• Title/Summary/Keyword: Chelating

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Comparison of Antioxidant and Nitrite Scavenging Activities of Different Colored Kiwis Cultivated in Korea (국내산 키위의 색상별 항산화능 및 아질산염 소거능 비교)

  • Chung, Hai-Jung;Kim, Cheon-Jei;Choi, Yun-Sang
    • Journal of the Korean Society of Food Culture
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    • v.30 no.2
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    • pp.220-226
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    • 2015
  • This study was to conducted to investigate the antioxidative activity of 70% ethanol extracts of kiwi of three different colors (gold, green and red) by measuring DPPH, ABTS radical scavenging activity, tyrosinase inhibitory effect, metal chelating effect, reducing power, and nitrite scavenging activity. Total polyphenol contents were: gold kiwi, 3.09 mg GAE/g, green kiwi 2.71 mg GAE/g, and red kiwi 4.59 mg GAE/g, respectively. Red kiwi showed higher antioxdidant activity than gold and green kiwi. DPPH and ABTS radical scavenging activity, and nitrite scavenging activity of red kiwi exhibited 94.83, 99.57, and 97.88%, respectively, at a concentration of 20 mg/mL, which were equal to those of ascorbic acid (positive control). Metal chelating effect of red kiwi was superior to that of ascorbic acid. Therefore, the availability of red kiwi will be increased in the field of functional material for food additives and value added products.

Antioxidant Activity of Some Yogurt Starter Cultures

  • Kim, H.S.;Chae, H.S.;Jeong, S.G.;Ham, J.S.;Im, S.K.;Ahn, C.N.;Lee, J.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.2
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    • pp.255-258
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    • 2005
  • Several commercial yogurt starter cultures (L. acidophilus LA5, L. casei 01, L. acidophilus LA100, L. bulgaricus LB207 and L. rhamnosus GG744) were investigated for antioxidant activity by using in vitro assays. From the results of the present work, all strains tested showed varying degrees of antioxidant activity. L. bulgaricus LB207 showed the highest antioxidant activity, with a linoleic acid peroxidation inhibition of 81.3%. Hydroxy radical scavenging activity, ferrous iron chelating activity, reducing power and superoxide dismutase (SOD) activity were also studied. L. bulgaricus LB207 showed the highest hydroxy radical scavenging activity and L. casei 01 showed the highest chelating activity. L. bulgaricus LB207 and L. acidophilus LA100 showed good reducing power. All the strains in this study showed low SOD activity. The results of the present work suggest that antioxidant activity of L. bulgaricus LB207 due to its strong hydroxy radical scavenging activity and reducing power.

Scavenging Property of Pyungwi-san Herbal-acupuncture Solution on ROS and RNS (평위산(平胃散) 약침액(藥鍼液)의 활성산소 및 활성질소 소거능)

  • Lee, Hyo-Seung;Moon, Jin-Young
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.1
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    • pp.165-170
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    • 2007
  • Pyungwi-san(PWS) have been using as a basic prescription of digestive disorder in Korean traditional medicine. This study was performed to examine the in vitro antioxidant activity of the extract using different antioxidant tests including by 1,1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging, superoxide anion radical scavenging, metal chelating hydrogen peroxide scavenging, lipid peroxydation protective effect and scavenging effect of nitric oxide and peroxynitrite. Herbal-acupuncture solution of PWS(PWS-HS) exhibited a concentration-dependent inhibition of DPPH radical adduct formation and it showed dose-dependent free radical scavenging activity onto superoxide anions. In addition, the result of metal chelating hydrogen peroxide scavenging and ammonium thiocyanate experiments showed that PWS-HS was an active scavenger of hydroxyl radicals. Furthermore, it was also found to be effective in scavenging nitric oxide and peroxynitrite, well-known cytotoxic species that can oxidize several cellular components such as proteins, lipids and DNA.

Antioxidant Properties of Commercial Noodles Supplemented with Functional Ingredients (기능성 부재료를 첨가한 시판 국수류의 항산화 특성)

  • Son, Jong-Youn;Kang, Kun-Og
    • Journal of the East Asian Society of Dietary Life
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    • v.24 no.2
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    • pp.183-189
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    • 2014
  • We investigated noodles supplemented with functional ingredients for their antioxidant properties, including total phenol, flavonoid contents, electron donating, nitrite scavenging abilities and ferrous ion chelating effect. The total polyphenol contents of functional noodles arranged in order of decreasing concentration were kudzu (7.98%) > green tea (4.99%) > pumpkin (5.03%) > mulberry leaves (4.99%) > mugwort (4.23%) > cactus (3.57%) > kelp (3.33). The total flavonoid contents in green tea noodles were the highest as 4.35%. The electron donating ability in mugwort noodle was the highest as 12.27% at 1,000 ppm. This amount was 4.85 times than that of wheat flour noodle (2.53%). The nitrite scavenging ability of functional noodles at pH 1.2 arranged in order of decreasing concentration were green tea (66.52%) > cactus (55.12%) > kudzu (52.67%) > pumpkin (50.50%) > mulberry leaves (43.58%) > kelp (41.41%) > mugwort (37.66). The nitrite-scavenging ability of green tea noodle was lower than ascorbic acid (natural antioxidant) 77.83%, while that of green tea noodle was similar with BHT (artificial antioxidant) 69.45%. The ferrous ion chelating effect of noodles containing kelp were the highest as 27.02%. All of the experimental results showed good antioxidant property. Thus, noodles supplemented with mulberry leaves, cactus, mugwort, green tea, pumpkin, kelp or kudzu, demonstrated to have good functional effects for human health.

