• 한국작물학회지
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• 제45권2호
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• pp.139-142
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• 2000

The experiment was carried out to study the variations and geographical distribution of $\beta$-amylase isozyme by isoelectric focusing (IEF) within Korean, Chinese and Japanese soybean land races. In pH 3-10 gel of IEF, the amylase of soybean accessions was separated into low pI group isozymes (TEX>${$Sp_1$}^b$) and high pI group isozymes(${$Sp_1$}^a$). In pH4-6.5 gel, isoelectric points were at 5.07, 5.15, 5.25, 5.40, and 5.94, and h, j, and k bands also were found. The distribution of Sp$_1$$^{a}$ allele (high pI type) was 29.3% in soybean accessions from Korea, 10.1 % in those from China, and 6.9% in Japanese accessions. The percentage of ${$Sp_1$}^a$) allele was the highest in soybean accessions from Kyungsang province (35 %) in Korea, then central China (32 %) in China, and Honshu (10%) in Japan.

• 한국토양비료학회지
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• 제29권3호
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• pp.255-263
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• 1996

풍농비료(豊農肥料)의 분상(粉狀) 용성인비(熔成燐肥)를 대조로 하고, 입상화 제조시 상이한 첨가물질에 의해 제조된 분상(粉狀) 용성인비(熔成燐肥) 3제품(製品)(경기화학(京畿化學) 풍농비료(豊農肥料), 수입(輸入) 중국산(中國産))의 시용이 대두(大豆)의 종실수량(種實收量)에 미치는 영향을 비교 검토하였다. 참고로 수용성 인산질비료의 과석(過石)과 수용성과 구용성 인산이 반반씩 함유된 용과린(熔過燐)도 처리하여 제주도(濟州道) 화산회토(火山灰土)(남원통(南元統)) 포장(圃場)에서 비효시험을 실시한 결과를 요약하면 다음과 같다. 1. 대두(大豆)의 종실수량(種實收量)은 풍농비료(豊農肥料)의 분상(粉狀) 용성인비(熔成燐肥) 제품(製品)이 가장 많았고 그 다음이 수입 중국산 입상 용성인비 제품이었으며 경기화학의 입상 용성인비 제품의 수량이 가장 낮은 경향이었으나 분상(粉狀) 용성인비(熔成燐肥) 제품간(製品間)의 수량은 통계적 유의성(有意性)이 없었다. 2. 수량(收量)과 수량구성요소간(收量構成要素間)에는 일정한 경향을 찾을 수 없었으나 수량(收量)이 많았던 처리를 중심으로 해서 보면 주경절수(主莖節數), 분지수(分枝數) 및 꼬투리수의 영향(影響)이 큰 것으로 보여졌다. 3. 수확기(收穫期) 식물체(植物體) 지상부(地上部)(줄기 + 협실(莢實))의 인산함양(燐酸含量)이 생육기간중 식물체 경엽중의 인산함량 보다 높았으며, 수확기(收穫期)의 인산흡수풍(燐酸吸收豊)과 인산이용율(燐酸利用率)이 대두의 수량증수와 비슷한 경향을 보여 수량(收量)에 가장 큰 영향을 주었던 것으로 판단되었다. 4. 시기별(時期別) 토양중(土壤中) pH변화(變化)는 인산질비료(燐酸質肥料)를 시용하므로써 증가추세를 보였고, 수입(輸入) 중국산(中國産) 입상용성(粒狀熔成) 인비제품(燐肥製品)의 pH가 국내산 입상 용성인비 제품 보다 높았으나 과석(過石)과 비교해서는 서로 비슷하였으며 토양중(土壤中) 유효이산함량(有效理酸含量)은 풍농비료(豊農肥料)의 입상(粒狀) 용성인비(熔成燐肥) 제품(製品)이 수입 중국산 입상 용성인비 제품 보다 오히려 더 높았다.

