• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.212-212
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• 2017

This experiments were conducted to evaluate the influence of Chlorella culture solution on seed germination of perennial ryegrass seeds. Chlorella are known to contain different bioactive compounds. In present research work, Chlorella culture solution using liquid manure as medium have been used to study their effects on germination and root length. The study conducted a germination experiment in petri-dishes. Four treatments were compared: non-treated control treated with distilled water, Chlorella culture solution and Chlorella culture filtrate, and liquid manure. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination was the fast in Chlorella culture solution and the Chlorella culture filtrate treatment. Root length of perennial ryegrass seeds was long by 1~2cm in the Chlorella culture solution compared with no treated control. The germination index of perennial ryegrass seeds was high by 180~202% in the Chlorella culture solution treatment compared to no treatment. Chlorella culture solution and the Chlorella culture filtrate have shown stimulatory effects in germination and development of root. Use of Chlorella culture solution and the Chlorella culture filtrate can be recommended to farmers as a ecofriendly practice for better germination and growth. Present research work reveals that Chlorella contain certain growth promoting substances which enhances seed germination.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.1
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• pp.7-15
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• 2018

This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

• Korean Journal of Organic Agriculture
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• v.23 no.4
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• pp.939-950
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• 2015

The purpose of this study was to estimate the growth promoting effects and improvement of antioxidant activity of the soybean sprouts treated with Chlorella sp. culture solution. The soybean sprout treated with 0.1% and 0.2% Chlorella sp. culture solution was significantly increased the length (more than 43.0%), the thickness (more than 0.5~0.7 mm), fresh weight (more than 2.9~3.7 g) compared to non-treated control in vitro. In organic soybean sprouts farm, the 0.2% chlorella culture solution applied to mass culture of soybean sprout and the fresh weight of soybean sprouts increased by more than 25% and the yield was very high as 598.33% compared to untreated control. In addition of sensory test, there is no fishy odor and better crunchy texture and nutty flavor for the treatment soybean sprouts compared to untreated soybean sprouts. Particularly, free-radical scavenging activity (DPPH) and superoxide dismutase activity (SOD) of the soybean sprouts were significantly increased more than 26.1% and 40.4%, respectively by treated with 0.1% and 0.2% Chlorella culture solution. Consequently, the treatment of chlorella culture solution to grow soybean sprouts is also promoting quality and antioxidant activity as well as promoting the growth of sprouts. Therefore, chlorella is considered to be worth as functional materials for high-quality sprouts grown.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.4
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• pp.393-401
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• 2016

Anaerobic digestion is a collection of naturally occurring processes that convert organic matter and liquid residue, so-called digestate. The use of digestate biofertilizers is one of the important components of integrated nutrient management, as they are renewable sources of plant nutrients for sustainable agriculture. Seeds of Italian ryegrass (Lolium multiflorum L.) were germinated in different concentration of Chlorella in order to investigate it's the effect of Chlorella on growth parameters, seed germination and early growth. The experiment using plug tray was conducted at the green house placed in the Sangji University. The experiment consisted of nine treatments including different concentrations of Chlorella sp. culture solution and non-treated control. The germination percentage at the treatment with 25% Chlorella sp. culture solution was greater than that of control. The 50% concentration of Chlorella sp. culture solution was found to promote a better seedling growth in terms of shoot length, fresh weight and dry weight compared to the anaerobic digestate. Results showed that the best concentration of Chlorella culture solution was achieved by the 50% concentration of Chlorella culture solution treatment. As a conclusion, the application of Chlorella culture solution was found to be able to promote the germination and shoots growth of Italian ryegrass.

