• Korean Journal of Microbiology
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• v.29 no.4
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• pp.243-249
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• 1991

The chromatophore from the chemotrophically grown facultative anaerobic photosynthetic bacterium, Rhodopseudomonas gelatinosa ATCC 17013 was isolated through stepwise sucrose gradient centrifugation. The isolated chromatophore showed high activities of the cytochrome $bc_{1}$ complex and cytochrome c oxidase. The activity of cytochrome $bc_{1}$ complex was completely inhibited by .5$\mu$M antimycin A,10$\mu$M myxothiazol, and that of cytochrome c oxidase was completely inhibited by .$50\mu$M KCM and $100\mu$M $NaN_{3}$but not inhibited by carbon monoxie. The activity of cytochrome c oxidase of th chromatophore was increased by addition of ionophores or protonophores. The reduced-oxidised difference sspectrum of cytochrome $bc_{1}$ complex isolated by affivity chromatography showed the absorption maxima at 553 nm(shoulder at 547 nm), 520 nm, and 418.5 nm, on the other hand, that of cytochrome c oxidase showed .alpha., .betha. and soret peaks at 554 nm, 523 nm, and 421 nm, respectively. The cytochrome c oxidase from chemotrophically grown Rhodopseudomonas gelatinosa seems to be a b-type cytochrome c oxidase.

• Journal of Microbiology and Biotechnology
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• v.27 no.12
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• pp.2173-2179
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• 2017

The intracytoplasmic membrane of Rhodobacter sphaeroides readily vesiculates when cells are lysed. The resulting chromatophore membrane vesicle (CMV) contains the photosynthetic machineries to synthesize ATP by ATPase. The light-dependent ATPase activity of CMV was lowered in the presence of $O_2$, but the activity increased to the level observed under anaerobic condition when the reaction mixture was supplemented with ascorbic acid (${\geq}0.5mM$). Cell lysis in the presence of biotinyl cap phospholipid (bcp) resulted in the incorporation of bcp into the membrane to form biotinylated CMV (bCMV), which binds to streptavidin resin at a ratio of approximately $24{\mu}g$ bacteriochlorophyll a/ml resin. The ATPase activity of CMV was not affected by biotinylation, but approximately 30% of the activity was lost by immobilization to resin. Interestingly, the remaining 70% of ATPase activity stayed constant during 7-day storage at $4^{\circ}C$. On the contrary, the ATPase activity of bCMV without immobilization gradually decreased to approximately 40% of the initial level in the same comparison. Thus, the ATPase activity of CMV is sustainable after immobilization, and the immobilized bCMV can be used repeatedly as an ATP generator.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2001.07a
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• pp.287-292
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• 2001

• Journal of Animal Environmental Science
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• v.7 no.2
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• pp.103-110
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• 2001

This study conducted to monitor decomposition process of the charomatophore pollution solution of an animal slurry by using a CCD camera. After the solution was put into test tube, the images(R, G, B, H, L, S) values of the solution were measured by the imgae processing system, and those of it\`s optical density were measured for three hours to be decomposed by microscopic organism. The values of measured for three hours to be decomposed by microscopic organism. The values of measured images(R, G, B, H, L, S) were analysed and compared with those of the optical density. Some of the results are as follows. 1. High correlation coefficients, which analyzed by using data on linear equations, were 0.9557 and 0.9672. They were decreased regularly in this R-value experiment of RGB level. The microscopic organism in this experiment was effective for decomposition of the red charomatophore pollution solution. 2. The values of all correlation coefficients from relationship between RGB-value and optical density were more than 0.95 except H-values. RGB-values, which were average values of summed R, G, B values, had correlation coefficients of 0.9863, 0.9937. These results showed so good relationship that decomposition process of charomatophore pollution solution could be monitored by a image processing system.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.8 no.4
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• pp.225-233
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• 1975

Morphological changes of early post-larval and young stages of Chasmichthys dolichognathus HILGEnDORF (Family Gobiidae) have been studied based on the samples of 953 individuals collected in June 1968, July 1969 and July 1971. Particular emphasis was paid on the development of fin rays, chromatophore patterns and ventral fins. The primordial fin rays of the first dorsal fin appear in the post-larvae of around 8.0 mm in total length, and dorsal fin fully develops in the larvae of around 9.2 mm. In the early young stages of 17.0 mm in total length fin rays have completely developed. According to chromatophore patterns the larvae are grouped into three successive groups. The larvae at the early stages of 6.3-14.2 mm have melanophores on the whole dorsal surface, the posterior ventro-lateral part of the tail and the basal part of the caudal fin. In the later larval stages of 17.0-24.4 mm a group of melanophores are added on medio-lateral part of the tail. These melanophores extend anteriorly and eventually cover the medio-lateral part of the whole body. In the early young stages of 97.2-34.8 mm the chromatophores cover the whole body surface in cloudy and H-shaped patterns. The chromatophore patterns of this stage are distinctive as generic characters of the fish. Fin membranes of the ventral fin appear in the post-larval stage (ca. 7.4 mm), and the primordial fin rays develop in the late post-larval stages (ca. 14.2 mm). The fin rays develop into a complete sucker in the young fish stage of around 30.0 mm in total length.

