• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1509-1517
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• 2004

A study was conducted to determine the safety of feeding processed broiler litter (BL) to beef cattle. The litter was processed by deepstacking, ensiling and composting. The health issues addressed relevant to the safety of feeding litter included pathogenic bacteria, mycotoxins, heavy metals, medicinal drugs and pesticide residues. Exp. 1 evaluated the feed hygiene of processed rice hulls-bedded BL. The presence of pathogenic bacteria in BL was determined before and after deepstacking. A total of 21 BL samples were collected over a 3-year period of commercial and experimental production of BL for beef cattle. Exp. 2 evaluated the safety of meat of cattle fed deepstacked BL. In Exp. 1, there were no pathogenic bacteria, such as coliform, E. coli, E. coli O157:H7, Salmonella, Listeria and Proteus, in deepstacked BL. Levels of heavy metals (Cu, Fe, Mn and Zn) and toxic heavy metals (As, Pb, Cd and Hg) were lower than the commercial feed tolerances. Aflatoxin, medicinal drug and pesticide residues were detected at extremely low levels. In Exp. 2, the meat of the BL-fed animals exhibited few differences in all analyzed items from that of the control group, showing safety from pathogenic microorganisms and heavy metals. When BL was withdrawn for 14 days prior to slaughtering the BLfed cattle, no medicinal drug residues were detected in the meat. Pesticides in the tissues of either group of animals were much lower than the tolerances. In conclusion, processed rice hulls-bedded BL and the meat of cattle fed BL were safe from the potential hazards of pathogenic bacteria, heavy metals, aflatoxin, medicinal drugs and pesticide residues.

• 한국식품영양학회지
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• 제17권1호
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• pp.60-65
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• 2004

고분자 키토산(분자량 약 800,000)을 농도별(0％, 0.1%, 0.2％, 0.3%)로 김치를 제조하여, pH, 적정산도, 총 균수, 젖산균 수, 대장균군 수 및 관능검사를 조사한 결과는 다음과 같이 나타났다. 키토산 첨가 김치는 대조구에 비하여 pH 저하 및 적정산도의 증가가 늦어지는 것으로 나타나 숙성지연 효과가 있는 것으로 나타났다. 그리고 키토산 첨가(0.3%) 김치는 숙성 6에서 9일까지 대조구에 비하여 총 균수와 젖산균수가 약 1.3 log(cfu/g) 낮은 것으로 나타났다. 발효 15일 후 대장균군 수는 시간과 더불어 그 수가 모든구에서 1.6∼2.3 log(cfu/g)으로 감소하였다. 그리고 관능검사 결과는 키토산 무첨가 김치와 0.1% 첨가 김치는 유사한 선호도를 나타내어 0.1% 첨가 김치가 기능성과 보존성을 높여줄 뿐만 아니라 선호도도 높은 것으로 나타났다.

• 한국축산식품학회지
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• 제18권2호
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• pp.107-114
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• 1998

This study aimed for the examination of quality characteristics and safety of potassium sorbate-free meat products. Therefore, experiments were carried out on the frankfurter sausage and pressed ham, which were stored at 4 and 30$^{\circ}C$ for up to 40 days. The potassium sorbate concentrations of the frankfurter sausage and pressed ham obtained from local market ranged from 1.087 to 1.449g / kg, which were below the permitted value as prescribed in the Korean Hygienic Regulation. At the 0 day the total aerobic bacterial counts of frankfurter sausage and pressed ham were in the level of around 103.0 and 103.4 CFU / g, respectively. However, they were prominently increased after 20 days at 4$^{\circ}C$ and 10 days at 30$^{\circ}C$ to higher than 105 CFU / g. After 30 days the counts were increased to 106.5 and 107.2 CFU / g, respectively. The growth curve of lactic acid bacteria was shown to be similar with that of total bacteria. the counts of lactic acid bacteria of the products stored at 4$^{\circ}C$ were 101∼102 CFU / g lower than those stored at 30$^{\circ}C$. Coliform bacteria was not detected in both of the products stored at 4$^{\circ}C$ even after 40 days storage, but after 10 days at the 30$^{\circ}C$. No significant differences in the microbial counts examined in this study were observed between frankfurter sausage and pressed ham. The biochemical tests on the isolated colonies from Clostridein agar showed no presence of Clostridium botulinum and Clostridium perfringens in the meat products examined. The pH of frankfurter sausage and pressed ham at the beginning was about 6.6, which level was maintained relatively constant during the storage at 4$^{\circ}C$, but it was increased after decrease to about 5.5 during the storage at 30$^{\circ}C$. TBA value was increased slightly till 30 days, but after that time increased sharply. VBN value was increased slowly during the whole storage, but it was more than 30 mg% for the samples stored at 30$^{\circ}C$.