• Journal of Korean Society of Environmental Engineers
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• v.34 no.8
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• pp.523-527
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• 2012

This study was performed to determine optimum conditions of semi-continuous cultivation of chlorella sp. FC-21 cultivated under red light emitting diode (LED). Semi-continuous cultivation was conducted using red LED because red LED was found to be the best light source for chlorella sp. FC-21. During cultivation, phosphate and nitrogen were quickly diminished where cell concentration of chlorella was inversely proportional to the concentrations of phosphate and nitrogen in culture solution. To increase the period of dilution of culture solution, additional amounts of phosphate and nitrogen were inserted in the culture solution to increase the concentrations of phosphate and nitrogen. The cell concentrations of chlorella increased in the modified culture, but cell diameter was diminished as the dilution of culture was periodically conducted. When considered the cell concentration and cell diameter during the cultivation, amount of biomass produced was maintained constant.

• Korean Journal of Environmental Biology
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• v.26 no.3
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• pp.183-191
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• 2008

The effect of silver ion solution on the growth of Microcystis aeruginosa UTEX 2388 (cyanobacterium) and Chlorella sp. KCTC AG20136 (green alga) was investigated using separated and mixed culture in filtered natural water and BG11 medium. In separated culture, M. aeruginosa UTEX 2388 and Chlorella sp. KCTC AG20136 were found to be sensitive to 0.01 and 0.1 mg L$^{-1}$ of silver ion, respectively. Also, the silver ion concentrations for the growth inhibition of M. aeruginosa UTEX 2388 and Chlorella sp. KCTC AG20136 in the mixed culture were same in separated culture. Cyanobacteria were more sensitive to the silver ion solution than green algae. In bloom sample, the minimal inhibition concentration of silver ion solution for the low Chl-${\alpha}$ sample (110$\sim$190 ${\mu}g$ L$^{-1}$) and high Chl-${\alpha}$ sample (1,500$\sim$1,900 ${\mu}g$ L$^{-1}$) was about 0.1 and 3.0 mg L$^{-1}$, respectively. The silver ion concentration for the inhibition of algal bloom sample was affected by the algal biomass. In order to use silver ion solution for the control of algal bloom, the silver ion concentration must be determined in consideration of a minimal effect on the environment.

• Fisheries and Aquatic Sciences
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• v.2 no.2
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• pp.182-188
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• 1999

Production of extracellular polysaccharide by Monilinia fructigena in B-I medium containing cereals was higher than that in glucose medium. Productivities in B-I medium and glucose medium were 0.7g/l nd 0.2-03g/l respectively. The maximum content of polysaccharide occurred at the rising point from the lowest pH of culture. As the apparent viscosity of the polysaccharide solution increased, the flow Index (m) decreased, and the consistency Index (Kc) also increased. The polysaccharide solution was a typical pseudoplastic fluid. The mycelium was separated from the culture solution by $300\mu m$ mesh-filter and the polysaccharide was precipitated by adding 50% of ethanol (v/v). The amount of the polysaccharide removed from the filtrated solution was 0.45 g/l and the amount adhered to the mycelium was 0.25g/l. In experiments for investigating growth enhancement of rotifer (Brachionus plicatilis) by the polysaccharide, the dose of the polysaccharide was 1mg per 10,000 organisms of rotifer. Maximum specific growth rate of rotifer with feed consisting of sea Chlorella sp. and the polysaccharide was 1.095/day in the batch culture for 10 days. A semi-continuous culture was done for 30 days, the biomass of rotifer could be harvested twice. Maximum specific growth rate with sea Chlorella sp. and the polysaccharide was 0.734/day before the first harvest, and 1.685/day before the second harvest. Productivity was 38 $cells/ml\; \cdot\; day$ with sea Chlorella sp. and the polysaccharide.

