• Title/Summary/Keyword: Chemical leaves

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Isolation and Identification of Inhibitory Compounds from Morus alba cv. Kuksang on α-amylase and α-glucosidase (국상(Kuksang) 뽕잎(Morus alba L.)으로부터 α-amylase와 α-glucosidase 저해 물질 분리 및 동정)

  • Choi, Moo-Young;Cho, Young-Je
    • Journal of Life Science
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    • v.25 no.8
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    • pp.870-879
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    • 2015
  • The objective of this research was to evaluate the inhibitory activities of phenolic compounds isolated from mulberry (Morus alba) leaves of 109 types against α-amylase and α-glucosidase. The inhibitory activity of the water extracts from Morus alba cv. Kuksang against α-amylase and α-glucosidase were determined as 93.8% and 48.7% respectively. The total phenolic content of extracts from Morus alba cv. Kuksang was 9.7±0.2 mg/g soluble in water and 14.3±0.2 mg/g soluble in ethanol. The inhibitory activity of the water extracts from Morus alba cv. Kuksang at 200 μg/ml phenolics concentration against α-amylase and α-glucosidase were determined as 100% and 82.6% respectively. The purification of inhibitory compounds was carried out by Sephadex LH-20 and MCI-gel CHP-20 column chromatography using a gradient elution procedure by nomal phase type (EtOH→distilled water) and reverse phase type (distilled water→MeOH). The quercetin was confirmed to be the chemical structure of the inhibitory compound against α-amylase and α-glucosidase by spectroscopic analysis of FAB-MS, NMR and IR spectrum.

Plant Species Selection Program for Ecological Restoration of Coastal Reclaimed Land -Focused on the Dumped Soil Area at Inchon Airport- (해안간척지의 생태적 환경복원을 위한 식물 선정에 관한 연구 -인천공항 사토장지역을 중심으로-)

  • Bae, Young-Hun;Lee, Dong-Kun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.4 no.3
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    • pp.66-74
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    • 2001
  • The main purpose of this thesis is to select the appropriate plants for the ecological restoration of coastal land. The soil and vegetation environment survey of the beach where the halophytes live now was executed for the sake. Firstly, the soil survey was done by the physical and chemical survey of beach soil and dumped soil. The soil is mostly sandy loam and sandy clay loam which has silty much. The beach soil where the salt plants live has more organic matters in the content than dumped soil because of the influx of the dead halophytes, a pioneer plant to this area. Secondly the vegetation survey was executed by the halophytes survey in the beach area in order to select the appropriate plants for ecological restoration. The shows two different areas in the vegetation divided by the salt density of soil. Artemisia fukudo, Juncus haenkei, Carex pumila, Silene armeia, Polyponum aviculare etc live in the less salt density area and it doesnt show collected. Salicornia herbacea, Suaeda asparagoides, Aster tripolium, Limonium tetragonum, Suaeda maritima, Scirus fluvitilis, Phragmtes communis, Suaeda japonica, Zoysia sinica etc halophytes live in the more salt density area and there are a few advent plant but many collected. So the salt density of soil limits the vegetation. The selection of appropriate plants for the ecological restoration programs was designed with 3 different standards considering the salt density of soil in the dumped soil area. The plan selection guidelines of thick salt density due to the salt density of soil, and economical efficiency and maintenance because the area is large. The guidelines of middle salt density area were made considering economical efficiency and maintenance because there are salt as well, and it is effective for the scenery if they have normal ecology but their leaves have good color and the flowers are beautiful. However I suggest beautiful flowery plants for the neutral plan area because this area is the entry point of the airport so the view is very important.

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Nutritive Evaluation of Some Browse Tree Legume Foliages Native to Semi-arid Areas in Western Tanzania

