• 미생물학회지
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• 제53권4호
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• pp.351-353
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• 2017

토마토 뿌리에서 분리한 Chryseobacterium sp. T16E-39 균주는 식물생육촉진과 역병, 시들음병에 대한 억제효과가 있었다. 이 균주의 유전체 염기서열은 4,873,888 염기쌍이었으며 G + C 함량은 35.22%이었다. 이 유전체는 4,289개 단백질 유전자, 15개 rRNA 유전자, 71개 tRNA 유전자를 포함하였다. T16E-39 균주의 유전체에서 인산가용화, 식물호르몬 조절, 항산화 활성, 키틴 분해, 제9형 분비시스템에 관여하는 유전자를 확인하였으며, 이들 유전자는 식물의 생육을 촉진하고 병발생을 억제하는 기작과 관련되어 있을 것으로 판단된다.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.90-90
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• 2003

Circling mice were recorded to display profound deafness and a head-tossing and bidirectional circling behavior, showing an autosomal recessive mode of inheritance. In addition, the histological examination of inner ears revealed that the region around organ of Corti, spiral ganglion neurons and outer hair cells showed definite abnormality. On the other hand, a genetic linkage map was constructed in an intraspecific backcross between cir and C57BL/6J mice. The cir gene was mapped to a region between D9Mitl16/D9Mit15 and D9Mit38 on the mouse chromosome 9. Estimated distances between cir and D9Mitl16, and between cir and D9Mit38 are 0.70 $\pm$ 0.40 and 0.23 $\pm$ 0.23 cM, respectively. The markers in order was defined as follows: centromere-D9Mit182- D9Mit51/ D9Mit79/ D9Mit310- D9Mit212/ D9Mit184- D9Mit116/ D9Mit15- cir- D9Mit38- D9Mit20- D9Mit243- D9Mit16- D9Mit55/ D9Mit125- D9Mit281 Based on genetic mapping, we constructed for a YAC contig across cir region. They covered the entire region or cir and cir gene was located on between the lactotransferrin (ltf) and the macrotubule-associated protein (map4). It is known that sr gene is localized in 64cM of mouse chromosome 9. The two mouse were found to be allelic by complementation test. Recently the spinner mouse has been mapped to our cir region, and tmie gene were elucidated. And further study will be needed in circling mouse to prove tmie gene mutaiton.

• 한국양식학회지
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• 제8권2호
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• pp.141-148
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• 1995

무지개송어 전 암컷 3배체 조기난 생산을 위해 1994년 9월 30일부터 3년생 암컷 친어에 대하여 광주기를 단일 처리하여 조기 산란 및 성전환 유도와 고온 처리에 의한 염색체 조작을 실시하였다. 광주기 조절이 시작된지 1개월째 170마리의 암컷으로부터 340,000개의 난을 얻었다. 그러나, 부화율에 있어서는 자연 상태의 산란기때 부화율에 미치지 못하는 $40\~55\%$ 범위로 평균 $51.3\pm4.2\%$였다. (P<0.05). 수컷으로의 성전환을 위해 $16.5^{\circ}C$의 사육 수온 조건하에서 $17\alpha-methyltestosterone$을 사료 1kg당 3mg 섞어 첫 먹이 공급기부터 55일간 공급하여 성전환율을 조사한 결과 전부 수컷으로 판명되어 $16.5^{\circ}C$의 조건하에서도 성전환이 효율적으로 이루어짐을 알 수 있었다. $13.5^{\circ}C$의 사육수온 조건하에서 3배체를 생산하기 위해 최초 처리 시간 및 고온 처리 기간에 대해 다양한 조건으로 처리한 결과 발안기까지의 생존율에 있어 $15.0\~88.2\%$ 범위로 나타났다. 이때 3배체 유도율은 $36.7\~100\%$ 범위로 다양하였다.

