The objective of the present study was to determine the minimum alcohol (ethanol) concentration that gives rise to the coagulation of goat milk for the alcohol precipitation test, and to evaluate the physical parameters of goat milk which include alcohol and heat stability. A total of 1,295 udder-half milk samples from 648 lactating dairy goats were collected from seven farms in Jeonnam province, Republic of Korea, to determine the alcohol and heat stability. The majority (99.6%) of the samples were coagulated when 70% ethanol was added to the milk, while only 11.0% of the samples were precipitated by the addition of an equal volume of 45% ethanol. With the concentration of 65%, 60%, 55% and 50% aqueous ethanol, 99.2%, 96.8%, 81.0% and 52.8% of the milk samples were coagulated, respectively. Of 1,295 dairy goat milk samples tested for heat stability, 127 (9.8%) were coagulated by boiling. Among the 143 alcohol test-positive udder-half milk samples, 52 (4.0%) were unstable by heat test, while 1,032 (79.7%) of the 1,152 alcohol test-negative milk samples were stable by heat test. According to the results of boiling test, sensitivity and specificity of 45% alcohol precipitation test were 0.3023 (95% CI: 0.2346~0.3772) and 0.9190 (95% CI: 0.9017~0.9344), respectively. The contents of protein and the specific gravity were higher in the milk samples of 45% alcohol test-positive than in those of 45% alcohol test-negative. However, lower levels of lactose and milk urea nitrogen were observed in the milk samples of 45% alcohol test-positive compared to the alcohol test-negative milk samples. The lowest pH values ($6.73{\pm}0.20$) were shown in the 45% alcohol test-negative and heat-unstable milk samples, while the lowest values of somatic cell counts and bacterial counts were shown in the 45% alcohol test-negative and heat-stable milk samples. Results of this study suggest that the alcohol precipitation for dairy goat milk may have to be tested with ethanol concentration less than 45% for the determination of freshness and heat-stability.
We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae from crayfish in Jeollanam-do Province, Korea. The metacercariae were round, $180{\mu}m$ in average diameter, encysted with 2 layers of thick walls, but the stylet on the oral sucker was not clearly seen. The adult flukes were oval shape, and $760-820{\mu}m$ long and $320-450{\mu}m$ wide, with anterolateral location of 2 large testes. The phylogenetic tree based on ITS1 sequences of 6 M. spinulosus samples showed their distinguished position from other trematode species in GenBank. The most closely resembled group was Paragonimus spp. which also take crayfish or crabs as the second intermediate host. The present study is the first molecular characterization of M. spinulosus and provided a basis for further phylogenetic studies to compare with other trematode fauna in Korea.
The aim of this study is to identify the floor plan types of folk houses or traditional vernacular dwellings found in Nagan Folk Village located in Joellanamdo province. Examining the floor plans appeared among 36 vernacular folk houses presumably built in the 19th century in the village by means of the changes in the number of bays of Anche, the mail block of the house, the study was also able to construct a spatial compositional process of floor plan development. The floor plan examination revealed that the basic floor plan type in Nagan folk housing was '一' shape, a typical southern dwelling based on the existing classification. This basic type is consisted of three bays or rooms: Jeongji (kitchen), Anbang (large room), and Jageunbang (small room). New spaces or rooms are added to this three room house to expand the house as the residential functions become more complex, such as more living and storage spaces. The expansion appears to have two direction. On the one hand, it has been taken place by inserting Marea, an open wooden floor living space between Anbang and Jageunbang to meet the extended living demand. On the other, Jeongjibang, a second kitchen/storage has been attached to Jeongji outward for extra cooking and storage. This two-way expansion shows the trend of symmetric expansion between cooking, storing space and dwelling space. It can be implied that the arrangement of house rooms has been structurally formulated and shared by the farmer-builders in the 19th century in Nagan village who appeared to be influenced by fixed images for housing.
