• Title/Summary/Keyword: Chinese science

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${\beta}$-1,4-Xylosidase Activity of Leuconostoc Lactic Acid Bacteria Isolated from Kimchi (김치에서 분리된 Leuconostoc 속 젖산균의 ${\beta}$-1,4-xylosidase 효소생산 특성)

  • Jang, Mi-Hee;Kim, Myoung-Dong
    • Korean Journal of Food Science and Technology
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    • v.43 no.2
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    • pp.169-175
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    • 2011
  • The ${\beta}$-xylosidase (EC 3.2.1.37) production capabilities of lactic acid bacteria in the genus Leuconostoc, isolated from a variety of kimchi (fermented vegetables), were examined. The intracellular levels of ${\beta}$-xylosidase were similar to the extracellular levels, when most Leuconostoc lactic acid bacteria were grown in a medium containing xylose as the carbon source. Intracellular ${\beta}$-xylosidase with a maximum activity of $1.2{\pm}0.1units/mL$ (mean${\pm}$standard error) was obtained from Leuconostoc lactis KCTC 13344, which was isolated from fermented Chinese cabbage. The optimum reaction conditions for Leu. lactis KCTC 13344 ${\beta}$-xylosidase activity were pH 6.0 and $30^{\circ}C$, and the addition of most divalent cations, except zinc, to the reaction mixture resulted in a slight increase in enzyme activity. Compared with a media containing other carbon sources, the ${\beta}$-xylosidase activity was 5 times higher when Leu. lactis KCTC 13344 was grown in a medium containing xylose as carbon source. Zymographic analysis indicated that the synthesis of Leu. lactis KCTC 13344 ${\beta}$-xylosidase (approximate size, 64 kDa) is induced by xylose. A maximum intracellular ${\beta}$-xylosidase activity of $7.1{\pm}0.3units/mL$ was obtained in a batch cultivation in an MRS medium containing 30 g/L xylose.

Distribution Pattern, Geochemical Composition, and Provenance of the Huksan Mud Belt Sediments in the Southeastern Yellow Sea (황해 남동부 흑산니질대 퇴적물의 분포, 지화학적 조성 및 퇴적물 기원지)

  • Ha, Hun Jun;Chun, Seung Soo;Chang, Tae Soo
    • Journal of the Korean earth science society
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    • v.34 no.4
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    • pp.289-302
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    • 2013
  • In order to determine the provenance of the Huksan Mud Belt sediments in the southeastern Yellow Sea, the major and rare earth elements of the same sediments were analyzed. The surface sediments were sampled from top of piston-cores and box-cores taken at 51 sites within the Huksan Mud Belt. With the mean grain size of $5-6{\phi}$, the sediments of the study area are mud-dominated. The spatial distribution patterns show that silt content is high in the northern Mud Belt, whereas clay content increases as it moves toward the southern Mud Belt. Interestingly, the geochemical compositions both of major and rare earth elements have resulted in differences of sediment provenance. Among the major elements, plots of Fe/Al vs. Mg/Al ratios, $Al_2O_3$ vs. MgO ratios, and $Al_2O_3$ vs. $K_2O$ reveal that the Huksan Mud Belt sediments are dominated by the Korean river-derived sediments. However, the characteristics of rare earth elements infer sediments originating from the Chinese rivers. This discrepancy between the above provenances is attributed to the different contributory factors in the content of chemical elements. Considering strong correlation between major elements with grain sizes, the contents of the major elements are thought to be influenced by the grain size. However, there is a weak correlation between rare earth elements and grain sizes. The behaviour of rare earth elements may be controlled by heavy minerals, rather than grain sizes. Further study requires to solve the discrepancy arose from the difference in applied chemical tracers.

Effect of syllable complexity on the visual span of Korean Hangul reading and its relation to reading abilities (한글 글자 유형이 시각 폭과 읽기 능력에 미치는 영향)

