The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
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Molecular cloning and expression pattern of Metallothionein Gene from the left-handed shell, Physa acuta

왼돌아물달팽이 (Physa acuta) 의 Metallothionein 유전자 클로닝 및 발현양상

  • Jo, Yong-Hun (Department of Applied Biology, College of Agriculture and Life Science, Chonnam National University) ;
  • Baek, Moon-Ki (Department of Parasitology, Inje University College of Medicine) ;
  • Kang, Se-Won (Department of Parasitology, Inje University College of Medicine) ;
  • Lee, Jae-Bong (Department of Parasitology, Inje University College of Medicine) ;
  • Byun, In-Seon (Dept. of Immuology SoonChunHyang University College of Medicine) ;
  • Choi, Sang-Haeng (Industrial biotechnology & bioenergy research center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chae, Sung-Hwa (Research Institute of GnC BIO Co., LTD.) ;
  • Kang, Jung-Ha (Biotechnology Research Division, NFRDI) ;
  • Han, Yeon-Soo (Department of Applied Biology, College of Agriculture and Life Science, Chonnam National University) ;
  • Park, Hong-Seog (Industrial biotechnology & bioenergy research center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Yong-Seok (Department of Parasitology, Inje University College of Medicine)
  • 조용훈 (전남대학교 농과대학 식물생명공학부) ;
  • 백문기 (인제대학교 의과대학 기생충학교실) ;
  • 강세원 (인제대학교 의과대학 기생충학교실) ;
  • 이재봉 (인제대학교 의과대학 기생충학교실) ;
  • 변인선 (순천향대학교 의과대학 면역학교실) ;
  • 최상행 (한국생명공학연구원 바이오화학에너지센터) ;
  • 채성화 ((주)지앤시바이오) ;
  • 강정하 (국립수산과학원 생명공학과) ;
  • 한연수 (전남대학교 농과대학 식물생명공학부) ;
  • 박홍석 (한국생명공학연구원 바이오화학에너지센터) ;
  • 이용석 (인제대학교 의과대학 기생충학교실)
  • Received : 2009.08.25
  • Accepted : 2009.10.20
  • Published : 2009.12.31
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Abstract

Metallothioneins (MTs) play a key role in metallic homeostasis and detoxification in most living organisms. In an attempt to study the biological functions and significance of MT in a snail, we cloned and partially characterized the MT gene from the left-handed snail, Physa acutawhich has been regarded as a potential biomonitering species for fresh water. The complete cDNA sequence of PaMT cDNA was identified from the expressed sequence tag (EST) sequencing project of Physa acuta. The coding region of 180 bp gives 60 amino acid residues including the initiation methionine and termination codon. Clustering and phylogenic analysis of PaMT with other MT amino acid sequences show that it has some identities to Helix pomatia (60%), Arianta arbustorum (58%), Perna viridis (49%), Mytilus edulis (49%), Bathymodiolus azoricus (49%), Bathymodiolus azoricus (48%) and Bathymodiolus sp. FD-2002 (48%). Time dependent induction for PaMT from P. acuta exposed with cadmium (50 ppb) indicated that PaMT was induced at 4-8 hr after exposure. It remains to further develop PaMTas a potential biomarker for water contamination in fresh water.

Keywords

References

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