• Journal of the Korean Society of Food Science and Nutrition
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• v.8 no.1
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• pp.9-14
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• 1979

The consumption of catchup is increasing due to the raising of income level and food industry and westernization of dietary life followed by economic growth. In this paper I picked up three kinds of food produced by two foreign food companies and three kinds of food by two domestic companies. The average results by experimentation on the nutritive ingredients and the standard quality was as follows; A. Nutritve ingredients; 1) Water content a) Domestic 68.3% b) Foreign 69.1% 2) Protein content a) Domestic 2.2 g b) Foreign 2.1 g 3) Fat content a) Domestic 0.1 g b) Foreign 0.1 g 4) Carbohydrate content a) Domestic 25.1 g b) Foreign 24.6 g 5) Mineral content (Ash) a) Domestic 4.4 g b) Foreign 3.9 g 6) Calcium content a) Domestic 33.5 mg b) Foreign 24.2 mg 7) Phosphorus content a) Domestic 16.1 mg b) Foreign 24.2 mg 8) Vitamin C content a) Domestic 14.6mg b) Foreign 16.0 mg B. Standard quality 1) Remains after evaporation a) Domestic 41.7% b) Foreign 38.4% 2) Free mineral acid content a) Domestic none b) Foreign none 3) Tar chromatophore a) Domestic $trace(Acid)^+$ b) Foreign none 4) Heavy metalic $elements^*$ 5) Sodium chloride content a) Domestic 3.3% b) Foreign 3.3% 6) pH level a) Domestic 3.83 b) Foreign 3.76 + The tar chromatophore elements could not be accurately measured by chromatography. * The heavy metalic elements were both under safety levels in the domestic and foreign products.

• Animal cells and systems
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• v.5 no.4
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• pp.275-281
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• 2001

The zoea1 stages of Philyra kanekoi Sakai, 1934 were obtained by laboratory rearing. Three zoeal stages were described and illustrated in detail. Zoeal morphology was compared with those of other described species of the family Leucosiidae. Morphological comparisons with congeneric species revealed that zoeas of P. kanekoi were more similar to those of P. pisum and P. platychira than to other Philyra. The present material could be distinguished from other Philyrinae species by having no dorsal carapace spine, a spinous tip of the antenna, and no chromatophore on the base of a dorsal carapace spine. Within the family Leucosiidae, the zoeas of three Philyra (P. kanekoi, P. pisum and P. platychira) showed the greatest affinities with Leucosia sima and L. pubescens (Leucosiinae), and the number of setae on the basipod of the first maxilliped could be very useful characterisitcs for indentification between Philyra and Leucosia zoeas.

• Korean Journal of Environmental Biology
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• v.38 no.3
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• pp.416-423
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• 2020

The genus Petroderma never been reported from the coast of Korea. In this study, our collection from Chaeseokang is matched with P. maculiforme morphologically. Petroderma maculiforme is characterized by having small irregular light to dark brown crusts, a basal layer of irregularly shaped cells giving rise to erect parallel filaments which easily separate with pressure, single chromatophore per cell, small spherical to cylindrical unilocular sporangia in a terminal position, and plurilocular sporangia narrower than erect filaments or wider and shorter than erect filaments in a terminal position. In addition, Hineksia sordida was also collected from Korea. It is mostly epiphytic and characterized by uniseriate filamentous thalli forming loose tangled masses, sparse and spiral branching, some long lateral branches, rhizoids occurring throughout the plant, plurilocular and unilocular sporangia scattered on separate plants. Our molecular analyses based on the rbcL gene reveal that our samples of P. maculiforme and H. sordida are nested within the clades of Petroderma and Hincksia, respectively. Therefore P. maculiforme and H. sordida are reported as new records from Korea based on morphological and molecular analyses.

• The Korean Journal of Zoology
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• v.29 no.4
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• pp.261-271
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• 1986

The cutaneous xanthophore differentiation from larvae to adult in Bombina orientalis-the Korean fire bellied toad-is studied with light and electron microscopes. General structure of adult xanthophore which is composed of many pterinosomes and a small quantity of carotenoid vesicles is forming chromatophore complex with other dermal pigment cells. And the cytoplasmic process of xanthophore is distributed just beneath the basement membrane. The first differentiated xanthophore is originated from both rER rich cells and Golgi complex rich cells before and after the feeding larval stage. Formation of the pigment granule is proceeded gradually along the sequental metamorphic stages. After the metamorphosis, rapid multiplication of pterinosome is observed and enlargement of carotenoid vesicle is appeared after hybernation. These pigment granules are seen several structures by the differentiated level.

• BMB Reports
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• v.40 no.5
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• pp.697-707
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• 2007

Cytochrome $cbb_3$ oxidase is a member of the heme-copper oxidase superfamily that catalyses the reduction of molecular oxygen to the water and conserves the liberated energy in the form of a proton gradient. Comparison of the amino acid sequences of subunit I from different classes of heme-copper oxidases showed that transmembrane helix VIII and the loop between transmembrane helices IX and X contain five highly conserved polar residues; Ser333, Ser340, Thr350, Asn390 and Thr394. To determine the relationship between these conserved amino acids and the activity and assembly of the $cbb_3$ oxidase in Rhodobacter capsulatus, each of these five conserved amino acids was substituted for alanine by site-directed mutagenesis. The effects of these mutations on catalytic activity were determined using a NADI plate assay and by measurements of the rate of oxygen consumption. The consequence of these mutations for the structural integrity of the $cbb_3$ oxidase was determined by SDS-PAGE analysis of chromatophore membranes followed by TMBZ staining. The results indicate that the Asn390Ala mutation led to a complete loss of enzyme activity and that the Ser333Ala mutation decreased the activity significantly. The remaining mutants cause a partial loss of catalytic activity. All of the mutant enzymes, except Asn390Ala, were apparently correctly assembled and stable in the membrane of the R. capsulatus.