• Environmental Mutagens and Carcinogens
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• 제19권1호
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• pp.28-33
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• 1999

The SNF2 family includes proteins from a variety of species with roles I cellular processes such as transcriptional regulation, recombination and various types of DNA repair. Several proteins with unknown function are also included in this family. Here, we report the cloning and characterization of hrp 2+ gene (helicase related gene from S. pombe) which was isolated by PCR amplication using the conserved domain of SNF2 motifs within the ERCC6 gene which encodes a protein involved in DNA excision repair. The hrp2+ gene was isolated by screening with yeast S. pombe genomic library. The isolated cloned contained 6.5 kb insert DNA. Southern blot analysis confirmed that S. pombe chromosome contains the same DNA as hrp2+ gene and this gene exists as a single copy in S. pombe genome. The 4.7 kb transcript of mRNA was identified by Northern blot. To examined the transcriptional regulation of hrp2+ gene, DNA damaging agents were treated. These results indicated that the hrp2+ gene may not be directly involved in DNA replication, but may be involved in damage response pathway.

• Animal cells and systems
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• 제2권4호
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• pp.435-438
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• 1998

In the present study, we have examined the crossability of four species in the A. jaluense complex to provide additional information on their delimitation and levels of differentiation. Crosses between diploid A. chiisanense and the tetraploid species including A. jaluense s. str., A. triphyllum, and A. pseudoproliferum yielded no fruit- or seed-set, indicating that the former species is reproductively completely isolated from the latter three species. In interspecific crosses between the tetraploid species, combinations involving A. jaluense s. str. resulted in much reduced fruit- and seed-set, indicating that A. jaluense s. str. is more or less reproductively isolated from the other tetraploid species. However, crossing data revealed the absence of reproductive isolation between A. triphyllum and A. pseudoproliferum These results strongly support the relationships that have been previously suggested for the A. jaluense complex on the basis of morphology, chromosome number, and flavonoid chemistry.

• Journal of The Korean Association For Science Education
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• 제14권2호
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• pp.170-183
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• 1994

In this study, in order to find our high school students' misconceptions on genetics and evolution, a free-answer form of questionnaire, specially designed through analysis of high school biology curriculum, was developed and sent to one hundred and eight high school students in Seoul area. Their responses were collected and qualitatively analyzed. Main results of the study can be summarized as follows: 1. Some students misunderstood that aquired chracters are inherited. 2. Several students weren't instructed clearly about gene. For example, they responsed that genes are not material, composed of proteins, exist only in reproductive cells and are not in plant cells at all. 3. Some students confused chromosome with chlorophyll, dorminant (in dorminant character) with excellent. They are caused by similar spells in Korean letter 4. Half students misconceptualized that organisms evolve just when their environment changes for the worse. 5. Many students believed orthogenesis.

• Journal of Aquaculture
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• 제15권4호
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• pp.275-277
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• 2002

To obtain baseline data required for ploidy induction in Silurus asotus, a species with enormous aquacultural potential, histological events related to early embryonic development at 24$\pm$0.5$^{\circ}C$ are described. The histological location of the one-celled embryos indicated that metaphase stage of the first cleavage occurs at 31 min after insemination, when the highest yield can be obtained for the chromosome set manipulation.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제49권5호
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• pp.671-674
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• 2016

We cytogenetically analyzed a triploid King-Nupchi strain of the olive flounder Paralichthys olivaceus to define the simplest, most rapid, and most effective method of ploidy analysis in aquaculture farms. Female triploidy of the flounder King-Nupchi strain was induced by cold shock (3 min post-fertilization at 2-4℃ for 45 min). Triploid induction was confirmed by erythrocyte measurement (nuclear volume, 29.15±2.10 μm³); flow cytometry (2.14±0.03 pg/cell); chromosome count (3N=72); Ag-NOR banding; and silver staining. Silver staining of finned cells obtained using a solid tissue technique was the most effective method of ploidy verification.

