• Journal of Plant Biotechnology
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• 제37권1호
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• pp.102-109
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• 2010

Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in $T_2$ generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.

• Journal of the Institute of Electronics Engineers of Korea TE
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• 제37권1호
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• pp.35-41
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• 2000

This paper considers the consturction of 24/25 I-NRZI (Interleaved - Non Return to Zero Inverse) modulator designs for DVCR (Digital Video Cassette Recorder), and size of validity bit in order to store the amplitude value of square-wave and the standard data ( sine and cosine coefficients) at ROM Table that to acceptable the spectrum standard. The validity bit size of the standard data and the amplitude value of square-wave that to store at ROM Table are affected the size of pilot signal on the output spectrum, and the hardware size of modulator. At the designable 24/25 I-NRZI modulator, we simulated using random pattern (F0,F1,F2) that to verification the output data of the spectrum. Moreover, the resultant of the spectrum analysis, at the optimizing value, is 0.065 on the amplitude value of square-wave, and 3bit on the size of bit in order to store the standared data at ROM Table. In order to verify the hardware of designable 24/25 I-NRZI modulator, we perform to modeling of C-language firstly, and coding to Verilog HDL (Cadence Verilog XL) and synthesized using Synopsys (Library "Samsung KG75") tool as a base of spectrum results. In a foundation of this result, we are considered the size of hardware. In this paper, a considerable 24/25 I-NRZI modulator designable less than 10,000 gates as that is improved consturction as regards the path method of pre-coder etc, and able to application digital camcorders as now practical use.

• Korean Journal of Social Welfare
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• 제61권1호
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• pp.293-322
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• 2009

This study investigated the problems of remarried wife with alcoholic problem. Specifically, this study regarded the wife's alcoholic problem as the problems of dysfunctional interaction among family members rather than an individual problem. The study had three purposes as follows. First, what were the interactions sustaining the conflict of remarried family? Second, what were the characteristics of remarried family relating to family conflict? Third, what were the interventions of family therapy to resolve the conflicts of remarried family? The total family counseling was 9 sessions, which included individual, couple, and family counseling. The study used constant comparative analysis by using open coding method to find the factors relating interactions sustaining the conflict of remarried family, the characteristics of remarried family relating to family conflict, and the interventions of family therapy to resolve the conflicts of remarried family. Also, the study used Miles and Huberman's matrix and graphical network display to show the interaction of family members. The study found intriguing factors, counteracting factors, relationship level, and relational elements to wife's alcoholic problems among family members. The study revealed husband's rigid family rules, husband's execution of patriarchal authority, husband's pursuit of family identity, and husband's emphasis of union among family members as the characteristics of remarried family. On the other hand, the study found the reinforcement of spousal subsystem, the pursuit of change in communication pattern between husband and wife, the amelioration of rigid family rules, and the acceptance of differences among family members as the intervention of family therapy to resolve the conflicts of remarried family.

• Journal of the Korea Society of Computer and Information
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• 제12권1호
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• pp.161-168
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• 2007

Web attack uses structural, logical and coding error or web application rather than vulnerability to Web server itself. According to the Open Web Application Security Project (OWASP) published about ten types of the web application vulnerability to show the causes of hacking, the risk of hacking and the severity of damage are well known. The detection ability and response is important to deal with web hacking. Filtering methods like pattern matching and code modification are used for defense but these methods can not detect new types of attacks. Also though the security unit product like IDS or web application firewall can be used, these require a lot of money and efforts to operate and maintain, and security unit product is likely to generate false positive detection. In this research profiling method that attracts the structure of web application and the attributes of input parameters such as types and length is used, and by installing structural database of web application in advance it is possible that the lack of the validation of user input value check and the verification and attack detection is solved through using profiling identifier of database against illegal request. Integral security management system has been used in most institutes. Therefore even if additional unit security product is not applied, attacks against the web application will be able to be detected by showing the model, which the security monitoring log gathering agent of the integral security management system and the function of the detection of web application attack are combined.

