Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Protection by Chrysanthemum zawadskii extract from liver damage of mice caused by carbon tetrachloride is maybe mediated by modulation of QR activity

  • Seo, Ji-Yeon (Department of Animal Science and Biotechnology and School of Applied Biosciences, Kyungpook National University) ;
  • Lim, Soon-Sung (Department of Food Science and Nutrition, Hallym University) ;
  • Park, Ji-A (Department of Animal Science and Biotechnology and School of Applied Biosciences, Kyungpook National University) ;
  • Lim, Ji-Sun (Department of Animal Science and Biotechnology and School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Hyo-Jung (Department of Animal Science and Biotechnology and School of Applied Biosciences, Kyungpook National University) ;
  • Kang, Hui-Jung (Department of Human Ecology, KyungSung University) ;
  • YoonPark, Jung-Han (Department of Food Science and Nutrition, Hallym University) ;
  • Kim, Jong-Sang (Department of Animal Science and Biotechnology and School of Applied Biosciences, Kyungpook National University)
  • Received : 2009.11.17
  • Accepted : 2010.02.21
  • Published : 2010.04.28
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Abstract

Our previous study demonstrated that methanolic extract of Chrysanthemum zawadskii Herbich var. latilobum Kitamura (Compositae) has the potential to induce detoxifying enzymes such as NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) (NQO1, QR) and glutathione S-transferase (GST). In this study we further fractionated methanolic extract of Chrysanthemum zawadskii and investigated the detoxifying enzyme-inducing potential of each fraction. The fraction (CZ-6) shown the highest QR-inducing activity was found to contain (+)-(3S,4S,5R,8S)-(E)-8-acetoxy-4-hydroxy-3-isovaleroyloxy-2-(hexa-2,4-diynyliden)-1,6-dioxaspiro [4,5] decane and increased QR enzyme activity in a dose-dependent manner. Furthermore, CZ-6 fraction caused a dose-dependent enhancement of luciferase activity in HepG2-C8 cells generated by stably transfecting antioxidant response element-luciferase gene construct, suggesting that it induces antioxidant/detoxifying enzymes through antioxidant response element (ARE)-mediated transcriptional activation of the relevant genes. Although CZ-6 fraction failed to induce hepatic QR in mice over the control, it restored QR activity suppressed by $CCl_4$ treatment to the control level. Hepatic injury induced by $CCl_4$ was also slightly protected by pretreatment with CZ-6. In conclusion, although CZ-6 fractionated from methanolic extract of Chrysanthemum zawadskii did not cause a significant QR induction in mice organs such as liver, kidney, and stomach, it showed protective effect from liver damage caused by $CCl_4$.

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References

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