• 한국식품영양학회지
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• 제31권3호
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• pp.378-387
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• 2018

This study investigated the volatile flavor composition of essential oils from Chrysanthemum zawadskii var. latilobum Kitamura and Aster yomena Makino. The essential oils obtained by the hydrodistillation extraction method from the aerial parts of the plants were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). One hundred (95.04%) volatile flavor compounds were identified in the essential oil from the C. zawadskii var. latilobum Kitamura. The major compounds were valencene (10.82%), ${\delta}$-cadinol (9.77%), hexadecanoic acid (8.70%), 2-methyl-4-(2,6,6-trimethylcyclohex-1-enyl) but-2-en-1-ol (3.67%), and 2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4]non-3-ene (3.57%). Ninety-eight (93.83%) volatile flavor compounds were identified in the essential oil from the Aster yomena Makino. The major compounds were and 3-eicosyne (13.61%), 9,10,12-octadecatrienoic acid (7.8%), ${\alpha}$-caryophyllene alcohol (6.83%), 9-octadecynoic acid (6.03%), and ${\alpha}$-caryophyllene (5.74%). Although the two plants are apparently very similar, the chemical composition of the essential oils was significantly different in quality and quantity. In the case of C. zawadskii var. latilobum Kitamura, the sesquiterpene, valencene was found to be 10.82%, but it was not identified in A. yomena Makino. ${\delta}$-Cadinol appeared higher in C. zawadskii var. latilobum Kitamura than in A. yomena Makino. A clear characteristic of A. yomena Makino essential oil is that it has a high content of caryophyllene derivatives. The ${\alpha}$-caryophyllene alcohol contained in A. yomena Makino was relatively high at 6.83%, although the compound was not identified in C. zawadskii var. latilobum Kitamura. Also ${\alpha}$-caryophyllene was shown to be higher in A. yomena Makino than in C. zawadskii var. latilobum Kitamura.

• 한국약용작물학회지
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• 제19권3호
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• pp.170-176
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• 2011

This study was carried out to investigate whether Chrysanthermum zawadskii var. latilobum Kitamura (C. zawadskii) extracts has an inhibitory effect against the mutagenicity by cigarette smoke condensates (CSC). C. zawadskii was extracted with 70% ethanol and the yield was 18.5%. We further fractioned 70% ethanol extract sequentially to diethylether, chloroform, dichloromethane, and aqueous water, and gained the yield of 17.5%, 5.6%, 5.8%, 32.8% and 35.5%, respectively. In the Ames test, there was no mutagenic effect of crude extract and its solvent fractions up to 2 mg/plate toward Salmonella typhimurium TA 98 with or without S-9 mix metabolic activations. On the contrary, the crude extract showed an inhibitory activity against the mutagenicity of CSC in the presence of S-9 mix metabolic activation. Diethyl ether layer among five solvent fractions showed the highest inhibitory activity. The inhibitory activity of diethyl ether fraction was also increased in a dose-dependent manner and the inhibitory rate was about 97.7% at the concentration of 1 mg/plate. In this study, we conclude that crude extract of C. zawadskii itself is potentially safe for mutagenicity, and the diethyl ether fraction has an inhibitory effect against the mutagenicity of CSC.

• 동의생리병리학회지
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• 제27권6호
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• pp.782-788
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• 2013

Chrysanthemum zawadskii Herbich var. latilobum Kitamura (Compositae), colloquially known "Gujulcho" in Korea, has been used in traditional medicine for the treatment of various diseases, including cough, common cold, bladder-related disorders, gastroenteric disorders, hypertension, and inflammatory diseases, such as pneumonia, bronchitis, pharyngitis, and rheumatoid arthritis (RA) However, the effect of Chrysanthemum zawadskii var. latilobum on breast cancer invasion is unknown. In this study, we investigated the inhibitory effect of Chrysanthemum zawadskii var. latilobum extract (CZE) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced matrix metalloproteinase-9 (MMP-9) expression and cell invasion, as well as the molecular mechanisms involved in MCF-7 cells. CZE were not cytotoxic up to 100 ${\mu}g/ml$ concentration in the MCF-7 cell line. CZE decreased MMP-9 expression. TPA substantially increased NF-${\kappa}B$ DNA binding activity. Pre-treatment with CZE inhibited TPA-stimulated NF-${\kappa}B$ binding activity and NF-${\kappa}B$ related protein expression. To identify invasion ability of MCF-7 cells decreased by CZE, we used martrigel invasion assay. As a result, it is significantly decreased cell invasion. These results indicate that CZE-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the NF-${\kappa}B$ pathway in MCF-7 cells. Chrysanthemum zawadskii var. latilobum may have potential value in restricting breast cancer metastasis.

