• 전기학회논문지
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• 제58권1호
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• pp.28-34
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• 2009

In this study firstly we survey the calculation method and the characteristics of the way of estimating CBL(Customer BaseLine Load) that is important calculation tool for DRP internationally. Also we analyze the power consumption pattern using the 15 minutes load profiles of about 120,000 customers in domestic. Based on this pattern, we provide the CBL calculation method that can be utilized in DRP to save the cost, and analyze the accuracy of the CBL calculation proposed in this paper through the simulation.

• Animal cells and systems
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• 제5권3호
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• pp.195-198
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• 2001

Hepatitis B virus (HBV) polymerase, which possesses the activities of terminal binding, DNA polymerase, reverse transcriptase and RNaseH, has been shown to accomplish viral DNA replication through a pregenomic intermediate. Because the HBV polymerase has not been purified, the expression of HBV polymerase was examined in an E. coli expression system that is under the regulation of arabinose operon. The expressed individual domain containing terminal binding protein, polymerase, or RNaseH turned out to be insoluble. The activities of those domains were not able to be recovered by denaturation and renaturation using urea or guanidine-HCI. The expressed reverse transcriptase containing the polymerase and RNaseH domains became extensively degraded, whereas the proteolysis was reduced in a Ion- mutant. These results indicate that Lon protease proteolyzes the HBV reverse transcriptase expressed in E. coli.

• 한국정보처리학회논문지
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• 제6권3호
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• pp.742-749
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• 1999

This paper proposes the mechanism of cell buffering in the output buffer of ATM switch for the traffic with the different QOS in the ATM environment. The proposed mechanism, VQS(Variable Queue Sharing) can minimize the cell loss ratio(CLR) of bursty traffic through the sharing of CBR queue, VBR queue, ABR queue to maximize the utilization of queue resource. To evaluate VQS performance, we make simulator using Visual Slam 2.0. We compare the CLP and cell average delay of VQS and HOL, QLT_HOL using the bursty traffic patterns.

• 한국컴퓨터산업학회논문지
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• 제3권1호
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• pp.19-26
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• 2002

다양한 QoS를 갖는 트래픽을 위해, ATM 스위치의 출력버퍼에 저장되는 셀의 큐 구조를 제안하였다. 제안한 큐 구조는 기존의 고정길이 큐 구조의 문제점인 버스트 트래픽으로 인한 큐 넘침의 문제점을 개선하기 위해 실시간 트래픽과 비실시간 트래픽을 묶어 큐를 공유하여 큐 이용률을 최대화시키는 구조를 기반으로 하고 있다. 즉, CBR 트래픽과 VBR-nrt를 묶고, VBR-rt와 ABR 트래픽을 묶어 버스트 트래픽 유입으로 인한 셀 손실률을 최소화시킬 수 있는 특성을 제공한다. 제안 큐 구조의 성능을 평가하기 위해 버스트 특성이 강한 트래픽 패턴을 이용하여 기존의 큐 구조와 셀 손실률 및 평균 지연률을 비교하였다.

• Journal of Dairy Science and Biotechnology
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• 제40권2호
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• pp.86-91
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• 2022

Chryseobacterium mulctrae KACC 21234^T is a novel species isolated from raw bovine milk. Psychrotrophic bacteria are considered contaminants and are hypothesized to originate from the environment. In this investigation, the C. mulctrae KACC 21234^T genome was determined to be 4,868,651 bp long and assembled into four contigs with a G+C ratio of 33.8%. In silico genomic analyses revealed the presence of genes encoding proteases (endopeptidase Clp, oligopeptidase b, carboxypeptidase) and lipases (phospholipase A(2), phospholipase C, acylglycerol lipase) that can catalyze the degradation of the proteins and lipids in milk, causing its quality to deteriorate. Additionally, antimicrobial resistance and putative bacteriocin genes were detected, potentially intensifying the pathogenicity of the strain. The genomic evidence presented highlights the need for improved screening protocols to minimize the potential contamination of milk by proteolytic and lipolytic psychrotrophic bacteria.

