• KSBB Journal
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• 제31권1호
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• pp.79-84
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• 2016

Biofuel has been considered as promising renewable energy to solve various problems that result from increasing usage of fossil fuels since the early 20th century. In terms of chemical and physical properties as fuel, biobutanol has more merits than bioethanol. It could replace gasoline for transportation and industrial demand is increasing significantly. Production of butanol can be achieved by chemical synthesis or by microbial fermentation. The water hyacinth, an aquatic macrophyte, originated from tropical South America but is currently distributed all over the world. Water hyacinth has excellent water purification capacity and it can be utilized as animal feed, organic fertilizer, and biomass feedstock. However, it can cause problems in the rivers and lakes due to its rapid growth and dense mats formation. In this study, the potential of water hyacinth was evaluated as a lignocellulosic biomass feedstock in biobutanol fermentation by using Clostridium beijerinckii. Water hyacinth was converted to water hyacinth hydrolysate medium through pretreatment and saccharification. It was found that productivity of water hyacinth hydrolysate medium on biobutanol production was comparable to general medium.

• KSBB Journal
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• 제20권6호
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• pp.401-407
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• 2005

본 연구는 C. beijerinckii KCTC 1785의 배양배지(RCM)에서 수소생산을 위한 최적화 조건을 탐색하고 수소생산을 위한 배지성분을 최적화하였다. 수소생산을 위한 최적 초기 pH와 발효온도는 각각 7.0과 $35^{\circ}C$이었고, 교반은 수소생산을 가속화 시켰다. C. beijerinckii KCTC 1785는 6%(w/v)의 glucose 농도까지 성장할 수 있지만 4%의 glucose 농도에서 가장 많은 수소를 생산하였으며, 이 때 바이오가스 중 수소 함량은 37%(v/v)이었다. 그러나 배지내 잔여 glucose 양을 고려할 때 수소생산을 위한 최적 glucose 농도는 1%이었다. 발효가 진행되는 동안 수소가 생산됨과 동시에 acetic acid와 butyric acid가 동시에 생성되었으며, acetic acid와 butyric acid가 각각 5,000 mg/L과 3,000 mg/L 이상의 농도에서 수소생산을 저해하였다. 또한 발효조의 pH를 5.5로 계속 유지하였을 경우 15시간 동안 0.5% glucose로부터 1,728 mL의 수소를 생산하였으며 이 때 수소의 생산수율은 1.23 mol $H_2/mol$ glucose이었다. C. beijerinckii KCTC 1785의 성장에 있어서 yeast extract 또는 tryptose는 반드시 필요한 배지의 성분이었다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2008년도 춘계학술대회 논문집
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• pp.235-238
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• 2008

We examined butanol fermentation by Clostridium beijerinckii NCIMB 8052 using various hydrolyzates obtained from rice bran which is one of the most abundant agricultural by-products in Korea and Japan. In order to increase the amount of fermentable sugars in the hydrolyzates of rice bran, various hydrolysis procedures were applied. Total eight different hydrolyzates were prepared using rice bran (RB) and defatted rice bran (DRB) with enzyme or acid treatment and both. Each hydrolyzate was evaluated in terms of total sugar concentration and butanol production after fermentation by C. beijerinckii NCIMB 8052. Acid treatment yielded more sugar than enzyme treatment and combined treatment with enzyme and acid yielded even more sugars as compared to single treatment with enzyme or acid. As a result, the highest sugar concentration (33 g/L) was observed from the hydrolyzate from DRB (100 g/L) with combined treatment using enzyme and acid. Prior to perform fermentation of the hydrolyzates, we examined the effect of P2 solution containing yeast extract, buffer, minerals, and vitamins on production of butanol during the fermentation. Fermentation of the hydrolyzates with or without additionof P2 was performed using C. beijerinckii NCIMB 8052 in a 1 L anaerobic bioreactor. Although the hydrolyzates RB were able to support growth and butanol production, addition of P2 solution into the hydrolyzates significantly improved cell growth and butanol production. Highest butanol production (12.24 g/L) was observed from the hydrolyzate of DRB with acid and enzyme treatment after supplementation of P2 solution.

• Journal of Microbiology and Biotechnology
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• 제19권5호
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• pp.482-490
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• 2009

We examined butanol fermentation by Clostridium beijerinckii NCIMB 8052 using various hydrolyzates obtained from rice bran, which is one of the most abundant agricultural by-products in Korea and Japan. In order to increase the amount of fermentable sugars in the hydrolyzates of rice bran, various hydrolysis procedures were applied. Eight different hydrolyzates were prepared using rice bran (RB) and defatted rice bran (DRB) with enzyme or acid treatment or both. Each hydrolyzate was evaluated in terms of total sugar concentration and butanol production after fermentation by C. beijerinckii NCIMB 8052. Acid treatment yielded more sugar than enzyme treatment, and combined treatment with enzyme and acid yielded even more sugars as compared with single treatment with enzyme or acid. As a result, the highest sugar concentration (33 g/l) was observed from the hydrolyzate from DRB (100 g/l) with combined treatment using enzyme and acid. Prior to fermentation of the hydrolyzates, we examined the effect of P2 solution containing yeast extract, buffer, minerals, and vitamins on production of butanol during the fermentation. Fermentation of the hydrolyzates with or without addition of P2 was performed using C. beijerinckii NCIMB 8052 in a 1-1 anaerobic bioreactor. Although the RB hydrolyzates were able to support growth and butanol production, addition of P2 solution into the hydrolyzates significantly improved cell growth and butanol production. The highest butanol production (12.24 g/l) was observed from the hydrolyzate of DRB with acid and enzyme treatment after supplementation of P2 solution.

