• Applied Microscopy
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• 제43권4호
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• pp.133-139
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• 2013

This study was carried out to investigate the effects of pycnogenol (PYC) on the cutaneous wound healing of the mice. The wounds were extracted on days 1, 3, 5, and 7 post-injury for histomorphometrical analysis including wound area, infiltrating inflammatory cells, wound contracture including collagen deposition. As the result, the wound area of PYC-treated group was larger than the control group on days 1 to 7. Inflammatory cells in the PYC-treated wounds were decreased at day 1 compared to the control wound tissue. From day 3 to 7, there was no significant difference between the control and the PYC-treated skin wounds. Though the degree of contraction in the PYC-treated group was lower than that of the control group from days 1 to 5, but appeared significantly higher on day 7. Compared to the control group, collagen accumulation in the PYC-treated group was higher than that of the control group from days 5 to 7. From this result, it may support the possibility that PYC would be useful agent for early inflammatory response and matrix remodeling phase of the skin wounds.

• 한국지능시스템학회논문지
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• 제18권1호
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• pp.66-71
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• 2008

최근 멀티미디어 서비스는 동영상 압축 기술 및 네트워크의 발달을 기반으로 하여 다양한 응용 서비스를 제공하고 있으며, 이 중 화상 회의 시스템은 이 두 가지 기술이 효과적으로 사용된 대표적인 예이다. 원격 사용자간의 원활한 의사전달을 위해 고려된 화상회의 시스템은 효과적인 응용 서비스로 분류되고 있지만, 이러한 서비스 제공을 위한 기술을 이용하여 빈도가 훨씬 많은 일반적인 회의를 지원하는 응용서비스는 드문 편이다. 본 논문에서는 얼굴 정보와 화자 정보를 기반으로 오프라인 회의를 보조하는 시스템을 제안한다. 제안된 시스템은 3개의 마이크와 웹캠을 이용하여 화자의 위치를 파악하고 웹캠에서 얻어진 정보를 이용하여 얼굴 영역 정보를 분석하고 인식한 후 화자 정보를 추출하여 발언자들을 추적하여 기록하는 기능을 제공한다.

• 해양환경안전학회:학술대회논문집
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• 해양환경안전학회 2017년도 추계학술발표회
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• pp.164-164
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• 2017

국내 해양관련 HNS 데이터베이스의 정보를 구축하는 중에 확인된 문제점들은 크게 두 가지로 요약된다. 첫 번째는 물질정보가 없는 경우로서, 많은 경우 자연물질로 다양한 성분을 함유하고 있으며, 이외에도 이성질체 및 동족체 화합물로서 확인된다. 두 번째로는 국내 HNS DB의 출처가 다양하여, 정제되지 않은 경우인데 물질정보의 환경조건이 상이하거나 같은 조건에서도 서로 다른 정보가 혼재하는 경우이다. 이러한 문제점을 해결하기 위해, 유럽의 GESAMP에서 제시한 보고서와 미국 CAMEO를 참조하여 누락정보를 확보하였으며 두 데이터베이스의 명명법의 차이에서 오는 누락 정보를 두 데이터베이스의 유사명 자료비교와 화학구조를 이용해 확인하였다. 자연물질과 그룹화합물들도 주요성분의 자료를 활용하였으며, 이성질체의 경우 단일성분의 자료를 기입하거나, 위험성이 높은 성분의 자료를 선택해서 기입하였다. 물질정보 중 IMDG 등급의 경우 주위험성외에 부위험성의 중요성을 고려하여 HNS DB에 새로 기입하였으며, 부록으로 HS 번호 부여 및 해양환경거동분류를 첨부하였다. 향후 아직 남아있는 누락정보의 확보를 위해서는 GESAMP circular report를 확보하도록 할 필요성이 있다고 판단된다.

• Journal of Microbiology and Biotechnology
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• 제31권5호
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• pp.705-709
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• 2021

