• Title/Summary/Keyword: Channel Composition

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Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes

  • Cuong, Dang Van;Kim, Na-Ri;Kim, Eui-Yong;Lee, Young-Suk;Kim, Hyun-Ju;Kang, Sung-Hyun;Hur, Dae-Young;Joo, Hyun;Park, Young-Shik;Hong, Yong-Geun;Lee, Sang-Kyung;Chung, Joon-Yong;Seog, Dae-Hyun;Han, Jin
    • The Korean Journal of Physiology and Pharmacology
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    • v.8 no.4
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    • pp.201-206
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    • 2004
  • Mitochondrial ATP-sensitive potassium $(mitoK_{ATP})$ channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of $mitoK_{ATP}$ channels remains unclear. In this study, we investigated the subunit composition of $mitoK_{ATP}$ channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while $mitoK_{ATP}$ channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of $mitoK_{ATP}$ channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands $({\sim}44,\;{\sim}46,\;and\;{\sim}30\;kDa)$ were detected, and a specific ${\sim}46-kDa$ immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of $mitoK_{ATP}$ channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.

Influence of Co-sputtered HfO2-Si Gate Dielectric in IZO-based thin Film Transistors (HfO2-Si의 조성비에 따른 HfSiOx의 IZO 기반 산화물 반도체에 대한 연구)

  • Cho, Dong Kyu;Yi, Moonsuk
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.2
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    • pp.98-103
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    • 2013
  • In this work, we investigated the enhanced performance of IZO-based TFTs with $HfSiO_x$ gate insulators. Four types of $HfSiO_x$ gate insulators using different diposition powers were deposited by co-sputtering $HfO_2$ and Si target. To simplify the processing sequences, all of the layers composing of TFTs were deposited by rf-magnetron sputtering method using patterned shadow-masks without any intentional heating of substrate and subsequent thermal annealing. The four different $HfSiO_x$ structural properties were investigated x-ray diffraction(XRD), atomic force microscopy(AFM) and also analyzed the electrical characteristics. There were some noticeable differences depending on the composition of the $HfO_2$ and Si combination. The TFT based on $HfSiO_x$ gate insulator with $HfO_2$(100W)-Si(100W) showed the best results with a field effect mobility of 2.0[$cm^2/V{\cdot}s$], a threshold voltage of -0.5[V], an on/off ratio of 5.89E+05 and RMS of 0.26[nm]. This show that the composition of the $HfO_2$ and Si is an important factor in an $HfSiO_x$ insulator. In addition, the effective bonding of $HfO_2$ and Si reduced the defects in the insulator bulk and also improved the interface quality between the channel and the gate insulator.

Distribution Patterns of the Benthic Macrofaunal Community in the Coastal Area of Inchon, Korea (인천연안역 저서동물군집의 시.공간적 분포 양상)

  • Koh, Byoung-Seol;Lee, Jae-Hac;Hong, Jae-Sang
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.2 no.1
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    • pp.31-41
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    • 1997
  • This study was carried out to investigate the composition and the distribution of the benthic community in the coastal area of Inchon seasonally from May 1994 to February 1995, and to estimate the environmental factors that effect the temporal and the spatial changes of benthic species composition. In the present study, 231 species were collected with the average density of 455 ind./$m^2$. The dominant species were Heteromastus sp., Sternaspis scutata, Chaetozone setosa, Mediomastus sp., Glycinde sp., Glycera sp. and Nephtys oligobranchia, which are in the polychaete group; abundance of these species showed seasonal and spatial variations. The study area was divided into 4 regions by cluster analysis such as outside area of Palmido (I), main channel region and adjacent are of Shihwa sea wall (II), north-eastern area of Youngjongdo (III), and the Inchon harbor and the mouth area of Sorae harbor (IV). The particle size of sediment which generally influences the distribution of the benthic community, the differences of the concentration of heavy metals, and of the dissolved oxygen, the increase of the proportion of polychaetes and molluscs, the absence of the crustacean phoxocephalids, and the increase of the abundance of pollution indicator species, indicated that the environment of the areas have been degrading gradually from Group I to Group IV.

