• Journal of Veterinary Clinics
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• v.20 no.1
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• pp.91-95
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• 2003

It has been known that periods of absorption varies allografts or xenografts of transplantations of freeze-dried cortical bone(FDCB). In this study changes of absorption of FDCB in xenograft transplantations were evaluated according to extracted time with chloroform-methanol solution(CM sol.). The FDCB from pig was removed soft tissue by surgical knife. Fat of the FDCB was removed with treatments of CM sol. for 2, 6, and 10 days, then the treated FDCB was freeze-dried at $-80^{\circ}C$ and sterilized with ethylene oxide gas. The FDCB was transplanted to fifteen millimeter artificial-defected regions of 6 dogs on fibular diaphyses. This was biweekly examined by radiograph for 18 weeks. In result new bone formation with FDCB treated for 6 days was higher than the other bones treated for 2 and 10 days. Duration of absorption with FDCB treated for 6 days was longer than the others. The remain with FDCB treated for 10 days was more than the others.

• Korean Journal of Veterinary Service
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• v.16 no.1
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• pp.11-19
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• 1993

This experiment was carried out to detect the residues of sulfonamides in raw milk. Raw milks which does not contain sulfonamides was collected from one of the farm and fortified with 5 sulfonamides (sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfadimethoxine, sulfaqinoxaline). The sulfonamides in the fortified sample were extracted and detected by High Performance Liquid Chromatography. UV /vis detector was used in this experiment. The results obtained were summarized as follows : 1. Chloroform was good as a extracting solution. 2. 15.5% methanol in PDP as a mobile phase solution was best detective condition for SMR, SMT, SMM. But for SDM and SQN the best condition was 23% methanol. 3. The detectable limits of SMR, SMT, SMM were 2ppb. but SDM and SQN were 20ppb because of delayed retention time and relatively low recovery rate. 4. The peaks of SMR, SMT, SMM and SDM were erected at baseline and the apexes were sharp but SQN was round shape.

• Journal of Pharmaceutical Investigation
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• v.29 no.1
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• pp.55-60
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• 1999

We previously showed that a methanol extract of Paeoniae radix decreased total cholesterol level in rats with hyperlipidemia. In order to isolate the active ingredient(s), the methanol extract of Paeoniae radix was fractionated with chloroform/methanol(4:1) solution and isolate into soluble part and insoluble part of the the methanol extract. Above two parts were tested on the experimentally induced hypercholesterolemia in rats for the lowering effect of serum lipoprotein contents. Hyperlipidemia was induced on male Wistar rats by feeding high choleserol diet for 7 days. After oral administration of above samples for 4 weeks, serum lipid profile was verified on these rats by measuring total cholesterol, triglyceride, high density lipoprotein cholesterol and low density lipoprotein cholesterol. The chloroform/methanol(4:1) soluble part and insolule part showed lowering activity of total cholesterol level and triglyceride level at 4 week point significantly(p<0.01 and p<0.05) compare with the control group and the soluble part was more effective.

• Mycobiology
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• v.37 no.3
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• pp.203-206
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• 2009

To develop new antidementia nutraceuticals, a potent acetylcholinesterase (AChE)-inhibiting extract was screened from various extracts of nutritional mushrooms and lichens nutritional and its physicochemical properties were investigated. Among the several extracts tested, methanol extracts of Umbilicaria esculenta fruiting body showed the highest AChE inhibitory activity of 22.4%. U. esculenta AChE inhibitor was maximally extracted when fruiting bodies were treated with 80% methanol at $40^{\circ}C$ for 18 h. The methanol extracts contained 18.9% crude lipid, 18.8% crude protein, and 11.6% total sugar. In addition, they contained 444 mg/g glutamic acid, 44 mg/g histidine, and 41 mg/g aspartic acid. The methanol extracts were soluble in a solution of methanol and 20% dimethylsulfoxide, insoluble in n-hexane, chloroform, and water, and were stable at $20{\sim}60^{\circ}C$ and pH $1.0{\sim}5.0$ for 1 h.

• Journal of the Korean Applied Science and Technology
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• v.13 no.1
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• pp.87-98
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• 1996

In this study, benzoxazolo carbocyanine dye was used as sensitizer for photographic emulsion, and the photographic characteristics were examined. The basic properties of sensitizer such as stability in various solvents were examined. The sensitizer was very stable in methanol, acetonitrile, acetone, dimethylformamide, and chloroform solution. Absorption spectra of benzoxazolo carbocyanine dye $2.5{\times}10^{-6}M$ and $5{\times}10^{-6}M$ concentrations in 10% aqueous methanol solutions containing $10^{-2}M$ potassium chloride show the monomer-J-aggregation characteristics. Compared to the absorption peak of the monomer in pure methanol solution, the red shifts of the monomer-J-aggregate peaks of benzoxazolo carbocyanine dye of $2.5{\times}10^{-6}M$ and $5{\times}10^{-6}M$ concentrations in 10% aqueous methanol solutions containing $10^{-2}M$ potassium chloride are 34nm respectively, and the sensitizing peak of benzoxazolo carbocyanine dye in photographic emulsion has red shift of 34nm. Therefore, if was concluded that benzoxazolo carbocyanine dye can be used as green sensitizing dye for the spectral sensitization of photographic emulsion.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.6
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• pp.1266-1271
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• 2001

