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Anti-oxidative, Acetylcholinesterase Inhibitory Activities and Acute Toxicity Study of Nepeta sibirica L.

  • Gonchig Enkhmaa;Gendaram Odontuya;Erdenetsogt Purevdorj;Munkhbat Nomin;Gansukh Enkhjin;Tserendash Chimgee;Chultemsuren Yeruult
    • Natural Product Sciences
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    • v.29 no.2
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    • pp.74-82
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    • 2023
  • Nepeta sibirica L. or Siberian catmint is a medicinal plant species used in Mongolian traditional medicine for curing human different disorders and veterinary practices. The previous study of the whole plant concentrated on the determination of its essential oil composition and reported that the major ones are sesquiterpenes, including nepetalactone. The aim of this study was to reveal a new biological activity of the above-ground parts of N. sibirica L. and compare the activity of different extracts correlating with the content of biologically active compounds and evaluate their toxicity. For this purpose, anti-oxidative and acetylcholinesterase inhibitory activities of the above-ground parts of N. sibirica L. aqueous and ethanol (EtOH) (40%, 70%) extracts were assayed spectrophotometrically. The aqueous extract showed positive anti-oxidative activity by both tested DPPH and FRAP assays with IC50 134.24 ± 1.42 mg/mL and FRAP value 1385.15 ± 8.12 µmol/L at 200 ㎍/mL, in contrast to 40% and 70% EtOH extracts. The 70% EtOH extract presented the highest acetylcholinesterase inhibitory activity (IC50 77.29 ± 0.38 mg/mL) followed by 40% EtOH extract (176.72 ± 0.35 mg/mL) and aqueous extract (275.41 ± 0.23 mg/mL). Total phenolics were found to be gallic acid equivalent, % 3.74 ± 0.05 (70% EtOH), 3.94 ± 0.04 (40% EtOH), and 3.79 ± 0.16 (aqueous), whereas the total flavonoids as a rutin equivalent, % as 2.01 ± 0.12, 1.44 ± 0.17 and 1.99 ± 0.02, each. The aqueous extract showed the best anti-oxidative and lowest activity against the acetylcholinesterase; however, the 70% EtOH extract showed the opposite effects than that of the aqueous. No mortality incidence was visible at various doses, indicating that the oral median lethal dose of aqueous and 70% EtOH extracts were considered greater than 5000 mg/kg. N. sibirica L. belongs to the non-toxic category of the OECD 423 classification.

Design of a designated lane enforcement system based on deep learning (딥러닝 기반 지정차로제 단속 시스템 설계)

  • Bae, Ga-hyeong;Jang, Jong-wook;Jang, Sung-jin
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2022.10a
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    • pp.236-238
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    • 2022
  • According to the current Road Traffic Act, the 2020 amendment bill is currently in effect as a system that designates vehicle types for each lane for the purpose of securing road use efficiency and traffic safety. When comparing the number of traffic accident fatalities per 10,000 vehicles in Germany and Korea, the number of traffic accident deaths in Germany is significantly lower than in Korea. The representative case of the German autobahn, which did not impose a speed limit, suggests that Korea's speeding laws are not the only answer to reducing the accident rate. The designated lane system, which is observed in accordance with the keep right principle of the Autobahn Expressway, plays a major role in reducing traffic accidents. Based on this fact, we propose a traffic enforcement system to crack down on vehicles violating the designated lane system and improve the compliance rate. We develop a designated lane enforcement system that recognizes vehicle types using Yolo5, a deep learning object recognition model, recognizes license plates and lanes using OpenCV, and stores the extracted data in the server to determine whether or not laws are violated.Accordingly, it is expected that there will be an effect of reducing the traffic accident rate through the improvement of driver's awareness and compliance rate.

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A Study on The Classifications of Tie-in Promotion Tools according to Benefit Fit (혜택적합성에 따른 제휴 프로모션 수단의 유형화에 관한 연구)

