• Journal of Bio-Environment Control
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• v.14 no.1
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• pp.56-62
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• 2005

This study was conducted to investigate the effect of grafting methods on plug seedling quality, growth after transplanting, and yield of oriental melon that those seedlings were raised in the 32 hole plug tray for protected cultivation. Number of leaf and leaf area in approach grafting were higher until 15 days after grafting but lower on 20 days after grafting. Plant height, stem diameter and content of chlorophyll were nearly the same of each grafting methods on 20 days after grafting. Growth of top part, that is, plant height, leaf number, and leaf area on root removed single cotyledon ordinary splice grafting was the highest among the grafting methods. In the underground part, dry weight of root was high and T/R ratio was the lowest. Seedling quality of root removed single cotyledon ordinary splice grafting was the highest among grafting methods before transplanting. Plant height, number of leaf, leaf area and dry weight of root removed single cotyledon ordinary splice grafting in the growth of field were the highest on 30 days after grafting among grafting methods and days required for first flowering was also shorter, 38.4 days among grafting methods. But Fruit weight, content of soluble solids, fruit hardness, and color characteristics in fruit quality were insignificant among each grafting methods. Consequently, grafting methods influenced on the seedling quality, of oriental melon early growth after transplanting and yields. It was concluded that root removed single cotyledon ordinary splice grafting was the best methods in present study. It will be needed to convert grafting methods. Thus new grafting method should be applied.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.240-244
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• 2003

The purpose of this study was to investigate a proper $Ca^{2+}\;:\;K^+$ ratio in nutrient solution to produce marjoram and oregano by hydroponics. Two herbs were grown for 40 days with the $Ca^{2+}\;:\;K^+$ ratio of 3.5;13, 4.5:11(standard herb's nutrient solution), 5.5:9 and 6.5:7mM${\cdot}L^{-1}$ of herb's nutrient solution developed by European Vegetable R &D Center, Belgium. The ratio of 5.5:9, and 6.5:7 mM${\cdot}L^{-1}$ showed better results in growth of marjoram than other treatments. Vitamin C contents and amounts of esential oil were the highest in the ratio of 5.5:9 and 6.5:7, respectively. Inoregano, the growth and vitamin C contents were the highest in the ratio of 5.5:9 and 6.5:7 mM${\cdot}L^{-1}$, respectively. The content (%) and amount (mg/plant) of essential oil were the highest in the ratio of 6.5:7mM${\cdot}L^{-1}$. These results indicated that 5.5:9 and 6.5:7 mM${\cdot}L^{-1}$were proper $Ca^{2+}\;:\;K^+$ ratio of nutrient solution of marjoram and oregano, respectively, by hydropoinics.

• Journal of Plant Biology
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• v.35 no.2
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• pp.117-123
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• 1992

The effect of benzyladenine (BA) on lipid peroxidation and compositions of total insoluble proteins and chloroplast thylakoid protein from wheat primary leaves during senescence in the dark was studied. BA ($10^{-5}\;M$) treatment prevented conspicuously the loss of chlorophyll content and soluble and insoluble leaf protein contents in senescing wheat leaf segments during 4-day dark incubation. Under the BA treatment, especially, the level of insoluble protein was highly maintained than that of soluble protein. Also, the increase of malondialdehyde (MDA: the peroxidation product of membrane lipids) content was inhibited in the BA treated leaves. Three major polypeptide bands in quantity corresponding to 57, 26 and 12 KD molecular weight were clearly resolved with other minor bands by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in the insoluble protein fraction. The insoluble protein profiles of the control leaves showed a remarkable decrease in the intensity of the 57 and 12 KD band except for 26 KD band in the 72 h dark incubation. This loss during dark incubation was reduced by BA treatment. More than 20 polypeptides were resolved in the chloroplast thylakoid membrane fraction with the most prominent bands which are 59 and 57 KD ($\alpha\;and\;\beta$ subunit of coupling factor: CF) and 26 KD (apoprotein of LHCP). The changes in thylakoid protein profile during 72 h dark incubation showed the rapid degradation in control, but this degradation was prevented in quantity by BA treatment. The above results suggested that BA would inhibit the peroxidation of membrane lipids, thereby preventing the loss of membrane proteins which led to the maintenance of the membrane integrity including chloroplast thylakoid.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.5
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• pp.391-397
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• 1993

