• 한국수산과학회지
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• 제44권2호
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• pp.162-166
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• 2011

The distribution and occurrence of Charybdis japonica larvae were investigated off Yeonpyong-do, Korea, in the Yellow Sea. C. japonica larvae were collected monthly at 15 stations from early June to late October in 2006 and 2007. At each station, a Bongo net with 303 and $505{\mu}m$ mesh was deployed once with a double oblique tow. No larvae were caught in June, in both years. Zoea I was predominant in late July in 2006 and early August in 2007, whereas Zoea I accounted for 84% of all larvae collected and no larval stages later than Zoea III were sampled. Megalopa were the most abundant larval stage at all stations in late August in both years. The timing of larval hatching of C. japonica may be related to that of phytoplankton blooms in the study area. The finding that Zoea I and Megalopa were predominant in the study are may indicate that C. charybdis larvae are carried by advection.

• 생명과학회지
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• 제16권4호
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• pp.672-675
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• 2006

In order to determine the morphological variations of the Japanese swimming crab, Charybdis japonica, we conducted morphological analysis of five populations that are relatively abundant in Korea and Japan. Abdominal sternite for individuals of the Tean population showed significantly short when compared to those of other populations. Principal component analysis demonstrated that morphological differentiation was due to differences (A/Q, B/M, D/P, F/O, J/K), and effectively discriminated each others. The first two components accounted for 40.9% and 60.7% of the variation, respectively. The dendrogram showed three distinct groups, Tokyo population, Taean population, and the other populations.

• 한국발생생물학회지:발생과생식
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• 제26권2호
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• pp.91-98
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• 2022

Genomic DNA (gDNA) set apart from two populations of Korean Charybdis crab (Charybdis japonica) was augmented by PCR experiments. The five oligonucleotides primers (ONT-primers) were spent to yield the number of unique loci shared to each crab population (ULSECP) and number of loci shared by the two crab populations (LSTCP). 305 fragments (FRAGs) were identified in the Charybdis crab population A (CCPA), and 344 in the Charybdis crab population B (CCPB): 44 number of ULSECP (14.43%) in the CCPA and 110 (31.98%) in the CCPB. 44 number of LSTCP, with an average of 8.8 per primer, were detected in the two crab populations. The bandsharing (BS) value between entity's no. 01 and no. 10 was the lowest (0.371) between the two CCPs. The average bandsharing (ABS) values of individuals in the CCPA (0.575±0.014) were lesser than in those originated from the CCPB (0.705±0.011) (p < 0.05). The polar hierarchical dendrogram (PHD) achieved by the five ONT-primers denotes three genetic clusters (GCs): cluster I (CHARYBCRAB 01, 04, 05, 06, and 08), cluster II (CHARYBCRAB 02, 03, 07, 09, 10, and 11) and cluster III (CHARYBCRAB 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, and 22). The shortest genetic distance (GD) displaying significant molecular difference (MD) was between individuals CHARYBCRAB no. 18 and CHARYBCRAB no. 17 (0.055).

• 환경생물
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• 제27권3호
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• pp.297-305
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• 2009

본 연구는 갯벌을 이용하는 민꽃게(Charybdis japonica) 개체군의 섭식생태를 파악하기 위하여 수행되었다. 표본 채집을 위한 현장조사는 1999년 11월부터 2001년 1월까지 매월별로 변형된 오타트롤(modified otter trawl)을 이용하여 실시하였다. 섭식특성 파악을 위한 위내용물 분석은 총 273개체를 대상으로 하였다. 위내용물 분석 결과, 본 종의 주된 먹이생물은 절지동물문 갑각류의 게류 (Brachyura), 만각류 (Cirripedia), 저서성 요각류(Harpacticoid copepoda), 극피동물문의 거미불가사리류(Ophiuroidea) 및 연체동물문의 이매패류(Bivalvia)와 두족류 (Cephalopoda) 등이었다. 민꽃게의 생물학적 특성(성 성숙에 이르는 정도)을 기초로 성장에 따른 먹이생물의 변화를 파악하기 위하여 2개의 크기군(${\leq}60mm$와 ${\geq}61mm$ 갑폭)으로 구분하여 분석한 결과, 소형 크기 개체는 중형저서동물인 저서성 요각류를, 중 대형 크기 개체는 이매패류와 게류를 주된 먹이원으로 하였다. 이상에서와 같이 생물학적 특성으로 구분된 2개의 크기군에 따라 먹이생물의 조성에는 차이가 있었고, 이것은 성장에 따라 점차적으로 먹이습성이 변화함을 시사하는 것으로 판단할 수 있었다.