Comparison of Biological Activities of Synurus deltoides (Aiton) Nakai Under Different Shading Conditions

  • Jiang, Yunyao;Noh, Heesum;Wang, Myeong-Hyeon
    • Korean Journal of Plant Resources
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    • v.26 no.6
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    • pp.686-694
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    • 2013
  • Shade treatment of Synurus deltoides (Aiton) Nakai was carried out with 0, 35, and 55% shading net, and samples were marked as no shade, 35% shade, and 55% shade, respectively. We examined in vitro antioxidant and anti-inflammatory capacities using a 1,1-diphenyl-2-2-pricylhydrazyl (DPPH) radical scavenging assay, a reducing power assay, a total antioxidant assay, a metal chelating assay, a superoxide radical scavenging assay, and a nitric oxide inhibition assay. As a result, no shade and 35% shade possessed higher DPPH radical scavenging activity and reducing power ability than that of 55% shade. Notably, no shade had significantly higher total phenolic and flavonoid contents than those in the other samples. No shade exhibited significantly higher total antioxidant activity than that of 35% shade and 55% shade. However, the chelating ability of 55% shade was significantly greater than that of no shade and 35% shade; 55% shade also showed significantly higher anti-inflammatory capacity than that of no shade or 35% shade.

Antioxidant Compounds and Activities of Short-term Green Gochujang (단기속성 청고추장의 항산화 성분 및 항산화 활성)

  • Shin, Kyung-Eun;Choi, Soo-Keun;Kim, Dong-Seok;Kim, Ha-Yun
    • Journal of the East Asian Society of Dietary Life
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    • v.22 no.5
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    • pp.657-666
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    • 2012
  • The purpose of this study was to improve the quality of Korean traditional sauce products by producing short-term green gochujang with cheongyang pepper powder. To determine its antioxidant compounds and activities, we examined vitamin C, capsaicinoid, total phenolic, and total flavonoid contents, as well as electron-donating, SOD-like, ferrous ion-chelating, and nitrite-scavenging activities. Vitamin C content of short-term green gochujang was higher in CON-M than in the sample, whereas capsaicinoid content increased as the amount of cheongyang pepper powder increased. Total phenolic content, total flavonoid content, electron-donating activity, and nitrite-scavenging activity were higher in the sample than in CON-M, and the contents increased as the amount of meju powder increased. Green gochujang containing the highest amount of cheongyang pepper powder showed the highest SOD-like, and ferrous-ion chelating activities as well as superior nutrient contents, compared to red gochujang.

Elution Behavior of Copper(II) and Iron(II) Ions by Phenol Sulfonic Acid on Chelating Resin (킬레이트 수지에서 Phenol Sulfonic Acid에 의한 구리(Ⅱ)와 철(Ⅱ) 이온의 용리현상)

  • Cha, Ki Won;Hong, Jang Wook;Choi, Bae Du
    • Journal of the Korean Chemical Society
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    • v.42 no.3
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    • pp.292-296
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    • 1998
  • The elution behavior of copper(Ⅱ) and iron(Ⅱ) ions by phenol sulfonic acid(PSA) as an eluent on chelating resin, Amberlite IRC-718 have been investigated. When copper(Ⅱ) and iron(Ⅱ) solutions were adsorbed on the resin and eluted with various concentration of PSA, two peaks of each ion were appeared in the elution curve. These two peak areas were changed according to the PSA concentration. Using these phenomena, the stability constants of complex formation between the two ions and PSA were calculated. The values are 7.0 for copper(II) and $4.5{\times}10^4$ for iron(II), respectively.

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Determination of Bioactive Compounds and Antioxidant Activity of Yam (Dioscorea batatas DECNE.) on Thermal Treatment

  • Duan, Yishan;Kim, Gyeong-Hwuii;Kim, Han-Soo
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.2
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    • pp.302-311
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    • 2015
  • To better investigate the antioxidative property of thermal treatment yam (Dioscorea batatas DECNE.) in Korea, some established methods were used. 70% Methanol, 70% ethanol and chloroform-methanol (CM, 2:1, v/v) extracts were collected. 70% Methanol extract exhibited stronger antioxidative activity evaluated by ferrous ion chelating activity, NO radical scavenging activity and ${\beta}$-carotene bleaching assays. On the contrary, CM extract was the most effective in inhibiting linoleic acid peroxidation. Yam available in Korea was also analyzed for its bioactive compounds such as lycopene, chlorophyll a, b, tannin, phytic acid and total saponin contents. Total saponin was abundant in thermal treatment, which determined to be $42.52{\pm}1.88mg/g$. Based on the results obtained from this study, thermal treatment yam could be used as natural antioxidant source due to its high antioxidant activity and bioactive compound contents.

Synthesis of 2,2'-Iminodibenzoic Acid-Cellulose Adsorbent and Its Adsorptivity of Cu(II) and Pb(II) (2,2'-Iminodibenzoic acid-cellulose 흡착제의 합성과 Cu(II) 및 Pb(II)의 흡착에 관한 연구)

  • Shim, Sang-Kyun;Min, Byoung-Do
    • Analytical Science and Technology
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    • v.10 no.4
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    • pp.291-299
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    • 1997
  • 2,2'-iminodibenzoic acid-cellulose was prepared by reacting 2,2'-iminodibenzoic acid salt with cellulose-Cl obtained by chlorination of cellulose-OH which is the major component of sawdust. The adsorptivity of Pb(II) and Cu(II) was studied using the synthetic chelating adsorbent. The adsorption amounts of those ions increased with increasing pH and the optimum adsorption time of metal ion was about 1hr. The adsorptivity of Pb(II) was larger than that of Cu(II).

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