Rye(Secale cereale cv. Chunchu), barley(Hordeum vulgare cv. Dusan 29#), and italian ryegrass(Lolium multiflorum cv. Barmultra) were treated at 0, 0.1, 0.3, and 0.6 % of NaCl solution for 30 days. Plant height, leaf length, and leaf area were decreased with increasing NaCl level, and leaf area was decreased mainly due to decrease of leaf length. Root length was decreased at higher NaCl levels, but root number was increased. Shoot dry weight was decreased by increasing NaCl levels in rye and italian ryegrass, but barley did not show significant change. Dry weight of root was more decreased than that of shoot at higher NaCl levels. Chlorophyll content was decreased, but electric contuctivty of shoots and roots was increased at higher NaCl levels. Na+ content in shoot and root was increased with increasing NaCl level but the degree was not same. Reducing power of roots was that three crops as affected by NaCl was not equal. Barley was the most tolerant to NaCl stress.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.420-427
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• 1995

This study was carried out to investigate heterosis in photosynthetic characteristics and yield of F$_1$ hybrid rice using genetic cytoplasmic male sterile lines. Two F$_1$ hybrid rices, HR7028A/Yongmoonbyeo and IR54756A/Yongmoonbyeo, showed heterobeltiosis in dry weight and leaf area at the heading date and at the 20 day after heading. HR7028A/Yongmoonbyeo showed heterobeltiosis and IR54756A/Yongmoonbyeo showed heterosis in chlorophyll content and RuDPCase activity in flag leaf, T-sugar and starch content in stem, and $CO_2$ fixation at the 20 day after heading as well as at the heading date. HR7028A/Yongmoonbyeo showed heterobeltiosis in number of panicles and grains per panicle, and IR54756A/Yongmoonbyeo had heterobeltiosis in number of panicles per plant. As results, heterobeltiosis of HR7028A/Yongmoonbyeo in yield was 11% and that of IR54756A/Yongmoonbyeo was 4%.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.201-201
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• 2017

Delayed stem senescence of soybean is a phenomenon of retarded leaf and stem yellowing, where plants maintain a high stem water content and remain chlorophyll in leaf and stem at maturity stage. This phenomenon was one of the most important physiological disease in Japanese soybean cultivation. The occurrence of delayed stem senescence was affected by sowing time. And the most of Japanese field, soybean seeds were sowed in June. June is the rainy season in Japan, and the soil water content of field become higher in this season. In this study, the effects of late sowing (July sowing) on the yield and the occurrence of delayed stem senescence in soybean cultivars Enrei, Tachinagaha and Ayakogane were examined from 2013 to 2015, in the experimental farm at Nihon University (Fujisawa-city, Kanagawa, Japan). The seeds of all cultivars were sowed in June (June-normal density plot) or July (July-normal density plot, July-high density plot and July-super high density plot) in field experiment. The pot experiments were carried out in 2014. In all cultivars, the yield of July-high density plot and July-super high density were higher than that of June normal density plot. And the yield of June-normal density plot was the same as that of July-normal density plot. In all cultivars, the occurrence of delayed stem senescence was increased by seeding in June sowing. And in July sowing plots, no significance difference in the occurrence of delayed stem senescence was observed among density plots. One of reason about the increasing the occurrence of delayed stem senescence in June-normal plot was the increasing of the damaged seeds by bean bugs. Add one of reason about the decreasing of the occurrence of delayed stem senescence of July plots was the decreasing of the amount of cytokinin supplied from root to top and water stress after the flowering time was improved compared with the June plot. In conclusion, the yield of Enrei, Tachinagaha and Ayakogane were not changed by changing the sowing time from June to July. In all cultivars, the occurrence of delayed stem senescence were decreasing by seeding in July.

• Korean Journal of Plant Tissue Culture
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• v.27 no.6
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• pp.453-459
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• 2000

Three rice (Oryza sativa L. var Dong-Jin) mutants (DTR1, DTR2, DTR3) resistant to S-methyltryptophan (5MT) were selected by mutagenized M3 seeds. The frequency of chlorophyll mutations induced by the EMS (0.2%) treatment performed 2 hours after flowering is clearly higher than that induced by other treatments in M1 generation. Progeny obtained from the self-pollinating of 5MT-resistant lines segregated with 3 : 1 of resistant to sensitive ratio. Furthermore, the ratio of homozygote to heterozygote in 5MT-resistant plants of the M4 generation was 1：2. These results show that 5MT resistance was inherited as a single dominant nuclear gene. The resistance was also expressed in callus derived from seeds. Total free amino acid content in homozygous seeds of DTR1 and DTR2 showed about 1.7 fold-increased compared to the wild-type seeds. In particular, the levels of phenylalanine and Iysine were, respectively, 6.2 and 3.2 times higher than those in the wild-type seeds. However, seeds of DTR3 had lower levels of free amino acid than the wild-type seeds. This result indicate that these mutants as a significant step towards the production of new rice with balanced amino acid content.

