• Title/Summary/Keyword: Chromosome 2

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Studies on the Chromosome Types of Ginkgo Species (은행(銀杏)나무류(類) 염색체(染色體)에 관(關)한 연구(硏究))

  • Kim, Su In
    • Journal of Korean Society of Forest Science
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    • v.84 no.2
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    • pp.131-144
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    • 1995
  • To determine the structure of chromosome and to identify the sex chromosome of Ginkgo biloba and G. biloba var. fastigiata, the samples were obtained from root tips of trees growing in seven different provinences. The results are as follows. The basic number of somatic chromosomes was 2n=24. The range of a relative length of long chromosome was between $14.88{\sim}11.18{\mu}m$ and that of short chromosome was $8.11{\sim}6.24{\mu}m$. The chromosome sets were composed with one long pairs of m type and 11 short pairs of sm or st type. These short pairs showed the continuous descending in length. There was a satellite on the short arm of the Longest chromosome pair, and were satellites of the one or both long arm of 7th or 8th chromosome pair which were sm or st type, or the shortest st type chromosome pair. Sometimes, a satellite on the short arm of the longest chromosome pairs of Ginkgo biloba was double satellite, but that of G. biloba var. fastigiata was not. Karyotype was $2n=24=2^{2s}A^m+2B^{st\;or\;sm}+2C^{st}+2D^{st}+2E^{st}+2F^{st\;or\;sm}+2G^{sm}+2^{2s}H^{sm}\;or\;(^{1s}H^{sm}+H^{sm})+2I^{st}+2J^{st}+2K^{st}+2^{2s}L^{st}\;or\;(^{1s}L^{st}+L^{st})$. The male and female trees were not apparently distinguished by the chromsome structures. However the differences between the satellites could be used to identify the male and females. The male tree has double satellite on short arm of a longest chromosome pairs and females' has not. Also female trees have a satellite on a short chromosome more frequently than male trees.

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Some Chromosome Alteranations in the Cultured Chinese Hamster Cells Treated by Steroids (Steroid 물질처리를 받은 Chinese hamster 세포에 있어서의 염색체 이상)

  • 강영선
    • The Korean Journal of Zoology
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    • v.6 no.2
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    • pp.21-27
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    • 1963
  • This study is concerned with alterations in chromosomes (numbers and morphology) when the culture of Chinese hamster cells (FAF-28 strain) was treated by steroids, testosterone and DOC. 1. In 200 cells of normal untreated cells as control population the chromosome of stemline was decided as which was contained in 158 cells ; that is , in 79 percent of the population. The average chromosome number in above 20 cells observed was calculated as 23.95 with minimum limit at 20 and maximum limit at 70. 2. Many different chromosome numbers, ranging from 19 to 352 were observed in the 200 cells treated by testosterone. The diploid number of 22 showed the peak of variation curve was counted in 71 cells (35.5%) and an average chromosome number of stemline was 22 which was counted in 74 cells (37%). While all of the chromosome number of stemline was 22 which was counted in 74 cells (37%). While all of the chromosome numbers in the 200 cells observed ranged from 20 to 181 , an average chromosome number was also found to be 30.09. 4. The chromosome component in the cultured normal FAF-28 cells with 22 diploid chromosomeswas as follows ; 9a) 2 paris were long and metacentric (LM), (b) 3 pairs were medium length and metacentric (MM), (c) 3 pairs were small and subtelocentric (SS) and (d) 3 pairs were small and metacentric (SM). 5. The twenty cells with 44 chromosomes were selected at random from each cell population treated with testosterone and DOC , so that chromosome idiogram and morphology could be studies. In the twenty cells of the testosterone treated population the average ratio of above four groups, LM ; MM;Ss:SM, was found to be 8.6 : 10.8:13.5:10.7. On the other hand, the average ratio in the same number of cells of the DOC treated one was 7.7 :11.4:12.5:12.7. 6. The five types of the altered chromosomes morphologically in the hundred cells selected at random from each cell population treated by testosterone and DOC were observed (Type I-V). The thirty-one altered chromosomes were found to be in the testosterone treated cell population and the sixteen in DOC treated.

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Karyotype analysis of Neodiplostomum seoulense (서울주걱흡충 염색체 핵형 분석)

  • Gab-Man PARK;Soo-Ung LEE;Hyun-Young PARK;Sun HUH
    • Parasites, Hosts and Diseases
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    • v.36 no.4
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    • pp.277-279
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    • 1998
  • A karyotype analysis of the chromosome of Neodiplostomum seoulense, one of causative agents of human intestinal trematodiasis, was done from the gonad tissue by the squashing method. The chromosome number was n=10 and 2n=20. Chromosome length was $1.30-4.0{\;}\mu\textrm{m}$. Chromosome pairs in the complement consisted of two pairs of metacentric, five pairs of submetacentri$cs_telocentric and three pairs of telocentric chromosomes. These data were comparable with those of other intestinal trematodes.es.