  • Park, Hyun Hee;Lee, Eun Mi;Jeon, Jung Ok
    • Asia Marketing Journal
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    • v.13 no.4
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    • pp.139-158
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    • 2012
  • This study was intended to classify tie-in promotion tools by the criteria of benefit-fit between consumer and tie-in promotions. Tie-in promotion tools include tie-in price reductions, tie-in coupons, tie-in memberships, tie-in contests, tie-in sweepstakes, tangible and intangible tie-in premiums, tie-in payment terms, tie-in samples, tie-in events(culture event, charity event, experience event) and tie-in fund·rebates. The fit between consumer pursuit benefit and tie-in promotion supplying benefit was used as a classification criteria on the basis of Lee et al.'s study in 2011. For the experiment, one stimuli and 12 scenarioes were developed. 100 pieces of data were obtained for each scenario. As a result, benefit fit was subsequently divided into two factors: hedonic-benefit fit and utilitarian-benefit fit. Tie-in promotion tools were then classified into 4 types: high hedonic benefit-added, high utilitarian benefit-added, low hedonic benefit-added, and low utilitarian benefit-added. In previous research, tie-in promotion type was mainly divided by the evaluative criteria on company's viewpoint such as horizontal/vertical or intra-company/ inter-company, which reflects mutual exclusiveness between two criteria. Whereas, in this study, tie-in promotion type was divided by evaluative criteria on consumer's viewpoint such as hedonic- benefit fit/utilitarian-benefit fit. The classifications in this study practically reflect benefit-added of tie-in promotion type superadded one benefit coexisting two benefits.

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The usefulness of CT for the diagnosis and the fragment fixation of anteroinferior tibiofibular ligament avulsion fracture in ankle fracture (족관절 골절에서 전하 경비 인대 견열 골절의 진단과 골편 고정술을 위한 전산화 단층 촬영의 유용성)

  • Na, Hwa-Yeop;Cho, Kook-Hee;Jung, Yu-Hun
    • Journal of Korean Orthopaedic Sports Medicine
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    • v.10 no.2
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    • pp.78-85
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    • 2011
  • Purpose: To evaluate the usefulness of computerized tomography (CT) for the diagnosis and the fragment fixation of anteroinferior tibiofibular ligament avulsion fracture in ankle fracture. Materials and Methods: We retrospectively studied 108 patients with an ankle fracture who had been checked with plain radiographs and CT from July 2006 to July 2010. They were divided into two groups; patients with (19 patients) and without (89 patients) an avulsion fracture of anteroinferior tibiofibular ligament. The two groups were evaluated with Lauge-Hansen classification, the energy of trauma, and the radiologic indices for syndesmotic injury, and were compared each other. Average follow up periods of two groups were 25 and 23 months each. Those who were unstable at stress test during surgery were divided into fragment fixation of anteroinferior tibiofibular ligament avulsion fracture group (8 patients) and transfixation one (11 patients) according to treatment method. Clinical and radiological results at last follow up were also compared. Results: Fourteen avulsion fractures of anteroinferior tibiofibular ligament were diagnosed by CT only. Incidences of pronation-external rotation injury, high energy trauma, positive radiologic indices for syndesmotic injury were significantly higher in patients with an avulsion fracture of anteroinferior tibiofibular ligament than those without it. Clinical and radiological results were satisfactory in both groups at last follow up, and were not significantly different between them. Conclusion: In patients who have an ankle fracture by pronation-external rotation injury, high energy trauma, or with positive radiologic indices for syndesmotic injury, CT is useful for diagnosis of an avulsion fracture of anteroinferior tibiofibular ligament. Fragment fixation of anteroinferior tibiofibular ligament avulsion fracture is a useful treatment option for syndesmotic injury.

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Automated Analyses of Ground-Penetrating Radar Images to Determine Spatial Distribution of Buried Cultural Heritage (매장 문화재 공간 분포 결정을 위한 지하투과레이더 영상 분석 자동화 기법 탐색)

  • Kwon, Moonhee;Kim, Seung-Sep
    • Economic and Environmental Geology
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    • v.55 no.5
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    • pp.551-561
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    • 2022
  • Geophysical exploration methods are very useful for generating high-resolution images of underground structures, and such methods can be applied to investigation of buried cultural properties and for determining their exact locations. In this study, image feature extraction and image segmentation methods were applied to automatically distinguish the structures of buried relics from the high-resolution ground-penetrating radar (GPR) images obtained at the center of Silla Kingdom, Gyeongju, South Korea. The major purpose for image feature extraction analyses is identifying the circular features from building remains and the linear features from ancient roads and fences. Feature extraction is implemented by applying the Canny edge detection and Hough transform algorithms. We applied the Hough transforms to the edge image resulted from the Canny algorithm in order to determine the locations the target features. However, the Hough transform requires different parameter settings for each survey sector. As for image segmentation, we applied the connected element labeling algorithm and object-based image analysis using Orfeo Toolbox (OTB) in QGIS. The connected components labeled image shows the signals associated with the target buried relics are effectively connected and labeled. However, we often find multiple labels are assigned to a single structure on the given GPR data. Object-based image analysis was conducted by using a Large-Scale Mean-Shift (LSMS) image segmentation. In this analysis, a vector layer containing pixel values for each segmented polygon was estimated first and then used to build a train-validation dataset by assigning the polygons to one class associated with the buried relics and another class for the background field. With the Random Forest Classifier, we find that the polygons on the LSMS image segmentation layer can be successfully classified into the polygons of the buried relics and those of the background. Thus, we propose that these automatic classification methods applied to the GPR images of buried cultural heritage in this study can be useful to obtain consistent analyses results for planning excavation processes.

