• Bulletin of the Korean Chemical Society
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• v.31 no.12
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• pp.3703-3706
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• 2010

Biomolecules specific to explosives can be exploited as chemical sensors. Peptides specific to immobilized dinitrotoluene (DNT) were identified using a phage display library. A derivative of DNT that contained an extended amine group, 4-(2,4-dinitrophenyl)butan-1-amine, was synthesized and immobilized using a self-assembled monolayer surface on gold. Filamentous M13 phages displaying random sequences of 12-mer peptides specific to the immobilized DNT-derivate were isolated from the M13 phage library by biopanning. A common peptide sequence was identified from the isolated phages and the synthesized peptides showed selective binding to DNT. When the peptide was immobilized on a quartz crystal microbalance (QCM) chip, it showed a binding signal to DNT, while toluene barely showed significant binding to the QCM chip. These results demonstrate that peptides screened by biopanning against immobilized DNT can be useful for quick and accurate detection of DNT.

• The Korean Journal of Pesticide Science
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• v.8 no.1
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• pp.8-15
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• 2004

A synthesis of new $\beta$-ketoacetoanilide chloride derivatives and anti fungal activity of these compounds library against 6 typical plant pathogens were described. Reaction of ketene dimer with chlorine followed by treatment of aniline derivatives gave 89 kinds of the corresponding $\beta$-ketoacetoanilide chlorides through combinatorial synthetic technology using Carousel Reaction Stations. Evaluation of antifungal activity (in vivo) of this chemical library against rice blast, rice sheath blight, tomato aray mold, tomato late blight, wheat leaf rust and barley powdery mildew was carried out. In general, $\beta$-ketoacetoanilide chlorides which present a substituent at 4 in phenyl group(para) of the compounds showed selective control activity against tomato late blight caused by Phytophthora infestans.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1655-1660
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• 2007

A metagenome is a unique resource to search for novel microbial enzymes from the unculturable microorganisms in soil. A forest soil metagenomic library using a fosmid and soil microbial DNA from Gwangneung forest, Korea, was constructed in Escherichia coli and screened to select lipolytic genes. A total of seven unique lipolytic clones were selected by screening of the 31,000-member forest soil metagenome library based on tributyrin hydrolysis. The ORFs for lipolytic activity were subcloned in a high copy number plasmid by screening the secondary shortgun libraries from the seven clones. Since the lipolytic enzymes were well secreted in E. coli into the culture broth, the lipolytic activity of the subclones was confirmed by the hydrolysis of p-nitrophenyl butyrate using culture supernatant. Deduced amino acid sequence analysis of the identified ORFs for lipolytic activity revealed that 4 genes encode hormone-sensitive lipase (HSL) in lipase family IV. Phylogenetic analysis indicated that 4 proteins were clustered with HSL in the database and other metagenomic HSLs. The other 2 genes and 1 gene encode non-heme peroxidase-like enzymes of lipase family V and a GDSL family esterase/lipase in family II, respectively. The gene for the GDSL enzyme is the first description of the enzyme from metagenomic screening.

• Proceedings of the Korea Contents Association Conference
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• 2007.11a
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• pp.487-489
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• 2007

In this paper chemical process plant information is addressed to be standardized. The chemical process plant information has complexities in the context of its various types such as functions, shapes, connectivity etc. as well as its usages such as design, construction, operation, maintenance and so on. It was well known that the standard should be made by its users themselves to be used widely. The chemical process industry oriented ISO 15926 RDL (Systems and integration of life-cycle data for oil and gas production facilities : Reference Data Library) can provide the methodology for exchanging and sharing of plant equipment information, which will be developed through integrating existing standards such as ISO 10303 AP221, AP231, AP227. The PCA (Posc Caesar Association) which is located in Oslo, Norway, has the initiative in developing ISO 15926. Korea is a member of PCA and takes part in proposing and reviewing the new standard. The trends and prospects for the standard of chemical process plant information are addressed.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3923-3926
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• 2013

• Bulletin of the Korean Chemical Society
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• v.35 no.7
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• pp.1989-1995
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• 2014

The small library of new enantiomerically pure (S,S)-diamino alcohols 1 and their hydroxyldiamide precursors 2 were conveniently synthesized on a gram scale from inexpensive and commercially chiral pool amino acids. The catalytic and induced asymmetric effects of the chiral ligands 1 in the asymmetric allylic oxidation of cycloolefins were investigated.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.24 no.3
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• pp.205-211
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• 2012

Unlike the previous approaches, we analyzed for economic efficiency of renewed heating and cooling systems as the characteristics of space. The purpose of this study is to analysis the energy consumption and economical efficiency of absorption chillers and EHP systems in renovated library. It is important that equipment selection should be considering energy cost as well as space program. In recently, many EHP systems were installed in the building for reducing the energy cost and for seeking the convenience of individual control. In contrast, though absorption chillers have the disadvantage of a central control, absorption chillers are appropriate for the conditions of the library that needs simultaneous operation. The results by payback period method, show that selection of heating and cooling systems should be consider for space program and the characteristics of space.

• Bulletin of the Korean Chemical Society
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• v.33 no.5
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• pp.1597-1602
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• 2012

In this study, we determined the 3D-structure of Arabidopsis thaliana KAPAS by homology modeling. We then investigated the binding mode of compounds obtained from in-house library using computational docking methods. From the flexible docking study, we achieved high dock scores for the active compounds denoted in this study as compound $\mathbf{3}$ and compound $\mathbf{4}$. Thus, we highlight the flexibility of specific residues, Lys 312 and Phe 172, when used in active sites.

• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.777-782
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• 2007

A novel library of amino-substituted 2-pyridones has been constructed through a two-step sequence of microwave-promoted Buchwald-Hartwig amination of 2-benzyloxy halopyridines followed by debenzylation. Microwave-promoted amination of 3- or 4-halopyridine in the presence of a suitable palladium catalyst and ligand system provided amino-substituted 2-benzyloxypyridines in excellent yields. Then, debenzylation of 2- benzyloxypyridines afforded the corresponding 2-pyridones with high efficiency.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.191-202
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• 2004

한국화합물은행(KCB)에서 보유중인 12만 개의 화합물을 주요 골격에 따라 분류하고, 4 가지 protease 작용점에 대한 활성도와 골격사이의 관계를 조사하였다. 화합물들은 합성기관의 합성 목적과 주요 고리골격 등을 고려하여 분류되었으며, 이를 이용하여 scaffold 분류를 위한 분류 계통도를 작성하였다. 화합물들은 이 계통도에 따라 7 가지의 race, 168 tribe, 493 parent, 439 child, 325 grandchild 등 1,087개의 scaffold로 분류되었으며, 각 race 및 scaffold 별 골격의 개수는 고르게 분포되었다. 골격별 분류 시스템을 이용하여 4 가지의 protease에 대한 활성도와 골격 간의 상관관계를 조사한 결과, Protease C에 대하여 몇 가지 골격이 활성이 뛰어남을 보였다.