Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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induction of Apoptosis in Human Cancer Cells with Compositae Extracts

국화과 추출물의 암세포 증식 억제 효과

  • An, In-Jung (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Kwon, Jung-Ki (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Lee, Jin-Seok (Dept. of Companion and Laboratory Animal Science, Kongju National University) ;
  • Park, Ha-Seung (Yesan Chrysanthemum Experiment Station) ;
  • Kim, Dong-Chan (Yesan Chrysanthemum Experiment Station) ;
  • Choi, Byung-Jun (Yesan Chrysanthemum Experiment Station) ;
  • Lee, Kyu-Min (Dept. of Plant Science and Technology, Sangmyung University) ;
  • Park, Youg-Jin (Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University) ;
  • Jung, Ji-Youn (Dept. of Companion and Laboratory Animal Science, Kongju National University)
  • Received : 2012.02.09
  • Accepted : 2012.04.03
  • Published : 2012.05.31
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Abstract

Dried $Compositae$ flowers have traditionally been used for the treatment of anti-inflammatory and anti-oxidative stress in Korea. This paper investigates the effects of $Compositae$ extracts on the inhibition of proliferation and apoptosis of human gastric cancer AGS cells, human breast cancer MDA-MB-231 cells, and SK-BR-3 cells. The proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells were determined by MTT assay. Several $Compositae$ extracts inhibited proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells in a dose-dependent manner. To assess the apoptosis of $Compositae$ extracts, the nuclei of MDA-MB-231 cells were stained with DAPI. The presence of chromatin condensation in the $Compositae$ extract-treated cells was detected on a fluorescent microscope (${\times}200$). We conducted Western blot analysis of changes in Bcl-2, Bax, and p53 protein expression levels. Apoptosis by $Chrysanthemum$ $zawadskii$ subsp. coreanum, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ treatment created a decrease in Bcl-2 expression, whereas the expression of Bax and p53 were increased. These results indicate that $Chrysanthemum$ $zawadskii$ $subsp.$ $coreanum$, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ inhibit breast cancer cell growth through the induction of apoptosis.

국화과 꽃은 우리나라에서 전통적으로 항염증과 항산화 치료에 사용되었다. 본 연구에서는 국화과 추출물이 인간 위암세포 AGS, 인간 유방암세포 MDA-MB-231과 SK-BR-3 암세포에서 성장을 억제하고 세포자멸사를 유발하는지 확인하였다. AGS, MDA-MB-231 그리고 SK-BR-3 암세포의 성장을 MTT로 측정하였다. 14종의 국화과 추출물을 24시간 동안 50, 100, 200 ${\mu}g/mL$의 농도로 처치하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부, 낙동구절초 전초, 산국 전초 그리고 해국 꽃 추출물에서 암세포의 성장을 농도 의존적으로 억제시켰다. 우리나라 여성에서 가장 많이 발생하는 유방암세포인 MDA-MB-231 암세포에서 세포자멸사를 확인하기 위해 DAPI 염색을 수행하였다. MTT assay에서 암세포를 억제시킨 6종의 국화과 추출물중 3종인 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 처치한 세포에서 핵의 응축이 농도 의존적으로 존재함을 형광현미경으로 확인하였다(${\times}200$). 세포자멸사에 관련된 단백질의 발현을 알아보기 위해서 western blot으로 확인하였다. 한라구절초 전초, 포천구절초 전초, 삼잎국화 지하부 추출물을 25, 50 ${\mu}g/mL$의 농도로 24시간 동안 MDA-MB-231 암세포 처치 후 cell lysate를 얻어 Bcl-2, Bax 그리고 p53의 변화를 관찰하였다. 한라구절초 전초, 포천구절초 전초 그리고 삼잎국화 지하부에서 anti-apoptotic 분자인 Bcl-2 단백질은 감소하고 반대로 pro-apoptotic 분자인 Bax와 p53 단백질은 증가하였다. 결과적으로 한라구절초 전초, 포천구절초 전초 그리고 삼잎국화 지하부 추출물은 유방암 세포의 성장을 억제하고 apoptosis를 유발시키므로 암예방제나 치료제로 개발될 수 있을 것으로 사료된다.

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