A study on Classification of Temporarily Access Group about Sanitation Workers in Nuclear Medicine Department (핵의학과 환경미화원의 일시 출입자 분류에 대한 고찰)

  • Yoo, Jae-Sook;Jang, Jeong-Chan;Kim, Ho-Seong
    • The Korean Journal of Nuclear Medicine Technology
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    • v.16 no.1
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    • pp.50-56
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    • 2012
  • Purpose: Those who access to the nuclear medicine department are classified as radiation workers, temporarily access group, and occasional access group as defined by the atomic energy law. The radiation workers and temporarily access people wear a personal radiation dosimeter for checking their own radiation absorbed dose periodically. However, because of the sanitation workers, classified as temporarily access group, who are working in the nuclear medicine department are moved in a cycle with other departments and their works are changeful, it is hard to control their radiation absorbed dose. Thus, this study is going to examine the state of the sanitation worker's radiation absorbed dose, and then make sure whether they are classified as temporarily access group or not. Materials and methods: In the first instance, the first sanitation worker who works in vitro laboratory and PET room and the second sanitation worker who works in gamma camera rooms (invivo room) wore radiation dosimeter-OSL(Optically Stimulated Luminescence)- to measure their own radiation absorbed dose during work time from May to June 2011. Secondly, this study was taken place 5 places in gamma camera rooms, 2 places in PET bed room, operating room, waiting room and cyclotron room in PET and 4 places in vitro laboratory. And then to measure the radiation space dose rate, it is measured 10 times each of places as sanitation worker's work flow by using radiation survey meter. Results: The radiation absorbed dose on OSL of the first c who works in vitro laboratory and PET room and the second one who works in gamma camera rooms are 0.04, 0.02 mSv per month respectively. That means the estimated annual radiation absorbed doses are less than 1mSv as 0.48, 0.24 mSv/yr respectively. The radiation space dose rates as sanitation worker's work flow using survey meter are 0.0037, 0.0019 mSv/day, so the estimated annual radiation absorbed dose are 0.93, 0.47 mSv/yr respectively. The weighted exposure dose of first sanitation worker of each places are 1.62% in cyclotron room, 3.88% in waiting room, 2.39% in operating room, 81.01% in bed room of PET and 11.01% in vitro laboratory. The weighted exposure dose of second sanitation worker of each places are 45.22% in radiopharmaceutical laboratory, gamma 30.64% in camera rooms, 15.65% in waiting room, 8.49% in reading room. Conclusion: The annual radiation absorbed doses on OSL of both sanitation workers are less than 1 mSv per year and the annual radiation absorbed doses by using survey meter are less than 1mSv either, but close up to 1 mSv. Thus, to clarify whether the sanitation workers are temporarily access group or not, and to be lessen their s radiation absorbed dose, they should be educated about management of radiation and modified their work flow or work time appropriately, their radiation absorbed dose would be lessen certainly.

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Properties of Indigenous Korean Paper(Hanji) - Classification of Oebal(single frame)Papermaking Methods - (토착한지의 특성 - 외발 초지법 분류를 중심으로 -)

