• 한국식품과학회지
• /
• 제3권1호
• /
• pp.29-36
• /
• 1971

감미종(甘味種) 소형(小型)고추인 Zairasisi를 저온저장(低溫貯藏)하니 종자(種子)의 갈변(褐變)이 급속(急速)히 일어났기에 여기에 주목(注目)하여 갈변기질(褐變基質)인 polyphenol 성분(成分)을 검색(檢索) 정량(定量)하고 이들 기질(基質)에 이르는 중간(中間) 대사(代謝) 성분(成分)의 변화(變化)를 측정(測定) 고찰(考察)하였다. (1) 종자(種子)의 polyphenol 성분(成分)으로서 chlorogenic acids가 검출(檢出)되었고 flavanol type polyphenols는 검출(檢出)되지 않았다. (2) Chlorogenic acid와 total polyphenol 함량(含量)은 저온처리(低溫處理)에 의하여 급증(急增)하고 갈변후(褐變後)는 감소하여 상온구(常溫區)보다 오히려 함량(含量)이 낮아졌다. (3) 중간대사(中間代謝) 성분(成分)인 phenylalanine, tyrosine, shikimic acid 함량(含量)은 저온처리(低溫處理)에 의하여 크게 증가(增加)하고 있으니 shikimate 계로(系路)가 활발(活潑)해지는 것으로 짐작된다. (4) 효소(酵素)에 대(對)한 특이적(特異的)인 조해제(阻害劑)와 호흡기질(呼吸基質)의 조직(組織) 호흡(呼吸)에 미치는 조해(阻害) 및 첨가(添加) 효과(效果)를 측정(測定)한 결과(結果) 저온처리(低溫處理)에 의하여 고추종자(種子)의 당(糖)은 EMP 계로(系路)를 활발히 움직이다가 이에 연결(連結)되는 TCA cycle에 jaming이 일어남에 따라 phosphoenolpyruvate와 erythrose-4-phosphate가 활발히 결합(結合)하고 shikimate 계로(系路)가 활발해져서 polyphenol 물질(物質)이 활발히 생합성(生合成)된다는 것을 추정(推定)할 수 있었다. (5) 저온처리(低溫處理)에 의하여 고추종자(種子)의 K ion 유출량(流出量)이 증가(增加)하였기에 갈변(褐變)에 따라 원형질막(原形質膜) 이상(異常)이 생긴 것을 짐작 할 수 있었다.

• 한국식품영양과학회지
• /
• 제32권4호
• /
• pp.501-505
• /
• 2003

프로폴리스를 이용하여 새로운 기능성 식품을 개발하기 위하여 프로폴리스 원괴와 그 추출물의 화학성분에 대하여 조사하였다. 일반성분은 조지방 86.41%, 가용성 무질소물 7.32%, 조단백질 2.71%, 회분 1.05% 및 조섬유 0.20%로 나타났다. 프로폴리스에 함유되어 있는 주요 무기성분은 Na(120.40 mg%), Ca(115.40 mg%) 및 K(105.87 mg%) 순으로 나타났으며, Ca 함량은 알콜 추출물보다 물 추출물에서 높게 나타났다. 유리당은 총 3종이 분리, 동정되었으며, 그 중 sucrose가 152 mg%로 높게 나타났고, glucose 114 mg% 및 fructose 6 mg%로 함유되어 있었다. 프로폴리스에 함유되어 있는 주요 아미노산으로는 lysine, cystine 및 glutamic acid로 나타났으며, 그 함량은 각각 395.29 mg%, 267.66 mg% 및 248.14 mg%로 나타났다. 지방산은 총 8종이 분리되었으며, 주요 지방산으로는 oleic acid (51.89%), myristic acid (20.86%) 및 palmitic acid (20.28%)로 나타났다. 프로폴리스의 flavonol 화합물을 분석한 결과 myricetin, quercetin, apigenin 및 kaempferol이 주요 flavonol화합물로 나타났으며, 총 flavonoid 함량은 다른 추출물보다 50% 에탄올 추출물에서 가장 높게 나타났다. 프로폴리스에 함유되어 있는 polyphenol 성분들은 gallic acid, chlorogenic acid, catechin, epigallocatechin gallate, epicatechin 및 epicatechin gallate로 확인되었다.