• Korean Journal of Environmental Agriculture
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• v.15 no.3
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• pp.341-347
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• 1996

A green-house experiment was conducted to investigate the growth of Chinese cabbage, radish, and soybean and changes in soil chemical properties after application of 5 and 10mm of pH 2.7 simulated acid rain(SAR) followed by 0, 5, and 10mm of pH 6.0 normal water at the three-day intervals 20 times for Chinese cabbage and radish and 42 times for soybean. The results obtained are summarized as follows: 1. Visual damages by SAR were white-yellow leaf spots, dark brown or light green leaf color, and wrinkled leaf margins in all crops. The degree of visual damages was severer at 10mm than at 5mm SAR and it was reduced as the amounts of normal water increased after SAR application. 2. Chlorophyll content was higher at 10mm than at 5mm SAR application and increased as the amounts of normal water increased after SAR application in all crops. 3. Fresh weights of Chinese cabbage heads and radish roots and grain yield of soybean were higher at 10mm than at 5mm SAR, while they increased as the amounts of normal water increased at the same SAR level. 4. Changes in the mineral contents of plants were not consistent, while S content in radish and soybean increased as the amounts of SAR increased. 5. SAR lowered soil pH, while it increased soil N and S contents. Contents of soil organic matter, P, and exchangeable Ca, Mg, and K were not affected by the amounts of SAR.

• Journal of the Korea Organic Resources Recycling Association
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• v.3 no.1
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• pp.73-83
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• 1995

Effect of water treatment sludge(alum sludge) application on the growth of Korea local corn(Zeas Mays L.) was evaluated and phytotoxicity of alum sludge on the growth of corn roots and plant height was tested. Alum sludge itself and composted alum sludge compost were applicated by 1500kg/ha in the plot respectively. For the fertility test, yield, chlorophyll content, plant height of corn treated by alum sludge and composted alum sludge were better than that of control. No clear toxixity of alum sludge was found. At the food chemical analyses of seed corn, no obvious component variation were evaluated either. Test of pH effect on phytotoxicity of alum sludge on corns showed that plant height and root growth of corns were inhibited at low pH 4 solution. Phytotoxicity test of alum sludge extract which was extracted at various pH solutions, showed that growth and cations uptake inhibition were found the more serious the lower the pH. Plant height and root growth injury were serious at higher than 100ppm Al content solution.

• Korean journal of food and cookery science
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• v.28 no.5
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• pp.605-612
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• 2012

In comparison of the antioxidant activities by the different extraction methods of the domestic mung bean varieties, 'Geumsung', 'Dahyeon', 'Sohyeon', and 'Eohul', were soaked and dehulled, with the extracts obtained by using 80% ethanol at room temperature or heated. The mung bean hull was freeze-dried, ground, and passed through 100 mesh sieve. The moisture content of the hull powder ranged from 1.70 to 3.15%. The total dietary fiber content ranged from 84.42 to 88.47%, with the Sohyeon hull showing the highest value. The L value of Geumsung hull (62.93) was the highest, and the a value of Sohyeon hull (-1.69) was the lowest (p<0.05). The color difference was the highest in Dahyeon hull (42.52) and lowest in Geumsung hull (38.52). The Eohul hull's chlorophyll a and b contents were the highest with the 80% ethanol extract by heating (10.55 and 6.12 ${\mu}g/mL$, respectively), but the Sohyeon hull showed the highest in 80% ethanol extract at room temperature (7.63 and 3.93 ${\mu}g/mL$, respectively). The total phenolic and total flavonoid contents of the ethanol extracts were significantly different between the varieties regardless of the extraction temperature. The hull extract from the heating extraction was higher in Eohul than in other varieties, and was the lowest in the Dahyeon variety. The extract from Sohyeon hull at room temperature showed the highest contents of total phenolic and flavonoid contents. In the mung bean hull extract by heating, DPPH and ABTs radical scavenging activity ranged from 13.49-16.75% and 22.93-39.42% respectively and those at room temperature ranged from 39.86-41.88% and 49.71-70.92%. The reducing powers of extracts by heating ranged from 7.29 to 9.36 mg BHT/g hull, and at room temperature, they ranged from 19.90 to 20.97 mg BHT/g hull. Our results indicate that different extraction methods influenced antioxidant activities in the domestic mung bean varieties.