• 생명과학회지
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• 제22권4호
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• pp.552-558
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• 2012

사스(Severe acute respiratory syndrome, SARS)는 사람의 신종 폐렴인 중증 급성 호흡기 질환으로 신종 코로나바이러스, SARS-CoV에 의해 유발된다. 3CL protease는 SARS-CoV의 복제, 전사 및 단백질 합성을 조절하는 복제효소 복합단백질의 프로세싱에 결정적인 역할을 담당하는 중요한 효소이다. 따라서, 이 효소를 저해함으로써 SARS-CoV의 증식을 억제하고 사스의 증폭 및 확산을 막을 수 있다. SARS-3CL protease의 활성 저해물질의 탐색은 사스의 치료제 개발에 중요한 목표 중의 하나로 인식되고 있으며 이를 위해서는 활성형 SARS-3CL protease의 대량 생산이 필요하다. 본 연구에서는 활성형 SARS-3CL protease를 대량 생산하기 위하여 여러 가지 발현 벡터 및 단백질 발현 방법 등을 검토하였다. 그 결과, pET29a/3CLP 발현 벡터를 이용한 무세포 단백질 합성법이 SARS-3CL protease 생산에 최적 조건인 것으로 확인되었다. 또한 발현된 효소를 완전히 정제하여 그 특성을 분석한 결과, 본 효소는 무세포 단백질 합성계에서 전구체로 합성됨과 동시에 자가분해됨으로써 모든 단백질이 활성형인 성숙체 단백질로 전환되어 간단히 활성형 SARS-3CL protease 효소를 생산할 수 있음을 확인하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권5호
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• pp.2765-2769
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• 2013

Background: A close association between patterns identified by magnifying narrow-band imaging (M-NBI) and histological type has been described. M-NBI patterns were also recently reported to be related to the mucin phenotype; however, detials remain unclear. Materials and Methods: We investigated the cellular differentiation of gastric cancer lesions, along with their mucosal distribution observed by M-NBI. Ninety-seven depressed-type early gastric cancer lesions (74 differentiated and 23 undifferentiated adenocarcinomas) were visualized by M-NBI. Findings were divided into 4 patterns based on abnormal microvascular architecture: a chain loop pattern (CLP), a fine network pattern (FNP), a corkscrew pattern (CSP), and an unclassified pattern. Mucin phenotypes were judged as gastric (G-type), intestinal (I-type), mixed gastric and intestinal (M-type), and null (N-type) based on 4 markers (MAC5AC, MUC6, MUC2, and CD10). The relationship of each pattern of microvascular architecture with organoid differentiation indicated by cancer cell differentiation and its distribution in each histological type of early gastric cancer was investigated. Results: All CLP and FNP lesions were differentiated. The cancer cell distribution showed organoid differentiation in 84.2% (16/19) and 61.1% (22/36) of the two types of lesions, respectively, and there was a significant difference from the unclassified pattern with organoid differentiation (p<0.001). Almost all (94.7%; 18/19) CSP lesions were undifferentiated, and organoid differentiation was observed in 72.2% (13/18). There was a significant difference from the unclassified pattern with organoid differentiation (p<0.05). Conclusions: Cellular differentiation and distribution are associated with microvascular architecture observed by M-NBI.