  • Cheon, Cheol;Kim, Seong-Ju;Jin, Young-Mun
    • Journal of the Korean Wood Science and Technology
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    • v.27 no.1
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    • pp.88-104
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    • 1999
  • This study was carried out to classify the Hanjis into three groups that were indigenous Hanji, traditional Hanji, and improved Hanji handmade by paper making method according to the physical properties of each paper sheet such as tensile, bursting and tearing strength, folding endurance and fiber orientation in each layer. The results obtained were summarized as follows: 1. The multi-layered Hanjis made by "Oebal" Hanji making method in different direction of fiber orientation have good properties in tearing resistance. 2. The multi-layered Hanji in different direction of fiber orientation has good properties in the tearing resistance, but the burst index and the breaking length results were lower than the single layered Hanjis. 3. The different fiber orientation and multi-layered method didn't increase, the three indexes(burst index, tear index, breaking length). Only, the different direction of fiber orientation decreased the difference of width and length strength (tensile, tear) of the Hanji. 4. "Dochim"(Korean finishing touch process for indigenous Hanji by fulling round sticks) greatly increase folding endurance(double folds, not $log_{10}$) and good effect to tensile strength and burst strength. 5. The today's Oebal Hanji were the maximum of 2 layers and the indigenous Oebal Hanji were 16 layers the maximum. In addition, average of the indigenous Oebal Hanji was 4 layers(all 4-layer Hanji were the different fiber orientation of each layer). 6, The indigenous Hanji(multi-layered, and different fiber orientation) was good condition with "Dochim". Dochim increased tensile strength and burst strength of the indigenous Hanji. So the three-strength indexes were similar level("--"). 7. When the number of layer which were same fiber orientation increase, the increased Hanji became similar strength pattern("V", breaking length and burst index was higher than tear index) with "Ssangbal" Hanji. 8. The single layered papers that made by "Oebal" Hanji making method were similar strength pattern with Ssangbal Hanji. 9. There was no way to find the width and length direction of multi-layered Hanji by comparison between the difference of tensile strength and the difference of tearing resistance. 10. The compared pattern of tensile strength and tearing resistance of indigenous Oebal Hanji was different from today's Oebal Hanji. Especially, the tearing resistance of all indigenous Oebal Hanji(16 samples) was stronger on width of tearing resistance. And in the half of indigenous Oebal Hanji samples, the width of tensile strength and tearing resistance was stronger than length strength (Indigenous Oebal: '$\ulcorner\lrcorner$' 50%, '$\bigcup$' 50% $\leftrightarrow$ Today's Oebal: '$\ulcorner\lrcorner$' 12%, '$\bigcup$'6%, '$\llcorner\urcorner$'17%, '$\bigcap$'65%). In 65% today's Oebal, the length direction of tensile strength and tearing resistance was stronger than the width direction.

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Actual Vegetation and Plant Community Structure of Geumsun Valley and Weonjeok Valley in Naejangsan(Mt.) National Park, Korea (내장산국립공원 금선계곡과 원적계곡의 현존식생 및 식물군집구조)

  • Bae, Ki-Wook;Lee, Kyong-Jae;Han, Bong-Ho;Kim, Jong-Yup;Jang, Jae-Hoon
    • Korean Journal of Environment and Ecology
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    • v.26 no.3
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    • pp.412-425
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    • 2012
  • This study investigated the actual vegetation and plant community structure of Geumsun valley and Weonjeok valley of Naejangsan(Mt.) National Park to provide the data for park management. As a result of analysis of actual vegetation, Quercus variabilis community(47.3%) and Quercus serrata community(17.0%) are widely distributed but Carpinus tschonoskii community(7.5%) and Zelkova serrata community(6.9%) are distributed in valley forests. Plant communities were divided into 6 communities of Fraxinus mandshurica, Carpinus tschonoskii, Carpinus tschonoskii-Platycarya strobilacea-Zelkova serrata, Zelkova serrata, deciduous broad-leaved mixture(Zelkova serrata-Lindera erythrocarpa-Acer palmatum), and Lindera erythrocarpa-Cornus walteri according to the analysis of TWINSPAN of classification using 20 plots($400m^2$). Geumseon valley is composed of boulder stone area of average slope $20^{\circ}$, and there were distributed Fraxinus mandshurica community of 86 years old, Carpinus tschonoskii community in age from 56 to 79, and Carpinus tschonoskii-Platycarya strobilacea-Zelkova serrata community in age from 48 to 71. Weonjeok valley is composed of boulder stone area of average slope $11^{\circ}$, and there were distributed Zelkova serrata community in age from 52 to 71, deciduous broad-leaved mixture community in age from 49 to 70, and Lindera erythrocarpa-Cornus walteri community in age from 43 to 51. Ecological succession of each community was predicted to maintain same state. The Shannon's species diversity showed from 0.8220(Fraxinus mandshurica community) to 1.3850(Carpinus tschonoskii community) per unit area of $400m^2$.