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Changes of Epilithic Diatom Communities according to Urbanization Influence in the Pocheon and Youngpyeong Streams (도시화 정도에 따른 포천천과 영평천의 돌 부착규조 군집 변화)

  • Kim, Yong-Jae
    • Korean Journal of Ecology and Environment
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    • v.40 no.3
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    • pp.468-480
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    • 2007
  • The urbanization is altering the hydrology, water quality, channel form of waterway and changing the composition of biological communities in the aquatic ecosystem. Recently, towns grew bigger by the drift of large numbers of people and the medium and small leather and dyeing industries around the Pocheon and Youngpyeong streams. The discharges of sewage were increased by them. The UII (urban intensity index) was 85 (st. P-3) and 91 (st. P-6) in the Pocheon stream and about 20 in the Youngpyeong stream. A total 141 taxa of epilithic diatoms which were composed of 2 order, 8 family, 30 genera. Dominant species were Navicula saprophila, N. subminuscula, Nitzschia palea, Gomphonema pseudoaugur in the Pocheon stream and Achnanthes alteragracillima, A. convergens, A. minutissima, N. minima, N. fonticola, N. frustulum and Cymbella minuta var. silesiaca in the Youngpyeong stream. It Showed the different composition of dominant species by the urbanization near two streams. In the relationships between UII and environmental factors such as EC, BOD, COD, TN, TP and DAIpo, UII showed the high relations $(r^2>0.8)$. It was the difference of organic pollution according to urbanization. It therefore, was higher the relative abundance and more the numbers of saprophilous taxa in the Pocheon stream than the Youngpyeong stream. The water quality of two streams by biological indicators(DAIpo) was polysaprobic state(st. P-3, P-4, P-5) in the Pocheon stream and was oligosaprobic (Y-1), mesosaprobic (Y-2, 3) and polysaprobic state (Y-4) in the Youngpyeong stream during the investigation periods.

A Study on the Cultural Function of Space and Place: Its Focus on Travel Program (공간과 장소의 문화적 기능: 여행 프로그램 <어서와 한국은 처음이지?>를 중심으로)

  • Hahm, Hyun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.3
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    • pp.168-175
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    • 2019
  • The purpose of this paper is to examine the characteristics of organic relations according to the characteristics of space and place in which human activities are spreading. The diffusion of various human experiences and the interaction of space and place that form a mutual construction network create momentum for expanding individual or collective experiences. The space and place that lead to the interactions of human activities are expressed in various ways. Through the domestic travel program especially, the ways of expressing space and place are spreading as various "fields of experience." The composition and the expression system of a travel program is transformed into a subject of thought, not a mere experience of space and place through the formation of a "field of experience" that can be thought out and thought of as an existing travel style. Therefore, in this study, we examine the characteristics of the MBC Plus channel from a socio-cultural perspective, expressed through the experience of space and place characteristics expressed through a travel program. The result of an analysis of story development composition for this study shows that the experience of space and place minimizes the boundaries of various cultural discounts according to their relationships to food and communication. In addition, the analysis results of a three-way communication structure show that the emotional code of the audience is maximized through the process of maximizing the effect of reality, and minimizing the gap between the cultural differences and the boundaries through the empathic situation and the empathic subject.

Characteristics of the Species Composition by Plant Community in the Shincheon Wetland of Mangyeong River, Jeonbuk (만경강 신천습지의 식물군락별 종조성적 특성)

  • Kwang-Jin, Cho;Jung-A, Lee;Jeoncheol, Lim;Yeounsu, Chu
    • Journal of Environmental Impact Assessment
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    • v.31 no.6
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    • pp.409-422
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    • 2022
  • Riverine wetlands are an important element of the river ecosystem and account for approximately 38% of the inland wetlands surveyed so far. The Shincheon Wetland located in Mangyeong River is also a channel wetland as the flow rate is slowed by the constructed weirs, leading to sediment accumulation. To identify the conservation value and ecological characteristics of Shincheon Wetland, its vegetation and plant diversity were identified using a phytosociological method, and a total of 45 vegetation-related datasets were collected. Overall, 24 plant communities, comprising a total of 153 taxa (49 families, 117 genera, 146 species, 2 subspecies, 5 varieties) were identified. The plant with the highest appearance rate in the communities was Humulus japonicus Siebold & Zucc. In addition, annual herb species, including Rumex crispus L., Bromusjaponicus Thunb., Erigeron annuus (L.) Pers., and Artemisia indica Willd. were frequently observed to be growing in the secondary grassland. Naturalized plants were surveyed in the 38 taxa; the urbanization index was 10.3% and the naturalized index was 24.8%. Plant communities were largely classified into submerged vegetation, floating and floating-leaved vegetation, annual and biennial vegetation, perennial herb vegetation, and woody vegetation. The distribution of plant communities reflecting various habitats, including the lentic and lotic zone maintaining a constant water depth, littoral zone experiencing intermittent water level fluctuations, and dry floodplain environment was also confirmed. Overall, plant community development plays an important role in the habitat for wild animals; therefore, it is expected to positively impact biodiversity enhancement.