• The Korean Journal of Malacology
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• 제25권3호
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• pp.223-230
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• 2009

Metallothioneins (MTs) play a key role in metallic homeostasis and detoxification in most living organisms. In an attempt to study the biological functions and significance of MT in a snail, we cloned and partially characterized the MT gene from the left-handed snail, Physa acutawhich has been regarded as a potential biomonitering species for fresh water. The complete cDNA sequence of PaMT cDNA was identified from the expressed sequence tag (EST) sequencing project of Physa acuta. The coding region of 180 bp gives 60 amino acid residues including the initiation methionine and termination codon. Clustering and phylogenic analysis of PaMT with other MT amino acid sequences show that it has some identities to Helix pomatia (60%), Arianta arbustorum (58%), Perna viridis (49%), Mytilus edulis (49%), Bathymodiolus azoricus (49%), Bathymodiolus azoricus (48%) and Bathymodiolus sp. FD-2002 (48%). Time dependent induction for PaMT from P. acuta exposed with cadmium (50 ppb) indicated that PaMT was induced at 4-8 hr after exposure. It remains to further develop PaMTas a potential biomarker for water contamination in fresh water.

• Journal of Animal Science and Technology
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• 제60권12호
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• pp.32.1-32.10
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• 2018

Background: Necdin (NDN), a member of the melanoma antigen family showing imprinted pattern of expression, has been implicated as causing Prader-Willi symptoms, and known to participate in cellular growth, cellular migration and differentiation. The region where NDN is located has been associated to QTLs affecting reproduction and early growth in cattle, but location and functional analysis of the molecular mechanisms have not been established. Methods: Here we report the sequence variation of the entire coding sequence from 72 samples of cattle, yak, buffalo, goat and sheep, and discuss its variation in Bovidae. Median-joining network analysis was used to analyze the variation found in the species. Synonymous and non-synonymous substitution rates were determined for the analysis of all the polymorphic sites. Phylogenetic analysis were carried out among the species of Bovidae to reconstruct their relationships. Results: From the phylogenetic analysis with the consensus sequences of the studied Bovidae species, we found that only 11 of the 26 nucleotide changes that differentiate them produced amino acid changes. All the SNPs found in the cattle breeds were novel and showed similar percentages of nucleotides with non-synonymous substitutions at the N-terminal, MHD and C-terminal (12.3, 12.8 and 12.5%, respectively), and were much higher than the percentage of synonymous substitutions (2.5, 2.6 and 4.9%, respectively). Three mutations in cattle and one in sheep, detected in heterozygous individuals were predicted to be deleterious. Additionally, the analysis of the biochemical characteristics in the most common form of the proteins in each species show very little difference in molecular weight, pI, net charge, instability index, aliphatic index and GRAVY (Table 4) in the Bovidae species, except for sheep, which had a higher molecular weight, instability index and GRAVY. Conclusions: There is sufficient variation in this gene within and among the studied species, and because NDN carry key functions in the organism, it can have effects in economically important traits in the production of these species. NDN sequence is phylogenetically informative in this group, thus we propose this gene as a phylogenetic marker to study the evolution and conservation in Bovidae.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제15권5호
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• pp.1666-1689
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• 2021

The red line of Permanent Basic Farmland is the most important part in the "three-line" demarcation of China's national territorial development plan. The scientific and reasonable delineation of the red line is a major strategic measure being taken by China to improve its ability to safeguard the practical interests of farmers and guarantee national food security. The delineation of Permanent Basic Farmland zoning (DPBFZ) is essentially a multi-objective optimization problem. However, the traditional method of demarcation does not take into account the synergistic development goals of conservation of cultivated land utilization, ecological conservation, or urban expansion. Therefore, this research introduces the idea of artificial immune optimization and proposes a multi-objective model of DPBFZ red line delineation based on a clone selection algorithm. This research proposes an objective functional system consisting of these three sub-objectives: optimal quality of cropland, spatially concentrated distribution, and stability of cropland. It also takes into consideration constraints such as the red line of ecological protection, topography, and space for major development projects. The mathematical formal expressions for the objectives and constraints are given in the paper, and a multi-objective optimal decision model with multiple constraints for the DPBFZ problem is constructed based on the clone selection algorithm. An antibody coding scheme was designed according to the spatial pattern of DPBFZ zoning. In addition, the antibody-antigen affinity function, the clone mechanism, and mutation strategy were constructed and improved to solve the DPBFZ problem with a spatial optimization feature. Finally, Tongxu County in Henan province was selected as the study area, and a controlled experiment was set up according to different target preferences. The results show that the model proposed in this paper is operational in the work of delineating DPBFZ. It not only avoids the adverse effects of subjective factors in the delineation process but also provides multiple scenarios DPBFZ layouts for decision makers by adjusting the weighting of the objective function.

