• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.188-199
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• 1996

The native ecological environment and aromatic constituents of Korean wild Codonopsis lanceolata and one Japanese strain were investigated to find Codonopsis lanceolata strains showing high aromatics, and to know regional differences among these strains. The results were as follows : There were no remarkable differences among the Korean wild C. lanceolata strains in ecological environments. Recovery yield of essential oils was highest in Togyusan strain with 0.009%. Difference in protein band patterns among these strains was not recognized, and peroxidase and esterase pattern changes were appeared in different collected regions at the leaf and root tissues. Major aromatic constituents were 11 kinds of aliphatic alcohols such as trans-2-hexenal, 1-hexanol, cis-3-hexanol, and trans-2-hexanol. And Togyusan strain, Sobaeksan strain, and Kayasan strain have the highest aliphatic alcohols of plant essential oils. In particular, BHT(butylated hydroxytoluene), one of the antioxidants, was detected in Chirisan strains.

• Korean journal of applied entomology
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• v.45 no.3 s.144
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• pp.339-346
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• 2006

Total 90 species of medicinal plants were surveyed to see if they have any suppressive effects on the dinsity of M hapla at the exhibition field in the Chinan medicinal herbs experiment station. In 70 species including Achyranthes japonica, root-knot and/or egg sac of M. hapla was not found and these plants were planted in C. lanceolata field to check the degree of M. hapla infection. In 26 species including A. japonica, M. hapla infection was not observed. Simultaneously, 30 species were planted in pots to find out degree of infection by M. hapla. Dianthus chinensis, Rudbeckia bicolor, Sedum kantschaticum, Ricinus communis, Anemarrhena asphodeloides, Malva verticillate, Chelidonium majus, Sesamum indicum, Agrimonia pilosa, Geum aleppicum, Sanguisorba officinalis and Scrophularia buergeriana were free from infection. While the number of galls and density of M. hapla in soil were higher to high innoculation density, and the growth of C. lanceolata was rower.

• Journal of Bio-Environment Control
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• v.27 no.1
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• pp.13-19
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• 2018

This study was conducted to examine the germination and growth during seedling period of Codonopsis lanceolata and Astragalus membranaceus as affected by different media. The seeds were sown in commercial medium (Tosilee), coir, rockwool, and urethane sponge. The seeds were germinated for 22 days in a closed-type plant production system, and seedlings were grown for 35 days after sowing in the venlo-type glasshouse located on Gyeongsang National University. Nutrient solution was supplied by the sub-irrigation as EC $1.0dS{\cdot}m^{-1}$ and pH 6.5 every second day. Final germination of Codonopsis lanceolata was the significantly highest in the coir and rockwool media as 68.5% and 67.9%, respectively. Mean daily germination was also the highest in the coir and rockwool media as 4.2 and 4.1, respectively. The germination rate of Astragalus membranaceus was not significantly difference in the media types. Plant height and leaf area of Codonopsis lanceolata were the significantly highest in the rockwool medium as 11.5 cm and $11.3cm^2$, respectively. Longest root length and fresh weight of root were the greatest in the coir medium as 5.8 cm and 0.07 g, respectively. Plant height and leaf area of Astragalus membranaceus were the significantly highest in the coir medium as 14.0 cm and $16.9cm^2$, respectively, and fresh and dry weights of root were the highest in the urethane sponge medium as 0.34 g and 0.03 g, respectively. Therefore, these result suggest that the rockwool and coir media were suitable for the germination and growth during seedling period of Codonopsis lanceolata and Astraglus membranaceus.

• The Korean Journal of Food And Nutrition
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• v.32 no.6
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• pp.630-635
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• 2019

The purpose of this study was to determine the content of polyphenols and flavonoids, vitamin C, and antioxidant activity for the extract from the Deodeok sprout. To accomplish this, the Deodeok sprout whole (CLS-W), above ground part (leaf, stem, CLS-L), and root (CLS-R) were individually extracted using 70% ethanol. The highest levels of total polyphenols and total flavonoids were observed in the Deodeok sprout extract CLS-L2. Similarly, antioxidant activities resulting in radical scavenging activities increased significantly in the extract of CLS-L2. In conclusion, these results indicate that Deodeok sprouts can be used as a viable, new natural antioxidant source.

• Korean Journal of Pharmacognosy
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• v.6 no.1
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• pp.43-47
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• 1975

Attempts were made to determine the characteristics of the morphology of Codonopsis lanceolata $T_{RAUT's}$ root. Its saponin and crude powder were examined by foaming and hemolytic tests and by observing transverse sections: 1) The cork-layer was consisted of multiple cells, and some of the cork cells contained monoclinic prism crystals of calcium oxalates. 2) The vascular bundle was formed of radial arrangement by the preminentic bursting pith and the irregular cambium ring with the abnormal growth. 3) The latex vessel and latex of the cortex were brown-gray or yellowish-brown. The latex vessel and sieve tube were consisted of the independent aggregates which had a number of layers. It had much more independent aggregates than Platycodi Radix. 4) The xylem was less than the phloem portion. The thick-walled vessels which had one to five lines ware more distributed than in Platycodi Radix. 5) The inulin distribution on a piece of section which is dipped in alcohol was observed through out the tissues, but starch grain was not found in it. 6) The duration time of foaming reaction of the crude powder in test tubes was shorter than that of Platycodi Radix. Foaming index of the foaming test of five percent decoctions was 3.33, 7) In the hemolytic test which used 10 percent decoction and one percent saponin solution, hemolytic action was very weak.