• Environmental Analysis Health and Toxicology
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• 제29권
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• pp.2.1-2.7
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• 2014

Objectives Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of protein. In this study, an HSP90 gene was isolated from Asian paddle crab, Charybdis japonica, as a bio-indicator to monitor the marine ecosystem. Methods This work reports the responses of C. japonica HSP90 mRNA expression to cellular stress by endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA) and 4-nonylphenol (NP) using real-time. reverse transcription polymerase chain reaction. Results The deduced amino acid sequence of HSP90 from C. japonica shared a high degree of homology with their homologues in other species. In a phylogenetic analysis, C. japonica HSP90 is evolutionally related with an ortholog of the other crustacean species. The expression of HSP90 gene was almost distributed in all the examined tissues of the C. japonica crab but expression levels varied among the different body parts of the crabs. We examined HSP90 mRNA expression pattern in C. japonica crabs exposed to EDCs for various exposure times. The expression of HSP90 transcripts was significantly increased in C. japonica crabs exposed to BPA and NP at different concentrations for 12, 24, 48 and 96 hours. The mRNA expression of HSP90 gene was significantly induced in a concentration- and time-dependent manner after BPA or NP exposures for 96 hours. Conclusions Taken together, expression analysis of Asian paddle crab HSP90 gene provided useful molecular information about crab responses in stress conditions and potential ways to monitor the EDCs stressors in marine environments.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2005년도 춘계학술발표대회 논문요약집
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• pp.124-125
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• 2005

• 수산해양교육연구
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• 제20권1호
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• pp.95-106
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• 2008

민꽃게(Charybdis japonica)를 이용하여 만든 게장은 우리의 식생활에 밀접하게 접근되어 있으나 이에 관련된 연구는 미비한 실정이며 지역마다 게장을 만드는데 필요한 장에 여러 가지 양념류가 첨가되어 있어 게장 본래의 화학적 성분 조성을 밝히기 쉽지 않을 것으로 사료된다. 이에 본 연구에서는 민꽃게의 화학적 성분 조성과 염지한 민꽃게장을 온도별($5{^{\circ}C}$, $15{^{\circ}C}$, 상온)로 저장하여 저장일수(10, 15, 20, 25, 30일)에 따른 화학성분 변화량을 측정하였으며 실험한 결과를 요약하면 다음과 같다. 1. 민꽃게의 일반성분 함량을 보면 수분(77.5％), 회분(2.3％), 조단백(19.7％), 조지방(0.5％)이었으며 저장기간 중 수분과 조단백질 및 조지방 함량은 감소하는 경향을 보인 반면 회분 함량은 수분 함량의 감소에 따라 상대적으로 그 함량이 증가하는 경향을 나타내었다. 2. 민꽃게와 민꽃게장의 휘발성 염기질소량은 민꽃게는 2.3mg/100g로 나타났으며 $5{^{\circ}C}$에서 저장한 민꽃게장에서는 30일 경과 후에도신선도(20.8mg/100g)를 유지함을 알 수 있었으나 $15{^{\circ}C}$에서 저장한 민꽃게에서는 25일 이후에는 초기부패의 상태(31.2mg/100g)를 나타내었다. 상온에서는 민꽃게장 저장 10일만에 초기부패의 상태(34.7mg/100g)를 나타내었으며 30일 경과 시 부패취(51.4mg/100g)를 내었다. 이로서 민꽃게장의 저장은 상온보다는 $5{^{\circ}C}$, $15{^{\circ}C}$등 비교적 낮은 온도에서 일정하게 온도를 유지할 수 있는 장소에서 보관 하는 것이 효과적임을 알 수 있었다. 3. 민꽃게와 민꽃게장의 콜레스테롤 함량을 보면 생시료의 콜레스테롤 함량은 100mg/100g로 나타났으며 민꽃게장의 저장 중 콜레스테롤 변화를 살펴보면 $5{^{\circ}C}$에서는 저장기간 중 변화량이 적었는데 건물량으로 환산하여 보면 민꽃게에서 콜레스테롤 함량이 444.4mg/100g였으나 저장일수 10일에 306.3mg/100g, 저장일수 30일에 395.3mg/100g로 약 11-31％ 감소하였으며 $15{^{\circ}C}$에서 저장한 민꽃게장에서는 저장기간 중 콜레스테롤 함량이 다소 증가하였으나 건물량으로 환산하면 저장일수 10일에 272.0mg/100g, 저장일수 30일에 440.6mg/100g로 약 0.8-38.76％로 감소하였다. 상온에서 저장한 민꽃게장에서도 $5{^{\circ}C}$와 $15{^{\circ}C}$와 유사한 경향을 보였는데 건물량으로 환산해보면 저장일수 10일에 332.2mg/100g, 저장일수 30일에 408.9mg/100g로 약 8-25％ 감소함을 알 수 있었다. 4. 민꽃게의 유리아미노산 함량을 보면 hydroxyproline(1015.8mg/100g), urea(731.2mg/100g), arginine(256.6mg /100g), taurine(215.9mg/100g), glutamic acid(109.7mg/100g), alanine (106.3mg/100g)의 순서로 정량되었으며 glutamic acid(109.7mg/100g), alanine (106.3mg/100g)의 순서로 정량되었으며, 민꽃게장에서도 이들 아미노산의 함량은 각 온도대별($5{^{\circ}C}$, $15{^{\circ}C}$, 상온), 저장기간(10, 15, 20, 25, 30일)에 따라 양적변화는 약간 있지만 대체로 그 함량이 많았다. 정미성분인 glutamic acid, glycine, alanine 및 lysine의 함량을 보면 $5{^{\circ}C}$에서 저장한 민꽃게장에는 저장일수 25일 경과 시, $15{^{\circ}C}$에서 저장한 민꽃게장에는 저장일수 15일 경과 시, 상온에서 저장한 민꽃게장에는 저장일수 20일 경과 시 다른 저장일수에 비해 이들 아미노산의 함량이 많은 것으로 나타났다. 5. 민꽃게에서 타우린 함량은 215.9mg/100g로 나타났으며 $5{^{\circ}C}$에서 저장한 민꽃게장에서는 저장기간 동안 그 함량이 감소하는 경향을 나타냈다. $15{^{\circ}C}$와 상온에서도 유사한 경향을 나타 내었으 며, $15{^{\circ}C}$에서 저장한 민꽃게장의 타우린 함량의 변화폭이 가장 적은 것으로 나타났다.