  • Rubanza, C.D.K.;Shem, M.N.;Otsyina, R.;Ichinohe, T.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.10
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    • pp.1429-1437
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    • 2003
  • Browse tree legume leaves from Acacia spp (A. nilotica, A. tortilis, A. polyacantha), Dichrostachys sp, Flagea villosa, Piliostigma thonningii, Harrisonia sp were evaluated for nutritive potential (chemical compositions and degradability characteristics) compared to Gliricidia sepium. Effect of tannins anti-nutritive activity on digestibility was also assessed by polyethylene glycol (PEG) tannin bioassay. Crude protein (CP), ash, neutral detergent fiber (NDF), acid detergent fiber (ADF) and acid detergent lignin (ADL) differed (p<0.05) between legume foliages. Mean CP, ash, NDF, ADF and ADL for fodder species tested were 158, 92, 385, 145, and 100 g/kg DM, respectively. CP ranged from 115 (P. thonningii) to 205 g/kg DM (G. sepium). Acacia spp had moderate CP values (g/kg DM) of 144 (A. nilotica), to high CP in A. tortilis (188) and A. polyacantha (194) comparable to G. sepium. The forages had relatively lower fiber compositions. A. nilotica had (p<0.05) lowest NDF, ADF and ADL (182, 68 and 44) compared to P. thonningii (619, 196 and 130) g/kg DM, respectively. Except G. sepium, all fodder species had detectable high phenolic and tannin contents greater than 5% DM, an upper beneficial level in animal feeding and nutrition. Mean total phenolics (TP), total tannins (TT) and condensed tannins (CT) (or proanthocyanidins) for fodder species tested were 139, 113 and 43 mg/g DM, respectively. F. villosa had (p<0.05) lowest TP and TT of 65 and 56 mg/g DM, respectively, compared to A. nilotica (237 and 236 mg/g DM, respectively). The CT varied (p<0.05) from 6 (F. villosa) to 74 mg/g DM (Dichrostachys sp). In vitro organic matter (OM) degradability (OMD) differed (p<0.05) between fodder species. G. sepium had (p<0.05) high degradability potential compared to A. polyacantha that had (p<0.05) the lowest OMD values. Forage degradability ranked: G. sepium>A. nilotica>P. thonningi>F. villosa>Dichrostachys sp>A. tortilis>A. polyacantha. Addition of PEG resulted to (p<0.05) improvement in in vitro OM digestibility (IVD). Increase in IVD was mainly due to binding action of PEG on tannins; and represents potential nutritive values previously depressed by tannins anti-nutritive activity. Browse fodder has potential as sources of ruminal nitrogen especially for ruminants consuming low quality roughages due to high protein, lower fiber compositions and high potential digestibility. However, utilization of browse supplements in ruminants is hampered by high phenolic and tannin contents. Deactivation of tannin anti-nutritive activity, possibly by feeding tanniniferous browse with other readily available nitrogen sources to dilute tannin anti-nutritive activity could improve utilization of browse fodder supplements. Further studies are needed to assess browse fodder palatability and intake, and their effect on growth performance in ruminants.

Adsorption of Pb(II) by Cherry (Prunus x yedoensis) Leaf-Derived Biochar (왕벚나무 잎으로 제조된 바이오차의 Pb(II) 흡착특성)

  • Lee, Myoung-Eun;Hwang, Kyu-Taek;Kim, Sun-Young;Chung, Jae-Woo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.26 no.2
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    • pp.33-41
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    • 2018
  • Large amounts of leaves from street trees fall onto the streets annually and need to be cleaned and treated. Cherry trees are common street trees in Korea. The adsorption characteristics of Pb(II) by cherry leaf (CL) and cherry leaf-derived biochar (CB) were studied through a series of batch experiments. CB was produced through the carbonization of CL at $800^{\circ}C$ for 90 min. Carbonization increased the C content and pH value, while decreased H and O contents. Well developed pore structure was observed at the surface of CB. The pseudo-second order model better described the kinetics of Pb(II) adsorption onto CL and CB, indicating that the rate-limiting step of the heavy metal sorption is chemical sorption. Fast adsorption rates and high adsorption capacities were obtained by the carbonization from CL to CB. Langmuir models better adequately described the Pb(II) adsorption onto CL and CB. Maximum adsorption capacities of Pb(II) expressed by Langmuir constant, $Q^0$ were 37.31 mg/g and 94.34 mg/g, when CL and CB were used as adsorbents, respectively.

