• Journal of Microbiology and Biotechnology
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• v.19 no.5
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• pp.439-446
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• 2009

The biosynthesis study of antibiotics saframycin (SFM) in Streptomyces lavendulae and safracin (SAC) in Pseudomonas fluorescens demonstrated that 3-hydroxy-S-methyl-O-methyltyrosine (3hSmOmTyr), a nonproteinogenic amino acid, is the precursor of the tetrahydroisoquinoline molecular core. In the biosynthetic gene cluster of SAC/SFM, sacD/sfmD encodes a protein with high homology to each other but no sequence similarity to other known enzymes; sacF/sfmM2 and sacG/sfmM3 encode methyltransferases for C-methylation and O-methylation; and sacE/sfinF encodes a small protein with significant sequence similarity to the MbtH-like proteins, which are frequently found in the biosynthetic pathways of non ribosomal peptide antibiotics and siderophores. To address their function, the biosynthetic cassette of 3h5mOmTyr was heterologously expressed in S. coelicolor and P. putida, and an in-frame deletion and complementation in trans were carried out. The results revealed that (i) SfmD catalyzes the hydroxylation of aromatic rings; (ii) sacD/sacF/sacG in the SAC gene cluster and sfmD/sfmM2/sfmM3 in the SFM cluster are sufficient for the biosynthesis of 3h5mOmTyr; and (iii) the mbtH-like gene is not required for the biosynthesis of the 3h5mOmTyr precursor.

• The Journal of the Korea Contents Association
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• v.10 no.2
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• pp.14-24
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• 2010

Because WSNs operate with limited resources of sensor nodes, its life is extended by cluster-based routing methods. In this study, we use data on direction, distance, density and residual energy in order to maximize the energy efficiency of cluster-based routing methods. Through this study, we expect to minimize the frequency of isolated nodes when selecting a new cluster head autonomously using information on the direction of the upper cluster head, and to reduce energy consumption by switching sensor nodes, which are included in both of the new cluster and the previous cluster and thus do not need to update information, into the sleep mode and updating information only for newly included sensor nodes at the setup phase using distance data. Furthermore, we enhance overall network efficiency by implementing secure and energy-efficient communication through key management robust against internal and external attacks in cluster-based routing techniques. This study suggests the modified cluster head selection scheme which uses the conserved energy in the steady-state phase by reducing unnecessary communications of unchanged nodes between selected cluster head and previous cluster head in the setup phase, and thus prolongs the network lifetime and provides secure and equal opportunity for being cluster head.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.10
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• pp.3972-3988
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• 2020

Existing methods always rely on statistical features to extract local words for microblog user geolocation. There are many non-local words in extracted words, which makes geolocation accuracy lower. Considering the statistical and semantic features of local words, this paper proposes a microblog user geolocation method by extracting local words based on word clustering and wrapper feature selection. First, ordinary words without positional indications are initially filtered based on statistical features. Second, a word clustering algorithm based on word vectors is proposed. The remaining semantically similar words are clustered together based on the distance of word vectors with semantic meanings. Next, a wrapper feature selection algorithm based on sequential backward subset search is proposed. The cluster subset with the best geolocation effect is selected. Words in selected cluster subset are extracted as local words. Finally, the Naive Bayes classifier is trained based on local words to geolocate the microblog user. The proposed method is validated based on two different types of microblog data - Twitter and Weibo. The results show that the proposed method outperforms existing two typical methods based on statistical features in terms of accuracy, precision, recall, and F1-score.

• Proceedings of the Materials Research Society of Korea Conference
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• 1994.11a
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• pp.1-1
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• 1994

;The electronic state calculations for various types of ceramic materials have beell performed by the use of $DV-X\;{\alpha}$ cluster method. The molecular orbital levels and wave functions for model clusters have been computed to study the electronic properties ami chemical bonding of the ceramics. For ${\beta}-sialon(Si_{6-z}Al_zO_zN_{8-z})$ which is a high temperature structural material based on ${\beta}-Si_3N_4$, we have made model cluster calculations to estimate the strength of chemical bonding between atoms by the Mulliken population analysis. It is found that the covalent bonding between Si and N atoms is very strong in pure ${\beta}-Si_3N_4$, but the covalency around solute atom is considerably weakened when Si atom is substituted by AI. This tendency is enhanced by an additional substitution of oxygen atom for N. The result calculated can well explain the experimental data of changes in mechanical properties such as the reductions of Young's modulus and Vickers hardness with increment of z-value in ${\beta}-sialon$. Various model clusters for transition metal oxides which show many interesting physical and chemical properties have also been calculated. High-valent perovskite-type iron oxides EMFe0_3E(M=Ca and Sr) possess very interesting magnetic and chemical properties. In these oxides, iron exists as $Fe^{4+}$ state, but the experimental measurement of Mossba~er effect suggests that disproportionation $2Fe^{4+}=Fe^{3+}+Fe^{5+}$ takes place for $CaFe0_3$ at low temperatures. The model cluster calculations for these compounds indicated the existence of considerably strong covalent bonding of Fe-O. The calculations of hyperfine interaction at iron neucleus show very good agreement with the experimental Mossbauer measurements. The result calculated also implies that the disproportionation reaction is strongly possible by assuming the quenching of breathing phonon mode at low temperatures.tures.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.2
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• pp.30.3-31
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• 2015

