• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.1
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• pp.13-16
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• 2003

Seeds of burcucumber were treated with accelerated aging, cold-stratification, and light quality illuminated during desiccation to enhance their germination and seedling emergence. The germination was increased by aging and cold-stratification although the latter treatment showed greater effectiveness than the former one. In the combined treatment of aging 6 days at $45^{\circ}C$ and cold-stratification, the germination was promoted under longer period of cold-stratification to reach nearly 100% in 3 week cold-stratification on the ninth day from sowing. In the sequentially combined treatment of aging, cold-stratification, and light quality during 24 hour desiccation at $35^{\circ}C$, no-stratified seeds showed the highest rate in red light treatment but the lowest in far-red light. This implies that the phytochrome action run during the desiccation of imbibed seeds. The red light exposure during drying for the cold-stratified seeds after aging accelerated the germination even more than the dark treatment and germinated 100% on the next day of sowing. It is concluded that the sequential treatment of aging, cold-stratification, and red light illumination during desiccation can highly promote percentage and speed of burcucumber seed germination.

• Horticultural Science & Technology
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• v.34 no.4
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• pp.557-563
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• 2016

To improve the germination of Spiraea fritschiana seeds for mass propagation, we evaluated the effect of a range of temperatures, cold stratification periods, and gibberellic acid ($GA_3$) treatments on three germination characteristics. Final germination percentage (FGP) increased as the temperature for seed germination increased, up to $30^{\circ}C$, while the mean germination time (MGT) and the mean number of days to 30% germination ($T_{30}$) decreased when seeds were germinated at $25-30^{\circ}C$. The optimum germination temperature of S. fritschiana seeds is approximately $30^{\circ}C$ considering FGP, MGT, and $T_{30}$ together. FGP increased with the duration of cold stratification up to a period of 6 weeks, but declined after 8 weeks of cold stratification, as prolonged cold stratification can induce dormancy with a resultant decline in germination. Pretreatment with 6-8 weeks of cold stratification or soaking seeds in distilled water or $500mg{\cdot}L^{-1}$ $GA_3$ for 24 h accelerated and increased the germination of S. fritschiana seeds, regardless of temperature. However, further study might be required to evaluate the effect of $GA_3$ concentrations lower than $500mg{\cdot}L^{-1}$ on the promotion of germination in S. fritschiana seeds.

• Proceedings of the KSME Conference
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• 2002.08a
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• pp.51-54
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• 2002

When a cold HPSI (High Pressure Safety Injection) fluid associated with an design basis accident, such as LOCA (Loss of Coolant Accident), enters the cold legs of a stagnated primary coolant loop, thermal stratification phenomena will arise due to incomplete mixing. If the stratified flow enters a reactor pressure vessel downcomer, severe thermal stresses are created in a radiation embrittled vessel wall by local overcooling. Previous thermal-mixing analyses have assumed that the thermal stratification phenomena generated in stagnated loop of a partially stagnated coolant loop are neutralized in the vessel downcomer by strong flow from unstagnated loop. On the basis of these reasons, this paper presents the thermal-mixing analysis results in order to identify the fact that the cold plume generated in the vessel downcomer due to the thermal stratification phenomena of the stagnated loop is affected by the strong flow of the unstagnated loop.

• Journal of Mechanical Science and Technology
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• v.17 no.9
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• pp.1380-1387
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• 2003

