20 proteins, which were identified in nucleus, cytoplasm and mitochondria. A combination of these three programs (PSORT, TargetP, TMHMM, and TOPPRED) was found to provide a useful tool for evaluating chloroplast localization, transit peptide, transmembranes, and also could reveal possible alternative processing sites and dual targeting. Finally, we report also sub-cellular location specific protein interaction network using Cytoscape software, which provides further insight into the biochemical pathways of photosynthesis. The present work helps understanding photosynthesis process in wheat at the molecular level and provides a new overview of the biochemical machinery of the thylakoid in wheat.

• Journal of the Korea Organic Resources Recycling Association
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• v.27 no.4
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• pp.71-81
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• 2019

This research was conducted to improve availability of agricultural by-products, national natural resources that could be used as fertilizer source, by estimating the amount of production and collecting main contents of fertilizer substances from previous studies and data, in order to be used as basic data to investigate national resources that could be used as alternatives for imported oil-cakes in the future. Conversion factor was used to estimate annual production of agricultural by-products for main 34 crop types in 2018. For total agricultural by-products, rice straws accounted at 50.3% for the highest proportion, followed by 8.7% of rice husks, showing that by-products from rice harvest accounted at 59.0% for all by-products. Further, there were listed the contents of fertilizer substances (nitrogen, phosphoric acid, potash) in 40 types of by-products from agricultural areas. The average contents of nitrogen, phosphoric acid, and potash in agricultural by-products were 1.43%, 0.59%, and 1.90%, respectively. As a result of estimating the annual nitrogen supply amount from 26 agricultural by-products in 2018, it was found that it could supply 44,911 tons of nitrogen. The investigated by-products contained fertilizer substances such as nitrogen, phosphoric acid, and potash, but the contents were lower compared to castor oil-cake used as main component of organic fertilizer. Therefore, resource management plan needs to be established for efficient use of by-products because time, location, and nutrient content of agricultural by-product production were extremely different. Research on fertilizer and manure using agricultural by-products need to be conducted to develop and distribute alternatives for imported oil-cakes.

• Cartoon and Animation Studies
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• s.49
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• pp.1-23
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• 2017

3D animation production has had a pivotal position in current animation industry and the necessity of professional management tool for 3D animation production has claimed due to its sophisticated pipeline from advance of technology and global production partnership trend. Shotgun and Ftrack are providing the most appropriate management toolset for 3D animation management among the extant management tools and the efficiency of Shotgun & Ftrack is identified compared with the traditional document oriented management style. The biggest strength of production management using Shotgun is that all of the production staff can directly participate in the communication on the tools therefore they can share the information on Shotgun & Ftrack in real time without constraint of time and location. Moreover, all the process of the production and the history of the discussion on certain production issues are systematically accrue on the tool so that the production history can be easily tracked. Finally, the production management using tools contributes collecting and analysing the production information for the production management team in studios. However, Shotgun & Ftrack has metadata based retrieval method which cost huge amount of effort by human's manual annotation and it also has the limitation of accuracy. In addition, the fact that studios has to have technical professionals first in order to institute the tools into their studios is the actual difficulty of Korean studios when they want to use management tools for their project. Thus, this paper suggests adopting the content-based retrieval system on the tools and tools' expanded technical service for the studios as the solution of the identified issues.

• Journal of Korean Society of Transportation
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• v.27 no.4
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• pp.31-40
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• 2009

Some road charges toll to finance the cost or to manage traffic congestion. With a growth of PPI projects, toll roads would be increase continuously. Tolls have a considerable influence on user's route choice, and sometimes can affect to the departure time and even to mode choice. For modelling toll roads, user's WTP or VOT has an important role and it is general that VOT is equivalent to the wages of workers. The current way of modelling technique yields various toll price elasticity from low to high. When there exist few alternative routes, unrealistic result that all traffic assigned to some shortest path may occur. The toll price elasticity can be influenced by alternative route and congestion level, but some result shows nearly unrealistic patterns. The model to forecast more realistic toll road demand is very essential for estimating toll revenue, choice of optimal toll level & collecting location and establishing toll charge strategy. This paper reviewed some literatures about toll road modelling and tested case study about the assignment technique with different VOT. The case study shows that using different VOT yields more realistic result than the use of single VOT.

• Fire Science and Engineering
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• v.28 no.1
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• pp.44-51
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• 2014

Smoke from fire is a mixture of combustion gases and particles which include micro-droplets formed from condensed organic vapors and carbonaceous agglomerates. The inhalation of smoke particles causes adverse health effects, and it is prerequisite for the hazard and risk analysis of the smoke particles to know how they behaviour in the respiratory tract. The characteristics of the absorption and adsorption of toxic gases and the amount and location of the particle deposition within the respiratory tract that determine the adverse health effects are related to the morphology and the size distribution of smoke particles. In the present work, as a preliminary study for the adverse health effects of smoke particles, the morphologies of the smoke particles from combustible materials were investigated for each fire stage: smouldering, well-ventilated flaming, small under-ventilated flaming, fully-developed under-ventilated fire. The steady-state tube furnace method given in ISO/TS 19700 was used for the generation of smoke particles. The fire stages were controlled by changing furnace temperature and equivalent ratio. The morphologies were analyzed by using Transmission Electron Microscope (Bio-TEM) by collecting the particles on TEM grids put on each stage of a cascade impactor.

