• Title/Summary/Keyword: Chlorophyta

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Morphological, Molecular, and Biochemical Characterization of Astaxanthin-Producing Green Microalga Haematococcus sp. KORDI03 (Haematococcaceae, Chlorophyta) Isolated from Korea

  • Kim, Ji Hyung;Affan, Abu;Jang, Jiyi;Kang, Mee-Hye;Ko, Ah-Ra;Jeon, Seon-Mi;Oh, Chulhong;Heo, Soo-Jin;Lee, Youn-Ho;Ju, Se-Jong;Kang, Do-Hyung
    • Journal of Microbiology and Biotechnology
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    • v.25 no.2
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    • pp.238-246
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    • 2015
  • A unicellular red microalga was isolated from environmental freshwater in Korea, and its morphological, molecular, and biochemical properties were characterized. Morphological analysis revealed that the isolate was a unicellular biflagellated green microalga that formed a non-motile, thick-walled palmelloid or red aplanospore. To determine the taxonomical position of the isolate, its 18S rRNA and rbcL genes were sequenced and phylogenetic analysis was performed. We found that the isolate was clustered together with other related Haematococcus strains showing differences in the rbcL gene. Therefore, the isolated microalga was classified into the genus Haematococcus, and finally designated Haematococcus sp. KORDI03. The microalga could be cultivated in various culture media under a broad range of pH and temperature conditions. Compositions of the microalgal cellular components were analyzed, and its protein, carbohydrate, and lipid compositions were estimated to be 21.1 ± 0.2%, 48.8 ± 1.8%, and 22.2 ± 0.9%, respectively. In addition, D-glucose and D-mannose were the dominant monosaccharides in the isolate, and its amino acids were composed mainly of aspartic acid, glutamic acid, alanine, and leucine. Moreover, several polyunsaturated fatty acids accounted for about 80% of the total fatty acids in Haematococcus sp. KORDI03, and the astaxanthin content in the red aplanospores was estimated to be 1.8% of the dry cell weight. To the best of our knowledge, this is the first report of an Haematococcus sp. isolated from Korea, which may be used for bioresource production in the microalgal industry.

Optimum Culture Environment of the Benthic Copepod, Tigriopus japonicus (부착성요각류 Tigriopus japonicus의 최적배양환경)

  • PARK Huem Gi;HUR Sung Bum
    • Journal of Aquaculture
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    • v.6 no.3
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    • pp.147-157
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    • 1993
  • The harpacticoid copepod, Tigriopus japonicus is one of the most important zooplankton as a live food for the production of marine fish fry. Thus, the salinity tolerance and the optimum culture environment of this copepod in terms of salinity, temperature and light were examined. The food values of 6 kinds of phytoplankters and 2 kinds of yeast were also investigated for mass culture of this copepod. The results are as follows: After 5 day culture in the experiment of salinity tolerance, the survival rates of the gravid female at $0\%\;and\;90%o\;were\;40\%\;and\;70\%$, respectively. However, at salinity ranging from $2\%o\;to\;80\%o$, high survival rates above $85\%$ were observed. It means T. japonicus is very euryhalinous species. Temperature was more important factor than salinity for the fecundity of T. japonicus. The optimum culture conditions of this species were $24^{\circ}C,\;24\%o$, and 3,000 lux with 24 L: 0D. Under these culture conditions, the average fecundity from a gravid female per spawning was 38 nauplii, and the interval time between spawnings were 2.05 days. Phaeodactylum tircornutum seemed to be the most suitable phytoplankton as a live food for T. japonicus, and the large chlorophyta, Tetraselmis suecica showed the lowest food value among 6 phytoplankters and 2 yeasts. The food value of w-yeast was better than that of baker's yeast, and it is similar to that of phytoplankton such as Amphora sp., Chlorella ellipsoidea and Nannochloris oculata. So, the w-yeast seems to be appropriate food source for mass culture of T. japonicus.

