• Title/Summary/Keyword: Chungpaesagan-tang

Search Result 5, Processing Time 0.021 seconds

Effect of Chungpaesagan-tang on cerebral Ischemic Damage Induced by MCAO in Rats (청폐사간탕(淸肺潟肝湯)이 MCAO에 의한 흰쥐의 뇌허혈 손상에 미치는 영향)

  • Jung, Gwang-Sik;Kim, Bum-Hoi;Hwang, Won-Duk
    • Journal of Society of Preventive Korean Medicine
    • /
    • v.13 no.1
    • /
    • pp.13-27
    • /
    • 2009
  • This study aimed to validate neuroprotective effect of Chungpaesagan-tang on the early stage of cerebral ischemic damage. Cerebral ischemic damage was induced by the middle cerebral artery occlusion (MCAO) for 2 hours in the Sprague-Dawley rats. Water extract of Chungpaesagan-tang(8.7g/kg) was administered orally twice at 1 and 4 hours after the MCAO. Neurological score was tested at 3 and 24 hours after the MCAO and Chungpaesagan-tang administration. At 24 hours after the MCAO, infarct volume and edema ratio was evaluated with the TTC staining. Apoptotic cell death in cerebral cortex and caudate putamen was observed with cresyl violet staining and TUNEL labeling. Bax expression in the MCAO rat brain was stained with immunohistochemistry. Chungpaesagan-tang improved neurological and behavioral impairment of the MCAO rats and reduced infarct area, infarct volume and brain edema formation. Chungpaesagan-tang attenuated cell death percentage in cortex penumbra and reduced TUNEL positive cells in cortex penumbra and in caudate putamen of the MCAO rats. Chungpaesagan-tang reduced Bax positive neurons in caudate putamen and reduced c-Fos positive neurons in cortex penumbra of the MCAO rats. Chungpaesagan-tang intensified neuronal HSP72 expression in cortex penumbra of the MCAO rats. In results, Chunpaesagan-tang reduces infarct volume and edema formation through anti-apoptotic effect. This result suggests that Chunapaesagan-tang has an adequate neuroprotective effect on the early stage of cerebral ischemic damage.

  • PDF

Effect of Chungpaesagan-tang on Ischemic Damage in Organotypic Hippocampal Slice Culture (청폐사간탕(淸肺瀉肝湯)이 뇌해마 조직배양의 신경세포 자연사에 미치는 영향)

  • Lee, Min-Young;Ku, Ja-Seung;Kim, Sung-Hoon;Kim, Yoon-Bum;Kim, Sun-Yeou;Choi, Hyeon;Sohn, Young-Joo;Jung, Hyuk-Sang;Sohn, Nak-Won
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.22 no.4
    • /
    • pp.771-777
    • /
    • 2008
  • Chungpaesagan-tang which is used for treating patients of brain in cerebrovascular disease frequently from clinical doctor has not reported about the effect of neuronal aptosis caused of brain ischemia. The aim of this study is to investigate effect of Chungpaesagan-tang protecting neuronal cells from being damaged by brain ischemia through using organotypic hippocampal slice cultures. We caused ischemic damage to organotypic hippocampal slice cultures by oxygen and glucose deprivation. And added Chungpaesagan-tang extract to cultures. thereafter we measured area percentage of propidium iodide (PI)-stained neuronal cell, lactate dehydrogenase (LDH) levels in culture media and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Area percentage of PI-stained neuronal cells and count of TUNEL-positive cells in CA1 and DG area of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. LDH levels in culture media of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. Within pertinent density level, Chungpaesagan-tang has cell protection effect that prevents brain ischemia damaging neuronal cells and apoptosis increasing.

Experimental Studies on the Effects of Taeŭmin Chungpaesagan-Tang (태음인(太陰人) 청사사간탕(淸師瀉肝湯)의 효능(效能)에 대(對)한 실험적(實驗的) 연구(硏究))

  • Yoon, Byung Soo
    • Journal of Sasang Constitutional Medicine
    • /
    • v.2 no.1
    • /
    • pp.135-147
    • /
    • 1990
  • In order to investigate experimentally the clinical effects. of $Tae{\breve{u}}min$ Chungpaesagan-Tang (太陰人 淸肺瀉肝湯) that was prescribed to cure the $Kansuy{\breve{o}}lriy{\breve{o}}lby{\breve{o}}ng$ (肝受熱裏熱病) of $Tae{\breve{u}}min$, the author experimented various activities of mixed extract from $Tae{\breve{u}}min$ Chungpaesagan-Tang by the methods prescribed in the experimental part. The results of the studies were summarized as follows; 1. In the acetic acid method experiment and the pressing hind paw method experiment, analgesic effects were noted. 2. The prolongation of the duration of hypnosis induced by Pen to barbital-sodium was noted. 3. Spontaneous movement of the isolated mice ileum was markedly suppressed. and Contraction to induced by acetylcholine, Barium chloride was Inhibited. 4. Antipyretic action by Thyphoid vaccine were noted in mice. 5. Significant anti-edemic effects were recognized on the edema induced by yeast. 6. Hypotensive action due to vaso-dilatating of normal rats and rabbits were noted.