We present a kinematic analysis of 172 likely member galaxies of the Ursa Major Cluster. In order to understand the dynamical state of the cluster, we investigate the correlation of the cluster morphology with rotation, the velocity dispersion profile, and the rotation amplitude parallel to the global rotation direction. Both the minor axis and the rotation are very well-aligned with the global rotation axis in the outer region at half radius (> 0.5 $R_{max}$), but not in the inner region. The cluster exhibits low velocity dispersion and rotation amplitude profiles in the inner region, but higher in the outer. Both profiles exhibit outwardly increasing trends, suggesting an inside-out transfer of angular momentum of dark matter via violent relaxation, as revealed by a recent off-axis major-merging simulation. From Dressler-Schectman plots in the plane of galactic positions, and velocity versus position angle of galaxy, we are able to divide the Ursa Major Cluster into two substructures: Ursa Major South (UMS) and Ursa Major North (UMN). We derive a mass of $3.2{\times}10^{14}M_{\odot}$ for the cluster through the two-body analysis by the timing argument with the distance information (37 for UMN and 36 for UMS) and the spin parameter of ${\lambda}=0.049$. The two substructures appear to have passed each other 4.4 Gyr ago and are moving away to the maximum separation.

• The Journal of the Korea Contents Association
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• v.9 no.1
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• pp.167-176
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• 2009

Traditional cluster-based routing method is a representative method for increasing the energy efficiencies. In these cluster-based routing methods, the selected cluster head collect/aggregate the information and send the aggregated information to the base station. But they have to solve the unnecessary energy dissipation of frequent information exchange between the cluster head and whole member nodes in cluster. In this paper, we minimize the frequency of the information exchange for reducing the unnecessary transmit/receive frequencies as calculate the overlapped area or number of overlapped member nodes between the selected cluster head and previous cluster head in the setup phase. So, we propose the modified cluster selection protocol method that optimizes the energy dissipation in the setup phase and reuses the saved energy in the steady-state phase efficiently that prolongs the whole wireless sensor network lifetime by uniformly selecting the cluster head.

• Journal of Technology Innovation
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• v.14 no.2
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• pp.1-27
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• 2006

This paper rejuvenates the existing discussion on the importance of cluster approach to industry development strategies. Current evidences suggest that the shape of economic policy and practice is changing significantly around the world. Governments continually search for new tools and policy formulas to improve economic performance and create economic prosperity for all citizens. In this context a more proactive and strategic role for government in support of the cluster-based economic development model has emerged. This paper uses Singapore's petrochemical industry as an example to study the cluster approach to industry development. In doing so, there is much optimism to the importance of state and its institutions to play a significant role on industry development. Nevertheless, the study also raises doubts on whether the cluster-based strategy is due to the concept itself or due to other important factors.

• Bulletin of the Korean Chemical Society
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• v.14 no.1
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• pp.63-71
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• 1993

By use of cluster orbitals, analytic solutions of finite face-centered cubic clusters are obtained. Taking interactions between up to the second nearest neighbors into account, the forms of all the elements of the Hamiltonian matrix are found explicitly within Huckel approximation. By adopting $D_{2k}$ point group to the cluster, the matrix is simplified. We assume that the cluster orbitals can mix together only when their state indices are indentical. It is then possible to calculate various physical properties of face-centered cubic metal clusters and example are shown for palladium clusters. The results show that density of states and projected density of states are similar, qualitatively, with those obtained by extended Huckel calculation.

• East Asian Journal of Business Economics (EAJBE)
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• v.7 no.2
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• pp.21-30
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• 2019

Purpose - In this work, we categorize the 21 shopping items which foreign tourists purchase in South Korea and monitor the level of dissimilarity (or similarity) between each item by utilizing distance matrix, and both hierarchical and k-means cluster analyses, respectively, based on several purpose of visit attributes in 2017. In addition, multidimensional scaling (MDS) method is applied for mining visual appearance of proximities among shopping items based on purpose of visit attributes. Research design and methodology - This study is carried out in 2017 by Ministry of Culture, Sports and Tourism and conduct a face-to-face survey of foreign tourists from 20 countries who purchase shopping items in South Korea. CLUSTER, PROXIMITIES and ALSCAL modules in IBM SPSS 23.0 are used to perform this work. Results - We ascertain that 21 shopping items can be classified into five similar groups which have homogeneous traits by going through two-step cluster analysis. We can position homogeneous places of cluster and shopping items joining each cluster. Conclusions - We can relatively assess patterns and characteristics of each shopping item, come by useful information in activating shopping tour based on the actual state of recognition of foreign tourists and practically apply to each tourism industry on underlying results.

• Convergence Security Journal
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• v.8 no.1
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• pp.91-100
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• 2008

The previous works in sensor networks security have focused on the aspect of confidentiality, authentication and integrity based on cryptographic primitives. There has been no prior work to assess the survivability in systematic way. Accordingly, this paper presents a survivability model of wireless sensor networks using software rejuvenation for dual adaptive cluster head. The survivability model has state transition to reflect status of real wireless sensor networks. In this paper, we only focus on a survivability model which is capable of describing cluster head compromise in the networks and able to switch over the redundant cluster head in order to increase the survivability of that cluster. Second, this paper presents how to enhance the survivability of sensor networks using software rejuvenation methodology for dual cluster head in wireless sensor network. We model and analyze each cluster as a stochastic process based on Semi Markov Process (SMP) and Discrete Time Markov Chain (DTMC). The proof of example scenarios and numerical analysis shows the feasibility of our approach.