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Salinity affects microbial community structure in saemangeum reclaimed land

  • Kim, Kiyoon;Samaddar, Sandipan;Ahmed, Shamim;Roy, Choudhury Aritra;Sa, Tongmin
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.364-364
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    • 2017
  • Saemangeum reclaimed land is a part of Saemangeum Development Project. Most of the persistent problems of Saemangeum reclaimed land remain to be related to soil salinity. Soil salinity is a major abiotic factor related to microbial community structure and also fungi have been reported to be more sensitive to salinity stress than bacteria. The aim of this study was conducted to investigate the effect of soil salinity levels on the microbial communities in Saemangeum reclaimed land using 454 pyrosequencing analysis. Soil samples was collected from 12 sites of in Saemangeum reclaimed land. For pyrosequencing, 27F/518R (bacteria) and ITS3/ITS4 (fungi) primers were used containing the Roche 454 pyrosequencing adaptor-key-linker (underlined) and unique barcodes (X). Pyrosequencing was performed by Chun's Lab (Seoul, Korea) using the standard shotgun sequencing reagents and a 454 GS FLX Titanium sequencing System (Roche, Inc.). In the soil samples, Proteobacteria (bacteria) and Ascomycota (fungi) shows the highest relative abundance in all the soil sample sites. Proteobacteria, Bacteroidetes, Plantomycetes, Gemmatimonadetes and Parcubacteria were shown to have significantly higher abundance in high salinity level soils than low salinity level soils, while Acidobacteria and Nitrospirae has significantly higher relative abundance in low salinity level soils. The abundance of fungal, Ascomycota has the highest relative abundance in soil samples, followed by Basidiomycota, Chlorophyta, Zygomycota and Chytridiomycota. Basidiomycota, Zygomycota, Glomeromycota and Cerozoa were show significantly higher relative abundance in low salinity level soils. The principal coordinate analysis (PCoA) and correlation analysis shown to salinity-related soil parameters such as ECe, Na+, SAR and EPS were affected to bacterial and fungal community structure. Proteobacteria, Bacteroidetes, Plantomycetes exhibited significantly positive correlation with soil salinity, while Acidobacteria exhibited significantly negative correlation. In the case of fungal community, Basidiomycota and Zygomycota were seen show significantly negative correlation with salinity related soil parameters. These results suggest that provide understanding effect of soil salinity on microbial community structure and correlation of microbial community with soil parameters in Saemangeum reclaimed land.

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AN OBSERVATION Of WATER QUALITY AND RED WATER IN STILL WATER FISH PONDS (정수양어지에서의 수질관찰과 적조현상(물변화)에 관한 보고)

  • KIM In-Bae;PARK Myeong Ja
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.7 no.2
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    • pp.69-78
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    • 1974
  • A study on the contents of dissolved oxygen and phytoplankton in the pond water has been carried out to determine the water quality and to investigate the cause of red water in fish ponds at Pusan Fisheries College in 1972-1973. The results obtained are as follows : 1. Measured oxygen content during the period from 6th to 7th of August 1972 was: 23.95ppm ($328\%$ in saturation rate) in daytime and 0.39 ppm($5\%$) in the early morning in the water which contains heavy bloom of phytoplankton, on the other hand, in clear water the range of oxygen content was 8.72 to 6.58ppm. 2. The occurrences of red water in 1972-1973 were always caused by the sudden death of Synechocystis aquatilis (Cyanophyta) when the water temperature was very high ($30-33.5^{\circ}C$) and then high mortality of fish under rearing followed. 3. The phytoplankon observed during this study period was 133 species belonging to 55 genera in 23 families, and those of Chlorophyta was dominant in number of species, and Cyanophyta in quantity. 4. Zooplankton observed was 12 species belonging to 11 genera in 11 families, and they were very small both in number of species and in quantity compared to those of phytoplankton.

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Evaluation of In-vitro Anticoagulation Activity of 35 Different Seaweed Extracts (35종 해조류 추출물의 in-vitro 항혈전 활성 평가)