• 한국식품위생안전성학회지
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• 제1권2호
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• pp.133-141
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• 1986

주요 농산 건조가공품에 오염된 미생물의 효과적인 살균법 개발을 목적으로, 현행방법인 ethylene oxide 훈증처리와 감마선 조사에 따른 미생물의 살균효과와 이화학적 품질에 미치는 영향을 검토한 결과는 다음과 같다. 시료의 미생물 오염도는 고추장 분말, 참깨, 느타리버섯 분말, 당근 flake의 순이었으며, 전세균이 $4.6{\times}10^{3}/g~1.5{\times}10^{6}/g$, 내열성 세균이 $1.3{\times}10^{3}/g~1.4{\times}10^{6}/g$, 산저항성 세균이 $8.4{\times}10^{2}.g~8.8{\times}10^{5}/g$, 내삼투압성 곰팡이를 포함한 곰팡이류는 $4.0{\times}10^{2}/g~1.5{\times}104/g$, 대장균군은 당근 flake를 제외한 나머지 시료에서 $4.0{\times}10^{3}/g~2.7{\times}10^{5}/g$ 정도로 오염되어 있어 위생적인 살균처리가 요구된다. 살균효과 실험에서는 대부분의 시료가 7~10 kGy의 감마선 조사로서 모든 미생물의 사멸이 가능하였으나 ethylene oxide 처리구에서는 전반적으로 전세균과 내열성 포자균 및 곰팡이의 살균이 불충분하였다. Ethylene oxide 처리는 시료의 색소, 아미노산, 무기질, 색도 등에 상당한 영향을 미쳤으나 적정선량의 감마선 조사는 이들에 대해 비교적 안전하였으며, 실온에서 3개월 동안 저장된 시료의 전반적인 기호도는 감마선 조사구가 ethylene oxide 처리구나 대조구보다 우수하게 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권2호
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• pp.225-236
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• 2014

Three trials were conducted to evaluate the effect of supplementation of a basal diet with benzoic acid or thymol or a mixture of essential oil blends (MEO) or a combination of benzoic acid with MEO (BMEO) on growth performance of turkey poults. Control groups were fed a basal diet. In trial 1, benzoic acid was supplied at levels of 300 and 1,000 mg/kg. In trial 2, thymol or the MEO were supplied at levels of 30 mg/kg. In trial 3, the combination of benzoic acid with MEO was evaluated. Benzoic acid, MEO and BMEO improved performance, increased lactic acid bacteria populations and decreased coliform bacteria in the caeca. Thymol, MEO and BMEO improved antioxidant status of turkeys. Benzoic acid and BMEO reduced the buffering capacity compared to control feed and the pH values of the caecal content. Benzoic acid and EOs may be suggested as an effective alternative to AGP in turkeys.

• Journal of Animal Science and Technology
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• 제62권5호
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• pp.741-752
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• 2020

Herein, the in vitro protein digestibility of lyophilized Protaetia brevitarsis larvae flour with and without defatting using 70% ethanol was compared with beef loin. Proximate analysis showed that the defatted larvae contained the highest protein content (p < 0.05). The viable counts of total aerobic bacteria, Escherichia coli, and coliform bacteria decreased significantly after defatting the larval samples with 70% ethanol (p < 0.05). Measurement of α-amino group content and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed higher amounts of low molecular weight proteins in the larvae compared to beef loin (p < 0.05). After in vitro digestion, the degree of protein hydrolysis of the digesta was higher for both larvae samples compared to beef loin (p < 0.05). No change was observed in the in vitro larval protein digestibility after defatting. These results highlight the excellent protein digestibility of P. brevitarsis larvae with high protein content. Defatting insect flour with 70% ethanol could enhance microbial safety while maintaining excellent protein digestibility.

