• 한국환경성돌연변이발암원학회지
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• 제17권1호
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• pp.17-22
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• 1997

We observed the frequency of chromosome aberrations induced by UVB irradiations, and the suppressing effect of tannic acid on chromosome aberrations induced by UVB irradiations in CHL cells, which is a phenolic compound, a hydrolysate of tannin and a components of green tea. UVB doses used for the frequency of chromosome aberrations were from 0.2 to 1.6 KJ/m$^2$ and tannic acid concentrations were from 1.16 $\mu$g/ml to 37.50 $\mu$g/ml. For the observation of suppressing effect of tannic acid on UVB-induced chromosome aberrations, UVB dose was 1.6 KJ/m$^2$ and tannic acid concentrations were 1.0, 2.0, 4.0 $\mu$g/ml. In our study, tannic acid was treated for 24 hours in CHL, cells after UVB irradiation without S9 mix or for 6 hours with S9 mix. From this study, we obtained the following results : (1) The frequency of chromosome aberrations UVB induced were dose-dependently increased. (2) The tannic acid did not induce chromosome aberrations in cultured Chinese hamster cells. (3) UVB-induced chromosome aberrations were suppressed by tannic acid at every concentration from 1.0 $\mu$g/ml to 4.0 $\mu$g/ml with or without metabolic activation. These results suggest that the tannic acid acts as an inhibitor to UVB-induced clastogenicity of the cultured cell.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.157.3-158
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• 2003

The antioxidant and anticancer properties of a medicinal plant, Betula platyphylla var. japonica were investigated. The total methanol extract of B. platyphylla var. japonica had protective effects against hydrogen peroxide ($H_2O_2$) in the Chinese hamster lung fibroblast (V79-4) cell line and induced apoptotic cell death in human promyelocytic leukemia (HL-60) cells, a cancer cell line. B. platyphylla var. japonica extract significantly increased cell viability against $H_2O_2$. The extract also showed high 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity ($IC_50$ 2.4 mg/ml) and lipid peroxidation inhibitory activity ($IC_50$ below 4.0 mg/ml). (omitted)

• Archives of Pharmacal Research
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• 제21권4호
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• pp.391-397
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• 1998

The toxicity evaluation of oriental herbal drugs is of great concern at present. Bojungchisup-tang (BCST, in Korean), a decocted medicine of oriental herbal mixture, is now well used in clinic at oriental hospitals for the treatment of edema of several diseases in practice. However, the toxicity of the oriental herbal decocted medicines such as genetic toxicity is not well defined until now. In this respect, to clarify the genetic toxicity of BCST, in vitro chromosome aberration assay with Chinese hamster lung (CHL) fibroblasts and in vivo supravital micronucleus assay with mouse peripheral reticulocytes were performed in this study. In the chromosome aberration assay, we used 5,000 $\mu\textrm{g}$/ml BCST as maximum concentration because no remarkable cytotoxicity in CHL cells was observed both in the presence and absence of S-9 metabolic activation system. No statistical significant differences of chromosome aberrations were observed in CHL cells treated with 5,000, 2,500 and 1,250 $\mu\textrm{g}$/ml BCST for 6 hour both in the presence and absence of S-9 metabolic activation. However, very weak positive result (6.5-8.0% aberration) of BCST was obtained in the absence of S-9 metabolic activation system at 5,000 $\mu\textrm{g}$/ml BCST when treated for 24 hour, i.e. 1.5 normal cell cycle time. And also, in vivo clastogenicity of BCST was studied by acridine orange-supravital staining micronucleus assay using mouse peripheral reticulocytes. We used 2,000 mg/kg as the highest oral dose in this micronucleus assay because no acute oral toxicity of BCST was observed in mice. The optimum induction time of micronucleated reticulocytes (MNRETS) was determined as 36 hours after oral administration of 2,000 mg/kg BCST. No significant differences of MNRETs between control and BCST treatment groups were observed in vivo micronucieus assay. From these results, BCST revealed very weak positive result in chromosome aberration assay in vitro with CHL cells and no clastogenicity in micronucieus assay in vivo.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 1996년도 제19회정기학술대회(The 19th Symposium of the Korean Society of Environmental Toxicology)
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• pp.63-63
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• 1996

We performed chromosomal aberration assay in Chinese Hamster Lung (CHL) cells in vitro to evaluate theclastogenicity of 11 synthetic chemicals which were listed in Toxicity Evaluation Program of Ministry of Environment of Republic of Korea in 1996. All of the chemicals were carried out MTT assay to determine the 50% cell growth inhibition concentration. All compounds were tested with and without metabolic activation system. Benzoyl chloride revealed positive result at $43\;\mu\textrm{g}/m{\ell}$ in the presence of metabolic activation system, and at 30.8, 61.5 and $123\;\mu\textrm{g}/m{\ell}$ in the absence of metabolic activation system. And p-phenoxy ethanol was observed as positive with the metabolic activation system, but negative without metabolic activation system. Especially 2-propyn-l-ol showed high frequency of pulverization and showed critical difference of cytotoxicity between with and without S9 mixture. Pulverizatiuon is not included in the frequency of structural aberration in our criteria. Dicyclopentadiene, methacrylic acid, aa-dimethylbenzyl hydroperoxide, benzylbutyl phthalate, and p-chlorophenal were revealed negative results.esults.