  • Ahn, Seon-Mi;Hong, Yong-Ki;Kwon, Gi-Seok;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.20 no.11
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    • pp.1640-1647
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    • 2010
  • Seaweeds have been recognized as a health food, having anti-obesity, anti-constipation and anticoagulation activities, and the use of seaweeds in the food, medicine, and cosmetic industries have recently significantly increased. In this study, methanol extracts were prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta), and thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (aPTT) were determined in order to develop safe and novel anticoagulation agents from natural products. In TT experiments, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis (Kjellman) Setchell, Ishige foliacea, I. okamurai, Sargassum confusum and S. yamade showed strong thrombin inhibition activity among the 35 different seaweeds. In PT experiments, the inhibitions of prothrombin were identified in the selected seaweeds from TT experiment, with the exception of S. yamade. In aPTT experiments, the seaweeds with blood coagulation inhibition factors were E. cava, E. stolonifera, E. bicyclis (Kjellman) Setchell, I. foliacea, I. okamurai, S. confusum and Hixikia fusiforme Okamura. Further anticoagulation assay with the selected 8 seaweeds suggested that S. confusum is most effective in antithrombosis, and E. stolonifera, E. bicyclis (Kjellman) Setchell, and I. foliacea have high potential as antithrombosis agents. Based on components-activity correlation analysis, flavonoids are considered as active anticoagulation components of seaweeds These results suggest that edible seaweeds, especially S. confusum, have potential as safe and novel anticoagulants, and S. yamade and H. fusiforme Okamura could be used as a thrombin-specific and coagulation factor-specific inhibitors.

Evaluation of Antioxidant and Nitrite Scavenging Activity of Seaweed Extracts (해조류 추출물의 항산화 및 nitrite 소거활성 평가)

  • Ahn, Seon-Mi;Hong, Yong-Ki;Kwon, Gi-Seok;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.21 no.4
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    • pp.576-583
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    • 2011
  • In the course of study for the development of functional food ingredients from seaweeds having useful biological activities, the in-vitro antioxidant and nitrite scavenging activities of the methanol extracts prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta) were determined. At $500\;{\mu}g/ml$ concentration of the methanol extracts, Ecklonia cava, Ecklonia stolonifera, Eisenia bicyclis (Kjellman) Setchell, Ishige foliacea, I. okamurai, Sargassum confusum, S. fulvellum, S. yamade and Zostera marina showed 60% more DPPH and ABTS scavenging activity. The exceptions were found in I. okamurai and Z. marina, which showed 45% and 53% ABTS scavenging activity, respectively. The correlation coefficient between DPPH and ABTS scavenging activities was 0.855, suggesting that the 9 different seaweeds extracts could scavenge anion and cation radicals concurrently. In reducing power, only E.cava, E.stolonifera and E.bicyclis showed above 0.88 ($Abs_{700}$). In a while, in nitrite scavenging activity at $100\;{\mu}g/ml$ concentration of the methanol extracts, 19 different seaweeds extracts including I. foliacea, I. okamurai, S. confusum, S. fulvellum, and S. yamade showed 60% more activity. Calculation of $IC_{50}s$ of DPPH, ABTS and nitrite scavenging activities of 9 different seaweeds extracts further showed that I. foliacea and S. yamade, besides E.cava, E.stolonifera and E.bicyclis, have strong antioxidant and nitrite scavenging activity. These results suggest that the selected 9 different seaweeds could be developed as functional food ingredients and I. foliacea and S. yamade have potential as novel natural sources of antioxidant and nitrite scavenger.

Classification of the Edible Plants on the Market in Busan (시장에 유통되는 식용식물의 분류)

  • 문성기;정순해;최철만
    • Journal of Life Science
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    • v.13 no.6
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    • pp.764-774
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    • 2003
  • The edible plants sold at the market in Busan were classified into 8 divisions, 11 classes, 46 orders, 67 families, 156 genera, 183 species and 45 varieties, and a total of 228 kinds of plants were included in them. Among the 228 kinds of edible plants, Angiospermae were the highest number of 202 kinds (88.60%), and next came Fungi of 9 kinds (3.95%), Phaeophyta of 5 kinds (2.19%), Rhodophyta of 4 kinds (1.75%), Pteridophyta of 3 kinds (1.32%) Gymnospermae of 2 kinds (0.88%), Chlorophyta of 2 kinds (0.88%), and Lichenes of 1 kind (0.44%) in order In the taxon of family, Cruciferae, Rosaceae, Leguminoceae, Cucurbitaceae, Umbelliferae, Compositae, Liliaceae, and Gramineae include many species. In the taxon of genus, Brassica(14 species), Prunus(9), Lactuca(9), Allium(7), Citrus(5), Cucumis(4), Cichorium(4) in order contain many species. Among tile edible plants, Chamjuknamu(Cedrela sinensis) is wrongly named Gajuknamu(Ailanthns altissima). And there are others which are called in a wrong way: Endive is wrongly called Chicory, Saeneutaribeoseot, Saesongi and Padeudeknamul(Japanese hornwort), Chamnamul. Gyul and Milgam, Geumgam and Gamgyul, Banana and Pacho, Paprika and Pimang(Pimento) are the names of the same plants. The number of the kinds of edible plants is different in each season. Fall has the most edible plants, and it is followed by spring, summer and winter. The edible parts of plants which are sold at the market are leaves, young sprouts, fruits, grains, roots, stems and petioles.

