• Korean Journal of Plant Resources
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• v.17 no.1
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• pp.43-47
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• 2004

The effect of plant growth regulators and gelling agents for plant regeneration from leaf segment of Chrysanthemum zawadskii ssp. coreanum was investigated. NAA was more effective than BA for plant regeneration. MS medium supplemented with NAA 1 mg/L was the most effective in plant regeneration. The effect of agar and gelite as gelling agent was compared. Agar(0.8%) was more effective than gelite(0.2%) in plant regeneration. Regenerated shoots was successfully increased by shoot grafting in MS medium supplemented with NAA 0.1 mg/L in vitro, and hardened by shoot grafting in artificial soil mix(Peatmoss : Perlite = 1 : 1).

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.5
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• pp.15-23
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• 2021

This study was carried out to suggest an appropriate plant combination by evaluating the growth of flowering ground-cover plants planted with Festuca glauca 'Eljiah Blue' on the roof-top environment. As for the plant materials, Allium senescens and Chrysanthemum coreanum which are shorter than Festuca glauca 'Eljiah Blue' and Sedum takesimense and Agastache rugosa which are taller than Festuca glauca 'Eljiah Blue' were selected. Festuca glauca 'Eljiah Blue' was planted on Conrol, and Festuca glauca 'Eljiah Blue' with Allium senescens (T1), Festuca glauca 'Eljiah Blue' with Sedum takesimense (T2), Festuca glauca 'Eljiah Blue' with Agastache rugosa(T3), and Festuca glauca 'Eljiah Blue' with Chrysanthemum coreanum (T4) were planted in each experimental plot. Plant height and covering rate were measured to evaluate the growth of Festuca glauca 'Eljiah Blue'. Also, relative growth rate (RGR) of plant height, RGR of plant width, and mortality rate of the flowering ground-cover plants were estimated. Plant height and cover rate of Festuca glauca 'Eljiah Blue' was greatest in T3. RGR of plant height was greater in the order of Agastache rugosa, Allium senescens, Chrysanthemum coreanum, and Sedum takesimense. In particular, RGR of plant width was also greatest for Agastache rugosa. Mortality rates of Agastache rugosa and Allium senescens were lowest at 11%. Therefore, based on good growth of Festuca glauca 'Eljiah Blue' planted with Agastache rugosa, these results were suggested as a desirable combination of plant species for rooftop gardening.

• Journal of the Korean Institute of Landscape Architecture
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• v.39 no.1
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• pp.117-123
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• 2011

This study aims to suggest a suitable soil thickness and soil mixture ratio of a green roof system by verifying the growth of Chrysanthemum zawadskii var. coreanum as affected by different green roof systems using rainwater. The experimental planting grounds were made with different soil thicknesses(15cm, 25cm) and soil mixing ratios (SL, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$, $P_4P_4L_2$) and with excellent drought tolerance. Ornamental value Chrysanthemum zawadskii var. coreanum was planted. The change in plant height, green coverage ratio, chlorophyll content, fresh weight, dry weight, and dry T/R ratio of Chrysanthemum zawadskii var. coreanum were investigated from April to October 2009. For 15cm soil thickness, the plant height of Chrysanthemum zawadskii var. coreanum was not significantly different as affected by the soil mixing ratio. However, it was found to be higher in the amended soil mixture, $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ and $P_4P_4L_2$ than in the sandy loam soil, as it was SL overall. For 25cm soil the plant height differences were in order to SL < $P_7P_1L_2$, $P_6P_2L_2$, $P_5P_3L_2$ < $P_4P_4L_2$. The green coverage ratio was observed not to be different by soil mixing ratio with soil thickness of 15cm, but, the lowest green coverage ratio in the SL. In the 25cm soil thickness, the green coverage ratio was 86-89% with a good coverage rate overall. The change in chlorophyll contents with 15cm soil thickness was found to be the highest in the SL treatment and the lowest in the $P_5P_3L_2$ treatment. For 25cm thickness, the highest value was in the $P_4P_4L_2$ and SL, and the lowest in the$P_7P_1L_2$. Fresh weight and dry weight were larger in soil with 25cm thickness. Therefore, the growth of Chrysanthemum zawadskii var. coreanum as affected by a different green roof system for using rainwater was higher in soil with 25cm thickness than 15cm, and in PPL amended soil than in sandy loam.

