Korean journal of food and cookery science (한국식품조리과학회지)

Korean Society of Food and Cookery Science (한국식품조리과학회)
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Screening of Natural Herb Methanol Extracts for Antioxidant Activity in V79-4 cells

천연 허브 메탄올 추출물의 V79-4 세포에서 항산화 활성 검색

  • Chang, Jeong-Hwa (Department of Food and Nutrition, College of Human Ecology, Seoul National University) ;
  • Yoo, Kyung-Mi (Research Institute of Human Ecology, Seoul National University) ;
  • Hwang, In-Kyeong (Department of Food and Nutrition, College of Human Ecology, Seoul National University)
  • 장정화 (서울대학교 식품영양학과) ;
  • 유경미 (서울대학교 생활과학연구소) ;
  • 황인경 (서울대학교 식품영양학과)
  • Published : 2006.08.31
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Abstract

To investigate the worth of herbs as functional food ingredients, the antioxidant activity of 15 kinds of herb mathanol extracts was evaluated. Green tea, chamomile, dandelion, and lemon vervena extracts, with IC$_{50}$ values of 1.45 g/100mL, 1.49 g/100mL, 1.50 g/100mL and 1.55 g/100mL, respectively, had significantly higher superoxide radical scavenging activity than any other herb extracts. Green tea and lemon vervena extracts, which had high radical scavenging activity, showed inhibition of cell proliferation in Chinese hamster lung fibroblasts (V79-4 cells). Most herb extracts, except for chamomile, fennel and dandelion enhanced cell viability against H$_2$O$_2$-induced oxidative damage in V79-4 cells. At a dose of 1600 ${\mu}$g/mL, lemon vervena, green tea, hawthorn and rosemary extracts showed a cell viability of more than 50% which was significantly higher than that of the control culture treated with only H$_2$O$_2$ Thus, the results suggest that some herb extracts exhibited a V79-4 cell protective effect. The investigation of the cellular antioxidant enzymes activities of the five selected herb extracts revealed that extracts of lemon vervena and chamomile dose-dependently increased superoxide dismutase and glutathione peroxidase activity but that this increase was not significant. In conclusion, some natural herb extracts exhibited high antioxidant activity.

본 연구에서는 천연 허브류의 항산화성 및 세포주를 이용한 세포 독성, 산화적 스트레스에 대한 보호 효과, 항산화 효소 활성 등을 살펴보았다. Chinese Hamster lung fibroblasts인 V79-4에서는 green tea 추출물, 고농도의 lemon 추출물에서 세포독성을 보였다. H$_2$O$_2$로 유발한 산화적 스트레스에 대해 허브 추출물의 V79-4 세포 보호효과는 모든 추출물이 농도 의존적으로 효과를 갖지는 않으나chamomile, fennel, dandelion을 제외한 대부분의 고농도추출물에서 대조군에 비해 세포 보호 효과를 가지고 있다고 보인다. 항산화 활성 및 세포 독성 실험을 토대로 활성이 높은 5가지 허브 추출물의 V79-4 세포를 이용한 항산화 효소 활성에서 lemon vervena, chamomile을 처리하면 superoxide dismutase, glutathione peroxidase 활성이 대조군보다 증가하는 결과를 보였다. 이러한 다양한 허브 추출물의 항산화 활성 검색을 토대로 허브류의 식품 소재로서의 가능성을 평가할 수 있을 것이다.

Keywords

References

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