• Journal of Microbiology and Biotechnology
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• v.10 no.4
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• pp.535-538
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• 2000

Streptomyces lavendulae FRI-5 produces the ${\gamma}$-butyrolactone autoregulator IM-2, which is required for nucleoside antibiotic producetion. We have developed a system for introducing DNA into S. lavendule FRI-5 via conjugal transfer from Esherichia cole. Conditions were established for conjugation of the oriT-and attP-containing plasmid pSET152 from E. coli ET12567 (pUZ8002) to FRI-5. Conjugation resulted in integration of the plasmid at the chromosomal C31 attB site. The frequency of intergeneric conjugation varied with the medium used. The highest frequency ($1.6\times10-5$ per recipient) was obtained on ISP medium 2 containing 10mM MgCl2. Southern blot and phenotypic analyses of exconjugants revealed that S. lavendulae FRI-5 contains a unique C31 attB site, and that integration of heterologous DNA into the attB site did not interfere with morphological differentiation or IM-2-dependent signal transduction, including the production of a blue pigment. This system will now enable detailed genetic analysis of the regulation of antibiotic production in S. lavendulae FRI-5.

• Environmental Engineering Research
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• v.14 no.1
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• pp.3-9
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• 2009

Microorganisms play an important role in the geochemical cycles, industry, environmental cleanup, and biotechnology among other fields. Given the high microbial diversity, identification of the microorganism is essential in understanding and managing the processes. One of the most popular and powerful method for microbial identification is comparative 16S rRNA gene analysis. Due to the highly conserved nature of this essential gene, sequencing and later comparison of it against known rRNA databases can provide assignment of the bacteria into the taxonomy, and the identity of its closest relatives. Isolation and sequencing of 16S rRNA genes directly from natural environments (either from DNA or RNA) can also be used to study the structure of the whole microbial community. Nowadays, novel sequencing technologies with massive outputs are giving researchers worldwide the chance to study the microbial world with a depth that was previously too expensive to achieve. In this article we describe commonly used research approaches for the study of individual microorganisms and microbial communities using the tools provided by Ribosomal Database Project website.

• Journal of Korean Elementary Science Education
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• v.32 no.4
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• pp.379-392
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• 2013

The purpose of this study was to examine the effects of animation-based instruction on elementary students' level of understanding and interest on a plant's structure and function, using "Magic School Bus(Joanna Cole, Bruce Degen, 1986)". The understanding and interest measurements were administered to 99 fifth grade students at a elementary school located in Seoul. The study examined the changes in understanding and interest through pre-test and post-test of the two groups. Intensive interviews were conducted to find factors that enhance understanding and interest. Three key findings were revealed from the results. First, the animation-based instruction enhanced the level of understanding in the experimental group compared to the control group. Second, animation-based instruction made high-achieving students enhance their interest in topic component and attitude component. And, animation-based instruction did not led low-achieving students to increase their interest in the topic component and attitude component. We suggest that animation-based instruction positively influences students' understanding, but its effects on students' interest are dependent on their conceptual understanding of the topic.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.2
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• pp.30.1-30.1
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• 2016

We have estimated a metallicity map of the Large Magellanic Cloud (LMC) using the Magellanic Cloud Photometric Survey (MCPS) and Optical Gravitational Lensing Experiment (OGLE III) photometric data. This is a first of its kind, high-spatial resolution map of metallicity up to a radius of $4^{\circ}-5^{\circ}$, derived using large area photometric data and calibrated using spectroscopic data of Red Giant Branch (RGB) stars. The RGB is identified in the V, (V - I) colour- magnitude diagrams of small subregions of varying sizes in both data sets. The slope of the RGB is used as an indicator of the mean metallicity of a subregion, and it is calibrated to metallicity using spectroscopic data for field and cluster red giants in selected subregions. The mean metallicity of the LMC is found to be [Fe/H] = -0.37 dex (${\sigma}[Fe/H]=0.12$) from MCPS data, and [Fe/H] = -0.39 dex (${\sigma}[Fe/H]=0.10$) from OGLE III data. The bar is found to have an uniform and higher metallicity compared to the disk, and is indicative of an active bar in the past. Both the data sets suggest a shallow radial metallicity gradient up to a radius of 4 kpc ($-0.049{\pm}0.002$ dex kpc-1 to $-0.066{\pm}0.006$ dex kpc-1). This metallicity gradient of the LMC disk, though shallow, resembles the gradient seen in spiral galaxies, and similar to that found in our Galaxy.

• Phonetics and Speech Sciences
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• v.2 no.1
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• pp.41-50
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• 2010

The current study exploresacoustic variation induced by prosodic contexts in different speech styles,with a focus on motherese or child-directed speech (CDS). The patterns of variation in the acoustic expression of voicing contrast in English stops, and the role of prosodic factors in governing such variation are investigated in CDS. Prosody-induced acoustic strengthening reported from adult-directed speech (ADS)is examined in the speech data directed to infants at the one-word stage. The target consonants are collected from Utterance-initial and -medial positions, with or without focal accent. Overall, CDS shows that the prosodic prominence of constituents under focal accent conditions variesin the acoustic correlates of the stop laryngeal contrasts. The initial position is not found with enhanced acoustic values in the current study, which is similar to the finding from ADS (Choi, 2006 Cole et al, 2007). Individualized statistical results, however, indicate that the effect of accent on acoustic measures is not very robust, compared to the effect of accent in ADS. Enhanced distinctiveness under focal accent is observed from the limited subjects' acoustic measures in CDS. The results indicate dissimilar strategies to mark prosodic structures in different speech styles as well as the consistent prosodic effect across speech styles. The stylistic variation is discussed in relation to the listener under linguistic development in CDS.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.11 no.2
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• pp.49-54
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• 1978

The cold water mass appeared in offshore of the east coast of Korea in summer season was studied in aspect of chemical oceanography. Such a typical relationship between phosphate and dissolved oxygen as shown in the upwelling regions could not be found in the east coast except around the Kampo coast, southern part of the east coast. It is possible to isolate the North Korean Cold Water from tile proper water of tile Japan Sea by using $\sigma_t-O_2$ diagram. The origin of the cold water mass in offshore of the east coast of Korea in summer is not mainly due to the development of upwelling of the proper water of the Japan Sea but thesouthwardflolvingoftheNorthKoreanCold Water.