• 한국식품영양과학회지
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• 제31권1호
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• pp.17-20
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• 2002

경남 도내에서 유통 중인 국내산 참기름과 중국산 참기름 각각 30건과 콩기름 및 옥수수기름 등의 식용유지를 첨가하여 변조한 참기를 12건에 대한 지방산 함량을 GC에 의한 정량법으로 분석하여 다음과 같은 결과를 얻었다. 국내산 참기름의 주요 지방산 평균 함량은 palmitic acid 8.26$\pm$0.04%, stearic acid 4.57$\pm$0.05%, oleic acid 39.25$\pm$0.05%, linoleic acid 46.82$\pm$0.07, linolenic acid 0.52$\pm$0.02%, arachidic acid 0.58$\pm$0.03% 등으로 나타났으며, 중국산 참기름은 palmitic acid 9.24$\pm$0.03%, stearic acid 5.11$\pm$0.04%, oleic acid 38.65$\pm$0.04%, linoleic acid 46.02$\pm$0.06%, linolenic acid 0.42$\pm$0.04%, arachidic acid 0.56%$\pm$0.04% 등으로 각각 나타났다. 국내산 참기름은 포화 지방산인 palmitic acid와 stearic acid가 중국산 참기름보다 적게 함유되어 있었고 불포화 지방산인 linolenic acid는 반대로 많이 함유되어 있었다. 국내산 참기름에 콩기름을 0%, 3%, 9%씩 첨가했을 때 불포화지방산인 linolenic acid가 각각 0.52$\pm$0.02%, 0.73$\pm$0.05%, 1.25$\pm$0.04%로 현저히 증가되었으며, 옥수수기름을 0%, 5%, 9%씩 첨가했을 때는 불포화지방산인 linoleic acid의 함량이 각각 46.82$\pm$0.07%, 50.22$\pm$0.06%, 51.14$\pm$0.05%로 증가됨이 확인되었다. 참기름의 산지별 확인과 식용유지를 첨가한 변조참기름에 대해서는 각각의 지방산 함량에서 특이성을 보이고 있으나 일부지역에서 구입한 국내산과 중국산 참기름에서 유사한 결과치를 보여 앞으로 국내에서 유통되는 참깨나 참기름에 대하여 더 많은 연구가 필요할 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권4호
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• pp.555-564
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• 2002

Twenty Landrace and twenty Min piglets, with an average initial body weight of 22.4 kg, were randomly divided into 5 groups with 4 animals per group, within each of the breeds. The piglets were housed in individual concrete pens. Each group of the piglets was fed one of 5 diets. The diets contained either 20% raw Argentine soybeans, 20% processed Argentine soybeans ($118^{\circ}C$ for 7.5 min.), 20% raw Chinese soybeans, 20% processed Chinese soybeans ($118^{\circ}C$ for 7.5 min.) or no soybean products (control diet). Faecal samples were collected on days 6, 7 and 8 of the treatment period. Digestibilities of dietary nutrients were determined with AIA (acid insoluble ash) as a marker. After a 17 day treatment, three piglets were killed from each of the groups. Tissue samples of small and large intestine for light and electron microscopy examination were taken immediately after the opening of abdomen. Then, the weight or size of relevant organs was measured. The results show that the digestibilities of dry matter (DM), crude protein (CP) and fat were higher in Min piglets than in Landrace piglets (p<0.05). The diets containing processed soybeans had a significant higher CP digestibility than the control diet and the diets containing raw soybeans (p<0.05). Landrace piglets had heavier and longer small intestines, heavier kidneys and a lighter spleen than Min piglets (p<0.05). The pancreas of the animals fed the diets containing processed soybeans was heavier than that of the animals fed control diet (p<0.05) and the diets containing raw soybeans. But, the differences between raw and processed soybean diets were not significant. A significant interaction (p<0.05) between diet and pig breed was observed in weight of the small intestine. The Landrace piglets increased the weight in their small intestine when they were fed the diets containing soybeans. In the light micrographs and electron scanning micrographs, it was found that the villi of small intestinal epithelium of animals (especially Landrace piglets) fed the diets containing raw Chinese soybeans were seriously damaged. The transmission electron micrograph showed that a lot of vesicles were located between the small intestinal microvilli of these piglets. The histological examination also indicated that the proportion of goblet cells in villi and crypts in the piglets consuming the control diet was significantly lower (p<0.01 and p<0.02, respectively) than those of the animals consuming the diets containing raw or processed soybeans.