Purpose: The purpose of this study was to examine the rate of salivation before and after olfactory stimulation using BPO (black pepper oil) and then, to collect basic data for screening the possibility of introducing olfactory stimulus to prevent and improve dry mouth. Methods: The subjects in this study were 60 college students in Jeon-Nam province. The subjects were randomly divided into 3 groups; the BPO group (n=20), the lavender oil (LVO) group (n=20), and the distilled water (DW) group (n=20). The amount of whole saliva before and after olfactory stimulation was measured 14 times for a 4-week period. Results: In the BPO and LVO groups, the amount of whole saliva increased after the olfactory stimulation and it was most prominent in the BPO group (p<0.05). The difference of whole salivation was higher in the BPO group (p<0.05) and there was no significant difference in the salivary flow rate in the DW group. After 14 times of measurement for 4 weeks, the BPO and LVO groups showed an increase in salivary rate at an early stage compared without stimulation (p<0.05), but there was no significant difference between the 2 groups in the middle. Since the twelfth measurement, whole saliva secretion increased and maintained the elevated level (p<0.05). Conclusions: The olfactory stimulation using BPO may contribute to stimulating salivation. Further studies are needed for subjects with dry mouth symptoms. In the future, qualitative and quantitative studies should be conducted for people who complains about oral dryness.
This study was conducted to investigate the cause of a new duck disease occured in southern part of Korea. A meat type duck farm located in Kangjin, Chonnam Province had experienced outbreaks of septicemic disease at around 20 days of age in nearly every batch of ducklings from early spring to early summer in 1989. Main symptoms of the birds were eye and nasal discharge, depression, inappetence, diarrhea and nervous signs such as tremor and ataxia. Some birds died suddenly without any signs. Mortality reached from 20% to 80% in severe cases. The autopsy findings of the affected ducklings revealed consistantly severe airsacculitis, fibropericarditis, perihepatitis and occasionaly enteritis and distended ureter with urate deposit. A rod shaped gram-negative bacterium was isolated purely from brain and liver of the diseased ducks by culturing the specimens on blood agar for 48 hours in candle jar. The isolate neither produced hemolysis nor grew on MacConKey Agar. It formed colony relatively slowly being recognizable at least 36 hours after culturing, reaching colony size of about 1mm in diameter at 48 hours culture. The colony looked iridescent under oblique light and had muddy odor. The isolate did not ferment carbohydrates tested but produced gelatinase, hippuricase and oxidase which were considered as characteristics of P anatipestifer. The isolate induced similar signs and lesions when infected experimentally into ducks of 3 to 38 days age via intraperitoneum or intratrachea. However it did not produce any clinical signs wen inoculated via intranasal route. It produced only mild signs in chicken just injected with a very large dose. The bacteria did not produce any signas or lesions in mice. It was concluded through biochemical and physiological tests and animal inoculation tests that the new disease was caused by P anatipestifer.
In order to revitalize rural areas fundamentally through multifunctional utilization of their resources, it should be necessary to prepare the rational development plan to the areal characteristics and conditions, and the first priority of its planning works should be given to spatial planning. The space syntax method, a powerful objective and quantitative analysis tool on the relationship between social and spatial characteristics, was introduced in this study. Five Comprehensive Rural Clustered Villages Development Areas in the Jeonnam-province were selected as case study areas, of which total area's and included villages' spatial variables were measured and analyzed. Rural villages analyzed in this study have the spatial structure badly systematized and much complicated, which results from low integration and deep spatial depth of them. And, by virtue of relatively many axial lines, there should be few differences between villages in terms of local integration, connectivity and control, while being significant difference in terms of global integration showing the whole areal characteristics. Intelligibility, the correlation coefficient between connectivity(local variable) and integration(global one) is low, which means that the spatial structure of the study areas is difficult for visitors to understand the area or village well. Spatial configuration analysis results in the case study areas showed that each development area has a unique spatial structure and is differentiated in terms of not only local spatial variables but also global spatial variables. Therefore, global and local characteristics should be considered in spatial analysis of development areas.