• KSBB Journal
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• 제26권3호
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• pp.199-205
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• 2011

Using tetrameric ${\beta}$-galactosidase as a model protein, anchoring motives were screened in Bacillus subtilis spore display system. Eleven spore coat proteins were selected considering their expression levels and the location in the spore coat layer. After chromosomal single-copy homologous integration in the amyE site of Bacillus subtilis chromosome, cotE and cotG were chosen as possible spore surface anchoring motives with their higher whole cell ${\beta}$-galactosidase activity. PAGE and Wester blot of extracted fraction of outer layer of purified spore, which express CotE-LacZ or CotG-LacZ fusion verified the existence of exact size of fusion protein and its location in outer coat layer of purified spore. ${\beta}$-galactosidase activity of spore with CotE-LacZ or CotG-LacZ fusion reached its highest value around 16~20 h of culture time in terms of whole cell and purified spore. After intensive spore purification with lysozyme treatment and renografin treatment, spore of BJH135, which expresses CotE-LacZ, retained only 1~2% of its whole cell ${\beta}$-galactosidase activity. Whereas spore of BJH136, which has cotG-lacZ cassette in the chromosome, retained 10~15% of its whole cell ${\beta}$-galactosidase activity, proving minor perturbation of CotG-LacZ, when incorporated in the spore coat layer of Bacillus subtilis compared to CotE-LacZ. Usage of Bacillus subtilis WB700, of which 7 proteases are knocked-out and thereby resulting in 99.7% decrease in protease activity of the host, did not prevent the proteolytic degradation of spore surface expressed CotG-LacZ fusion protein.

• 한국발생생물학회지:발생과생식
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• 제16권4호
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• pp.321-327
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• 2012

Korean bullhead (Pseudobagrus fulvidraco) was collected from the Kum River areas of Kangkyung-eup, Nonsan city, Chungcheongnam-do, Korea, from April to June, 2012 and was fertilized in order to observe egg development and temperature-related cleavage rates and mitotic intervals (${\tau}_0$). The fertilized eggs were separative, demersal and light yellowish with $1.5{\pm}0.06mm$ in diameter, and did not contain oil globules. The first cleavage stages were 90 min, 80 min, 60 min and 50 min at $21^{\circ}C$, $24^{\circ}C$, $27^{\circ}C$ and $30^{\circ}C$, respectively. At higher temperatures, eggs developed faster and underwent further identical development. For Korean bullhead, ${\tau}_0$ were $33.4{\pm}2.08$ min at $21^{\circ}C$, $31.5{\pm}3.06$ min at $24^{\circ}C$, $28.1{\pm}2.11$ min at $27^{\circ}C$ and $26.4{\pm}3.35$ min at $30^{\circ}C$. There were strong negative correlations between the $\tau_0$ and water temperatures at all points studied (Y=-1.13X+58.15, $R^2$=0.98, n=30, where Y is ${\tau}_0$ and X is temperature). The results obtained in this work will be helpful for chromosome manipulation by use of cleavage frequency data and ${\tau}_0$ data in Korean bullhead.

• 원예과학기술지
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• 제16권3호
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• pp.361-363
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• 1998

온실에서 춘란(春蘭)과 다른 공시 난과식물을 분식(盆植)하고, 인위적인 타가수분을 실시하여 교배친화성을 검정한 결과, 춘란은 자가수분은 물론 Cym. ensifolium, Cym. kanran, Cym. sinense, Cym. sinense for. albo-jucundissimum, Cym. 'Crystal Cherry Angel', Cym. 'Anmitsu Hime'와 교배친화성을 보였으나 Cym. faberi, Cym. aloifolium, Cym. 'Husky Honey', Dendrobium chrysotoxum, Phalaenopsis spp.는 교배친화성을 나타내지 않았다. RAPD 분석결과, 춘란과 동일한 염색체수를 가진 Cym. faberi, Cym. aloifolium, Cym. 'Husky Honey'는 교배친화성을 보인 다른 공시 Cymbidium들보다 유전적인 근연관계가 멀었으며, 염색체수가 다른 Dendrobium chrysotoxum와 Phalaenopsis spp.는 상대적인 근연관계가 훨씬 먼 것으로 나타났다.

• 대한임상검사과학회지
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• 제40권2호
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• pp.98-105
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• 2008