• 해양환경안전학회지
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• 제20권3호
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• pp.259-269
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• 2014

The objectives of this study to determine seasonal changes in species composition and abundance of fish and decapod assemblage, and the relationships between environmental factors and their abundance in an eelgrass bed of Jindong Bay. A total of 26 fish species and 29 decapod species were collected by a small beam trawl from an eelgrass bed in Jindong Bay in 2002. The dominant fish species were Hexagrammos otakii, Pholis neulosa and P. fangi and these accounted for 48.4 % in the total number of individuals. Dominant decapod taxa were Palaemon macrodactylus, Charybdis japonica, Pagurus minutus and C. bimaculata. These were primarily small species or early juveniles of larger species. Species composition and abundance varied greatly showing a peak in the number of individuals in April and May, and peak biomass in fish in July and decapods in August. Catch rate was low in winter months both in fish and in decapod. Seasonal changes in the abundance of fishes and decapods corresponded with eelgrass biomass and abundance of food organisms indirectly.

• 대한본초학회지
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• 제35권4호
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• pp.17-23
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• 2020

Objectives : In this study, we investigated the protective effects of Charybdis japonica (C. japonica) water extract on the acute reflux esophagitis in rat models. Methods : Twenty rats were divided into four groups for examination: normal control group (n=6), the reflux esophagitis group (n=6), reflux esophagitis treated with positive control group (ranitidine 40 mg/kg, n=6), reflux esophagitis treated with C. japonica group (100 mg/kg, n=6). All rats fasted for 18 hr and then were induced with reflux esophagitis by a pylorus and forestomach ligation operation. After 4 hr, the rats were sacrificed. The proinflammatory cytokine and proteins expression measured by western bolt assay, and the histopathological analysis of the esophageal mucosa measured by hematoxylin and eosin staining. Results : C. japonica administration significantly was protecting esophageal mucosal damage upon histological analysis of reflux esophagitis in rats. The C. japonica treatment confirmed the protection of the reduction of claudin-5, an evaluation index of the damage of tight junctions in the reflux esophagitis. C. japonica was also found to inhibit the expression of proteins such as COX-2 and TNF-α in the rat esophagus. C. japonica markedly attenuated the activation of NF-κB and phosphorylation of IκBα at the same time. Conclusion : These results indicated that C. japonica suppressed the development of esophagitis through the modulation of inflammation by regulating NF-κB activation. Based on these findings, we concluded that C. japonica can prevent reflux esophagitis.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2002년도 춘계 수산관련학회 공둥학술발표회
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• pp.504-505
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• 2002