A Comparative Study on Chewing Movement in Normal Occlusion and Skeletal Class III Malocclusion (정상교합자와 골격성 III급 부정교합자의 저작운동형태의 비교)

  • SUNG, Kee-Hyuk;SUNG, Jae-Hyun
    • The korean journal of orthodontics
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    • v.27 no.5 s.64
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    • pp.801-813
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    • 1997
  • A comparative study was made on the chewing movements of normal occlusion and skeletal class m malocclusion. Thirty normal occlusion subjects and twenty skeletal class III malocclusion patients were given chewing gums for the study : using BioPAK system, the chewing movement on the frontal plane was recorded and analyzed. With a typical chewing path chosen representing each subject, chewing width, opening distance, opening and closing angles, maximum opening and closing velocities were observed. Seven characteristic patterns were classified based on the types of chewing paths. The followings are the results : 1. Compared with the normal occlusion group, the skeletal class III malocclusion group showed more varied and vertical chewing patterns. 2. In comparision of chewing widths, skeletal class m malocclusion group showed narrower path than the normal occlusion group(p<0.01). 3. In opening distance, skeletal class III malocclusion group appeared shorter than the normal occlusion group without statistical significance(p>0.05). 4. In opening and closing angles, skeletal class III malocclusion group showed more acute angles than the normal occlusion group(p<0.01). 5. In maximum opening and closing velocities, skeletal class III malocclusion group was slower than the normal occlusion group but with no statistical significance(P>0.05). 6. In the classification of chewing movement pattern, the normal occlusion group had Type II as the highest rate at 73.4% ; in skeletal class III malocclusion group, the highest rate was Type III at 35.0%, followed by Type II at 30.0% 7. In the classification of chewing movement pattern, Type IV(chopping type)of skeletal class III malocclusion group showed a higher rate with 25.0% over 3.3% of normal occlusion group.

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A study of quality of working life to dental hygienist's (치과위생사의 근로생활의 질(QWL)에 관한 연구)

  • Oh, Hye-Seung;Kim, Eun-Hee
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.2
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    • pp.375-392
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    • 2010
  • Objectives : Dental hygienist's work satisfaction and stress affect the overall quality of work life(QWL). Therefore, this research is intended to suggest fundamental data to improve QWL by finding out characteristics of each work satisfaction and stress element. To this end, a total of 327 dental hygienists working at general hospitals, university hospitals, dental hospitals and dental clinics across Seoul, Gyeonggi and Incheon were surveyed. Results of survey are as follows. Methods : The collected data were analyzed by using an SPSS 12.0 statistical program, obtaining the following results. The collected data conducted a questionnaire survey for 327 dental hygienists who work at the hospitals, university hospitals, dental hospitals, and dental clinics located at Seoul, Gyeonggi-do, and Incheon district from January until March, 2009, and drew the conclusions as follows. Result : 1. Demographic characteristics, income from 1.5 to 1.99 million were the whole lot, more than 2 million to less than 1.5 million was similar. Marital status Married Unmarried higher than the atheist religion, Christianity, Catholicism, Buddhism, and other, respectively. Classification by level of education in the college graduate, university graduate, graduate diploma, respectively. 2. Are working in a job-related characteristics of dentistry, dental hospital, general and university hospital, respectively. The making in position, Mount, contractor, responsible, senior, was an intern in the order. The five-day workweek whether working at night and is not going to care whether the conduct was similar. Classification of working hours and 8 hours, 8 hours, 8 hours or less orderly, and total of less than 1-3 years of clinical experience, 5 years, less than one year, less than 3-5 years, respectively. 3. There comes out a significant difference according to age, income, position, gross clinical experience, and whether to put night shift into practice in job stability in terms of the quality subsequent to general characteristics(p<.05). 4. There comes out a significant difference according to marital status, one's place of work, position, whether to put a five-day workweek into practice in work environment and benefits package in terms of the quality subsequent to general characteristics (p<.05). 5. There comes out a significant difference according to age, marital status, income, position, and gross clinical experience in education & training and benefits packages in terms of the quality subsequent to general characteristics(p<.05). 6. There comes out a significant difference according to whether to put night medical treatment into practice in social usefulness in terms of the quality subsequent to general characteristics(p<.05). 7. There comes out a significant difference according to marital status, income, one's place of work, gross clinical experience, work hours, and whether to put a five-day workweek into practice in leisure activity in terms of the quality subsequent to general characteristics(p<.05). 8. There comes out a significant difference according to income, one's place of work, and position in wage level in terms of the quality subsequent to general characteristics(p<.05). 9. There was no significant difference in all items related to human relations and free communication in terms of the quality subsequent to general characteristics(p>.05). Conclusions : It is necessary to analyze factors related to work satisfaction and stress in order to improve dental hygienist's quality of work life. Hospitals must support them systematically and institutionally and related organizations must conduct practical research.