The cytotoxic effects of lipid extracts from soybean products were studied using K562 human leukemia cell, Yac1 mouse leukemia cell and S 180 mouse sarcoma cell. Total lipids from soybean powder, soybean curd residue and doenjang were extracted with chloroform/methanol (2 : 1) and water saturated butanol, consecutively, and fractionated into acetone supernatants (AS fraction) and acetone precipitates (AP fraction) by adding excess acetone. AS fraction of doenjang lipids showed the strongest cytotoxic effects on K562, Yac1 and S180 cancer cells, whereas each lipid fraction of soybean curd residue also showed relatively weak cytotoxic effects on cancer cells but soybean powder did not. AS and AP fractions of doenjang contained more free fatty acids than those of soybean curd residue and soybean. And when lipid fractions were digested with 0.4 N KOH/methanol, doenjang lipid fractions showed to contain some alkali-stable substances which showed positive reaction with ninhydrin solution on silica TLC separation.

• Microbiology and Biotechnology Letters
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• v.23 no.2
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• pp.229-235
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• 1995

The surface tension-decreasing biosurfactant was purified from Rhodotorula muciloginosa G-1. The purification procedure was the solvent extraction of culture broth. To ensure complete extraction, the sample was extracted twice with equal volume of ethylacetate. The crude solution was washed with n-hexane to remove unconsumed soybean oil. The crude sample of biosurfactant was applied to Silica gel column chromatography equilibrated with chloroform, and eluted with chloroform : methanol gradient. Serveral solvent system was used to developed the thin layer chromatography (TLC). The purified biosurfactant sample gave one spot (Rf 0.78). It was estimated that biosurfactant was glycolipid about having M.W.1,500 with standard of polyethyleneglycol by Sephadex LH-20 column chromatography.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.377-381
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• 2005

We tried to extract bone morphogenetic protein (BMP) from the freeze-dried bovine cortical bone (FBCB) for bone graft, which were defatted with chloroform-methanol for 20 days, freeze-dried at $-80^{\circ}C$ for 7 days and sterilized by ethylene oxide gas. Two kg of FBCB were pulverized in a wheel mill to $0.5-2.0mm^3$ cubic in size. The bone particles were demineralized in 0.6N HCI for 10 days at chloroform-methanol$4^{\circ}C$ and defatted with chloroform-methanol for 6 hours at room temperature, which was going to be defatting and demineralized cortical bone (DDM). For extracting BMP, DDM was agitated continuously through 72 hours with magnetic stirrer at $4^{\circ}C$ into 12 times of volume of 6 M guanidine hydrochloride (Gdn-HCl) solution containing proteinase inhibitors to protect BMP such as 2mM N-ethylaleimide, 1mM iodoacetic acid, 1mM phenylmethylsulfonyl fluoride and a sterilizer, 1mM sodium azide. The extraction procedure was repeated for three times. All extracted solution was centrifuged at 10,000 rpm for 30 min and then, the supernatant was dialyzed with 12 times of volume of deionized water at $4^{\circ}C$ for 24-72 hours, which cut off below 6,000-8,000 molecular weight. The dialyzed specimen contained crude-BMP was centrifuged, freeze-dried, and weighted. Through these processing, we could obtained $84.9\%$ as FBCB, $17.8\%$ as DDM and $0.71\%$ as crude-BMP from the wet cortical bone without cancellous bone, marrow and muscles. The crude-BMP were obtained $68.3\%$ from the first extraction, $29.6\%$ from secondary and $2.1\%$ from tertiary, respectively. It was suggested that high yield of crude-BMP migth be explained by three-time repetition of the extraction processing for crude-BMP with Gdn-Hcl sol.

• Journal of the Korean Applied Science and Technology
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• v.15 no.3
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• pp.27-31
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• 1998

Antioxodative substances in Mulberry leaves were examined. Antioxidative substances in Mulberry leaves were extracted by 80% methanol agueous solution. Antioxidative activity of extract was determined by examining hydrogen donating ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the inhibitory effect on the formation of the peroxide from Linoleic acid in the test tube at $50^{\circ}C$. Antioxidative substance were, then, separated and indentified by thin layer chromatography(TLC), UV-Vis spectrum and High performance liquid chromatography(HPLC) methods. Hydrogen donating ability on DPPH and antioxidative ability on linoleic acid of the extracted antioxidative substance were higher than those of 100ppm butylated hydroxy toluene(BHT). The extracted antioxidative substances were separated by TLC using ethylacetate : chloroform : formic acid : water(8 : 1 : 1 : 1 v/v) as a solvent, and a spot at Rf=0.35 was detected. The spot was scraped from the plate, and extrated by methanol. The extract was analyzed by UV-Vis spetra and HPLC, and chlorogenic acid was identified as a antioxidative substance.

• Korean Journal of Pharmacognosy
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• v.2 no.1
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• pp.39-40
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• 1971

An alkaloidal component was extracted from the root of Aconitum koreanum R. $R_{AYMOND}$ of the commercial origin. Crude extract was obtained in ca. 2% yield out of dried root with ether-methanol (10:1). White amorphous compound was precipitated out from the HCl solution upon the addition of ammonia water, and crude base was obtained by extraction of its mother liquid with chloroform. This crude base was purified by the alumina-column chromatographic method. A pure alkaloidal component(500mg) was yielded from its crude base. Its pure base comes as needle crystals melting at $250{\sim}252^{\circ}C$ (decomp.), $[\alpha]^{20}_D$ D+9.82 (methanol) and Anal. calcd. for $C_{19}H_{27}O_{8}N$.