• Korean Journal of Microbiology
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• v.1 no.1
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• pp.38-44
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• 1963

According to the experiment on pure-isolation and the related contaminants of Chlorella, the phenomena of the ecological distributions of Chlorella in Korea have been manifested in several areas and also the aim that in going to do culture, biological and physiological study of Chlorella is carried out. Contaminants very oftenly occupied on the colony of the strains taken in order to fulfil pure-isolation of Chlorella, but in accordance with being piled up the minute research on this subject, I can obtain the desirable results as follows: 1. For the pure-isolation, the duration chose the time from May to September 1957 so that may easily isolate from contaminant water with utilizing the antibiotic substances. 2. To take long time, 36-48 hours until growth of nascent through the non-sporulated, it originates from the difference of the cultured media. In addition to the above mention, the mechanism of growth until nascent through the sporulated must not always require the ligh. However the supply of metabolic energy depend upon its nutritional conditions per phase. 3. The culture of Chlorella should be based on the lower culturing except adding especial conditions such as reagent concentration of media, artifical shake of media and other facts due to the natural conditions. And also these strains grew not only in distilled water but 2% NaCl solution without any abnormality in cell it self. I, therefore, guess it is possible to culture in sea-water under phasic environment. 4. In the experiment of ammonia detection, it is caused by the sampling surroundings to contain the minute quantity of ammonia in strain No. M 918; that is the place to be plenty of Carbohydrate on behalf of protein. 5. To compare the absorption curve of chlorophyll of higher plant with that of Chlorella, the absorption zone made mostly the Same ones each other but a little absorption grade dose not clearly appear. The colony which formed giant type grows with intensive colour and green band on surrounding of the colony and after that it was changed into all the green colour and developed up to end. 6. At first phase for a week, the development of Chlorella suspends the normal condition as in vivo but after a few days, the colour of chlorophyll gradually changed into blue-yellow which secrete the mucous substances on the agar media. The cell was flew out the contained substances itself on leaving the cell wall only, or the various micro-organism diffused on the outer-region of the cell.

• Korean Journal of Environmental Agriculture
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• v.41 no.4
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• pp.282-287
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• 2022

BACKGROUND: Green algae (Chlorella spp.) has been widely used as a biofertilizer to improve the crop yield and quality. However, available information is not enough to verify the mechanism of green algae's beneficial impact on strawberry. This study was conducted to investigate the effect of Chlorella fusca CHK005 application on the growth and nutrient status of strawberry plant and fruit characteristics. METHODS AND RESULTS: A total of 800 seedlings of strawberry variety 'Kuemsil' were planted. Once a week, C. fusca culture solution (1.0 × 10⁷ cells mL^-1) was applied into soil via irrigation in four different concentrations: no application (control), 1/1000 times (× 0.5), 1/500 times (× 1), and 1/250 times (× 2). Result showed that growth of strawberry plant was enhanced by Chlorella application and the highest impact on fresh weight (FW) and chlorophyll content of the plants were observed in × 2 treatment, followed by × 1, × 0.5, and control treatments. The phosphorus (P) concentration in the plant was significantly higher in × 1 and × 2 treatments compared to control. In case of fruit quality, sugar content (°Brix), hardness, and FW were lowest in control, but these values increased as application levels of Chlorella were higher. Also, P and K contents in the fruits increased with increasing the application levels and significant correlation between P content and oBrix in the fruits was found. CONCLUSION(S): Overall, Chlorella application seemed to improve plant growth and fruit quality by increasing the utilization efficiency of P and K in strawberries.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.4
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• pp.427-435
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• 2016

The effects of salt solution and chlorella on the quality of canned oyster, Crassostrea gigas, were evaluated to obtain basic data regarding the processing of two canned oyster products. In canned oyster processing, the shucked oyster meat was steamed for 20 min and then drained. Then, each can (301-3) was filled with 90 g boiled oyster in 60 mL 1.5% salt solution for the control samples or 30 mL 1.5% salt solution and 30 mL chlorella culture medium for the experimental samples. All canned products were sealed using a vacuum seamer and then sterilized to Fo values of 6-12 min in a steam retort system at 118℃. The viable bacteria count, proximate composition, pH, salinity, yield, volatile basic nitrogen (VBN), amino-nitrogen, thiobarbituric acid (TBA), mineral, color value, free amino acid levels, hardness, and sensory evaluation of the two canned products were measured under various sterilization conditions. There were no significant differences in the physical or chemical factors and little difference in the overall acceptance of the control and experimental samples.