• 한국자원식물학회지
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• 제19권1호
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• pp.68-75
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• 2006

유망 야생화의 하나인 구절초의 생육에 적합한 배양토를 구명하기 위해 무기재료로서 버미큘라이트, 펄라이트, 암쇄토, 모래, 밭토양을 사용하고 유기물원으로서 피트모스, 부염, 퇴비를 혼합한 배양토를 조성하여 구절초의 생육을 조사한 결과 다음과 같다. 구절초의 생육은 유기물원으로 부엽이 들어간 배양토에서 피트모스나 퇴비를 혼합한 것에 비해 현저히 양호한 것으로 나타났으며 시험 후 배양토의 이화학성 분석 결과 부엽과 피트모스를 사용한 배양토의 화학성은 모든 성분에 있어서 유사한 경향을 나타냈으나 퇴비를 사용한 처리에서는 pH가 ($8.6{\sim}8.9$)로 매우 상승하였고 인산의 함량이 ($237{\sim}1158\;mg/kg$)로 높아져 구절초의 생육을 불량하게 하는 원인이 된 것으로 판단되었다. 피트모스는 화학성분 조사 결과로서는 부엽과 유사한 것으로 나타났으나 양분의 공급 능력에서 부엽에 미치지 못하여 구절초의 생육이 불량하였던 것으로 생각된다. 구절초의 생육에 대한 통계처리 결과를 살펴보면 구절초의 배양토에 있어서 부엽을 유기물원으로 혼합한 상토가 피트모스나 퇴비에 비해 유의성 있는 생육의 증가를 보여 주었다. 부엽을 사용한 VPL, SaVPL, SVPL, UVPL 처리 간에 있어서도 VPL과 UVPL처리가 SaVPL 및 SVPL에 비해 유의성 있는 생육차이를 보였다. 피트모스를 혼합한 처리들과 퇴비를 혼합한 처리들 사이에는 피트모스 처리들이 퇴비에 비해 생육이 양호한 경향을 보였으나 통계적으로는 뚜렷한 경향을 보이지 못하였다.

• 생명과학회지
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• 제16권5호
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• pp.746-749
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• 2006

구절초 전초의 chloroform 분획물로부터 2종의 flavonoid화합물을 분리하여 NMR을 통해서 구조를 확인한 결과, luteolin (1)과 acacetin (2)으로 구조 동정되었다. 이들의 화합물 중에서 luteolin (1)은 구절초에서 처음으로 분리하였다. 분리된 화합물은 sulforhodamine B (SRB) assay법에 따라 인체암세포주인, HCT116 (결장암) , UO-31 (신장암), PC-3 (전립선암) 와 A549 (폐암)등에 대한 in vitro에서의 세포독성을 실험하였다. 그 결과, acacetin (2)이 HCT116 $(IC_{50}\;2.44\;{\mu}g/ml)$과 UO-31 $(IC_{50}\;2.89\;{\mu}g/ml)$에서 유의할만한 세포독성을 나타내었다.

• 한국초지조사료학회지
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• 제27권3호
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• pp.173-182
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• 2007

본 연구는 야생화 초지조성을 위한 잔디형 목초 및 야생화초종의 선정과 혼파조합구성에 대한 기초자료를 탐색하고자 몇 가지 잔디형 목초와 야생화초종을 공시하여 초기생육, 경엽 및 근중, 건물수량, 개화특성 및 기호성 등을 비교분석하였다. 공시초종은 잔디형 목초 5종{Kentucky bluegrass(Midnight), tall fescue(Millennium), perennial ryegrass(Palmer III), redtop(Barricuda) 및 creeping bentgrass(Crenshaw)}과 야생화 11종{Chrysanthemum leucanthemum L., Coreopsis lanceolata L., Rudbeckia bicolor Nutt., Dianthus chinensis L., Chrysanthemum lindicum L., Lotus corniculatus var. Japonicus Regel, Veronica linariaefolia Pallas, Aster koraiensis Nakai., Chrysanthemum zanadskii var. latilobum(Maxim.) Kitamura, Lythrum anceps Makino 및 Iris nertschinskia. Lodd}을 공시하였으며, 충남대학교 농과대학 초지시험포장에서 2005년 1월부터 2006년 12월까지 수행하였다. 공시된 잔디형 목초 중에서 상대적으로 야생화와 혼파가능성이 큰 초종은 Kentucky bluegrass이었으며, Kentucky bluegrass에 비하여 상대적으로 초기생육이 왕성하고 경엽과 근중이 높아 야생화초지를 조성하는 데 유리할 것으로 보이는 야생화는 Chrysanthemum leucanthemum L., Lotus corniculatus var. Japonicus Regel, Chrysanthemum zawadskii var. latilobum (Maxim) Kitamura 및 Lythrum anceps Makino 등으로 조사되었다. 이들 야생화는 꽃색, 개화시기 및 개화지속기간 등이 서로 중복되지 않았으며 가축에 의한 기호성도 대체적으로 양호한 결과를 보였다. 따라서 야생화초지의 조성을 위해서는 잔디형 목초는 야생화와의 경합력이 낮은 초종을, 반대로 야생화는 경합에서 유리한 초종을 선발하되 개화특성이 서로 다른 야생화를 다양화하여 혼파조합을 구성하는 것이 중요하다고 판단된다.