• 대한구순구개열학회지
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• 제7권1호
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• pp.35-46
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• 2004

The frequency of abnormality at birth is average 1-1.5%, and of these, cleft lip & palate is known to be the most frequent congenital abnormality, Cleft is considered to be due to multi-factorial heredity correlated with genetic and environmental factors, Cleft patients require the collaborative treatment with several medical departments, Clinics for Maxillo-Oral Disorders of Tohoku University Dental Hospital performs the total managements related to such as occlusion and language for the patients with congenital maxilla-facial abnormality, This study examined the patients with cleft lip and/or palate who came to the Clinics for Maxilla-Oral Disorders of Tohoku University Dental Hospital for the past 17 years from Jan. 1987 to Dec, 2002, and had the results as follows, 1. Annual mean number of patients The annual mean number of the patients for 17 years from Jan, 1987 to Dec, 2002 was 91 patients, ranging from 63 minimum to 116 maximum, 2, Gender and types of cleft There were 747(51%) males and 709(49%) females, with a male to female ratio 1,05:1. CLP was the most frequent cleft type as shown in 616 patients, and other patients manifested different complaints such as CL, CP, SMCP and MC in order. 3. The laterality in cleft type The lip cleft was frequently expressed orderly on left, right and both sides of CL patients while orderly being shown on left, both and right sides of CLP patients. Accordingly, lip cleft was most commonly found on the left side. 4. Address at first visit Of 1,456 subjects, 850(58.4%) patients were residing in Miyagi Prefecture, where this hospital is located. 5. Age at first visit 615(42.2%) patients came to the hospital at their age younger than 1 year old, comprising 282(19.4%) patients age younger than 2 months old and 333(22.9%) patients age between 2 month old and 1 year old. 6. Mother's age at birth For the mother's age at birth, 526(39.9%) patients were at the age of 25 to 30 years old, and 17(1.3%) patients were over 40 years old. 7. Birth weight 34.3%(443 patients) had a birth weight of 2500-3000gm and 56.0%(724 patients) had a 3000-4000gm. It was also found that 7.9%(102 patients) had a birth weight of less than 2500gm. 8. Familial expression The frequency of familial expression was 6.5%(94 patients).

• 한국전산구조공학회논문집
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• 제34권3호
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• pp.167-174
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• 2021

최근 국내 원자력발전소의 격납건물 벽체와 Containment Liner Plate(CLP) 사이에서 다양한 크기의 공극이 발견됨에 따라 원전 격납건물의 보수를 위해 내부 공극의 분포와 크기를 정밀하게 평가할 수 있는 진단기법의 개발이 요구되고 있다. 이에 따라 이 연구에서는 격납건물 벽체에서의 탄성파 전파거동을 계산하는 2차원 유한요소해석 기법을 제시한다. 격납건물 벽체를 기반으로 해석영역을 구성하고 경계면에서의 반사파를 제거하기 위해 수치적 파동흡수 경계층인 perfectly matched layer를 도입하였다. Galerkin 기반 혼합유한요소법을 이용해 2차원 유한영역에서 탄성파 파동방정식의 해를 구하여 충격하중에 대한 격납건물 벽체의 변위와 응력을 계산하였다. 제시한 수치적 기법을 이용하여 격납건물 콘크리트 벽체의 CLP 부착 유무와 공동의 위치 및 크기 변화에 따른 탄성파 전파거동을 살펴보았다. 이 연구의 결과는 원전 격납건물 내부의 공동을 진단하는 탄성파 전체파형 역해석 기법 개발에 활용될 수 있다.

• Wind and Structures
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• 제24권5호
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• pp.447-464
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• 2017

The flutter instability is one of the most important themes need to be carefully investigated in the design of long-span bridges. This study takes the central-slotted ideal thin flat plate as an object, and examines the characteristics of unsteady surface pressures of stationary and vibrating cross sections based on computational fluid dynamics (CFD) simulations. The flutter derivatives are extracted from the surface pressure distribution and the critical flutter wind speed of a long span suspension bridge is then calculated. The influences of angle of attack and the slot ratio on the flutter performance of central-slotted plate are investigated. The results show that the critical flutter wind speed reduces with increase in angle of attack. At lower angles of attack where the plate shows the characteristics of a streamlined cross-section, the existence of central slot can improve the critical flutter wind speed. On the other hand, at larger angles of attack, where the plate becomes a bluff body, the existence of central slot further reduces the flutter performance.