  • Choi, Youngon;Kim, Tae Hoon
    • Korean Journal of Cognitive Science
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    • v.27 no.2
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    • pp.325-353
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    • 2016
  • The visual span refers to the number of letters that can be accurately recognized without moving one's eyes. The size of the visual span is affected by sensory factors such as perimetric complexity, crowding, and mislocation of letters. Korean Hangul utilizes rather unique alphabetic-syllabary writing system, quite different from English and Chinese writing systems. Due to this combinatorial nature of the script, the visual span for Hangul characters can also be affected by the letter type (e.g., CV vs CVCC). The present study examined the effect of syllable complexity on the visual span for Hangul by comparing letter recognition accuracy across four letter type conditions (C only, CV, CVC, and CVCC). We also aimed to determine the meaningful letter type(s) that is associated with differences in reading abilities in Korean. Using a trigram presentation method, we found that overall recognition accuracy declined as syllable complexity increased. However, the visual span for CVC type was greater than that for CV type, suggesting that the effect is not necessarily linear, and that there might be other factors affecting the visual span for these types of letters. C and CV type showed fairly strong positive correlations with reading comprehension, suggesting that these might be the meaningful units for measuring visual span in relating to reading abilities.

Development of Slow-Release Compound Fertilizer Used Urea-Resin for Upland Crop I. Manufacture of Slow-Release Compound Fertilizer (요소수지(尿素樹脂)를 이용(利用)한 전작용(田作用) 완효성(緩效性) 복비(複肥) 개발(開發) I. 제조시험(製造試驗))

  • Seong, Ki-Seog;Kim, Bok-Jin;Shin, Jae-Sung
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.3
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    • pp.193-198
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    • 1990
  • In order to develop the slow-release compound fertilizer fur upland crops, trial products were made with ammonium sulfate, diammonium phosphate, and potassium chloride with filler of calcium sulfate, and hinder of additional material of urea resin Nine trial slow-release compound fertilizers were manufactured by varying urea/formaldehyde mole ratios(U/F mole ratio : 0.5, 1.0, and 1.5 ) and the proportions of urea resin (3.3. 6.7. and 10%) and the proportions of urea resin (3.3. 6.7 and 10%). The aimed percentage of $N-P_2O_5-K_2O$ in the trial product was 12.5-10.0-14.0%, which was appropriate for Chinese cabbage. The N dissolution of the trial products in water was slower with lower U/F ratio and in creased to the proportion of urea resin. The dissolved nitrogen in water after 24hrs ranged from 69 to 83 percent of total nitrogen in the trial products.

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Effect of Highly Water Absorbing Polymer(K-sorb) on Soil Water Retention (토양의 수분보유(水分保有)에 미치는 초흡수성 고분자중합체(高分子重合體)(K-sorb)의 효과)

  • Yoo, Sun-Ho;Kwun, Sun-Kuk;Ro, Hee-Myeong
    • Korean Journal of Soil Science and Fertilizer
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    • v.23 no.3
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    • pp.173-179
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    • 1990
  • The effect of a highly water absorbing polyacrylate polymer, commonly called K-sorb, at rates of 0.0, 0.05, 0.2, and 0.5% by weight on the water retention properties of three soils, and the longevities of these treatment effects were evaluated. Water retentions were measured for all the treatments by use of a pressure-plate extractor in the laboratory. Available water and three-phase distributions at moisture tensions of 0.01, 0.3, and 15b were calculated from water retentivity data. A randomized block experiment of Chinese cabbages was conducted to examine the effects and the longevities of the treatments(0.0, 0.05, 0.1, and 0.2%) on water retention of Jungdong sandy loam soil in the field. Water retentions for a loamy sand, sandy loam, and loam soil, treated with 0.2 and 0.5% K-sorb, were increased. K-sorb treatments were more effective in sandy soil than in loamy soils. Water contents for the 0.5% treatment were markedly greater than those for the 0.2% treatment at earth moisture tension. K-sorb only at a rate of 0.5% remained effective in water retention of each soil through repeated drying and wetting for 12 months. Duncan's multiple range showed 0.2% treatment was effective(at the level of 0.05) after 2 months but not after 10 months under field condition.

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Effect of Interillage and Muddying by Free-Ranging Ducks on pH and Dissolved Oxygen of Stagnant Water, and Soil Physicochemical Properties in Paddy field Soil (논 방사오리에 의한 중경탁수가 관개수의 pH, 용존산소농도 및 토양 이화학적 성질에 미치는 영향)