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Variation of Univariate Flow Karyotypes and Chromosomal DNA Contents in Maize (Zea mays L.)

  • Lee, Jai-Heon;Lee, Myoung-Hoon;Kim, Kyung-Je
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.43 no.2
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    • pp.128-133
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    • 1998
  • Analyses of now karyotypes using different maize (Zea mays L.) inbred lines have been performed. The accumulation and isolation of high quality and quantity metaphase chromosomes from root tips can be achieved from many kinds of maize lines. The chromosome suspensions were prepared by a simple slicing method from synchronized maize root tips and analyzed with a now cytometry. The variations of experimental now karyotypes were detected among inbred lines in terms of the positions and/or the numbers of chromosome peaks. The 2C DNA amount among 8 inbred lines ranged from 5.09 to 5.52 pg. The variability of DNA content in maize chromosome 1 was 9.1 % ranging from 0.685 to 0.747 pg. The selection of appropriate maize lines is critical for sorting specific single chromosome types. At least five different chromosome types can be discriminated and sorted from five maize lines.

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Chromosome Number Evolution in Cirsium Mill. and Carddus L. (Asteraceae)

  • Kang, Seong-Yeon;Jang, Tae-Soo
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.25-25
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    • 2019
  • Chromosome numbers and karyotypes in flowering plants have been considered to be prominent features in taxonomic and evolutionary context. Despite the increasing numbers of cytological studies in Asteraceae, karyotype analysis of Cirsium Mill. and Carddus L. in Korean population have not been performed carefully. In this study, the chromosome numbers and karyotype analysis of all eight species of the genus Cirsium Mill. and one species of Carddus L. were analyzed. While the chromosome number in Carduus crispus L. was diploid (2n = 2x = 18 or 18+2Bs) with x = 9 as the base chromosome number, all seven species of Cirsium were diploid with x = 17 except for Cirsium lineare (Thunb.) Sch. Bip. (x = 14). The chromosome number in C. pendulum Fisch. ex DC. presented 2n = 2x = 34 from two populations and C. lineare exhibited 2n = 2x = 28 from one population. Aneuploidy was occasionally found in C. japonicum Fisch. ex DC. var. spinossinum Kitam. (2n = 2x = 34, 35, 36), C. rhinoceros (H. $L{\acute{e}}v.$ & Vaniot) Nakai (2n = 2x = 32, 34), C. setidens (Dunn) Nakai (2n = 2x = 30, 31, 32) and C. vlassovianum Fisch. ex DC. (2n = 2x = 31, 32). While Cirsium japonicum Fisch. ex DC. var. japonicum possessed several B-chromosomes (2n = 2x = 34, 35, 36), polyploidy was only encountered in Cirsium nipponicum (Maxim.) Makino. (2n = 4x = 68) from two populations in Ulleung Island. The present cytological data might be contributed to the taxonomic and evolutionary studies in the genus Cirsium.

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Karyotype Analysis of Anemarrhena asphodeloides Bunge (지모의 핵형 분석)

  • Kim, Soo-Young;Koo, Dal-Hoe;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.2
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    • pp.144-146
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    • 2002
  • Cytogenetic analysis of Anemarrhena ashodeloides BUNGE, which is one of medicinal plants belonging to Haemodoraceae was carried out using Feulgen staining. The somatic metaphase chromosome number was identified to 2n=22 (x=11) and the size of chromosomes ranges from $1.27-3.80\;{\mu}m$. Three pairs of chromosomes were relatively long in total length and the others were short. The karyotype was bimodal in chromosome length and arm ratios. The chromosome complement comprise eight pairs of metacentric (chromosome 2, 3, 6, 7, 8, 9, 10, and 11), two pairs of submetacentric (chromosome 4 and 5), and one pair of subtelocentric (chromosome 1).

Chromosome Studies on the Cultured Uterine Carcinoma Cells (배양한 子宮癌세포의 염색체에 관한 연구)

  • Kang, Yung Sun;Kim, Suk Whan;Lee, Chung Keel
    • The Korean Journal of Zoology
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    • v.13 no.1
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    • pp.29-33
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    • 1970
  • The conclusions established in the present study on the chromosomes in vitro of the uterine carcinomas of Korean women are as follows: 1. The pattern of the distribution of chromosome number in uterine carcinoma cells was quite different from that of normal cells, and modal number of the chromosome was 45 and 46. 2. The frequency of diplochromosomes was 0.053 per cell (5.3%) and that of chromosome aberration was 0.16 per cell (16%), which are significantly higher than each of normal cells. In chromosome aberration types, chromatid and isochromatid deletions (chromatid type) and dicentric (chromosome type) were observed. 3. Idiogram analysis showed a tendency that the number of chromosomes belonging to group F increased while that of chromosomes in groups B and E decreased in total. The number of chromosomes in groups C and G in the hypodiploidy cells decreased, but it increased in the hyperdiploidy cells.