• 동아시아식생활학회지
• /
• 제23권6호
• /
• pp.768-777
• /
• 2013

Physicochemical characteristics and analysis of functional constituents of mulberry (Morus alba L.) fruit juices were investigated according to four different mulberry cultivars, including iksuppong, daeseongppong, cheongilppong and kwasangppong. Among the four mulberry cultivars examined, the small-sized cheongilppong had higher soluble solid content and lower titratable acidity, whereas the big-sized daeseongppong had higher titratable acidity and lower soluble solid content than other mulberry cultivars. Cheongilppong had higher contents of fructose and glucose, while daeseongppong had higher contents of citric and malic acids than the other mulberry cultivars. Kwasangppong had higher contents of two anthocyanins, cyanidin 3-glucoside and cyanidin 3-rutinoside, while cheongilppong had lower contents of two anthocyanins than the other mulberry cultivars. Daeseongppong had higher contents of resveratrols, flavonoids and moracin, whereas cheongilppong had higher levels of protocatechuic, chlorogenic and caffeic acids than the other mulberry fruits. Of the four mulberry cultivars, daeseongppong and cheongilppong had higher contents of GABA and DNJ, respectively, than the other mulberry cultivars. These results provide useful information to food technologists for the development and standardization of high quality mulberry juices as well as their processed foods.

• 한국자원식물학회지
• /
• 제31권6호
• /
• pp.704-713
• /
• 2018

Roasting has revealed coffee's potentials as a good source of bioactive compounds. This study was done to investigate the quantitative presence and activity of bioactive compounds including caffeine, chlorogenic acid (CGA), amino acids, and antioxidant capacity on Coffea arabica L. (Guatemala finca San Sebastian) and C. robusta L. (India Azad Hind). Analysis was performed on Green Bean (GB) Medium-Light (ML), Medium (ME) and Medium-Dark (MD) samples of both varieties. From the results, caffeine content was highest in ME samples of both varieties. GB samples of both varieties had high CGA content which decreased after increasing roasting time and temperature. Most amino acids in GB samples was highest, however, glutamic acid, valine, tyrosine, isoleucine, leucine and phenylalanine had highest quantitative increase in ME samples for both varieties. $IC_{50}$ of DPPH and ABTS radical scavenging activity was highest in ML samples of both varieties. $IC_{50}$ of reducing power and total phenolic content was highest in GB sample of both varieties but decreased after increasing roasting conditions. Generally Robusta had the highest quantity of bioactive compounds and antioxidant activity. From this study, the optimal roasting condition for coffee is ME above which there is a significant reduction of bioactive compounds and antioxidant activity.

• 한국약용작물학회지
• /
• 제14권2호
• /
• pp.82-86
• /
• 2006

Antioxidant activity of the extract fractions from leaves, stems, roots and flowers of Cirsium pendulum Fisch. was investigated. The results showed the greatest antioxidant activities in leaves by Rancimat, TBA and DPPH methods. Extracts of common thistle plants dose-dependently increased DPPH free radical scavenging activity, The extract from flowers and its hexane fraction showed the strongest antioxidant activity. HPLC analysis showed that BuOH fraction of the leaves had the highest amount of antioxidant chlorogenic and p-coumaric acids at 5.38 and 9.71 mg $100\;g^{-1}$, respectively. It implies that common thistle plants had potent antioxidant activity, and their activities were differently exhibited depending on plant part and solvent fraction.

• 생약학회지
• /
• 제43권4호
• /
• pp.302-307
• /
• 2012

The herb of Artemisia capillaris in Chinese medicine is used to treat hepatic diseases. In this research, qualitative analysis was performed using a UPLC/Q-TOF-ESI-MS/MS method for rapid identification of phenolic substances from A. capillaris: three caffeoylquinic acids (chlorogenic acid, 3,5-di-O-caffeoylquinic acid, and 4,5-di-O-caffeoylquinic acid), three flavonoids (hyperoside, isorhamnetin 3-O-robinobioside and quercetin) and three prenylated coumarins (6,8-diprenylumbelliferone, cedrelopsin and osthol) were identified. The three prenylated coumarins have not been reported from A. capillaris.