When a cold HPSI (High pressure Safety Injection) fluid associated with an overcooling transient, such as SGTR (Steam Generator Tube Rupture), MSLB (Main Steam Line Break) etc., enters the cold legs of a stagnated primary coolant loop, thermal stratification phenomena will arise due to incomplete mixing. If the stratified flow enters the downcomer of the reactor pressure vessel, severe thermal stresses are created in a radiation embrittled vessel wall by local overcooling. As general thermal-hydraulic system analysis codes cannot properly predict the thermal stratification phenomena, RG 1.154 requires that a detailed thermal-mixing analysis of PTS (pressurized Thermal Shock) evaluation be performed. Also. previous PTS studies have assumed that the thermal stratification phenomena generated in the stagnated loop side of a partially stagnated primary coolant loop are neutralized in the vessel downcomer by the strong flow from the unstagnated loop. On the basis of these reasons, this paper focuses on the development of a 3-dimensional thermal-mixing analysis model using PHOENICS code which can be applied to both partial and total loop stagnated cases. In addition, this paper verifies the fact that, for partial loop stagnated cases, the cold plume generated in the vessel downcomer due to the thermal stratification phenomena of the stagnated loop is almost neutralized by the strong flow of the unstagnated loop but is not fully eliminated.

• Journal of Forest and Environmental Science
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• v.38 no.4
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• pp.284-289
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• 2022

This study was conducted to determine the seed dormancy types of Sambucus racemosa subsp. pendula Nakai and S. williamsii Hance. Low temperature stratification (1, 2, 3, 4 months) and GA3 treatment (1,000 mg/L) were performed on seeds to determine the type of seed dormancy. After the treatment, seeds were placed on a petri dish at 25℃ under light conditions. The germination rate and mean germination time were investigated. Results showed that cold stratification was effective in breaking the dormant state of the seed in both species. In the low temperature stratification treatment, the seed germination performance was improved as the treatment period was prolonged. Gibberellin treatment was effective in breaking the dormant state of S. racemosa subsp. pendula without low temperature stratification. However, S. williamsii did not break the dormant state of the seed by gibberellin treatment without low temperature stratification treatment. In the gibberellin treatment, germination performance was improved according to the low temperature stratification treatment period. As a result of this study, the seeds of S. williamsii have both an intermediate complex and a deep complex morphophysiological dormancy (MPD). In comparison, it was found that the S. racemosa subsp. pendula had intermediate composite MPD.

• Journal of ILASS-Korea
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• v.8 no.1
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• pp.23-28
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• 2003

When a cold HPSI (High Pressure Safety Injection) fluid associated with a design basis accident, such as LOCA (Loss of Coolant Accident), enters the cold legs of a stagnated primary coolant loop, thermal stratification phenomena may arise due to incomplete mixing. If the stratified flow enters a reactor pressure vessel downcomer, severe thermal stresses are created in a radiation embrittled vessel wall by local overcooling. Previous thermal-mixing analyses have assumed that the thermal stratification phenomena generated in stagnated loop of a partially stagnated collant loop are neutralized in the vessel downcomer by strong flow from unstagnated loop. On the basis of these reasons, this paper presents the thermal-mixing analysis results in order to identify the fact that the cold plume generated in the vessel downcomer due to the thermal stratification phenomena of the stagnated loop is affected by the strong flow of the unstagnated loop.

• Journal of Environmental Science International
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• v.32 no.1
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• pp.77-88
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• 2023

Kadsura coccinea (Lem.) A.C. Smith is used as a medicinal plant and cosmetic material in China and Southeast Asia. To mass-produce Kadsura coccinea seedlings, the effects of gibberellic acid (GA₃) and cold stratification treatments on seed germination were investigated. Seed germination rate with GA₃ treatment was most effective at concentrations of 250 or 500 mg/L. With respect to mean germination time (MGT), mean daily germination, and T₅₀ (days to reach 50% seed germination), the germination-promoting effect was improved as the concentration of GA₃ increased. Stem growth of seedlings was the highest following GA₃ treatments of 250 and 500 mg/L, and the growth promoting effect gradually decreased as the concentration of GA₃ decreased. Root growth was stimulated at GA₃ concentrations of 250-1,000 mg/L. Examination of the effect of stratification treatment for 15, 30 and 60 days at temperatures of 0, 5 and 10℃ on the germination rate revealed that the most stratification treatment temperature was 10℃, and the results improved with longer treatment periods. Altogether, GA₃ and stratification treatments improved the seed germination rate, shortened the MGT, improved germination uniformity, and produced healthy seedlings.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.253-258
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• 2015