• Design Convergence Study
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• 제16권6호
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• pp.169-184
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• 2017

There is a lot of needs that are not expressed as much as the expressed needs in familiar products and services that are used in daily life such as a smartphone. Finding the 'Inconveniences in familiar use' make it possible to create opportunities for value expanding in the existing products and service area. There are a lot of related works, which have studied the definition of hidden needs and the methods to find it. But, they are making it difficult to address the hidden needs in the cases of familiar use due to focus on the new product or service developing typically. In this study, we try to redefine the hidden needs in the daily familiarity and approach it in the new way to find out. Because of the users' unability to express what they want and the complexity of needs which can not be explained clearly, we can not approach it as the quantitative issue. For this reason, the basic data type selected as the user behavior data excluding all description is the screen-shot of the smartphone. We try to apply the integrated rules and patterns to the individual data using the qualitative coding techniques to overcome the limitations of qualitative analysis based on unstructured data. From this process, We can not only extract meaningful clues which can make to understand the hidden needs but also identify the possibility as a way to discover hidden needs through the review of relevance to actual market trends. The process of finding hidden needs is not easy to systemize in itself, but we expect the possibility to be conducted a reference frame for finding hidden needs of other further studies.

• Journal of Life Science
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• 제16권1호
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• pp.131-135
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• 2006

Oligopeptide is known to be an essential nitrogen nutrient for bacterial growth. Oligopeptide can be transported into cytoplasm by a specific transport system, Opp system. Opp system is composed of five proteins, which are transcribed by an operon. These are responsible for oligopeptide binding protein (OppA), permease (OppB and OppC) and energy generation system (OppD and OppF), respectively. Previously, we isolated the opp operon from Vibrio fluvialis and constructed the oppA mutant by allelic exchange method. In this study, we investigated the growth pattern and biofilm production under the different growth condition. When the cells were cultivated using brain heart infusion(BHI) medium, the wild type was faster than the mutant in growth during the exponential phase. However, it showed that the growth pattern of two strains in M9 medium is very similar. The growth of wild type showed better than that of the mutant grown at pH 8. At pH 7, there was no an obvious difference in growth. After 5 mM $H_2O_2$ was treated to the cells $(OD_{600}=1.2)$, the cell survival was examined. The oppA mutation did not affect in survivability. In the presence of $10{\mu}g/ml$ polymyxin B, the biofilm production of the oppA mutant was higher than that of the wild type.

• Journal of Korean Society of Forest Science
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• 제80권2호
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• pp.151-161
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• 1991

I tried to specify the taxa of Fagaceae in Korea by the character of their pollen grains. The light microscope(LM) and the Scanning electron microscope(SEM) were used to examine the pollen grains of 19 taxa, 5 genera. The result are as follows. 1. The pollen of Fagaceae in Korea could be grouped into four types and 4 subtypes. 1) Fagus type 2) Castanea type 3) Lithocarpus, Castanopsis type 4) Quercus type (1) Cyclobalanopsis subtype (2) Prinus subtype (3) Dentatae subtype (4) Cerris subtype. 2. The morphology of the granula on the pollen of Quercus was closely related to the differantiation of the shape of the cup scales. 1) The uniformity of branching granula on the pollen grain surface corresponds to the morphological features of the concentric arrangement of cup scales. 2) The morphological features of the pollen grain surface intermingled with large or small granula, simple-granula and tuber granula which have small points of circular prominence, corresponded to those of short cup scales. 3) The morphological features of the polllen grain surface intermingled with large or small granula, simple-granula and tuber granula with an apex of amoeba type corresponded to those of Q. dentata Thunb, with thin, fine and long cup scales. 4) The morphological features of the pollen grain surface intermingled with large of small granula of with only simple-granula, corresponded to those of Q. acutissima carr. with thick, fine and long cup scales. 3. The result of cluster analysis by coding the sculpture pattern of the pollen grain surface, the existence and nonexistence of surface perforate, the grain size and granula type were coincident with the system of classification of plants and showed an intimated relationship even under th level of species.