Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Application Effect of Chlorella Fusca CHK0059 as a Biofertilizer for Strawberry Cultivation

딸기재배를 위한 생물비료로 Chlorella fusca CHK0059의 시비효과

  • Young-Nam Kim (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Jun Hyeok Choi (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Song Yeob Kim (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Hyeonji Choe (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Yerim Shin (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Young-Eun Yoon (Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Keum-Ah Lee (Institute of Agriculture and Life Science (IALS), Gyeongsang National University) ;
  • Min-Jeong Kim (Organic Agriculture Division, National Academy of Agriculture Science, Rural Development Administration) ;
  • Yong Bok Lee (Division of Applied Life Science (BK21), Gyeongsang National University)
  • 김영남 (경상국립대학교 응용생명과학부(BK21)) ;
  • 최준혁 (경상국립대학교 응용생명과학부(BK21)) ;
  • 김송엽 (경상국립대학교 응용생명과학부(BK21)) ;
  • 최현지 (경상국립대학교 응용생명과학부(BK21)) ;
  • 신예림 (경상국립대학교 응용생명과학부(BK21)) ;
  • 윤영은 (경상국립대학교 농업생명과학연구원(IALS)) ;
  • 이금아 (경상국립대학교 농업생명과학연구원(IALS)) ;
  • 김민정 (농촌진흥청 국립농업과학원 유기농업과) ;
  • 이용복 (경상국립대학교 응용생명과학부(BK21))
  • Received : 2022.11.23
  • Accepted : 2022.12.11
  • Published : 2022.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

BACKGROUND: Green algae (Chlorella spp.) has been widely used as a biofertilizer to improve the crop yield and quality. However, available information is not enough to verify the mechanism of green algae's beneficial impact on strawberry. This study was conducted to investigate the effect of Chlorella fusca CHK005 application on the growth and nutrient status of strawberry plant and fruit characteristics. METHODS AND RESULTS: A total of 800 seedlings of strawberry variety 'Kuemsil' were planted. Once a week, C. fusca culture solution (1.0 × 107 cells mL-1) was applied into soil via irrigation in four different concentrations: no application (control), 1/1000 times (× 0.5), 1/500 times (× 1), and 1/250 times (× 2). Result showed that growth of strawberry plant was enhanced by Chlorella application and the highest impact on fresh weight (FW) and chlorophyll content of the plants were observed in × 2 treatment, followed by × 1, × 0.5, and control treatments. The phosphorus (P) concentration in the plant was significantly higher in × 1 and × 2 treatments compared to control. In case of fruit quality, sugar content (°Brix), hardness, and FW were lowest in control, but these values increased as application levels of Chlorella were higher. Also, P and K contents in the fruits increased with increasing the application levels and significant correlation between P content and oBrix in the fruits was found. CONCLUSION(S): Overall, Chlorella application seemed to improve plant growth and fruit quality by increasing the utilization efficiency of P and K in strawberries.

Keywords

Acknowledgement

This work was carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ015641022022)" Rural Development Administration, Republic of Korea.