• Proceedings of the Korean Nuclear Society Conference
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• 1997.10a
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• pp.772-777
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• 1997

Previous MELCOR and SCDAP/RELAP5 nodalizations for simulating the counter-current, natural circulation behavior of vapor flow within the RCS hot legs and SG U-tubes when core damage progress can not be applied to the steady state and water-filled conditions during the initial period of accident progression because of the artificially high loss coefficients in the hot legs and SG U-tubes which were chosen from results of COMMIX calculation and the Westinghouse natural circulation experiments in a 1/7-scale facility for simulating steam natural circulation behavior in the vessel and in the hot leg and SG during the TMLB' scenrio. The objective of this study is to develop a natural circulation modeling which can be used both for the liquid flow condition at steady state and for the vapor flow condition at the later period of in-vessel core damage. For this, the drag forces resulting from the momentum exchange effects between the two vapor streams in the hot leg was modeled as a pressure drop by pump model. This hot leg natural circulation modeling of MELCOR was able to reproduce similar mass flow rates with those predicted by previous models.

• Archives of Plastic Surgery
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• v.49 no.3
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• pp.440-443
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• 2022

Spinal accessory nerve (SAN) palsy is typically a result of posterior triangle surgery and can present with partial or complete paralysis of the trapezius muscle and severe shoulder dysfunction. We share an atypical case of a patient who presented with SAN palsy following an injury sustained playing competitive volleyball. A 19-year-old right hand dominant competitive volleyball player presented with right shoulder weakness, dyskinesia, and pain. She injured the right shoulder during a volleyball game 2 years prior when diving routinely for a ball. On physical examination she had weakness of shoulder shrug and a pronounced shift of the scapula when abducting or forward flexing her shoulder greater than 90 degrees. Manual stabilization of the scapula eliminated this shift, so we performed scapulopexy to stabilize the inferior angle of the scapula. At 6 months postoperative, she had full active range of motion of the shoulder. SAN palsy can occur following what would seem to be a routine volleyball maneuver. This could be due to a combination of muscle hypertrophy from intensive volleyball training and stretch sustained while diving for a ball. Despite delayed presentation and complete atrophy of the trapezius, a satisfactory outcome was achieved with scapulopexy.

• Journal of the East Asian Society of Dietary Life
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• v.13 no.3
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• pp.197-215
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• 2003

This study was carried out to investigate the effects of added emulsifier and enzyme on the quality characteristics of Seolgiddeok with black rice powder, green tea powder, Lentines edodes powder, pumpkin powder during storage. Samlpes were divided into two groups, the emulsifier-enzyme added group and the control, and stored fur 4 days at 2$0^{\circ}C$. Moisture content was measured by the air oven method; the Hunter's cole. values by Lightness (L), redness (a), yellowness (b) value; the degree of gelatinization by the enzyme digestion method, and expressed as glucose content by Somogi-Nelson method. The degree of retrogradation was calculated as the retrogradation rate using the degree of gelatinization value, and total cell and mold counts were also measured. Texture and sensory characteristics were also investigated. The results are as follows: The emulsifier-enzyme group was significantly different from the control from each examination and the retrogradation of pumpkin Seolgiddeok with emulsifier-enzyme was delayed the most among samples tested.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.2
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• pp.66-81
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• 1989

The characteristics of resilient modulus(Mr) which dominates the life of pavement and the design of pavement were investigated on the test specimens which were cement treated and non-treated of the three different soil types. The results are summarized as follows : 1. The resilient modulus was decreased by increasing the cyclic deviator stress ($\sigma$d) , especially the resilient modulus was gradually decreased or sometimes increased when the value of ad was greater than 0.75- 1. 0kg/cm$^2$. 2. The resilient modulus was increased by increasing the homogeneous confined stress ($\sigma$do) and such phenomena were distinct on the coarse soils. 3. The resilient modulus was increased by increasing the ratio of confined stress(Kc), and this phenomena were eminent on the coarse soils too, and the higher permanent strain was showed by increasing the value of Kc. 4. In the drained cyclic triaxial compression test, the value of ad, Kc, and (Oho) was introduced by the following interrelated equations which were similar to the Mr model of Cole. Kcn/Mr=K1(J$_2$/ $\tau$oct)K2 ............. (coarse soli) Mcn/Mr=K3($\sigma$dp/ $\tau$f)k4 ...............(fine soils) 5. The stress path was not much affected by the value of Mr, however, moisture content, dry desity, and contant of fines affected the value of Mr. 6. In the soil-cement specimens, the resilient compression strain($\varepsilon$d) was decreased by the increment of the $\sigma$ho, and Mr was decreased by increasing the $\sigma$d 7. In the flexible pavement. the cement treated layer should be designed not to fail by the fatigue before the designed traffic load, and actually the pavement could cover the traffic load to a certain extent under the post-crack phase, therefore farther studies on this phenomena' are required in the design analysis. 8. The finite element computer program (ANALYS) was used for displacement analysis of pavement containing the cement-treated layer, The result showed that the program used for this analysis was proved to be usable.