• Journal of Plant Biology
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• v.12 no.1
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• pp.35-48
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• 1969

This study was made on the taxa Chrysanthemum zawadskii complex that grow in South Korea on the basis of chromosomes, epidermis, pollens and gross morphology. I have found four types of chromosome numbers, 36, 45, 54, and 72 as a polyploidal series. Even though the gross morphology was quite similar almost the same gross morphology, chromosome number was different among the taxa. The taxa of 36 chromosomes present broad and fine lobed leaves which grow separately, broad leafed taxon in the mainland of Korea and the other's in Ullungdo Island which is isolated form the mainland in the East Sea. The taxa of 54 chromosomes are also present in the broad and in the fine lobed leaves. The fine lobed leave taxon grows in central to northern Korea and in the high altitude of mountains. Broad leafed taxon grows in central to southern Korea and comparatively lower altitude of the mountains. The taxon of 72 chromosomes is grown in the high altitude of Mt. Hallasan which is isolated from the mainland of Korea. According to this study of Chrysanthemum zawadskii complex, I have arranged the scientific names, as Chrysanthemum zawadskii subsp. latilobum, subsp. acutilobum, subsp. naktongenese, subsp. lucidum, subsp. coreanum and hybrid between subsp. acutilobum X subsp. latilobum.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.5
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• pp.584-590
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• 2012

Dried $Compositae$ flowers have traditionally been used for the treatment of anti-inflammatory and anti-oxidative stress in Korea. This paper investigates the effects of $Compositae$ extracts on the inhibition of proliferation and apoptosis of human gastric cancer AGS cells, human breast cancer MDA-MB-231 cells, and SK-BR-3 cells. The proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells were determined by MTT assay. Several $Compositae$ extracts inhibited proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells in a dose-dependent manner. To assess the apoptosis of $Compositae$ extracts, the nuclei of MDA-MB-231 cells were stained with DAPI. The presence of chromatin condensation in the $Compositae$ extract-treated cells was detected on a fluorescent microscope (${\times}200$). We conducted Western blot analysis of changes in Bcl-2, Bax, and p53 protein expression levels. Apoptosis by $Chrysanthemum$ $zawadskii$ subsp. coreanum, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ treatment created a decrease in Bcl-2 expression, whereas the expression of Bax and p53 were increased. These results indicate that $Chrysanthemum$ $zawadskii$ $subsp.$ $coreanum$, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ inhibit breast cancer cell growth through the induction of apoptosis.

• Korean Journal of Plant Taxonomy
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• v.39 no.1
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• pp.29-34
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• 2009

The Chrysanthemum zawadskii complex is demarcated from other species by having the white and pink ligulate flowers. Its morphological characters are greatly diversified, so that various classification systems have been suggested. The character of leaf thickness has been mentioned as the characteristic for recognizing some of infra-specific taxa within this complex. In this study, we used longitudinal leaf sections to investigate the leaf thickness and cell number of leaf blades of 13 populations including those of the members of the C. zawadskii complex, as well as 4 populations of the related species of C. boreale and C. indicum. From the result, it was clear that the leaves were thicker in populations of C. boreale, C. indicum and C. zawadskii complex (diminishing in that order), and that the leaves were composed of about 9 cell layers in all populations. Within the C. zawadskii complex, leaf shape and thickness varied among the populations. It was very interesting that the taxa with restricted distribution, like C. zawadskii var. tenuisectum, C. zawadskii var. alpinum, C. zawadskii var. lucidum, and C. zawadskii subsp. coreanum had a thicker leaves than found among widely occurring taxa. From this, leaf thickness is supposed to be an adaptation to the unique habitat of each population.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.7 no.6
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• pp.75-83
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• 2004

Focusing on native plants that have high possibility of being introduced as rooftop material, this study was conducted to investigate extensive and easy-to-manage rooftop garden and to raise the utilization of native plants by verifying their growing response to artificial substrate soil depth and irrigation period. The study was conducted from March to September in 2002. Plants tested included Chrysanthemum zawadskii, Sedium middendorffianum, Thymus quinquecostatus, Allium senescens, and Dianthus superbus. Regarding soil depth, it was 5 cm and 10 cm. Irrigation period was non-irrigation, 1-week, 2-weeks, and 3- weeks, Its result is as follows; 1. In case of Sedum middendorffianum Maxim, mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. 2. In case of Allium senescens L., mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. Therefore, Provided that fertilizing is managed well, it is a plant that can be highly utilized.3. In case of Chrysanthemum zawadskii Herb. Subsp. (Nakai) Y. Lee Stat., the growth of top was lower in 10cm than in 5cm and it grew well in 10cm. When utilizing for rooftop garden, it would be desirable to keep minimum viable soil depth at over 10cm. If there is enough rainfall, soil and soil depth seem to have greater effect on growth than irrigation period does. 4. In case of Diauthus superbus L. var. longicalycinus (Maxim) Williams, rooting rate and growth were better in 10cm than in 5cm. Therefore, it is desirable to keep minimum soil depth at over 10cm. 5. In case of Thymus quinquecostatus Celak, the growth of top and flowering were better in 10cm than in 5cm. Therefore, it seems desirable to have minimum viable soil depth to be over 10cm. In conclusion, the most suitable species for rooftop garden are Sedium middendorffianum and Allium senescens in this experiment. However, Chrysanthemum zwadskii, Thymus quinquecostatus, and Dianthus chinensis also can be utilized greatly when irrigation is managed regularly in artificial mixed soil over 10cm.