• Clinical and Experimental Pediatrics
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• 제53권12호
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• pp.1022-1025
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• 2010

Thanatophoric dysplasia (TD) is a short-limb neonatal dwarfism syndrome that is usually lethal in the perinatal period. It is characterized by shortening of the limbs, severely small thorax, large head with a prominent forehead, macrocephaly, curved femur, and flattened vertebral bodies. These malformations result from the mutation in fibroblast growth factor receptor 3 (FGFR-3) gene which is located on the short arm of chromosome 4. A definite diagnosis should be established by molecular genetic analysis to find out the abnormal mutations in the $FGFR3$ gene. We confirmed by detection of a R248C mutation in the $FGFR3$ gene in DNA analysis.

• Fisheries and Aquatic Sciences
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• 제14권4호
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• pp.429-433
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• 2011

Eggs of Korean rose bitterling Rhodeus uyekii were collected and fertilized to observe temperature-related cleavage rates and mitotic intervals (${\tau}_0$). As the water temperature was increased, the slope of first cleavage frequency with elapsed time after fertilization increased, and approximately 30% of fertilized eggs reached first cleavage frequency at every 15 min. At higher temperatures, eggs developed faster and underwent further identical developmental processes. There were strong, negative correlations between ${\tau}_0$ and water temperatures at all temperatures studied (Y = -1.225X + 70.05, $r^2=0.988$, where Y is ${\tau}_0$ and X is temperature).

• Journal of Microbiology and Biotechnology
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• 제3권4호
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• pp.252-255
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• 1993

Pseudomonas maltophilia N246 carrying on OCT plasmid grew on n-alkanes of 6 to 14 carbon atoms, but not on n-alkanes of more carbon atoms. P. maltophilia strains with and without OCT plasmid could utilize primary alcohols. aldehydes and fatty acids derived from n-alkane. The N246 strain could also utilize monocarboxylic and dicarboxylic acids, and terminal branched dimethyloctane. Unlike the genes of alcohol dehydrogenase and aldehyde dehydrogenase which were located on both the chromosome and the OCT plasmid, genes for the alkane hydroxylase components were located only on the OCT plasmid in P. maltophilia N246.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 한국정밀공학회 1996년도 추계학술대회 논문집
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• pp.548-554
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• 1996

A new method to locate modular fixtures using an optimization technique is proposed. The optimal fixture arrangement is derived to minimize the elastic deformation of a workpiece. That is, a fixture arrangement is regarded better if it minimized the elastic deformation of the workpiece while fixing a workpart of course. In this approach, the workpiece is projected into two dimensional domain to simplify the 3-dimensional fixture arrangement problem into 2-dimensional one. Thus the problem is reduced to find the optimal positions of one horizontal clamp and three locators which minimize the total deformation of the workpiece and the design variables are the location of the contact points between the boundary of the workpiece and the 4-fixels. The Genetic Algorithm is used for the optimization by mapping each design variables to a gene of a chromosome. The fitness value is the total strain energy of the workpiece calculated by the fin element analysis.

• KOREAN JOURNAL OF CROP SCIENCE
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• 제46권4호
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• pp.328-333
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• 2001

Allium is one of the largest genera, which has more than 700 species. PCR by URP (universal rice primer) primers was carried out to get phylogenetic information on 26 species, 62 accessions of subgenus Rhizirideum. The accessions were divided into seven groups at 0.76 similarity level. A. tuberosum (Chinese chives) and A. ramosum represented high similarity of 0.91. A. montanum, A. nutans, A. senescens, A. libani, A. odorum, A. austrosibiricum, and A. narcissiflorium grouped at 0.80 similarity. Some of the wild species, such as A. prostratum, A. polyrhizum, A. odorum, and A. mongolicum, showed different band patterns according to polyploidy, occurrence of B-chromosome, collection site, and origin.