  • PDF

Effect of Chungpaesagan-tang on Ischemic Damage Induced by Middle Cerebral Artery Occlusion in Diabetic Rats (청폐사간탕이 탕요유발 흰주의 뇌허혈손상에 미치는 영향)

  • Jeong Chun-geun;Kim Eun-Young;Shin Jung-Won;Sohn Youngjoo;Lee Hyun-Sam;Jung Hyuk-Sang;Sohn Nak-Won
    • The Journal of Korean Medicine
    • /
    • v.26 no.2 s.62
    • /
    • pp.217-230
    • /
    • 2005
  • Objectives: Chungpaesagan-tang (CPSGT), which is frequently used for treating patients of cerebrovascular disease, has not been reported by clinical doctors concerning the effect of neuronal aptosis caused by brain ischemia. To study the effect of CPSGT on focal cerebral ischemia in normal and diabetic rats and SHR, focal cerebral ischemia was induced by transient MCAO, and after onset CPSGT was administrated. Methods: Rats (Sprague-Dawley) were divided into four groups: sham-operated group, MCA-occluded group, CPSGT­administrated group after MCA occlusion, and normal group. The MCA was occluded by intraluminal method. CPSGT was administrated orally twice (l and 4 hours) after middle cerebral artery occlusion. All groups were sacrificed at 24 hours after the surgery. The brain tissue Was stained with $2\%$ triphenyl tetrazolium chloride (TTC) or $1\%$ cresyl violet solution, to examine effect of CPSGT on ischemic brain tissue. The blood samples were obtained from the heart.~. Tumor necrosis $factor-\alpha$ level and interleukin-6 level of serum was measured from sera using enzyme-linked immunoabsorbent assay (ELISA). Then changes of immunohistochemical expression of $TNF-\alpha$ in ischemic damaged areas were observed. Results: In NC+MCAO+CP and DM+MCAO+CP, CPSGT significantly (p<0.01) decreased the number of neuron cells compared to the control group. CPSGT markedly reduced (p<0.01) the infarct size of the forebrain in distance from the interaural line on cerebral ischemia in diabetic rats. CPSGT significantly reduced the $TNF-\alpha$ expression in penumbra region of damaged hemisphere in diabetic rats. Conclusions: CPSGT had a protective effect on cerebral ischemia in SD rats, especially in diabetic rats compared with normal SD rats.

  • PDF

Effect of Chungpaesagan-tang on ischemic damage induced by MCAO in spontaneously hypertensive rats

  • Kim, Ko-Eun;Kim, Soo-Yong;Kim, Eun-Young;Kim, Bum-Hoi;Shin, Jung-Won;Lee, Hyun-Sam;Sohn, Young-Joo;Jung, Hyuk-Sang;Sohn, Nak-Won
    • Advances in Traditional Medicine
    • /
    • v.8 no.4
    • /
    • pp.430-439
    • /
    • 2008
  • Chungpaesagan-tang (CPSGT) is most frequently used to treat ischemic brain injury in tradition Korean medicine. Clinically, cerebral ischemia is likely to be accompanied by preexisting or complicating disease. However, animal models used to examine the effects of herbal medicines on cerebral ischemia have not given this issue sufficient consideration. The present study was undertaken to determine the effects of CPSGT on focal cerebral ischemia in normal and SHR rats subjected to transient middle cerebral artery occlusion (MCAO). Animals were divided into four groups: Normal (Sprague-Dawley) rats subjected to MACO (the NC+MCAO group), normal rats subjected to MCAO and then administered CPSGT (NC + MCAO + CP), SHR rats subjected to MCAO (SHR + MCAO), and SHR rats subjected to MCAO and then administered CPSGT (SHR + MCAO + CP). MCAO was performed using the intraluminal method. CPSGT was administrated orally twice (1 and 4 h) after MCAO. All animals were sacrificed at 24 h postoperatively. Brain tissues were stained with hematoxylin & eosin, to examine the effect of CPSGT on ischemic brain tissues. In addition, changes in TNF-$\alpha$ expression in ischemic areas were examined by immunostaining. CPSGT was found to significantly reduce infarction areas in normal and SHR rats and infarction volumes in SHR rats. Similarly, CPGST markedly increased neuron numbers and sizes in all treated groups, except cell sizes in SHRs. Furthermore, CPSGT reduced TNF-$\alpha$ expression in MCAO administered SHR rats. The findings of the present study suggest that CPSGT effectively ameliorates neuron damage caused by MACO-induced cerebral ischemia, and that it has a significant neuroprotective effect after cerebral ischemia in SHR.