Volatile Aromatic Components of Ginger(Zingiber officinalis Roscoe) Rhizomes and Japanese Spice Bush(Lindera obtusiloba BL) (생강과 생강나무의 향기성분조성 비교)

  • 문형인;이재학
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.1
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    • pp.7-13
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    • 1997
  • The composition and chemical structures of same individual components of essential oils from ginger flavor plants were estimated by gas chromatography and gas chromatography-mass spetrometric analysis with the aid of NBS and Wiley library and RI indice searches. Through gas chromatography and gas chromatography /mass spetrometry analysis of 43, 41, 32 essential oil components from flowers, leaves and stems from Lindera obstusiloba., respectively were identified, among which sabinene, $\beta$-myrcene, ι-limonene, phelandrene, ${\gamma}$-selinene, $\alpha$-terpinene, 2, 4a, 5, 6, 7, 8, 9, 9a -octahydro benzocycloheptane, $\delta$-cadinene, ${\gamma}$-terpinene, (Z) -3-hexen-1-ol acetate, ${\gamma}$-elemene, l-boreneol, $\delta$-guaiene, ledene, cis-3-hexanal, elemol, $\alpha$-chamigrene, $\beta$-endesmol: 9-octadecanal, 1-(1, 5-diMe-4-hexenyl)-4-Me. benzene were estimated to be major components.

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Biological control of Gray Mold Rot of Perilla Caused by Botrytis cinerea II. Formulation of Antagonistic Bacteria and Its Control Effect (들깨 잿빛곰팡이병의 생물학적 방제 II. 미생물농약의 제조 및 그 방제효과)

  • Moon, Byung-Ju;Kim, Choul-Soung;Song, Ju-Hee;Kim, Ju-Hee;Lee, Jae-Pil;Park, Hyean-Cheal;Shin, Dong-Bum
    • Research in Plant Disease
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    • v.8 no.3
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    • pp.184-188
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    • 2002
  • An antagonistic bacteria, Bacillus licheniformis Nl strain which effectively inhibited mycelial growth of gray mold rot pathogen, Botrytis cinerea was isolated from the rhizosphere of perilla crop. Powder soy formulation by B. lichentfomis Nl strain as a biocontrol agent was developed far the first time and estimated its control effect on perilla leaves in this study. First of all, far the mass production of antifungal metabolites of B. lichentfomis Nl strain in flask liquid culture, the most effective carbon and nitrogen source were selected as glucose and tryp-tone, respectively, For the formulation, vegetative biomass of B. licheniformis Nl strain from 5-day-old liquid culture in nutrient broth added glucose and tryptone was mixed with soy flour, rice flour glucose, FeSo$_4$~7$H_2O$, and MnCl$_2$. 4$H_2O$, and dried and pulverized. In plastic house test, powder soy formulation effectually controlled gray mold rot as the control value of 93.1 %, was more effective than chemical fungicide, benomyl showing the control value of 86.1%. Thus, development of powder soy formulation of B. lichentfomis Nl will aid large-scale application of biological control in field trials.

Changes of Polyamine Content and Phytoavailability in Lactuca sativa cultivated in Cadmium and Arsenic Treated Soil (카드뮴과 비소처리가 상추의 polyamine함량 및 유효도에 미치는 영향)

  • Moon, Kwang-Hyun;Koh, Mun-Hwan;Kim, Won-Il;Jung, Goo-Bok;Kim, Kyung-Min
    • Korean Journal of Environmental Agriculture
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    • v.19 no.3
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    • pp.223-227
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    • 2000
  • Absorption of Cadmium(Cd) and Arsenic(As) by lettuce following to the change of polyamine content in lettuce were examined to find the effect of these elements on lettuce growth and on the basic resistance mechanism. As the contents of Cd and As were increased in soil, the contents of these elements were significantly increased in lettuce but lettuce growth was decreased. Sequential fractionation experiment of the Cd and As treated soil was shown that exchangeable, dilute acid extractable, and organically bound form, which were more mobile and phyto-available, of Cd were increased by 5.6, 42.9, and 56.7% during a growing season whereas 17.6, 25.0, and 24.1% were increased in case of As, respectively. Specially, the Cd content of leaves and roots in lettuce was positively correlated with exchangeable, dilute acid extractable, and residual form of Cd in the treated soil. However, the As content of roots in lettuce was positively correlated with all chemical forms of As in the treated soil. The contents of putrescine and cadaverine, a kind of polyamine, were also increased in lettuce as both elements were increased in soil.