• Mycobiology
• /
• 제34권3호
• /
• pp.114-119
• /
• 2006

Biochemical variability among 20 Indian isolates of Sclerotinia sclerotiorum collected from different hosts/soil samples from different localities in India is reported. High Performance Liquid Chromatographic (HPLC) analysis of ethyl acetate fraction of culture filtrate, mycelia, sclerotia and sclerotial exudate showed $15{\sim}23$ peaks but only 11 could be identified. They were tannic, gallic, oxalic, caffeic, vanillic, ferulic, O-coumeric, chlorogenic, cinnamic, salicylic and gentisic acids. The amount of phenolic compounds varied among the culture filtrates, mycelia, sclerotia and sclerotial exudates of S. sclerotiorum.

• 동아시아식생활학회지
• /
• 제14권4호
• /
• pp.343-347
• /
• 2004

Abundant amounts of unripe apples, which were picked out before maturation, were abolished every year fer wastes. Since unripen apples are known to be rich in functional components, the ultimate objective of this research was to utilize them for food resources. The concentrations of polyphenols in hot water extract were the lowest, those in 75% acetone extract the highest. Concentrations of polyphenols in 5/30 sample were 3.5 times higher than those in 6/30 sample regardless of solvents used for extraction. The degree of condensation of flavanolic tannins in the peel was lower than those of the other parts, and the hot water extract was more condensed than the acetone extract. The amount of total flavonoids in the peel and 5/30 samples were 2.7 and 5.0 times higher than those of the flesh and 6/30 samples, respectively.

• 한국식품과학회지
• /
• 제38권2호
• /
• pp.183-187
• /
• 2006

감초와 녹차추출액에 신선절단한 감자를 침지하고 각각 LDPE 필름으로 포장하여 $4^{\circ}C$에서 저장하면서 저장 중 품질 특성을 조사하였다. 저장함에 따라 L value는 전반적으로 감소하는 경향을 나타냈으며 ascorbic acid를 함께 처리한 경우 하락 폭을 줄여 줄수 있었다. 저장 중 포장내의 이산화탄소 함량은 증가하였고 산소의 함량은 감소하였다. 저장 7일경까지는 녹차와 ascorbic과의 병용처리구를 제외하고는 큰 변화 가 없었으나 저장 14일경에는 모든 구에서 급격한 증가 현상을 보였다. Chlorogenic acid의 경우에도 총페놀과 유사한 경향을 나타내었으며 저장 기간 경과에 따라 직선적으로 증가하는 양상을 띠었다. PRO activity를 측정한 결과 저장 기간 경과에 따라 증가하고 있었으며 갈변이 효과적으로 저해되었던 저장 7일경의 감초 및 녹차에서 가장 낮은 활성을 나타내고 있으나 이후 14일경 갈변의 심화와 함께 효소의 활성도 함께 증가하는 것으로 나타났다. 따라서 천연물인 감초추출물과 ascorbic acid를 병용하여 사용할 경우 냉장저장 시 일정기간 동안 천연물을 이용한 갈변저해 및 품질유지가 가능할 것으로 판단된다.

• 생약학회지
• /
• 제52권4호
• /
• pp.257-266
• /
• 2021

This study evaluated several in vitro activities including the preliminary assessment of the anti-cancer, anti-inflammatory, and anti-diabetic effects of tree sprout extracts. Chlorogenic, caffeic, and p-coumaric acid contents in tree sprouts were analyzed using high-performance liquid chromatography and an ultraviolet detector. Among the studied tree sprout extracts, the ethanol (EtOH) extract of Rhus verniciflua exhibited the most potent anti-cancer effect by suppressing the cell viability of a human gastric adenocarcinoma cell line, with an IC₅₀ of 7.06 ㎍/mL. The EtOH extract of Morus alba (MAB) inhibited the secretion of nitric oxide (NO) at a concentration of 100 ㎍/mL, with an IC₅₀ of 83.44 ㎍/mL. Moreover, the EtOH extract of Securinega suffruticosa inhibited NO secretion with the lowest IC₅₀ of 54.42 ㎍/mL. The EtOH extract of Fraxinus mandschurica was the only extract with effective α-glucosidase inhibitory activity. The total content of chlorogenic, caffeic, and p-coumaric acids was the highest in MAB (14.63 mg/g ext.). In conclusion, the beneficial activities of the tree sprout extracts with high phenolic acid content were generally high. Our results provide a theoretical basis for the development of health-promoting supplements and functional foods.