Studies on the Floristic Composition and Periodicity at Joomoonjin Sea Bank (주문진(主文津) 인공(人工) 방파제(防波堤)에 서식(棲息)하는 해조상(海藻相) 및 주기성(週期性)에 관한 연구(硏究))

  • Kim, Yang-Bae
    • Journal of Fisheries and Marine Sciences Education
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    • v.3 no.2
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    • pp.29-46
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    • 1991
  • Benthic marine algal community of a sea bank at Joomoonjin, in the eastern coast of Korea, was investigated monthly in view of floristic composition and seasonal periodicity during from August, 1989 to July 1990. Total number of benthic alga identified were 70 taxa, which composed of 7 species of Chlorophyta, 21 species of Phaeophyta and 42 species of Rhodophyta. The dominant species were Sargassum horneri, Underia pinnatifida, Laminaria japonica and Pachymeniopsis sp.. Vegetation, from the point of phygiognomy, was a mosaic type by these dominant species. The flora was compared with similarity index using S${\phi}$rensen coefficient and grouped by a cluster analysis. As a result, the algal community of this area could be divided into two clusters at similarity level 45% : Flora of water temperature-ascending period(January-July) and flora of water temperature-descending period(August-December). And also, the former flora was subdivided into two clusters at point of the $14^{\circ}C$ sea water temperature : winter flora(January-April) and spring-summer flora(May-July). The flora of this area was characterized as a mixed flora of cold and temperature zone by Cheney's index.

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Limno-Biological Investigation of Lake Ok-Jeong (옥정호의 육수생물학적 연구)