• 공업화학
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• 제18권2호
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• pp.162-167
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• 2007

본 연구에서는 Clostridium beijerinckii Donker 1926을 이용한 연속식 혐기성 소화공정에서 수소 생산과 동력학적 특성을 고찰하였다. 기질은 glucose를 사용하였고, 0.5, 0.25, 0.125일의 체류시간 (hydraulic retention time, HRT)에서 실험이 이루어졌으며, 모든 HRT의 조건에서 탄산화물은 99% 이상의 제거효율를 나타내었다. 체류시간이 짧을수록, COD 제거율은 낮은 반면에, 전체 가스 중에서 수소 가스 함량과 수소 발생량이 높게 나타났다. 또한, 정상상태에서, 증식 수율과 수소가스 생성 수율은 각각 0.27 g-VSS/g- glucose, 0.26 L/g-glucose로 나타났다. 본 실험에 사용된 균주를 glucose와 같이 당 성분이 함유된 폐수처리에 적용하면 수소를 생산할 수 있으며, 이러한 실험 결과들을 잘 활용하면 대체에너지로서, 실제적인 수소가스 생산 시스템에 적용할 수 있을 것이다.

• Journal of Microbiology and Biotechnology
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• 제23권8호
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• pp.1092-1098
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• 2013

Cassava constitutes an abundant substrate in tropical regions. The production of butanol in ABE fermentation by Clostridium beijerinckii BA101 using cassava flour (CF) was scaled-up to bioreactor level (5 L). Optimized fermentation conditions were applied; that is, $40^{\circ}C$, 60 g/l CF, and enzymatic pretreatment of the substrate. The batch fermentation profile presented an acidogenic phase for the first 24 h and a solventogenic phase afterwards. An average of 37.01 g/l ABE was produced after 83 h, with a productivity of 0.446 g/l/h. Butanol production was 25.71 g/l with a productivity of 0.310 g/l/h, high or similar to analogous batch processes described for other substrates. Solvent separation by different combinations of fractioned and azeotropic distillation and liquid-liquid separation were assessed to evaluate energetic and economic costs in downstream processing. Results suggest that the use of cassava as a substrate in ABE fermentation could be a cost-effective way of producing butanol in tropical regions.

• 공업화학
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• 제22권5호
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• pp.447-452
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• 2011

석유자원의 고갈이 에너지 및 화학원료물질로 재생 가능한 바이오매스의 이용성을 증가시키고 있다. 본 총설에서는 바이오에너지 및 바이오화학원료인 C4-C6 생산에 관해 논하고자 한다. 주요한 C4 물질인 n-butanol과 n-butyric acid를 다량 생산하는 미생물은 Clostridium tyrobutyricum, Clostridium beijerinckii, Clostridium acetobutylicum이다. 대표적인 C6 물질인 n-hexanoic acid는 Clostridium kluyveri와 Megasphaera elsdenii가 다량 생산한다. 미생물 발효에 의해 보고된 n-butanol, n-butyric acid, n-hexanoic acid의 최대 생산량은 각각 21, 55, 19 g/L이었다. 배양과정에서 이들 생산물의 제거는 최종산물억제의 감소로 미생물에 의한 n-butanol, n-butyric acid, n-hexanoic acid의 생산량을 증가시켰다. 특히 C6 물질인 n-hexanoic acid는 n-hexanol로 될 수 있는 고 부가가치 물질로 생물학적 생산 연구가 꾸준히 진행 중인데, 신규한 미생물인 Clostridium sp. BS1은 galactitol을 이용하여 5 g/L의 n-hexanoic acid를 생산하였다.

• Journal of Species Research
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• 제7권2호
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• pp.114-122
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• 2018

As part of a larger study of indigenous prokaryotic species diversity in South Korea, various samples from Namhangang were subjected to analyses. Fresh water, underwater sediment, and moss-inhabiting aerobic and anaerobic bacteria were isolated. 22 of the isolates were identified as unrecorded bacterial species in Korea that had ${\geq}98.7%$ 16S rRNA gene sequence similarity with published species. The aerobic strains isolated were Kurthia gibsonii and Massilia plicata. Also identified were four facultative anaerobic strains: Bacillus hisashii, Enterococcus rotai, Paenibacillus vini, and Pediococcus pentosaceus. 16 strictly anaerobic strains were identified as Bacteroides xylanolyticus, Carnobacterium maltaromaticum, Clostridium argentinense, Clostridium beijerinckii, Clostridium butyricum, Clostridium cavendishii, Clostridium diolis, Clostridium frigidicarnis, Clostridium perfringens, Clostridium saccharoperbutylacetonicum, Clostridium sphenoides, Clostridium subterminale, Cutibacterium acnes, Paraclostridium bifermentans, Prevotella paludivivens, and Romboutsia lituseburensis. Based on the examination of morphological, cultural, physiological, and biochemical properties of the isolates, descriptive information of these previously unrecorded species is provided here.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.205.1-205
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• 2005

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.463-464
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• 2005