• 동아시아식생활학회지
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• 제8권4호
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• pp.360-378
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• 1998

Imwonshibyukji was a copying manuscript written around 1825, the 27th year of king Soonjo of the Josun Dynasty. It was a massive home encyclopedia of total 52 books and 100 volumes. The unpublished 'Jeongjoji' of its 17-20th volumes analyzed the cooking methods on the steamed rice and gruel and studied the degree of their usefulness and medical values in the light of the cooking process science. The cooking method on the steamed rice was composed of 14 items, and the general introduction outlined 6 kinds of the rice. These were all quoted from Chinese literatures. The 11 items present the methods on the general production of the rice, 2 on the cooking process, and 1 on the preservation. The main material of the rice was rice, and others were naked barley, prosomillet, foxtail millet, glutinous millet, etc. , and the secondary materials were glutinous rice, small red bean, black soybean, potato, bamboo seed, jujube, taro, gaertner, chestnut powder, persimmon power, julib(Zizania caduciflora), mangcho(Erigeron canadensis), namchok(Nandina domestica), licorice root, nitrous, peach, palmicha(schizandra, jinseng, cheonmoondong(Asparagus), and honey are mixed), etc. The literatures quoted in the rice were all 33, in which 23 were Chinese (69.7%) and 10 were Korean (30.3%). In the case of gruel, the cooking methods on the general gruel were described in 41 items, and on the gruel for a medical treatment were in 48 items, in which there was not a cooking method on the gruel but only its medical values were presented. The materials used for the general gruel were approximately 60 kinds: rice, glutinous rice, munbean, job's tears, rye, soybean, black sesame seed, antler of cervidae, chicken, crucian carp, and various medical materials, etc. The gruel was mainly used for protection and medical treatment, and partly for food for hungry people. The literatures quoted in the gruel were total 57, in which 26 were Korean(45.6%), and 31 were Chinese (54.4%). It can be their characteristics that Almost all of the Chinese literatures on the methods of the steamed rice and gruel do not exist.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2018년도 추계학술대회
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• pp.9-12
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• 2018

China is an agricultural country. Rice, maize and soybean are major crops for national economy and people's livelihood. These three crops are also the pillar industry in northeast China, especially in Heilongjiang province.

• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1164-1173
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• 2006

Cellulase from Aspergillus niger, (${\alpha}$-amylase from Bacillus sp. and protease from Bacillus globigii were used as enzyme sources in this study to examine how their respective substrates protect them in two kinds of simulated gastrointestinal tract digesting processes. Avian total digest tract simulation test showed that filter paper, Avicel and cellulose resulted in 7.7, 6.4 and 7.4 times more activity than of unprotected cellulose, respectively. Protease with addition of casein, gelatin or soybean protein showed no significant protection response. Starch protected amylase to be 2.5 times activity of the unprotected one. Monogastric animal total tract digestion simulation test showed that filter paper, Avicel and cellulose resulted in 5.9, 9.0 and 8.8 times activity of unprotected cellulase, respectively. Casein, gelatin and soybean protein resulted in 1.2, 1.3 and 2.0 times activity of unprotected protease, respectively. Starch did not protect amylase activity in monogastric animal total tract simulation. Protection of mixed enzymes by substrates in two animal total tract simulation tests showed that filter paper in combination with soybean protein resulted in 1.5 times activity of unprotected cellulose, but all substrates tested showed no significant protection effect to protease. Soybean protein and starch added at the same time protected the amylase activity to be two times of the unprotected one. Test of non-purified substrate protection in two animal total digest tract simulation showed that cellulase activity increased as BSA (bovine serum albumin) concentration increased, with the highest activity to be 1.3 times of unprotected enzyme. However, BSA showed no significant protection effect to protease. Amylase activity increased to 1.5 times as BSA added more than 1.5% (w/v). Cellulase activity increased to 1.5 times as soybean hull was added higher than 1.5%. Amylase had a significant protection response only when soybean hull added up to 2%. Protease activity was not protected by soybean hull to any significant extent.

• 한국작물학회지
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• 제44권3호
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• pp.256-262
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• 1999

To evaluate the genetic diversity and structure of the South Korean soybean population, 233 landraces collected in various regions of the country were surveyed for 15 allozyme loci and one protein locus. The South Korean population was fixed or nearly fixed at seven of the 16 loci tested. The number of alleles per locus was 2.06 and Nei’s gene diversity was 0.194. These values were lower than the values for the same 16 loci previously reported for the Japanese and Chinese populations. The differences among eight regional groups were not so marked, with only 7.2% of the total variation arising from regional differentiation. Three southern regional groups (Chollabuk-do, Chollanam-do and Kyong-sangnam-do) exhibited a relatively high variability because of frequent occurrence of alleles characteristic of the Japanese population. A marked difference was found in allelic frequencies at the Dial locus between large-seeded landraces and small-seeded ones, suggesting that the latter, which are used mainly for bean sprouts, had been established independently of the former, which are used mostly for soy sauce and cooking with rice. Not only the region but also the usage as food materials should therefore be taken into consideration in designing an efficient collection and preservation method for the Korean soybean landraces.