• 한국식품영양학회지
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• 제1권1호
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• pp.33-40
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• 1988

A Study was conducted to investigate the effect of feeding Lactobacillus casei YS on the growing performance and gastrointestinal flora of the suckling piglets, which were delivered from 2 heads of three-way crossbred(Landrace$\times$Large White$\times$Duroc) pigs, for 4 weeks. The results from the present study was summarized as follows. Average body weight gains of feeding group was slightly better than that of control group and diarrhea was prevented by successive 7 days feeding. Population levels of lactic acid bacteria were maintained about 107 colony forming unit(cfu) per gram of the contents in both feeding and control group at upper parts of small intestine. In this part, coliform count was greatly reduced in (ceding group but not in control group. pH values of the intestinal contents were gradually decreased especially at the upper part of alimentary track of feeding group. Among lactic acid bacteria, L. salivarius, L. cases and L. fermentum were found most predominant strains in feeding group, Wheareas L. salivarius, L. acidophilus and L. cunts in control group. In the other hand, Escherichia coli recovered from scouring pigs were resistant to the drug such as streptomycin, ampicillin and sensitive to gentamycin and neomycin in vitro test.

• 환경생물
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• 제17권4호
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• pp.521-527
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• 1999

강진만의 생태계의 변화를 조사하기 위해 1998년 2월부터 11월까지 계절별로 주요인 분석을 수행하였다. 이러한 목적을 위해 영양염류, 수온, 염분도, COD, DO, 종속영양세균, 균류 그리고 분변성 대장균 등의 항목들이 6개의 정점에서 조사되었다. 이들 항목을 상관분석과 주요인분석 등의 통계처리를 한 결과 강진만의 생태계는 계절에 따라 2∼4개의 요인에 의하여 영향을 받고 있는 것으로 조사되었다. 그 중에서 특히 담수유입에 따른 영양염류, 부유물질, 염분도 및 수온의 변화 등이 강진만 생태계에 영향을 미치는 요인으로 나타났다. 그리고 다른 만에 비해 강진만에 미치는 환경요인의 영향은 비교적 적었던 것으로 판단되었다.

• Environmental Engineering Research
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• 제19권1호
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• pp.67-73
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• 2014

In India, disposal of vegetable market waste along with municipal solid waste in landfills or dumpsites is creating much nuisance in terms of odor nuisance, leachate production, and greenhouse gas emission into the atmosphere. Therefore, vegetable waste with high biodegradable and nutrient content is composted in a 550-L batch scale rotary drum composter to study the degradation process and its compost properties for its potential reuse as high quality compost. A total 150 kg of working volume was fixed for composting studies with two different ratios, trial A (6:3:1) of C/N 24 and trial B (8:1:1) of C/N 30, respectively. A maximum of $63.5^{\circ}C$ and $61.2^{\circ}C$ was observed in trials A and B; an average of $55^{\circ}C$ for more than 5 days, which helped in the degradation of organic matter and reduction of total and fecal coliform. The temperature dropped suddenly after the thermophilic stage in trial B, and leachate was observed due to insufficient amount of bulking agent. Mesophilic bacteria dominated during the initial stages of composting, and reduced considerably during the thermophilic stage. During the thermophilic stage, the rise in spore-forming organisms, including spore-forming bacteria, fungi, actinomycetes and streptomycetes, increased and these were predominant until the end of the composting process. By examination, it was observed that moisture and leachate production had adverse effects on the compost parameters with higher loss of micronutrients and heavy metals.

• 한국식품조리과학회지
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• 제23권5호
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• pp.749-760
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• 2007

The purpose of this study was to evaluate the microbiological quality of non-heat-processed foods and the effects of HACCP implementation. Here, cabbage salad and cucumber&onion salad were selected and we investigated HA(Hazard Analysis) by checking microbiological quality, time and temperature, pH, and water activity at each processing stage. Thus, the receiving of spices and dressings, washing and sterilizing, cutting, cooking, and serving stages were all considered CCPs. Before implementing HACCP, microbial analysis showed that standard plate counts and coliform counts were higher than standard levels in most of the raw ingredients of each menu, as well as during the production process. The microbiological quality of the utensils and employee's hands used during cooking indicated levels requiring direct management. Evaluations of falling bacteria-in the foodservice establishment work areas ranged from $2{\sim}12CFU/plate$. However, after HACCP implementation, microbiological levels improved to standard levels fly sanitation education. Also, the number of falling bacteria were lower than before implementing HACCP. Therefore, it is essential the foodservice operations make efforts to implement HACCP, so that microbiological hazard levels are lowered and hygienic status improved.