• Korean Journal of Medicinal Crop Science
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• v.6 no.4
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• pp.239-244
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• 1998

This experiment was to determine the effects of soil pH on crude components and aromatic constituents of Codonopsis lanceolata. Vine length of Codonopsis lanceolata grown at soil pH of 6.5 was longer(299cm) compared to that grown under other soil pHs. Fresh root weight increased at soil pH of 5.5 and 6.5, showing 17.9 and 15.6g per plant, respectively. Contents of crude components such as protein, fat, fiber, and ash decreased as soil became alkalized and crude ash content ranged from 2.99% to 3.85 %, showing a similar response to soil pH. Forty-eight volatile aromatic compounds in the root of Codonopsis lanceolata were identified by GC/MS. Major aromatic compounds were 1-hexanol, cis-3-hexanol, and trans-2-hexanol. In particular, trans-2-hexanol was highest in soil pH of 6.5, attaining the 156.87% area. Essential oil content was also highest at soil pH of 6.5 with 0.007%. As a result, it was considered that the soil pH of 6.5 was most effective for the improvement of essential oil and aromatic constituents in the roots of Condonopsis lanceolata Trautv.

• Journal of Plant Biotechnology
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• v.36 no.2
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• pp.124-129
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• 2009

In general, the root color of Codonopsis lanceolata is white, but red or blue-colored root is found at a low frequency in nature. Red or blue-colored roots have scarcity value, thus farmers wish to produce colored roots. The factors for determining the color of roots are unclear whether the color is controlled by genetically or simply by environmentally such as soil environment. Using in vitro culture system which is advantageous for setting of the same culture condition, we analyzed the physiological and morphological characteristics and genetic differences among red-, blue- and white lines of C. lanceolata. In the red colored roots, stems of in vitro cultured plantlet were colored in dark red pigment. Histological analysis revealed that the red pigment was accumulated in the outer cortex layer of the stem and determined as anthocyanin. Chlorophyll contents in red root lines were higher than those in white- and blue root lines. Plantlets from red roots were smaller in both shoot length and total leaf area than those from white- and blue roots. Genetic differences among the three different colored C. lanceolata were determined by RAPD (Randomly Amplified Polymorphic DNA) analysis. Each line of colored roots had clear DNA polymorphism. These results indicate that the occurrence of red- and blue colored roots in nature was determined by genetic factors rather than soil enviromental conditions.

• Plant Resources
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• v.6 no.3
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• pp.227-232
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• 2003

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Codonopsis lanceolata. Three tetraploid plants of C. lanceolata were produced from seeds which absorbed 0.1 % colchicine solution for 12 hours, and 0.5% colchicine solution for 1 and 6 hours from seedlings, respectively. But tetraploid was not produced from shoot tips treated by colchicine solution. Compared to diploid, tetraploid plants had larger stomata, but less number of stomata. Fresh weight of tetraploid plants was 1.4∼3.6 times heavier than diploid plants.

• The Korean Journal of Pesticide Science
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• v.20 no.2
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• pp.165-171
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• 2016

Platycodon grandiflorum and Codonopsis lanceolata have been considered as cash vegetables and alternative medicine plants in Korea. In a previous survey from 1998 to 2010, repeated cultivation of the plant increased root rot disease incidence and severity. The disease has been recognized as critically limiting factor for crop production. However, control method has not been established for the disease of P. grandiflorum and C. lanceolata. In this study, control efficacy of Tebuconazole EC, Trifloxystroim SC and grapefriut extract was evaluated on the root rot disease of these plants in two different field conditions. Three of different fungicide were non critical effect to the disease severity and the control value, but grapefriut seed extract (GSE) was more ability to control the disease in C. lanceolata. In most things, soil drainage was the most important to decrease the disease severity and to improve the control value.

• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.265-270
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• 2015

The roots of Codonopsis lanceolata (Campanulaceae) contain several kinds of triterpenoid saponin with high medicinal values, which have been used in traditional medicines. This study investigates the impacts of methyl jasmonate (MeJA) - adding time on the saponin synthesis and the hairy root growth of C. lanceolata. A significant decrease in major saponin (lancemaside of three kinds) content of hairy roots was observed with MeJA treatments. Contents of lancemaside A, B and E decreased about 15% more than non-treated hairy roots. In contrast, minor saponin (foetidissimoside A and aster saponin Hb) accumulation was about 15% higher than the non-treated hairy roots. These results suggest that MeJA treatment could be used in the production of teriterpene saponins.

• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.339-345
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• 2007

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing ${\gamma}-TMT$ gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding ${\gamma}-Tocopherol$ methyltransferase gene (${\gamma}-TMT$) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the $T_0\;and\;T_1$ generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. $T_1$ progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of $T_1$ progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.2
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• pp.248-252
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• 2015

This study was conducted to compare the growth, inorganic components, and proximate components of Codonopsis lanceolata grown in 10 regions of Korea for selecting superior species and breeding by crossing. Among the all tested lines, the shortest plant height (217.12 cm) was observed from the Ulleungdo region line (No. 4) while the longest (273.9 cm) was observed from Hwasun region line (No. 9). In addition, the lines of central and northern region (No. 1~No. 7) tend to have shorter plant height than those of southern region (No. 8~No. 9) except Jejudo region line (No. 10). Flowering tends to be late towards southern region, and lines in central and northern regions were started flowering about 2 weeks earlier than those in southern regions. However, the heaviest root weight was 13.1 g, found in only Jejudo line (No. 10) whereas there was no significant difference found in the other regions which have a range of 8.3~11.0 g. The inorganic components were varied in each line, however, proportion of macroelements, such as K, Ca, and P, was the largest for every line. Especially for Heongseong region line (No. 2), had larger proportion of macroelements than the others. There was a difference of proximate compositions of Codonopsis lanceolata, except the moisture content, among all regions, however, it was generally shown that the content of crude protein (1.31~3.76%) and crude fiber (2.18~3.12%) was the highest.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.274-274
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• 2017

Temperature has an adverse effect for potential growth and development of Codonopsis lanceolata. The present study examined the responses of growth, yield and mineral element contents under temperature variables in C. lanceolata. Plant height exhibited the best result as being 2 m or higher the open field culture, while the growth is the most restricted in the $30^{\circ}C$. The open field culture also had the largest number of leaves in the early stage of growth. The number of leaves and the height tended to be increased with the temperature of $20^{\circ}C$ and $25^{\circ}C$. The number of branches showed no significance as 32 branches and 26 branches in open field culture and $20^{\circ}C$, respectively. The smallest number of branches was observed in the $30^{\circ}C$ with 15 branches. The leaf color recorded the lowest value in the open field culture unlike the growth above the ground. But all other temperature had no significance with the leaf color value between 50.0 and 52.6. The elevated temperature induced the thinner and shorter diameter and length of root. However, the root diameter was not significantly changed with the change of 1.8 cm and 2.1 cm at open field culture, with $20^{\circ}C$ and $25^{\circ}C$, respectively. The fresh weights of the primary root and lateral roots were 6.3 g and 3.1 g, respectively, at the high temperature. The result showed the thickening of the lowest part under the ground as restricted. The fresh weights of the main root and lateral root were the highest on the open field culture, 20.6 g and 12.2 g, respectively. The contents of cellulose were the highest as 4.41% in the $20^{\circ}C$, followed by the open field culture, $25^{\circ}C$ and $30^{\circ}C$. The higher temperature reduced the contents of cellulose. Moreover, P showed higher ratio in the high temperature while Ca and Mg exhibited the higher ratio towards the low temperature. On the contrary, minor quantity (less than 1%) of mineral nutrients was observed towards all temperature variables.

• Korean Journal of Food Science and Technology
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• v.48 no.2
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• pp.104-110
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• 2016

In this study, the physicochemical and sensory characteristics of hot water extracts of Codonopsis lanceolata root skin (RS) and root flesh (RF) subjected to different heat treatments were analyzed to determine whether C. lanceolata RS can be used as a tea material. C. lanceolata RS and RF were roasted, hot-air dried (HDS and HDF, respectively), or steamed and hot-air dried (SHDS and SHDF, respectively). The heat-treated samples were extracted with hot water ($90^{\circ}C$) for 90 min. The hot water extracts of RS and RF had lower L-values and higher b-values than the other samples. The RS extracts had significantly higher polyphenol content and DPPH radical scavenging activities than RF samples. In the sensory evaluation, the color acceptability of the teas made from CS, HDS, and SHDS were found to be significantly different from those of the teas made from their respective flesh samples. The results suggest that the hot water extract of the C. lanceolata RS can be utilized as a tea material.

• Korean Journal of Plant Resources
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• v.5 no.1
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• pp.25-29
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• 1992

The proximate and amino acid composition of wild and cultivated Cynochum wilfordi, Codopsis lancolata and lxeris sonchifolia were analyzed. Cynochum wilfordi, had higher contents of sugar ascompared with Codonopsis lancolata and lxeris sonchifolia. Fats was 2-3 times higher in Cynochumwilordi then Codonopsis lancolata and lxeris sonchifolia. No significant difference in the contents wasfound between the open field root, spontanity and cultivated at 30'c in biotronroom at condonopsislanceolata. However, contents of protein was 12.8% in lxeris sonchifolia. Total amino acid were 1.5-1.8 times higher in lxeris sonchifolia. than Cynochum wilfordi and Codonopsis lancolata. The decresingorder of the amounts of amino acid infused from the Cynochum wilfordi samples was Glu, Arg, Asp,Leu, Pro, Lys, Codonopsis lancolata (Changdeuk) was Arg, Glu, Pro, Leu, Asp, Lys, Codonopsislanceilata(Chirisan) was Arg, Glu, Pro, Asp, Lys, Leu, Codonopsis lancolata(Yeanbeun) was Arg,Glu, Asp, Leu, Ser, Lys, and lxeris sonchifolia was Glu, Arg, Asp, Cys, Lys, Pro.