References

  1. Abdel-Raouf N, Al-Homaidan AA, Ibraheem IBM (2012) Agricultural importance of algae. African Journal of Biotechnology, 11, 11648-11658. https://doi.org/10.5897/AJB11.3983. 
  2. Kawalekar JS (2013) Role of biofertilizers and biopesticides for sustainable agriculture. Journal of Bio Innovation, 2, 73-78. 
  3. Thomas L, Singh I (2019) Microbial biofertilizers: Types and applications, in: Giri B, Prasad R, Wu QS, Varma A, Biofertilizers for Sustainable Agriculture and Environment. Soil Biology, pp. 1-19, Springer, Cham. https://doi.org/10.1007/978-3-030-18933-4_1. 
  4. Ann SW, Lee JM, Cho YK (2020) Perilla leaf fertilization effect of fertilizer by Chlorella and seafood by-product fermentation. Journal of Environmental Science International, 29, 423-434. https://doi.org/10.5322/JESI.2020.29.4.423. 
  5. Lee SM, Ryu CM (2021) Algae as new kids in the beneficial plant microbiome. Frontiers in Plant Science, 12, 599742. https://doi.org/10.3389/fpls.2021.599742. 
  6. Kang HK, Choi HC, Kim DW, Hwangbo J, Na JC, Bang HT, Kim DW, Kim MJ, Mushtaq MMH et al. (2013) Effect of dietary chlorella supplementation on growth performance, immune response, and intestinal microflora concentration of broiler chickens. Korean Journal of Poultry Science, 40, 271-276. https://doi.org/10.5536/KJPS.2013.40.3.271. 
  7. Bai SC, Cha YT (1997) Comparison of growth and body composition in olive flounder larvae (Paralichthys olivaceus) feddomestic experimental and imported commercial microparticulated diets. Journal of Aquaculture, 10, 87-95. 
  8. Goncalves AL (2021) The use of microalgae and cyanobacteria in the improvement of agricultural practices: a review on their biofertilising, biostimulating and biopesticide roles. Applied Sciences, 11, 871. https://doi.org/10.3390/app11020871. 
  9. Kim MJ, Shim CK, Kim YK, Jee HJ, Park JH, Hong SJ (2012) Effect of green algae, Chlorella fusca on improving seed germination and seedling growth in vegetable crop and minor cereal. Korean Journal of Organic Agriculture, 2012, 491-492. 
  10. Kim MJ, Shim CK, Kim YK, Ko BG, Park JH, Hwang SG, Kim BH (2018) Effect of biostimulator Chlorella fusca on improving growth and qualities of Chinese chives and spinach in organic farm. Plant Pathology Journal, 34, 567-574. https://doi.org/10.5423/PPJ.FT.11.2018.0254. 
  11. Faheed FA, Fattah ZA (2008) Effect of Chlorella vulgaris as bio-fertilizer on growth parameters and metabolic aspects of lettuce plant. Journal of Agriculture and Social Science, 4, 165-169. 
  12. Bumandalai O, Tserennadmid R (2019) Effect of Chlorella vulgaris as a biofertilizer on germination of tomato and cucumber seeds. International Journal of Aquatic Biology, 7, 95-99. https://doi.org/10.22034/ijab.v7i2.582. 
  13. Kim MJ, Shim CK, Ko BG, Kim J (2020) Effect of the microalga Chlorella fusca CHK0059 on strawberry PGPR and biological control of fusarium wilt disease in non-pesticide hydroponic strawberry cultivation. Journal of Microbiology and Biotechnology, 30, 708-716. https://doi.org/10.4014/jmb.2001.01015. 
  14. Khalid AAH, Yaakob Z, Abdullah SRS, Takriff MS (2019) Analysis of the elemental composition and uptake mechanism of Chlorella sorokiniana for nutrient removal in agricultural wastewater under optimized response surface methodology (RSM) conditions. Journal of Cleaner Production, 210, 673-686. https://doi.org/10.1016/j.jclepro.2018.11.095. 
  15. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24, 1-15.  https://doi.org/10.1104/pp.24.1.1
  16. Lichtenthaler HK (1987) Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 148, 350-382. https://doi.org/10.1016/0076-6879(87)48036-1. 
  17. Paudel Y, Pradhan S, Pant B, Prasad B (2012) Role of blue green algae in rice productivity. Agriculture and Biology Journal of North America, 3, 332-335.  https://doi.org/10.5251/abjna.2012.3.8.332.335
  18. Renuka N, Prasanna R, Sood A, Ahluwalia AS, Bansal R, Babu S, Singh R, Shivay YS, Nain L (2016) Exploring the efficacy of wastewater-grown microalgal biomass as a biofertilizer for wheat. Environmental Science and Pollution Research, 23, 6608-6620. https://doi.org/10.1007/s11356-015-5884-6. 
  19. Trejo-Tellez LI, Gomez-Merino FC (2014) Nutrient management in strawberry: Effects on yield, quality and plant health, in: Malone N, Strawberries: Cultivation, Antioxidant Properties and Health Benefits. pp. 239-267, Nova Science Publishers, Inc., NY, USA. 
  20. Moor U, Poldma P, Tonutare T, Karp K, Starast M, Vool E (2009) Effect of phosphite fertilization on growth, yield and fruit composition of strawberries. Scientia Horticulturae, 119, 264-269. https://doi.org/10.1016/j.scienta.2008.08.005. 
  21. Estrada-Ortiz E, Trejo-Tellez LI, Gomez-Merino FC, Nunez-Escobar R, Sandoval-Villa M (2012) Phosphite on growth and fruit quality in strawberry. Acta Horticulturae, 947, 277-282. https://doi.org/10.17660/actahortic.2012.947.35