  • SONG Hyung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.15 no.1
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    • pp.1-25
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    • 1982
  • Limnological study on the physico-chemical properties and biological characteristics of the Lake Ok-Jeong was made from May 1980 to August 1981. For the planktonic organisms in the lake, species composition, seasonal change and diurnal vertical distribution based on the monthly plankton samples were investigated in conjunction with the physico-chemical properties of the body of water in the lake. Analysis of temperature revealed that there were three distinctive periods in terms of vertical mixing of the water column. During the winter season (November-March) the vertical column was completely mixed, and no temperature gradient was observed. In February temperature of the whole column from the surface to the bottom was $3.5^{\circ}C$, which was the minimum value. With seasonal warming in spring, surface water forms thermoclines at the depth of 0-10 m from April to June. In summer (July-October) the surface mixing layer was deepened to form a strong thermocline at the depth of 15-25 m. At this time surface water reached up to $28.2^{\circ}C$ in August, accompanied by a significant increase in the temperature of bottom layer. Maximum bottom temperature was $r5^{\circ}C$ which occurred in September, thus showing that this lake keeps a significant turbulence Aehgh the hypolimnial layer. As autumn cooling proceeded summer stratification was destroyed from the end of October resulting in vertical mixing. In surface layer seasonal changes of pH were within the range from 6.8 in January to 9.0 in guutuost. Thighest value observed in August was mainly due to the photosynthetic activity of the phytoplankton. In the surface layer DO was always saturated throughout the year. Particularly in winter (January-April) the surface water was oversaturated (Max. 15.2 ppm in March). Vertical variation of DO was not remarkable, and bottom water was fairly well oxygenated. Transparency was closely related to the phytoplankton bloom. The highest value (4.6 m) was recorded in February when the primary production was low. During summer transparency decreased hand the lowest value (0.9 m) was recorded in August. It is mainly due to the dense blooming of gnabaena spiroides var. crassa in the surface layer. A. The amount of inorganic matters (Ca, Mg, Fe) reveals that Lake Ok-Jeong is classified as a soft-water lake. The amount of Cl, $NO_3-N$ and COD in 1981 was slightly higher than those in 1980. Heavy metals (Zn, Cu, Pb, Cd and Hg) were not detectable throughout the study period. During the study period 107 species of planktonic organisms representing 72 genera were identified. They include 12 species of Cyanophyta, 19 species of Bacillariophyta, 23 species of Chlorophyta, 14 species of Protozoa, 29 species of Rotifera, 4 species of Cladocera and 6 species of Copepoda. Bimodal blooming of phytoplankton was observed. A large blooming ($1,504\times10^3\;cells/l$ in October) was observed from July to October; a small blooming was present ($236\times10^3\;cells/l$ in February) from January to April. The dominant phytoplankton species include Melosira granulata, Anabaena spiroides, Asterionella gracillima and Microcystis aeruginota, which were classified into three seasonal groups : summer group, winter group and the whole year group. The sumner group includes Melosira granulate and Anabaena spiroides ; the winter group includes Asterionella gracillima and Synedra acus, S. ulna: the whole year group includes Microtystis aeruginosa and Ankistrodesmus falcatus. It is noted that M. granulate tends to aggregate in the bottom layer from January to August. The dominant zooplankters were Thermocpclops taihokuensis, Difflugia corona, Bosmina longirostris, Bosminopsis deitersi, Keratelle quadrata and Asplanchna priodonta. A single peak of zooplankton growth was observed and maximum zooplankton occurrence was present in July. Diurnal vertical migration was revealed by Microcystis aeruginosa, M. incerta, Anabaena spiroides, Melosira granulata, and Bosmina longirostris. Of these, M. granulata descends to the bottom and forms aggregation after sunset. B. longirostris shows fairly typical nocturnal migration. They ascends to the surface after sunset and disperse in the whole water column during night. Foully one species of fish representing 31 genera were collected. Of these 13 species including Pseudoperilnmpus uyekii and Coreoleuciscus splendidus were indigenous species of Korean inland waters. The indicator species of water quality determination include Microcystis aeruginosa, Melosira granulata, Asterionelta gracillima, Brachionus calyciflorus, Filinia longiseta, Conochiloides natans, Asplanchna priodonta, Difflugia corona, Eudorina elegans, Ceratium hirundinella, Bosmina longirostris, Bosminopsis deitersi, Heliodiaptomus kikuchii and Thermocyclops taihokuensis. These species have been known the indicator groups which are commonly found in the eutrophic lakes. Based on these planktonic indicators Lake Ok-Jeong can be classified into an eutrophic lake.

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Differentiation of Some Environmental Factors and Planktonic Communities of the Two Areas Divided by the Breakwater Between Youngdo and Jodo, Busan (부산시 영도와 조도사이의 방파제 양측 해역의 환경요소 및 부유생물상의 차이에 관하여)

  • MIN Byoung Seo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.4
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    • pp.243-258
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    • 1977
  • 1 The coastal area between Youngdo and Jodo was a common coastal water not much different from other coastal waters before the construction of the breakwater between them. 2. The breakwater between the two islands shuts off the tidal currents and divides the area . into the two small isolated bays to create quite different environments. 3. To understand the differences between then, present study examined some environmental factors such as water temperature, salinity, dissolved oxygen, transparency, and major nutrients, phosphates, sillicates and nitrites and the phytoand zooplankton. The samplings were carried out monthly from March 1976 to February 1977 at 4 stations: 2 stations in each bay. 4. Some differences were observed in the environmental factors such as water temperature, salinity, dissolved oxygen and transparency between the two bays. 5. The distribution and occurence of nutrient salts of the two bays were distinctly different each other. Northern Bay had $138\%$ of nutrients in comparison with Southern Bay. 6. Phytoplankton in Northern Bay was about $200\%$ plentier than in Southern Bay. 7. Zooplankton in Southern Bay was about $180\%$ richer than in Northern Bay. 8. One of the pollution indicator species, Synedra ulna, was observed in Northern Bay and the occurence of Euglena sp. and ciliates were much higher in Northern Bay than in Southern Bay, but, in contrast, Sagitta sp. was more abundant in Southern Bay than in the other. 9. The areas of the two bays seem to be in its way to eutrophication especially in Northern Bay. 10. The two bays have been differentiated enough to identify each other.

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