Colorectal carcinoma from various cancers is fourth ranked occurred to Korean. Due to western dietary life, this cancer has been increased continuously. Therefore, the further study will be needed to find a candidate gene involved in the development and progression of colorectal carcinoma as well as to diagnose and treatment helpfully. The purpose of this study was designed to find a carcinogenesis gene using microsatellite marker on chromosomes 7th and 20th from 30 colon cancer patients. The amplification was investigated in order of D20S97 57% (17/30), D20S101 57% (17/30), D20S119 53% (16/30), D7S483 50% (15/30), D7S495 47% (14/30), D7S498 47% (14/30). The genetic mutation pattern depends on loci of colorectal carcinoma was shown highly amplified with 3.77 from colon cancer than with 2.08 from right colorectal carcinoma (P<0.018). The genetic mutation with lymph nodes was investigated higher with 4.13 at metastasized group than with 1.93 at non-metastasized group (P<0.001). There was no difference at comparison between histological classfication and serological CEA increase as well as on genetic mutated pattern depends on disease stage. It is suggested that the amplification on chromosomes 7q and 20q determines a pivotal role from first stage to metastasis cancer and also functions as an useful marker on diagnosis and treatment of colorectal carcinoma patients as well as follow-up checkup. Recently, the diagnosis and study using genetic analyzer are necessary for efficient application. Fortunately, several university hospitals run this genetic analyzer currently so it is expected that this method makes full use of clinical application.

• Neonatal Medicine
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• 제16권1호
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• pp.71-75
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• 2009

9번 염색체 장완의 중복은 거의 드문 형태의 염색체 이상이며, 특징적인 얼굴형태와 손가락 형태, 정신지체 등이 나타나는 것으로 알려져 있다. 얼굴 형태는 정상이었으나 선천성 심장기형과 수신증, 음낭 탈장이 동반된 미숙아에게서 46,X,Y,dup(9)(q21.2q22.1)를 확인하였고, 표현형이 정상인 환아의 아버지에게서 유래된 것으로 생각되어진 예를 경험하였기에 보고하는 바이다.

• 한국발생생물학회지:발생과생식
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• 제15권3호
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• pp.231-237
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• 2011

The objective of this study was to elucidate the dynamics of microtubules in post-ovulatory aging in vivo and in vitro of mouse oocytes. The fresh ovulated oocytes were obtained from oviducts of superovulated female ICR mice at 16 hours after hCG injection. The post-ovulatory aged oocytes were collected at 24 and 48 hours after hCG injection from in vivo and in vitro, respectively. Immunocytochemistry was performed on ${\beta}$-tubulin and acetylated ${\alpha}$-tubulin. The microtubules were localized in the spindle assembly, which was barrel-shaped or slightly pointed at its poles and located peripherally in the fresh ovulated oocytes. The frequency of misaligned metaphase chromosomes were significantly increased in post-ovulatory aged oocytes after 48 hours of hCG injection. The spindle length and width of post-ovulatory aged oocytes were significantly different from those of fresh ovulated oocytes, respectively. The staining intensity of acetylated ${\alpha}$-tubulin showed stronger in post-ovulatory aged oocytes than that in the fresh ovulated oocytes. In the aged oocytes, the spindles had moved towards the center of the oocytes from their original peripheral position and elongated, compared with the fresh ovulated oocytes. Microtubule organizing centers were formed and observed in the cytoplasm of the aged oocytes. On the contrary, it was not observed in the fresh ovulated oocytes. The alteration of spindle formation and chromosomes alignment substantiates the poor development and the increase of disorders from the post-ovulatory aged oocytes. It might be important to fertilize on time in ovulated oocytes for the developmental competence of embryos with normal karyotypes.

• Journal of Microbiology and Biotechnology
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• 제16권11호
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• pp.1799-1808
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• 2006

A food-grade integration vector based on site-specific recombination was constructed. The 5.7-kb vector, pIMA20, contained an integrase gene and a phage attachment site originating from bacteriophage A2, with the ${\alpha}$-galactosidase gene from Lactobacillus plantarum KCTC 3104 as a selection marker. pIMA20 was also equipped with a controllable promoter of nisA ($P_{nisA}$) and a signal peptide-encoding sequence of usp45 ($SP_{usp45}$) for the production and secretion of foreign proteins. pIMA20 and its derivatives mediated site-specific integration into the attB-like site on the Lactococcus lactis NZ9800 chromosome. The vector-integrated recombinant lactococci were easily detected by the appearance of blue colonies on a medium containing $X-{\alpha}-gal$ and also by their ability to grow on a medium containing melibiose as the sole carbon source. Recombinant lactococci maintained these traits in the absence of selection pressure during 100 generations. The ${\alpha}-amylase$ gene from Bacillus licheniformis, lacking a signal peptide-encoding. sequence, was inserted downstream of $P_{nisA}\;and\;SP_{usp45}$ in pIMA20, and the plasmid was integrated into the L. lactis chromosome. ${\alpha}-Amylase$ was successfully produced and secreted by the recombinant L. lactis, controlled by the addition and concentration of nisin.