• Molecular & Cellular Toxicology
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• 제5권3호
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• pp.216-221
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• 2009

Bisphenol A (BPA) is commonly used in the production of pharmaceutical, industrial, and housing epoxy, as well as polycarbonate plastics. Owing to its extensive use, BPA can contaminate the environment either directly or through derivatives of these products. BPA has been classified as an endocrine disruptor chemicals (EDCs), and the primary toxicity of these EDCs in males involves the induction of reproductive system abnormality. First, in order to evaluate the direct effects on the Y chromosome associated with reproduction, we evaluated Y chromosome abnormalities using a Y chromosome microdeletion detection kit. However, we detected no Yq abnormality as the result of BPA exposure. Secondly, we performed high-density oligonucleotide array-based comparative genome hybridization (CGH) to assess genomic alteration as a component of our toxicity assessment. The results of our data analysis revealed some changes in copy number. Seven observed features were gains or losses in chromosomal DNA (P-value<1.0e-5, average log2 ratio>0.2). Interestingly, 21 probes of chr7:7312289-10272836 (qA1-qA2 in cytoband) were a commonly observed amplification (P-value 3.69e-10). Another region, chr14:4551029-10397399, was also commonly amplified (P-value 2.93e-12, average of log2 ratios in segment>0.3786). These regions include many genes associated with pheromone response, transcription, and signal transduction using ArrayToKegg software. These results help us to understand the molecular mechanisms underlying the reproductive effects induced by BPA.

• 한국약용작물학회지
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• 제13권1호
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• pp.48-51
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• 2005

보호식물이며, 약용식물인 깽깽이풀 (Jeffersonia dubia)을 대상으로 핵형 분석과 McFISH 기법을 이용한 염색체 분석을 수행하여 다음과 같은 결과를 얻었다. 체세포 염색체 수는 2n=2x=12였으며, 2쌍의 중부 염색체 (염색체 1, 3), 2쌍의 차중부 염색체 (염색체 2, 4) 그리고 2쌍의 차단부 염색체 (염색체 5, 6)로 구분되었고, 염색체의 평균 길이는 $1.95{\sim}3.50{\mu}M$이었다. McFISH기법을 이용하여 45S와 5S rDNA의 염색체상의 위치를 확인한 바, 3쌍의 45S rDNA signal은 4번, 5번 그리고 6번 염색체의 단완 말단 부위에서 관찰되었고, 한 쌍의 5S rDNA signal은 2번 염색체의 동원체 부위에서 관찰되었다.

• Clinical and Experimental Reproductive Medicine
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• 제24권3호
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• pp.369-375
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• 1997

Studies were conducted to determine the efficiency of decondensation protocols. Sperm obtained from seven normal donors was immediately washed after liquefaction and then decondensed using the method of West et al. (1989) and my original protocol. My optimized protocol entailed mixing 1 ml aliquots of semen with 4 ml phosphate buffered saline (PBS). Following centrifugation, pellets were resuspended in 1 ml PBS containing 6 mM EDTA. After centrifugation, pellets were resuspended in 1 ml PBS containing 2 mM dithiothreitol at $37^{\circ}C$ for 45 min. Following mixing with 2 ml PBS and centrifugation, pellets were resuspended by vortexing. While vortexing, 5 ml of fixative were gently added. Slide preparation was accomplished using the smear method and it was stored at $4^{\circ}C$. When comparing these protocols, the degree of sperm decondensation and head swelling was monitored by measuring nuclear length, area, perimeter, and degree of roundness using FISH analysis software. Apparent copy number for chromosome 1 and, separately, for the sex chromosomes was determined by FISH using satellite DNA probes for loci DIZ1, DXZ1 and DYZ3. Sperm treated by my decondensation protocol showed significant increases (p<0.05) in length, area, perimeter, and degree of roundness. There was a significant decrease (p<0.05) in the frequency of nuclei displaying no signal but no change in the frequency of nuclei with two signals in samples decondensed by my protocol. My data suggested that decondensation using my original protocol may lower the frequency of cells with spurious "nullisomy" due to hybridization failure without inducing spurious "disomy" resulting from increased distances between split signals.