  • 고병대
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.3
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    • pp.262-266
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    • 2003
  • The present study was carried out to investigate the relationships among intertillage and muddying by free-ranging ducks (Chinese native ducks) and pH, dissolved oxygen of irrigation water, and physicochemical properties of soil. Two paddy fields comprised of the muddying (muddy water without physical stimulation to a rice plant by the ducks) and the control plots (no ducks) were used for this study. As a muddying plot, 50 hills were randomly selected and each hill was fenced with a wire net in order to prevent from stimulating physically by the ducks. The variation in irrigation pH was smaller in the muddying plot than the control plot, and the pH was around 7.4. Dissolved oxygen was significantly higher (P<0.01) in the muddying plot than the control plot during the first half period after the ducks were introduced. Then it decreased rapidly as the rice plants grew in all plots. The contents of the suspended soil and turbidity in irrigation water were not changed over the free-ranging period, but $\textrm{NH}_3\textrm{-N}$ and $\textrm{P}_2\textrm{O}_5$ were decreased by intertillage and muddying by the ducks. There were no differences in both the soil physics and chemistry between the muddying plot and the control plot during and after finishing of the free-ranging period. These results indicate that intertillage and muddying by the ducks resulted in a small variation in stagnant water pH, and reduced the dissolved oxygen and $\textrm{P}_2\textrm{O}_5$, although the physiochemical properties of soil were not changed.

Utilization of Information from International Observation Trials for the Introduction of New Crops: An Introduction of Azuki Bean Varieties from China to Thailand

  • Xin, Chen;Volkaert, Hugo;Chatwachirawong, Prasert;Srinives, Peerasak
    • Journal of Crop Science and Biotechnology
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    • v.11 no.1
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    • pp.51-56
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    • 2008
  • Azuki bean has never been commercially grown in Thailand, due in part to a lack of suitable varieties. A core collection of 114 azuki bean accessions, originally from different parts of China(northern, central, southern) representing the germplasm of Chinese land races, were evaluated in the experimental field of the Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, China from June to October 2004. The same experiment was repeated at Kamphaeng Saen campus of Kasetsart University, Thailand from February to May 2005. Yield, yield components, and agronomic traits were recorded in all accessions in order to identify certain genotypes for further investigation. The statistical parameters that were used as indicators of phenotypic variation were mean, coefficient of variability(CV), correlation coefficient(r), range, mean difference, and phenotypic clustering of the accessions. The results indicated that the azuki bean varieties planted in Kamphaeng Saen were shorter, earlier in growing duration, and lower in plant height, seed yield per plant, 100-seed weight, and pods per plant as compared to when they were grown in China. This discrepancy was caused largely by the combined effect of temperature, rainfall, and day length. The traits that were rather stable in both locations were branches per plant and seeds per pod. Azuki bean varieties from northern China showed higher response to the changing environments compared with those from central and southern China. Some agronomic traits showed high correlation coefficient between the environments in Thailand and China. The CV of agronomic traits in both locations were ranked in descending order as follows: seed yield per plant, pods per plant, branches per plant, plant height, 100-seed weight, seeds per pod, and growing duration. The CV of seeds per pod and branches per plant were almost the same in both locations. Yield per plant in China correlated well(r=0.75) with pods per plant, but not with the other traits. Based on their response to both environments, the azuki bean accessions can be broadly divided into four groups, viz. northern 1, northern 2, central, and southern. This implied that there was more diversity, but probably less stability among the accessions originating from northern China.

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Use of Pythium myriotylum MD2 to Control Weeds in Rice Paddy Fields (물달개비 병원균인 Pythium myriotylum MD2를 이용한 논잡초 방제)

  • Choi, Gyung-Ja;Hwang, In-Taek;Choi, Yong-Ho;Lee, Byung-Hoi;Kim, Heung-Tae;Kim, Jin-Cheol;Lee, Seon-Woo;Kim, Jin-Won;Cho, Kwang-Yun
    • The Korean Journal of Pesticide Science
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    • v.5 no.4
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    • pp.33-39
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    • 2001
  • An isolate of the indigenous fungus Pythium myriotylum was isolated from Monochoria vaginalis in Yusung, Korea in year 2000 and evaluated potential as a biocontrol agent in laboratory and greenhouse. P. myriotylum MD2 grew in a wide range of temperature regimes and the optimal growth temperature was $35^{\circ}C$. The fungus was highly pathogenic to Monochoria vaginalis at 30 to $35^{\circ}C$. Several weeds such as Rotala, indica, Lindernia procumbens, Ludwigia prostrata, Cyperus difformis, Scirpus juncoides, Aneilema keisak were also susceptible to the fungus, but Echinochloa crus-galli was not. The fungus affected the growth of rice seed germinated, but not to rice seedlings of 1- to 3-leaf stage. A total of 12 rice cultivars (3- to 4-leaf stage) tested showed no disease symptoms when inoculated with the fungus. Eleven crops, including Chinese cabbage, corn, soybean except wheat were immune to the infection of the fungi. These data suggest that P. myriotylum MD2 has a potential as a mycoherbicide to control weeds in paddy fields.