Porphyromonas gingivalis (P. gingivalis) is a major bacterial pathogen that causes periodontitis, a chronic inflammatory disease of tissues around the teeth. Periodontitis is known to be related to other diseases, such as oral cancer, Alzheimer's disease, and rheumatism. Thus, a precise and sensitive test to detect P. gingivalis is necessary for the early diagnosis of periodontitis. The objective of this study was to optimize a rapid visual detection system for P. gingivalis. First, we performed a visual membrane immunoassay using 3,3',5,5'-tetramethylbenzidine (TMB; blue) and coating and detection antibodies that could bind to the host laboratory strain, ATCC 33277. Antibodies against the P. gingivalis surface adhesion molecules RgpB (arginine proteinase) and Kgp (lysine proteinase) were determined to be the most specific coating and detection antibodies, respectively. Using these two selected antibodies, the streptavidin-horseradish peroxidase (HRP) reaction was performed using a nitrocellulose membrane and visualized with a detection range of 10³-10⁵ bacterial cells/ml following incubation for 15 min. These selected conditions were applied to test other oral bacteria, and the results showed that P. gingivalis could be detected without cross-reactivity to other bacteria, including Streptococcus mutans and Escherichia fergusonii. Furthermore, three clinical strains of P. gingivalis, KCOM 2880, KCOM 2803, and KCOM 3190, were also recognized using this optimized enzyme immunoassay (EIA) system. To conclude, we established optimized conditions for P. gingivalis detection with specificity, accuracy, and sensitivity. These results could be utilized to manufacture economical and rapid detection kits for P. gingivalis.

• 동의신경정신과학회지
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• 제34권4호
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• pp.433-444
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• 2023

Objectives: This case study aimed to provide evidence of the combined use of Korean Medicine treatments, including electroacupuncture (EA) on scalp acupoints, as a useful intervention for post-stroke delirium. Methods: The patient in this study was diagnosed with Cerebral Infarction in Right Corpus Callosum and post-stroke delirium. The patient received eight sessions of EA on scalp acupoints, rehabilitation medicine treatment, and other Korean Medicine treatments such as acupuncture and herbal medicine. Evaluation of treatment effectiveness was done mainly through the Memorial Delirium Assessment Scale Korean (MDAS-K) and Mini-Mental State Examination Korean (MMSE-K). Results: MDAS-K score dropped from 20 to 9 and MMSE-K score increased from 15 to 21 during hospitalization, showing improvement in symptoms. Conclusions: Combined use of Korean medicine treatments including EA on scalp acupoints may be an effective treatment for post-stroke delirium.

• 한국음향학회지
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• 제25권6호
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• pp.305-311
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• 2006

본 논문에서는 성층화된 천해 음향도파관 환경에서 빔-시간 강도 패턴을 이용한 음원 거리추정 기법인 배열 불변성 이론에 대한 성능을 분석하였다. 기존의 수중음원 위치추적 방법으로 잘 알려진 정합장 처리, 음향도파관 불변성 추정법 등에 비해 계산량을 절감 할 수 있고, 배열 이득을 충분히 활용할 수 있는 이점을 가진다. 또한, 기타 간섭 신호원의 영향을 크게 받지 않는 상황에서는 수중 환경에 대한 자세한 사전정보가 요구되지 않는다. 기존의 수중 음원 위치 추정법들에 비해 간단하고 순간적인 음원의 거리 추정이 가능한 배열 불변성 방법의 성능을 분석하기 위해 모의 음파 전달 음장을 이용한 시뮬레이션을 수행하였다. 광대역을 가지는 수중 충격성 음원에 대하여 SNR 에 따른 거리 추정 결과를 나타내었고 고주파 신호의 다중경로 전파 현상을 억제시키기 위해 공간 스무딩 기법을 적용하였다. 거리추정 성능을 분석한 결과, SNR 10dB의 환경에서 20% 내의 오차율을 보였다.

• 한국음향학회지
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• 제26권8호
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• pp.415-425
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• 2007

본 논문에서는 간섭 패턴 정합을 이용한 수중 광대역 음원의 위치 추정법을 제안한다. 두 개의 센서 스펙트로그램에 나타나는 간섭 패턴의 정합을 통해 음원과 두 센서간의 상대적인 거리비를 추정하고 이를 아폴로니오스 원의 방정식에 적용하였다. 아폴로니오스의 원은 두 정점에 이르는 거리비가 일정한 값으로 운동하는 점의 자취로 정의되며, 음원의 위치로 추정 가능한 궤적을 나타낼 수 있다. 그러나 아폴로니오스의 원 하나만으로는 정확한 음원의 위치추정이 어렵다. 그러므로 또 하나의 위치 궤적을 추정 할 수 있는 방정식이 필요하다. 따라서 음원과 두 센서간의 신호도달 시간차이를 이용한 쌍곡선의 방정식을 도입하여 최종적인 음원의 위치는 두 방정식의 교점의 좌표로 추정하였다. 제안된 방법의 성능 평가를 위하여 모의실험을 통해 위치 추정 오차율을 분석하고, 해상실험을 통하여 실제 적용 가능성을 분석하였다. 모의실험 및 해상실험 결과 본 논문에서 제안된 위치 추정 알고리즘이 오차율 10% 이내의 성능을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제17권8호
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• pp.1271-1283
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• 2007

