Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Physical Proerties of Biosurfactant Produced from Rhodotorula muciloginosa

Rhodotorula muciloginosa G-1에서 생산되는 biosurfactant의 정제 및 물리적 성질

  • 이철수 (건국대학교 공과대학 미생물공학과) ;
  • 이병옥 (건국대학교 공과대학 미생물공학과) ;
  • 강상모 (건국대학교 공과대학 미생물공학과)
  • Published : 1995.05.01
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Abstract

The surface tension-decreasing biosurfactant was purified from Rhodotorula muciloginosa G-1. The purification procedure was the solvent extraction of culture broth. To ensure complete extraction, the sample was extracted twice with equal volume of ethylacetate. The crude solution was washed with n-hexane to remove unconsumed soybean oil. The crude sample of biosurfactant was applied to Silica gel column chromatography equilibrated with chloroform, and eluted with chloroform : methanol gradient. Serveral solvent system was used to developed the thin layer chromatography (TLC). The purified biosurfactant sample gave one spot (Rf 0.78). It was estimated that biosurfactant was glycolipid about having M.W.1,500 with standard of polyethyleneglycol by Sephadex LH-20 column chromatography.

Keywords

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