• Journal of vocational education research
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• v.33 no.3
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• pp.105-125
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• 2014

The purposes of this study are to develop the standardized tests of career preparation behavior of career preparation behavior for college students. For these, the 'Career Preparation Behavior Scale for College Students' which was developed in 2011 was reviewed and revised. After 609 students were involved and analyzed for the pretest, 1,244 subjects were collected by taking into account gender, grade, major, and location of colleges for developing a standardized test. The Career Preparation Behavior Scale consisted of 3 subareas and 30 items: 11 items for learning area, 12 items for counseling and information collecting area, 7 items for employment action area. The levels of reliabilities, construct validity, discriminant validity, and the concurrent validity were relatively high. Also, the suitability index of the structural model was analyzed to check the structural significance. The degree of career reparation behavior among norm groups was increased in general according to the grades. Scores of students majoring in humanities and social sciences received significantly high scores compared with those of majoring in science and technology or in art and music. But the levels of satisfaction on career preparation behavior were no difference according to gender, grade, and major. 'The Standardized Career Preparation Behavior Scale for College Students' would be used for conducting career education or programs for college students in the future.

• Journal of Auto-vehicle Safety Association
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• v.10 no.4
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• pp.7-12
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• 2018

Injury mechanisms of lower extremity injuries in motor vehicle accidents are focused on fractures, sprains, and contusions. The purpose of this study is to evaluate the analysis of lower extremity injury mechanism in occupant motor vehicle accident by using Hospital Information System (HIS) and reconstruction program, based on the materials related to motor vehicle accidents. Among patients who visited the emergency department of Wonju Severance Christian Hospital due to motor vehicle accidents from August 2012 to February 2014, we collected data on patients with agreement for taking the damaged vehicle's photos. After obtaining the verbal consent from the patient, we asked about the cause of the accident, information on vehicle involved in the accident, and the location of car repair shop. The photos of the damaged vehicle were taken on the basis of front, rear, left side and right side. Damage to the vehicle was presented using the CDC code by analytical study of photo-images of the damaged vehicle, and a trauma score was used for medical examination of the severity of the patient's injury. Among the 1,699 patients due to motor vehicle crashes, 88 (5.2%) received a diagnosis of lower extremity fracture and 141 (8.3%) were the severe who had ISS over 15. Nevertheless during 19 months for research, it was difficult to build up in-depth database about motor vehicle crashes. It has a limitation on collecting data because not only the system for constructing database about motor vehicle crash is not organized but also the process for demanding materials is not available due to prevention of personal information. For accurate analysis of the relationship between occupant injury and vehicle damage in motor vehicle crashes, build-up of an in-depth database through carrying out various policies for motor vehicle crashes is necessary for sure.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.30 no.6
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• pp.235-241
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• 2018

In this study, we collected cases of accidents caused by swell in the east coast of Korea from 2013 to 2017. The location of the accident, the season, the type of coast and the type of damage were classified and the correlation between the accident and the wave data was analyzed by collecting the observation data of the nearby area at the time of the accident. Also, based on the results of the coastal disaster vulnerability assessment of Korea Hydrographic and Oceanographic Agency, the vulnerability grades of swell accidents area were evaluated. In swell accident area, the average grade of the wave exposure index was 4.91, the wave sensitivity index was 3.87, and the wave impact index was 4.90. As a result, most of the swell accidents occurred in the 5 grade (very vulnerable level) of the wave impact index, and the area of the east coast (78.7%) of the same 5 grade was classified into five types according to the wave sensitivity index result. Finally, a countermeasures was taken for characteristics of each type.

• Journal of Cadastre & Land InformatiX
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• v.51 no.1
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• pp.53-65
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• 2021

C-ITS(Cooperative Intelligent Transport System) that pursues traffic safety and convenience uses various sensors to generate traffic information. Therefore, it is necessary to improve the sensor-related technology to increase the efficiency and reliability of the traffic information. Recently, the role of CCTV in collecting video information has become more important due to advances in AI(Artificial Intelligence) technology. In this study, we propose to identify and track dynamic objects(vehicles, people, etc.) in CCTV images, and to analyze and provide information about them in various environments. To this end, we conducted identification and tracking of dynamic objects using the Yolov4 and Deepsort algorithms, establishment of real-time multi-user support servers based on Kafka, defining transformation matrices between images and spatial coordinate systems, and map-based dynamic object visualization. In addition, a positional consistency evaluation was performed to confirm its usefulness. Through the proposed scheme, we confirmed that CCTVs can serve as important sensors to provide relevant information by analyzing road conditions in real time in terms of road infrastructure beyond a simple monitoring role.

• Journal of the Korea Society of Computer and Information
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• v.27 no.8
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• pp.77-84
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• 2022

In this paper, we propose a system for monitoring air pollutants such as fine dust using an unmanned aerial vehicle capable of autonomous navigation. The existing air quality management system used a method of collecting information through a fixed sensor box or through a measurement sensor of a drone using a control device. This has disadvantages in that additional procedures for data collection and transmission must be performed in a limited space and for monitoring. In this paper, to overcome this problem, a GPS module for location information and a PMS7003 module for fine dust measurement are embedded in an unmanned aerial vehicle capable of autonomous navigation through flight information designation, and the collected information is stored in the SD module, and after the flight is completed, press the transmit button. It configures a system of one-stop structure that is stored in a remote database through a smartphone app connected via Bluetooth. In addition, an HTML5-based web monitoring page for real-time monitoring is configured and provided to interested users. The results of this study can be utilized in an environmental monitoring system through an unmanned aerial vehicle, and in the future, various pollutants measuring sensors such as sulfur dioxide and carbon dioxide will be added to develop it into a total environmental control system.