• 생약학회지
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• 제30권1호
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• pp.70-73
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• 1999

Nine sesquiterpene lactones, which were isolated from Hemisteptia lyrata Bunge. Chrysanthemum zawadskii Herbich var. latilobum Kitamura and Chrysanthemum boreale Makino were evaluated for the farnesyl-protein transferase (FPTase) inhibitor, conducted by the scintillation proximity assay (SPA). The angeloylcumambrin B and tigloylcumambrin B inhibited a recombinant rat FPTase with $IC_{50}$ value of $78\;{\mu}g/ml$ $(225\;{\mu}M)$ and $90\;{\mu}g/ml$ $(260 \;{\mu}M)$, respectively.

• Nutrition Research and Practice
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• 제4권2호
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• pp.93-98
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• 2010

Our previous study demonstrated that methanolic extract of Chrysanthemum zawadskii Herbich var. latilobum Kitamura (Compositae) has the potential to induce detoxifying enzymes such as NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) (NQO1, QR) and glutathione S-transferase (GST). In this study we further fractionated methanolic extract of Chrysanthemum zawadskii and investigated the detoxifying enzyme-inducing potential of each fraction. The fraction (CZ-6) shown the highest QR-inducing activity was found to contain (+)-(3S,4S,5R,8S)-(E)-8-acetoxy-4-hydroxy-3-isovaleroyloxy-2-(hexa-2,4-diynyliden)-1,6-dioxaspiro [4,5] decane and increased QR enzyme activity in a dose-dependent manner. Furthermore, CZ-6 fraction caused a dose-dependent enhancement of luciferase activity in HepG2-C8 cells generated by stably transfecting antioxidant response element-luciferase gene construct, suggesting that it induces antioxidant/detoxifying enzymes through antioxidant response element (ARE)-mediated transcriptional activation of the relevant genes. Although CZ-6 fraction failed to induce hepatic QR in mice over the control, it restored QR activity suppressed by $CCl_4$ treatment to the control level. Hepatic injury induced by $CCl_4$ was also slightly protected by pretreatment with CZ-6. In conclusion, although CZ-6 fractionated from methanolic extract of Chrysanthemum zawadskii did not cause a significant QR induction in mice organs such as liver, kidney, and stomach, it showed protective effect from liver damage caused by $CCl_4$.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.358-366
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• 2002

To search for novel cancer preventive agents, we assessed the quinone reductase (QR)-inducing activities of medicinal herb extracts in cultured murine hepatoma cells (hepalclc7 cells). Among 216 herb extracts tested in this study, 8 kinds of herbal extracts were found to induce QR activity in hepalclc7 cells by more than 2-fold when used at the concentration of 25 $\mu\textrm{g}$/$m\ell$. The methanol extracts of Aster koraiensis NK and Pulsatilla koreana Nakai induced QR by 252 and 223 % , respectively, at the concentration of 25 $\mu\textrm{g}$/$m\ell$. Most of the herbal extracts with QR inducing-activity increased the enzyme activity in a typical dose-dependent manner. The QR activity in BP$^{r}$ cl cells was induced move than 50 % by the extracts of Pulsatilla koreana Nakai, Inula helenium, Physalis alkekengi var, francheti (Masters) Makino, Chrysanthemum zawadskii var. latilobum Kitamuva, Auemisia keiskeana Miquel, Chfsanthemum boreale Makino. In conclusion, hlsatilla koreana Nakai, Aster koraiensis N.K, and Chfsanthemum zawadskii var. iatilobum Kitamura, which showed relatively high QR induction, merit further animal study to evaluate their potential as cancer preventive agents.

• Applied Biological Chemistry
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• 제40권1호
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• pp.85-88
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• 1997

Chrysanthemum속(屬) 식물의 성분 및 생리활성물질에 대한 연구의 일환으로, 구절초 꽃의 chloroform 분획물을 재료로 B. subtilis, 및 V. aureus 및 v. parahaemolyticus에 대한 activity-guieded fractionation을 실시하여 항균성 물질 두가지를 얻었다. 항균력실험 결과,화합물I은 $100\;{\mu}g/disk$의 농도에서 세가지균주 모두에 활성이 있었고 화합물 II는 B. subtilis와 V. parahaemolyticus에만 활성이 나타났다. 화합물 I과 화합물 II는 현재 식품보존료로 사용되는 benzoic acid 및 sorbic acid와 비교해볼 때, B. subtilis와 v. perahaemolyticus에 대하여 대략 5배정도 강한 항균력을 나타내었다. $^1H,\;^{13}C\;NMR,\;DEPT,\;^1H-^1H\;COSY,^{13}C-^1H\;COSY$ 및 Mass spectrum 등을 분석한 결과, 화합물 I과 II는 sesquiterpene lactone인 angeloylcumambrin B($C_{20}H_{25}O_5,$ MW=346)와 cumambrin A($C_{17}H_{25}O_5,\;W=346$)로 각각 동정되었으며 이들은 구절초에서는 처음 분리되었다.