In South Korea, Pinus pumila (Pall.) Regel (dwarf stone pine) has been designated as a critically endangered species by the Korea Forest Service. We have difficulties in obtaining the seeds of P. pumila because P. pumila grows only in the Daecheongbong area (1550–1700 m above sea level) of Mt. Seorak and almost all of its cones are damaged by birds and rodents. For establishing an ex situ conservation stand of P. pumila, this study was conducted to figure out the effects of wet cold (cold stratification, prechilling) and GA₃ treatment on the germination of P. pumila seeds. After cold stratification (1, 2, 3, 4, 5 months), prechilling (1, 2, 3, 4, 5 months) and GA₃ treatment (0, 100, 500, 1,000, 2,000, 3,000 ㎎/L), seeds were placed on petri-dishes at 25℃ under light condition. The percentage of germination, mean germination time and the germination rate were investigated. The results showed that both of the cold stratification and prechilling were effective in improving germination performances. However, there were no significant differences in performances between the two cold treatments. Within each treatment, the germination performances improved with the period of treatment. However, after three months of treatment, the performances showed no significant improvement. The gibberellin treatment was also effective in improving seed germination of P. pumila. The percentage of germination reached 79.0% in the seeds treated with 100 ㎎/L of GA₃. However, the germination performances decreased at high concentration of GA₃ treatments (over 2000 ㎎/L). In conclusion, cold stratification (over 3 months) or 100 ㎎/L of GA₃ treatment was considered to be the appropriate method for seedling production of P. pumila.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.29 no.12
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• pp.654-662
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• 2017

When emergency core cooling system (ECCS) is operated during loss of coolant accident (LOCA) in a pressurized water reactor (PWR), pressurized thermal shock (PTS) phenomenon can occur as cooling water is injected into a cold leg, mixed with hot primary coolant, and then entrained into a reactor vessel. Insufficient flow mixing may cause temperature stratification and steam condensation. In addition, flow vibration may cause thermal stresses in surrounding structures. This will reduce the life of the reactor vessel. Due to the importance of PTS phenomenon, in this study, calculation was performed for Test 1 among six types of OECD/NEA ROSA tests with ANSYS CFX R.17. Predicted results were then compared to measured data. Additionally, because temperature difference between the hot coolant at the inlet of the cold leg and the cold cooling water at the inlet of the ECCS injection line is 200 K or more, buoyancy force due to density difference might have significant effect on thermal-hydraulic characteristics of flow. Therefore, in this study, the necessity to include buoyancy force term in governing equations for accurate prediction of single phase thermal stratification in both cold legs and downcomer by ECCS injection was numerically studied.

• Horticultural Science & Technology
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• v.34 no.6
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• pp.830-839
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• 2016

Germination of Hemerocallis seeds as influenced by cold stratification at $5^{\circ}C$ (CS) and maturity of seeds evaluated using X-ray imaging has not been well investigated. Seeds of H. lilioasphodelus, H. citrina, H. citrina 'April Flower', and H. minor collected from China and H. thunbergii collected from Korea were germinated at $20^{\circ}C$ without pre-temperature treatment, while H. hongdoensis, H. dumortieri, H. minor, and H. vespertina seeds were treated with CS. Harvesting 'Stella de Oro' capsules at 35-40 days after anthesis yielded mature seeds with well-developed embryo and cotyledons analyzed by X-ray images with a 92% germination in 17 days after sowing. Seeds of H. thunbergii and H. citrina germinated in less than 13 days without CS; two weeks of CS did not accelerate seed germination. Seeds of H. hongdoensis germinated in 24 days when seeds were stored at $25^{\circ}C$ without CS and in less than 27 days when cold stratified. Therefore, 'Stella de Oro' capsules should be harvested at 35-40 days after anthesis to harvest mature seeds. Cold stratification is not required to accelerate seed germination in the Hemerocallis taxa evaluated in this study.