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Application of Neutral Red Uptake Assay Using EPC Cells as an Alternative to the Fish Acute Toxicity Test for Pesticide (어류급성독성시험 대체법으로서 잉어표피세포를 이용한 Neutral Red Uptake 분석법 적용)

  • Seo, Ji-Hyun;Park, June-Woo;Lee, Sung-Kyu;Kim, Woo-Keun
    • The Korean Journal of Pesticide Science
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    • v.18 no.1
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    • pp.8-13
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    • 2014
  • This study evaluated in vitro cytotoxicity of 5 pesticides, including 2 herbicides, 2 germicides, and an insecticide, as an alternative to the fish acute toxicity test. The in vitro cytotoxicity was tested using a neutral red uptake (NRU) assay with epithelioma papulosum cyprini (EPC) cells that originated from the epidermal tissue of Cyprinus carpio (common carp). An in vivo fish acute toxicity test was conducted according to OECD Test Guideline No. 203 using Aphyocypris chinensis (Chinese bleak), Oryzias latipes (Japanese medaka), and C. carpio. The results showed that the sensitivity of the cell viability assay for the pesticides was similar to the fish acute test in ranking order despite having approximately 10 times less absolute sensitivity. The $r^2$ correlation values were calculated as 0.38 (p = 0.26), 0.76 (p = 0.05) and 0.90 (p = 0.01) for A. chinensis, O. latipes, and C. carpio, respectively. These results suggested that the potential of EPC cell viability assay as an alternative to the fish acute toxicity test due to their good correlation and NRU assay is expected to serve as a useful tool for predicting acute fish lethality for pesticides if further studies with a large set of pesticides are conducted.

Practical approaches to becoming the logistics hub of Northeast Asia (동북아 물류중심국가 추진전략에 관한 연구)

  • Oh, Moon-Kap
    • Journal of Distribution Science
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    • v.11 no.6
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    • pp.31-40
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    • 2013
  • Purpose - The Northeast Asian Logistic Hub strategy was established to create a national competitive advantage in northeast Asia. Countries in this region are competing fiercely to become the central base distribution port as the volume of container shipping continues to increase due to the northeast Asian (especially Chinese) economic growth. The primary method by which shippers are improving their customer service and distribution is enhancing profits by minimizing call ports on the key route through strategic affiliations and the use of large vessels. Each nation is planning large-scale investments in the construction of sea ports that can accommodate large vessels. This paper proposes ways by which the logistical strategies of domestic corporations can keep pace with changes in government policy concerning the Northeast Asian Business Hub policy. It examines the logistics system in the Northeast Asian region, analyzes the government's Northeast Asian Business Hub policy, and suggests logistical strategies for domestic corporations through an analysis based on a questionnaire designed to grasp domestic firms' needs and goals. Research design, data and methodology - The purpose of this study is to determine how shipping companies establish partnerships with third-party logistics providers and draws out the implications of the results. The survey methods used were personal interviews and questionnaires distributed to a sample population through e-mail, fax, mail, and telephone. A total of 600 questionnaires were distributed, of which 165 were returned. Among these, ten were excluded due to insufficient content; ultimately, 155 were used for the sample. The statistical data collection process was analyzed through data coating and a statistical package program. Results - This study argues that greater flexibility in policies, administration, and systems will be needed to significantly improve established business practices. In this dissertation, we primarily identify that in order to become a center of northeast Asian logistics, Korea must adopt a new paradigm and abandon the existing systems that are based on the economic and social systems that have stemmed from bureaucracy, inflexibility, chauvinism, and equalitarianism. Flexible policies, administration, and systems will be necessary to improve business practices. Domestic corporations must establish a strategic logistics hub and related network while simultaneously pursuing value-added logistics businesses by increasing their manpower and building a logistics information system. This will strengthen their competitive edge and lead to system improvements. Conclusions - Domestic corporations must adopt a new paradigm and use more reasonable business laws, systems, and policies that are based on market-driven flexibility and transparency. Moreover, social norms and regulations should be established to help ensure political and social security. Korea must also develop a culture of tolerance for foreign companies. Finally, the paradigm defining the policy governing the development of the capital city and its satellite cities in this context must be changed.