A fibrinolytic protease (PoFE) was purified from the cultured mycelia of the edible oyster mushroom Pleurotus ostreatus, using a combination of various chromatographies. The purification protocol resulted in an 876-fold purification of the enzyme, with a final yield of 6.5%. The apparent molecular mass of the purified enzyme was estimated to be 32 kDa by SDS-PAGE, fibrin-zymography, and size exclusion using FPLC. The optimal reaction pH value and temperature were pH 6.5 and $35^{\circ}C$, respectively. PoFE effectively hydrolyzed fibrinogen, preferentially digesting the $A{\alpha}$-chain and the $B{\beta}$-chain over the ${\gamma}$-chain. Enzyme activity was enhanced by the addition of $Ca^{2+},\;Zn^{2+},\;and\;Mg^{2+}$ ions. Furthermore, PoFE activity was potently inhibited by EDTA, and it was found to exhibit a higher specificity for the chromogenic substrate S-2586 for chymotrypsin, indicating that the enzyme is a chymotrypsin-like metalloprotease. The first 19 amino acid residues of the N-terminal sequence were ALRKGGAAALNIYSVGFTS, which is extremely similar to the metalloprotease purified from the fruiting body of P. ostreatus. In addition, we cloned the PoFE protein, encoding gene, and its nucleotide sequence was determined. The cDNA of cloned PoFE is 867 nucleotides long and consists of an open reading frame encoding 288 amino acid residues. Its cDNA showed a high degree of homology with PoMEP from P. ostreatus fruiting body. The mycelia of P. ostreatus may thus represent a potential source of new therapeutic agents to treat thrombosis.

• Journal of Microbiology
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• 제44권6호
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• pp.622-631
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• 2006

In this study we purified a fibrinolytic enzyme from Cordyceps militaris using a combination of ion-exchange chromatography on a DEAE Sephadex A-50 column, gel filtration chromatography on a Sephadex G-75 column, and FPLC on a HiLoad 16/60 Superdex 75 column. This purification protocol resulted in a 191.8-fold purification of the enzyme and a final yield of 12.9 %. The molecular mass of the purified enzyme was estimated to be 52 kDa by SDS-PAGE, fibrin-zymography, and gel filtration chromatography. The first 19 amino acid residues of the N-terminal sequence were ALTTQSNV THGLATISLRQ, which is similar to the subtilisin-like serine protease PR1J from Metarhizium anisopliae var. anisopliase. This enzyme is a neutral protease with an optimal reaction pH and temperature of 7.4 and $37^{\circ}C$, respectively. Results for the fibrinolysis pattern showed that the enzyme rapidly hydrolyzed the fibrin $\alpha$-chain followed by the $\gamma$-$\gamma$ chains. It also hydrolyzed the $\beta$-chain, but more slowly. The A$\alpha$, B$\beta$, and $\gamma$ chains of fibrinogen were also cleaved very rapidly. We found that enzyme activity was inhibited by $Cu^{2+}$ and $Co^{2+}$, but enhanced by the additions of $Ca^{2+}$ and $Mg^{2+}$ ions. Furthermore, fibrinolytic enzyme activity was potently inhibited by PMSF and APMSF. This enzyme exhibited a high specificity for the chymotrypsin substrate S-2586 indicating it's a chymotrypsin-like serine protease. The data we present suggest that the fibrinolytic enzyme derived from the edible and medicinal mushroom Cordyceps militaris has fibrin binding activity, which allows for the local activation of the fibrin degradation pathway.

• 한국임상수의학회지
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• 제28권4호
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• pp.403-407
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• 2011

A form of polycystic ovary (PCO) resembling some aspects of the human PCO syndrome (PCOS) can be induced in rats by a single injection of estradiol valerate (EV). An increase in sympathetic outflow to the ovary precedes, by several weeks, the appearance of cysts, suggesting the involvement of a neurogenic component in the pathology of this ovarian dysfunction. To test the hypotheses that the change in sympathetic tone is related to an augmented production of hippocampal and/or ovarian nerve growth factor (NGF), and that this abnormally elevated production of NGF contributes to the induction of PCOS induced by EV. The animals were sacrificed after PCOS induction and the ovaries and hippocampus were sectioned and compared to the normal control. The expression of NGF was measured by immunohistochemical staining and Western blot analysis in the ovaries and hippocampus. EV-induced PCOS showed significant increase of ovarian NGF expression. Immunohistochemical expression of NGF was confined to the follicular cells and interstitial cells. Hippocampal NGF expression was not significantly changed. In conclusion EV-induced PCOS was related to the ovarian sympathetic activation which was mediated by NGF.