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A taxonomic study of Korean Artemisia L. using somatic chromosome numbers (한국산 쑥속의 체세포 염색체수에 의한 분류학적 연구)

  • Park, Myung Soon;Jang, Jin;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.39 no.4
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    • pp.247-253
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    • 2009
  • Somatic chromosome numbers for 20 taxa of Korean Artemisia L. were investigated for the purpose of classification. Somatic chromosome numbers of treated taxa were 2n = 16, 18, 34, 36, 50, 52, 54, and therefore their basic chromosome numbers were x = 8, 9, 10, 13, 17. The chromosome number of A. japonica var. angustissima is being reported for the first time in this study. The chromosome numbers of 13 taxa were the same as in previous reports; A. capillaris (2n = 18), A. japonica var. hallaisanensis (2n = 36), A. japonica subsp. littoricola (2n = 36), A. annua (2n = 18), A. carvifolia (2n = 18), A. fukudo (2n = 16), A. keiskeana (2n = 18), A. stolonifera (2n = 36), A. sylvatica(2n = 16), A. selengensis (2n = 36), A. montana (2n = 52), A. lancea (2n = 16), A. sieversiana (2n = 18); however, the chromosome numbers of 6 taxa were different; A. japonica var. japonica (2n = 18, 36 vs 2n = 36), A. sacrorum (2n = 18, 54 vs 2n = 54), A. rubripes (2n = 16, 34 vs 2n = 16), A. indica (2n = 34, 36 vs 2n = 34), A. codonocephala (2n = 18, 50, 54 vs 2n = 50), A. argyi (2n = 34, 36, 50 vs 2n =34). The somatic chromosome numbers of Korean Artemisia are thought to be good characteristics for classifying some taxa such as A. japonica var. japonica, A. sacrorum, A. codonocephala, A. argyi, A. montana, A. sylvatica.

Somatic chromosome numbers of four Eleocharis taxa from Korean populations (Cyperaceae)

  • Kyong-Sook CHUNG;Chang Shook LEE;Jung-Hyun KIM
    • Korean Journal of Plant Taxonomy
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    • v.52 no.4
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    • pp.214-218
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    • 2022
  • Eleocharis R. Br. (Cyperaceae) is characterized by unbranched culms, one terminal spikelet, bisexual flowers, and bristly perianths, occurring mainly in circumboreal marshes and wetlands. There are about 250 species worldwide, and 15 taxa in Korea have been recognized. Chromosomes in Cyperaceae are known to be holocentric, missing constricted centromeres during cell division. For the first time, we report the chromosome counts of Eleocharis from Korean populations of E. attenuata f. laeviseta (Nakai) H. Hara (2n = 20); E. parvula (Roem. & Schult.) Link ex Bluff, Nees & Schauer (2n = 10); E. ussuriensis Zinserl. (2n = 16); and E. valleculosa var. setosa Ohwi (2n = 16). The populations exhibit variations in their chromosome sizes from ca.1.7 ㎛ to ca. 6 ㎛, categorized as gradient and bimodal karyotypes. The karyotypes observed are congruent with previous phylogenetic groups proposed. To clarify the traditional and phylogenetic classification of Korean Eleocharis, further taxonomic and chromosomal investigations with additional taxon sampling efforts are required.

Chromosome Study on the Oxyloma hirasei ( Gastropoda : Succineidae ) in Korea (한국산 뾰족쨈물우렁이 ( Oxyloma hirasei ) 의 염색체 연구)

  • 박갑만;김재진
    • The Korean Journal of Malacology
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    • v.13 no.2
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    • pp.125-130
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    • 1997
  • The mitotic and meiotic chromosomes of Succineidae snail one species, Oxyloma hirasei(Pilsbry), were investigated by mians of air-drying mithod. The diploid chromosome numbers were n=18, 2n=36. Chromosome complements of this species consist of seven pairs of metacentrics and 11 pairs of submetacentric chromosomes. Spermatogonial metaphase chromosomes range in length from 4.80${\mu}{\textrm}{m}$ for the largest pair to 1.44${\mu}{\textrm}{m}$ for the smallest pair.

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