• Korean journal of food and cookery science
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• v.15 no.6
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• pp.555-564
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• 1999

The objective of this study was to investigate the preference of root vegetables and the inhibitory effect of the vegetables on harmful enzymes of intestinal bacteria. Two hundred fifty respondents in Seoul area surveyed to obtain information from Sep. 30 to Oct. 30, 1998. Respondents preferred Inpuomoea batatas (sweet potato, 4.05), Solanum tuberosum(potato, 3.97), Allium cepa(onion, 3.68), Codonopsis lanceolata(3.64) and Raponus sativus(redish, 3.60). The growth of B. breve K-110 was effectively increased by adding 0.5% extract of Solanum tuberosum(139%), Codonopsis lamceolate(145%), Dioscorea japonica(164%), Colocisia antiquorum(144%) extract to the medium. B. breve K-100 for beneficial bacteria, and E. coli HGU-3 or Bacteroides JY-6 for harmful bacteria were used to determine the inhibitory effect of root vegetables on harmful intestinal enzymes after co-culturing harmful and beneficial bacteria. The extract of Solanum tuberosum, Codonopsis lanceolata, Dioscorea japonica (yam) and Colocisia antiquorum (taroes) showed inhibitory effect on ${\beta}$-glucuronidase and tryptophanase of intestinal bacteria. The macromolecules were isolated from Solanum tuberosum, Codonopsis lanceolata, Dioscorea japonica and Colocisia antiquorum by Sephadex G-100 column chromatography. By adding these isolated marcromolecules to the medium, the growth of B. breve K-100 were also increased and high inhibitory effects on the ${\beta}$-glucuronidase and tryptophanase were measured. These results suggested that the harmful enzymes of intestinal bacteria were inhibited by consuming Solanum tuberosum, Codonopsis lanceolata, Dioscorea japonica and Colocisia antiquorum. Therefore, they could prevent gastrointestinal diseases.

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.04a
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• pp.197-197
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• 2018

• Korean journal of applied entomology
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• v.44 no.4 s.141
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• pp.317-323
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• 2005

This study was conducted to survey plant-parasitic nematodes and their damages on Codonopsis lanceolata Trautv at 116 fields of 15 major cultivation areas in Korea. Among the 9 plant-parasitic nematode genera detected in the fields, Ditylenchus citri, Meloidogyne hapla, M. incognita, Pratylenchus neglectus, Tylenchorynchus clayton were identified. M. hapla Chitwood showing the highest field infection rate of 61.9% followed by Tylenchus spp. 16.1%, Pratylenchus neglectus 7.8%, Ditylenchus citri 5.1%, and Helicotylenchus sp. 5.1%. Average field infection rate of root-knot nematodes in 2-year-old C. lanceolata was 67.0%, and the average plant infection rate was 60.2%. The average yield decrease rate was 10%. The damage rate by the root-hot nematode increased as the cultivation year extended in Jeonbuk province, and the damage was also higher in flat land than in mountainous areas.

• Korean Journal of Plant Resources
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• v.29 no.3
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• pp.347-354
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• 2016

The present study was performed to investigate the effects of the colchicine concentrations on chromosome doubling for producing of tetraploid plants of Codonopsis lanceolata, and its effect on plant morphology. A total of 180 individuals germinated from 16 treatment groups, were exposed to various concentrations (0.05-1.0% w/v) of colchicine for different soaking duration (3-24 hour). The highest numbers of tetraploid plants (3) were observed from the lowest concentration of colchicine (0.05%), and one (1) tetraploid plant was obtained from the 0.5% concentration group with a 6 hour treatment. However, no tetraploid individual was observed in any other treatment groups. The plant height of the diploid (18.1 ㎝) was slightly shorter than that of the tetraploid (13.4 ㎝). The fresh weight of the main root in the diploid (0.5 g) was four-fold higher than the tetraploid (2.2 g). The colchicine-treated plant regeneration rate in C. lanceolata was decreased when the plants were subjected to high concentration of colchicine. In particular, the highest number of tetraploid plants (5 and 3) was obtained from the lower concentration (0.05% and 0.1%) of colchicine for 6-hour treatment, which were a higher rate (29.4% and 30%) of regenerated tetraploid plants than other regenerated plants. As in the seed treatment result, the plant height of the diploid was significantly higher (10.4 ㎝) than tetraploid. The higher morphological changes were observed comparatively from tetraploid plants than the diploid.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.239-247
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• 2004

A cyclophilin 1 cDNA clone(GenBank accession no.CF924191) was isolated from the taproot of C. lanceolata and designed as C1CyP1. Determination of the nucleotide sequence of C1CyPl identified an open reading frame of 525bp, which shared high homologies with cyclophilins that were previously reported in other organisms. The C1CyP1 amino acid sequence possesses 7 amino acid residue stretch(KSGKPLH) that is characteristic of plant cytosolic dehydrins. Currently available amino acid residues of plant cyclophilins were compared to examine their phylogenetic relationship to C1CyP1. In the phylogenetic analysis, based on the aligned sequences, C1CyP1 showed high homology with arabidopsis ROC2 and rice CyP1. The transcript that corresponded to C1CyP1 was abundant in callus, but only basal level of transcript was detected in stem, leaf and root. For the study in the defense mechanism against various stresses, we report expression patterns of this gene by quantative RT-PCR.