Wang, Ah Rha;Kim, Min Jee;Kim, Sung Soo;Kim, Iksoo
International Journal of Industrial Entomology and Biomaterials
/
v.35
no.1
/
pp.51-57
/
2017
The tiny dragonfly, Nannophya pygmaea (Odonata: Libellulidae), is an endangered insect in South Korea. Previously, a partial mitochondrial DNA sequence that corresponded to a DNA barcoding region has been used to infer genetic diversity and gene flow. In this study, we additionally sequenced the barcoding region from N. pygmaea that had been collected from three previously sampled populations (40 individuals) and these sequences were combined with the preexisting data. We also selected and sequenced an additional mitochondrial gene (ND5) to find further variable gene regions in the mitochondrial genome. DNA barcoding sequences of 108 individuals from five South Korean localities showed that genetic diversity was highest in Gangjin, Jeollanam-do Province. Muuido, which was previously occupied by a single haplotype, was also found to have an identical haplotype, which confirmed the low genetic diversity on this islet. Gene flow among populations is highly limited, and no clear distance- or region-based geographic partitioning was observed. Phylogenetic relationships among haplotypes showed that there were no discernable haplotypes in South Korea. ND5 provided slightly more haplotypes compared to the barcoding region in 40 individuals (14 vs. 10 haplotypes in the COI gene). It also had a slightly higher within-locality diversity estimate, which suggested that ND5 had potential as mitochondrial DNA-based marker for population genetic analysis.
Journal of Family Resource Management and Policy Review
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v.13
no.4
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pp.141-169
/
2009
This study investigated the effect of the money resource on the resource use behaviors of undergraduate students. The aim of the study was the examination of the relationship between undergraduate income and clothes-purchasing behavior. A total of 415 undergraduates residing in Gwangju City and Chonnam Province were interviewed by means of a questionnaire. SPSS 12.0 software was used for statistical analysis. Cronbach's $\alpha$, t-test, one-way ANOVA, Duncan's multiple range analysis and chi-square analysis were all conducted to acquire the results. The main results of this study are as follows. First, the average undergraduate income average was relatively high. Second, the motive for clothing purchases by undergraduates was to buy clothes similar to those which they already had. The undergraduates acquired their purchasing information from past experiences of buying clothes and considered design, quality and color when choosing their clothing. They purchased their clothes at retail stores and on average bought clothes about once a month. Although theaverage monthly expenditure on clothes was over 100,000 won, in general, the undergraduates spent between 50,000 and 100,000 won a month. They mainly paid cash when they purchased their clothes. Third, by analyzing the income, clothes-purchasing behavior, and clothes purchasing frequency of undergraduates, significant statistical differences in average monthly clothes expenditure and payment methods were detected. The results of the study can be used to understand the patterns of undergraduate money resource usage behaviors and can be utilized as an educational resource at home and within educational institutions.
Purpose: This study was conducted to develop Web-based multimedia contents for supporting student nurses' clinical practice on critical care, and to evaluate learners' responses. Methods: Based on the steps of Assessment, Design, Development, Implementation, & Evaluation(ADDIE) model, a total of 13 self-directed learning modules including live lectures and real video clips were developed through faculty collaboration of nine nursing colleges in Gwangju and Chonnam province. The finally developed multimedia contents were published on the Web of the learning management system at a local e-learning center. Results: The Web contents were evaluated after self-learning by 81 junior college nursing students who were encouraged to study it at their own pace during their two-week clinical practice at a medical or surgical intensive care unit of a university hospital and two hospitals. The knowledge (t = -27.66, p < .001) and self-evaluated clinical performance level(t = 7.54, p < .001) were significantly increased after learning of the Web contents and clinical practice, and satisfaction level that measured post-test only was 4.0 out of 5 point. Conclusion: The use of Web contents for critical care need to be extended as a complimentary material in a class room lecture or clinical practice of students to increase their self-learning ability and understandings of clinical knowledge and situation.
Journal of Korean Library and Information Science Society
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v.43
no.3
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pp.407-432
/
2012
This study is carried out to investigate the pubishing culture in Chonnam province during the latter half of Chosun Dynasty. The subjects, dates, and places of publication are analyzed from the books which have been found in the publication records and the catalog of woodblocks. Right after the war, Samryakjikhae and Sohakjipsul were published at Jukchunseodang, Bosung area. Anthologies were published dominantly through the whole period. Naju was the center of publication in 17 & the first half term 18 centuries and then moved to Jangwheung area. In this period, publication of genealogical records, biographies, anthologies, geography books had been increasing vastly. From the 19 century, most books were published by using the wooden type. The wooden type was used because the contents of books were for the limited number of publication. The most active publication happened at the end of 19th century. Significance of Confucian literature, collective anthologies, medical science, and confucious classics decreased in the latter half.
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