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The Effect of Organic Fertilizer Granulated with Slurry of Glutamate Fermentation Residue on Corn and Chinese Cabbage (유기질(有機質) 특수비료(特殊肥料)의 시용(施用)이 옥수수 및 배추생육(生育)에 미치는 영향(影響))

  • Lim, Sun-Uk;Oh, Jae-Sup;Kim, Bok-Jin
    • Korean Journal of Soil Science and Fertilizer
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    • v.16 no.2
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    • pp.156-161
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    • 1983
  • In order to find out the effect and the optimum application amount of special organic fertilizer which is granuated with fermentation residue of glutamate two field experiments were conducted with corn and chinese cabbage. The results were summarized as follows: 1. The yields of corn and chinese cabbage were slightly increased with the application of special organic fertilizer. 2. There was fertilizer injury in chinese cabbage but not in corn when applied 300kg/10a after application of standard fertilizer levels for crops with general chemical fertilizers. 3. The absorption amount of total sugar in corn plant and content of vitamin E in chinese cabbage leaves after the harvest were increased with the application of special organic fertilizer. 4. In the soil where the special organic fertilizer was used, the organic matter content after experiment increased slightly but the tendency was not constant. 5. According to the increase of the special organic fertilizer application level, the soil pH after experiment decreased. This tendency was more clear at corn cultivated area.

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Taxonomical and Ecological Study on the Vegetation of the Seungbong Island (승봉도 식물상에 관한 분류 및 생태학적 연구)

  • Lee, Ho Joon;Seon Choel Choi;Young Hee Lee
    • Journal of environmental and Sanitary engineering
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    • v.5 no.2
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    • pp.105-116
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    • 1990
  • Seungbong Island is situated about 100km away Inchon Port in the western sea of the Korean Peninsula. Field investigations were conducted for two days each from June 30 through July 1, 1981 and from August 20 through 21, 1981. The results obtained are summarized as follows: Plants growing in this island consist of a total of 193 species (70 families, 151 genera, 170 species, 22 varieties and 1 forma). Pinus thunbergii was confirmed to be the dominant species. The natural degree observed in this island was high, as a whole. The coefficient of ferny plants (Pte-Q) is 0.95, lower than grass. Atractylodes japonica Koidz., Miscanthus sinensis Anders. var. purpurascense Rendle, and Rhododendron mucronulatum Turz. are distributed mainly on the forest bed of Pinus thunbergii. The poor distribution of Chenopodium album var. centrorubrum Makino, Setaria viridis (L.) Beauv., Amaranthus mangostanus Linne is considered attributable to the phenomenon of allellopathy caused by a chemical substance secreted from the leaves of Pinus thunbergii. The component ratio of species in this island is lower than that of other islands due probably to the small area of arable land and grassland. The poor growth of plants in the forest of Pinus thunbergii, the dominant species in this island, seems to be attributed to the low relative light intensity of the forest. Seaside plants consisting of a total of 7 species were distributed mainly in the vicinity of sandy beaches. Naturalized plants comprising a total of 11 species were relatively diversified in the number of species.

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Control Effect of Environmental-friendly Organic Materials against Major Pear Diseases (친환경 유기농자재를 이용한 배 주요병해 방제효과)

  • Yoon, Deok-Hoon;Patk, Hae-Jun;Nam, Ki-Woong
    • The Korean Journal of Pesticide Science
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    • v.14 no.4
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    • pp.401-406
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    • 2010
  • This study was conducted to investigate the control effects of the environmental-friendly materials against major diseases of pear and to elucidate factors related to spore disperse of pear scab and pear rust. Spore disperse was deeply related ambient temperature in the early stage of disease occurrence and the maximum spore disperse was affected greatly by the air humidity, Percent of infected leaves of pear scab in plot treated with Trichoderma harzianum-YC459 was 29.3% and 43.3% in plot treated with Sulfur-80%. Two formulations above-mentioned were less effective to control pear scab than the chemical pesticide, hexaconazole. Control effect in plots single- and mixed-treated with Trichoderma harzianum-YC459, Sulfur-80% and hexaconazole against pear scab were compared with that in plot treated with chemicals by conventional application schedule in pear orchard. The incidence of pear scab was lower in plot treated with the environmental-friendly materials than that of untreated plot. However there was no significant difference among the treatments at the 5% level. Application of Sulfur-80% reduced significantly the incidence of pear scab (7.8%) compared to that (10.3%) in plot treated by conventional application schedule in organically cultivated pear orchard. Alternative application of Trichoderma harzianum-YC459 and Sulfur-80% (4.3%) suppressed the incidence of pear rust than that (7.1%) in plot treated by conventional application schedule in organically cultivated pear orchard.