• 생명과학회지
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• 제17권6호통권86호
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• pp.762-765
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• 2007

Giemsa C-banding방법으로 한국 재래종 찰옥수수의 핵형 및 염색체 상에 존재하는 knob의 수와 위치 등을 확인하고 모식도를 통하여 염색체의 특성을 나타내고자 재래종 찰옥수수 5개의 자식 계통 (inbred stock)을 조사하였다. knob의 수는 6-12개이었고 평균 9.2개이었으며 계통별로 차이가 있었으며, knob을 가지고 있는 염색체도계통별로 차이가 있었다. YS-1의 경우 6번, 7번, 8번 그리고 9번 염색체에서 knob을 가지고 있었는데, MY-1의경우 1번, 2번, 3번, 6번, 「번 그리고 9번 염색체에서 knob을 가지고 있었다. 염색체의 장완과 단완의 비율, 염색체의 상대적 길이 등을 비교해 보기 위하여 YS-1과 MY-1 두 계통을 조사해 본 결과 두 계통 간에 차이가 있었고, 염색체의 상대적 길이는 YS-1이 큰 것으로 나타났다. Giemsa C-banding법은 재래종 찰옥수수의 계통별 특성과 이를 이용한 계통 분류에도 효과적으로 이용할 수 있을 것으로 생각된다.

• 식물분류학회지
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• 제35권1호
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• pp.47-55
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• 2005

백두산 및 만주 일대에 생육하는 17과 26속 29종류 31개 집단의 관속식물에 대하여 어린 꽃봉오리를 채취하고 이를 고정하여 감수분열상을 관찰하여 이들 지역에 생육하는 관속식물의 세포분류학적 특징을 논의하고자 하였다. 백두산 지역에서 채집된 종류는 두메양귀비, 등대시호, 땃두릅나무, 가는다리장구채, 비로용담 등 28 집단에 속하는 26종류이었으며 두만강변에서 채집된 종류는 현삼, 과꽃, 신감채 등 3종류로 나타났다. 본 연구를 통하여 비단쑥은 2n=9II로, Cacalia komaroviana는 2n=30II로, 바위구절초는 2n=27II로, 그리고 좁은잎돌꽃은 2n=11II로 그 염색체의 수가 새롭게 보고되었으며, 개발나물과 현삼의 경우는 새로운 염색체의 수가 보고되었다. 기타 기존의 보고와 염색체 수가 일치하는 종류들 중 도깨비엉겅퀴, 나비나물, Allium strictum 그리고 짚신나물의 경우는 4배체로 나타났으며, 두메양귀비는 6배체로 나타났다. 아울러 오랑캐장구채, 털부처꽃 및 물매화의 경우 2배체로 나타났다.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 1998년도 The Third Asian Fuzzy Systems Symposium
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• pp.634-639
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• 1998

A methodology based on the concept of variable string length GA(VGA) is developed for determining automatically the number of hyperplanes and their appropriate arrangement for modeling the class boundaries of a given training data set in RN. The genetic operators and fitness functionare newly defined to take care of the variability in chromosome length. Experimental results on different artificial and real life data sets are provided.

• 산업기술연구
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• 제22권B호
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• pp.71-78
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• 2002

The objective of this paper is to develop an efficient genetic algorithm (GA) to find a channel assignment method for minimum interference among the channels within reasonable time. The series of specific channel number is used as a representation of chromosome. We use minimum-channel-distance encoding scheme within the same cell to consider cosite channel interference (CSI) when chromosomes are generated. The cell base crossover is also used. This proposed method improves solution quality within limited time.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2004년도 수산관련학회 공동학술대회 발표요지집
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• pp.510-511
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• 2004

A terrestrial cave snail (Gastropoda: Subulinidae), Sinistras chois n. gen., n. sp., from the Gungi-cave in Jeju Island, South Korea represents a monotypic genus, and is proposed as a new genus in the family Subulinidae. Diagnostic features of the genus include a sinistral shell, that has a minute-sized, elongate conic-shaped, apical microsculpture with low tubercles. The chromosome number and karyotype are 2n=56 and 8M+15SM+5ST, respectively.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 춘계 학술대회지
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• pp.72-73
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• 1990

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 춘계 학술대회지
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• pp.70-71
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• 1990