• 한국식품과학회지
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• 제29권4호
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• pp.804-807
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• 1997

제일제당주식회사에서는 미생물발효법을 이용하여 palatinose를 대량생산하게 되었다. Palatinose 산물의 안전성을 확인하기 위하여 1) Salmonella typhimurium을 이용한 미생물복귀돌연변이시험, 2) Chinese Hamster Lung (CHL) 세포를 이용한 in vitro 염색체이상시험을 실시하였다. Palatinose 및 palatinose syrup은 미생물복귀돌연변이 시험에서 10 mg/plate의 용량까지 복귀돌연변이를 유발하지 않았으며, CHL 세포에서도 5 mg/mL 농도에서 염색체이상을 유발하지 않았다. 이 결과는 palatinose 산물들이 위의 in vitro 변이원성시험계에서 돌연변이원성을 나타내지 않음을 보여준다.

• 한국식품저장유통학회지
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• 제16권5호
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• pp.782-788
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• 2009

감마선 조사 계란의 유전독성학적 안전성을 평가하기 위하여Escherchia coli PQ37 균주를 이용한 SOS chromotest, Chinese hamster lung cell을 이용한 염색체 이상 시험의 in vivo 시험과in vivo 시험으로 마우스를 이용한 소핵시험을 실시하였다. 대사활성계 적용 및 미적용의 in vitro의 모든 시험에서 음성대조군과 통계학적으로 유의적인 차이는 없었다. 또한in vivo 시험에서도 시험물질을 투여한 모든 투여군에서 음성대조군에 비해 통계학적으로 유의적인 독성을 나타내지 않았다. 따라서 감마선 조사된 난백 및 난황은 본 시험조건하에서 직접변이원이나 간접변이원으로 작용하지 않는 것으로 사료된다.

• 대한임상검사과학회지
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• 제46권2호
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• pp.75-78
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• 2014

Green tea and tea polyphenols have been studied extensively as cancer chemopreventive agents in recent years. Epigallocatechin-3-gallate (EGCG) is widely recognized as a powerful antioxidant and a free radical scavenger. The purpose of this study was to evaluate the protective effects of green tea catechins (GTC) on the Bleomycin- and Cyclophosphamide-induced cytotoxicity. Cell viability was measured by MTT assay. In the protective effect of GTC, the cell viability was significantly increased by the treatment of GTC. Furthermore, GTC showed the higher protective effect than EGCG and vitamin E. These results suggest that GTC has the protective effect which is related to the prevention of cancer. Our studies show that the continuous presence of EGCG can reduce radical-induced DNA damage in Chinese hamster lung fibroblast cells (CHL cells).

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.179.1-179.1
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• 2003

CKD-712, named S-YS49 is a chiral compound derived from higenamine (one component of Aconite spp.) derivatives. To compare the cytotoxicity of CKD-712 between in the absence and in the presence of S9 metabolic activation system, we performed try pan blue dye exclusion assay in Chinese hamster lung (CHL) cell. In CHL cells, the cytotoxicity ($IC_{50}$) of CKD-712 was 92.9 ${\m}g$/$m\ell$ and 186.1 ${\m}g$/$m\ell$ in the absence and presence of S9 metabolic activation, respectively. (omitted)

• 한국식품조리과학회지
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• 제22권4호통권94호
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• pp.428-437
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• 2006

본 연구에서는 천연 허브류의 항산화성 및 세포주를 이용한 세포 독성, 산화적 스트레스에 대한 보호 효과, 항산화 효소 활성 등을 살펴보았다. Chinese Hamster lung fibroblasts인 V79-4에서는 green tea 추출물, 고농도의 lemon 추출물에서 세포독성을 보였다. H$_2$O$_2$로 유발한 산화적 스트레스에 대해 허브 추출물의 V79-4 세포 보호효과는 모든 추출물이 농도 의존적으로 효과를 갖지는 않으나chamomile, fennel, dandelion을 제외한 대부분의 고농도추출물에서 대조군에 비해 세포 보호 효과를 가지고 있다고 보인다. 항산화 활성 및 세포 독성 실험을 토대로 활성이 높은 5가지 허브 추출물의 V79-4 세포를 이용한 항산화 효소 활성에서 lemon vervena, chamomile을 처리하면 superoxide dismutase, glutathione peroxidase 활성이 대조군보다 증가하는 결과를 보였다. 이러한 다양한 허브 추출물의 항산화 활성 검색을 토대로 허브류의 식품 소재로서의 가능성을 평가할 수 있을 것이다.

• 한국환경성돌연변이발암원학회지
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• 제22권4호
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• pp.319-323
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• 2002

In order to investigate the safety of Aralia elata extract causing the reduction in the blood glucose level and oxidative stress in diabetes animals, these genotoxicity studies in bacterial and mammalian cell assay system such as Ames bacterial reverse mutation test and chromosomal aberration assay were performed. As results, in Ames bacterial reversion assay the extract in the range of 5,000-625 ug/plate did not induce mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 strains with and without metabolic activation of S-9 mixture. For chromosomal aberration assay, $IC_{50}$ (50% inhibition concentration of cell growth) of the extract were determined; 792 $\mu\textrm{g}$/$m\ell$ without and 524 $\mu\textrm{g}$/$m\ell$ with S-9 mixture in Chinese hamster lung (CHL) fibroblast cell culture. Any significant chromosomal aberration was not observed in CHL cells treated with the extract at the concentrations of 792, 396 and 198 $\mu\textrm{g}$/$m\ell$ or 524, 262 and 131 $\mu\textrm{g}$/$m\ell$ in the absence or presence of S-9 metabolic activation, respectively. From these results, Aralia elata extract did not induce any harmful effects on the gene in bacteria and mammalian cell system used in these experiments.