• Animal Bioscience
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• 제35권12호
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• pp.1892-1903
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• 2022

Objective: A series of experiment were conducted to evaluate the effects of replacing a part of soybean meal (SBM) at 6% of broiler diets with fermented soybean meal (FSBM) obtained by single or two-stage fermentation by measuring growth performance, antioxidant activity in the jejunum and distal intestinal microflora. Methods: Soybean meal samples were prepared by single-stage fermentation using Bacillus velezensis (Bv) (FSBM_B), or Lactobacillus spp. (as commercial control) (FSBM_L). Additional SBM sample was prepared by two-stage fermentation using Bv and subsequently using Lactobacillus brevis ATCC 367 (Lb) (FSBM_B+L). Enzyme activity, chemical composition, trichloroethanoic acid-nitrogen solubility index (TCA-NSI) and antioxidant activity were measured. Then, in an in vivo study, 320 Ross308 broilers were divided into four groups with ad libitum supply of feed and water. Four groups were fed either a corn-soybean meal diet (SBM), or one of fermented SBM diets (FSBM_B+L, FSBM_B, and FSBM_L). Growth, serum characteristics, microflora, and the mRNA expression of selected genes were measured. Results: Compared to SBM, FSBM_B+L contained lower galacto-oligosaccharide, allergic protein, and trypsin inhibitor, and higher TCA-NSI by about three times (p<0.05). Reducing power and 1,1-diphenyl-2-picrylhydrazyl free radical scavenging ability correlated positively with the TCA-NSI content in FSBM. Growth performances were not significantly different among four groups. In jejunum of 35-day-old broilers, partial replacement of SBM by FSBM_B+L increased the activity of superoxide dismutase and catalase (CAT), and the FSBM_B group had the highest catalase activity (p<0.05). Partial replacement of SBM by FSBM increased relative mRNA expressions of nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and peptide transporter 1 (PepT1) (p<0.05); however, FSBM_B+L increased CAT mRNA level to 5 times of the control (p<0.05). Conclusion: Using Bv- and Lb-processed SBM through two-stage fermentation to partially replace 6% of diets will improve the gut's antioxidant activity under commercial breeding in broilers.

• 한국환경농학회지
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• 제15권3호
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• pp.341-347
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• 1996

인공산성비 다음에 처리된 일반비가 배추, 무, 콩의 생육 및 토양 화학성에 미치는 영향을 구명하기 위하여 인공산성비(pH 2.7)를 5mm, 10mm를 살포한 후 일반비를 각각 0, 5, 10mm 씩 살포하는 처리를 3일 간격으로 배추와 무는 20회, 콩은 42회 실시하여 작물의 가시적 피해증상, 엽록소 함량, 수량 및 수량과 관련된 특성, 식물체 및 토양의 무기 양분 함량 등을 분석 조사한 결과는 다음과 같다. 1. 배추, 무, 콩의 가시적 피해정도는 인공산성비 처리량이 많을수록 심한 편이었고, 일반비의 처리량이 많을수록 감소되었다. 피해증상은 잎이 담갈색, 연록색으로 변하고, 황백색의 괴사 반점이 생기면서 잎 가장자리가 위축되었다. 2. 엽록소 함량은 배추, 무, 콩 모두 인공산성비 5mm보다 10mm 처리에서 높았고, 인공산성비 처리 후 일반비 처리량이 많을수록 높았다. 3. 배추, 무, 콩 등의 수량은 인공산성비 처리량이 많을수록, 또 인공산성비 살포 후 일반비 처리량이 많을수록 증가되었다. 4. 식물체중 무기양분 함량은 일반비 처리량에 따른 일정한 경향이 없었으나, 무와 콩의 황 함량은 인공산성비의 처리량이 많을수록 높았다. 5. 시험 후 토양의 화학성은 배추, 무, 콩 모두 토양 pH는 다소 감소하였고, 질소와 황 함량은 증가되었으며, 유기물, 인산, 치환성 칼슘, 마그네슘 및 칼리 함량 등은 일정한 경향이 없었다.