Comparison of chemical compositions and source apportionmentof PM1.0 and PM2.5 in Seoul and Gwangju in 2021 (2021년 서울과 광주 지역 PM1.0과 PM2.5의 화학적 특성 비교 분석 연구)

  • Ju Young Kim;Seung Mee Oh;Hye Jung Shin;Yu Woon Chang;Yong Hwan Lee;Su Jin Kwon;Sung Deuk Choi;Sang Jin Lee;Ji Yi Lee
    • Particle and aerosol research
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    • v.19 no.4
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    • pp.129-144
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    • 2023
  • The PM1.0 and PM2.5 samples were collected synchronously using a single channel particulate sampler equipped with PM1.0 and PM2.5 cyclones, respectively, and seasonal mass concentration and chemical composition of PM1.0 and PM2.5 were quantified in Seoul and Gwangju in 2021-2022. The mass concentrations of PM1.0 and PM2.5 were 17±11 and 22±14 ㎍/m3 in Seoul, and 16±9 and 19±12 ㎍/m3 in Gwangju, respectively. The average ratios of PM1.0/PM2.5 were 83±16% in Seoul and 83±7% in Gwangju. The chemical compositions of PM1.0 and PM2.5 were similar at both sites with OC component being the most dominant, and NO3- increasing from summer to winter, while, the difference of chemical distribution at the two sites was most distinct in the autumn. Gwangju showed a higher proportion of OC and a lower proportion of NO3- compared to Seoul during the autumn. Both sites appear to reflect their urban characteristics, with Gwangju also reflecting the impact of biomass combustion as a part of rural activities.

Correlation of the Rates of Solvolyses of Cinnamyl Bromide

  • Koo, In-Sun;Cho, Jun-Mi;An, Sun-Kyoung;Yang, Ki-Yull;Lee, Jong-Pal;Lee, I.
    • Bulletin of the Korean Chemical Society
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    • v.24 no.4
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    • pp.431-436
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    • 2003
  • Solvolytic rate constants at 25℃ are reported for solvolyses of cinnamyl bromide (1) in binary mixtures of water with acetone, ethanol, methanol, methanol-d, and 2,2,2-trifluoroethanol. Product selectivities are reported for solvolyses of 1 in aqueous ethanol and methanol. Rate ratios in solvents of the same $Y_{Br}$ value and different nucleophilicity provide measures of the minimum extent of nucleophilic solvent assistance (e.g. $[k_{40EW}/k_{97TFE}]$Y = 2.88, EW = ethanol-water). With use of the extended Grunwald-Winstein equation, the l and m values are similar to the values of 0.43 and 0.88 obtained for the solvolyses of 1 using the equation (see below) which includes a parameter (I) for solvation of aromatic rings. The magnitude of l and m values associated with a change of solvent composition predicts the $S_{N1}$ reaction mechanism rather than an $S_{N2}$ channel. Product selectivities (S), defined by S = [ether product]/[alcohol product]×[water]/[alcohol solvent] are related to four rate constants for reactions involving one molecule of solvent as nucleophile and another molecule of solvent as general base catalyst. A linear relationship between 1/S and molar ratio of solvent is derived theoretically and validated experimentally for solvolyses of the above substrates from water up 75% 1/S = $(k_{wa}/k_{aw})$([alcohol solvent]/[water]) + $k_{ww}/k_{aw}$ alcohol-water. The results are best explained by product formation from a “free” carbocation intermediate rather than from a solvent-separated ion pair.

Characteristics of HfO2-Al2O3 Gate insulator films for thin Film Transistors by Pulsed Laser Deposition

  • Hwang, Jae Won;Song, Sang Woo;Jo, Mansik;Han, Kwang-hee;Kim, Dong woo;Moon, Byung Moo
    • Proceedings of the Korean Vacuum Society Conference
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    • 2016.02a
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    • pp.304.2-304.2
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    • 2016
  • Hafnium oxide-aluminum oxide (HfO2-Al2O3) dielectric films have been fabricated by Pulsed Laser Deposition (PLD), and their properties are studied in comparison with HfO2 films. As a gate dielectric of the TFT, in spite of its high dielectric constant, HfO2 has a small energy band gap and microcrystalline structure with rough surface characteristics. When fabricated by the device, it has the drawback of generating a high leakage current. In this study, the HfAlO films was obtained by Pulsed Laser Deposition with HfO2-Al2O3 target(chemical composition of (HfO2)86wt%(Al2O3)14wt%). The characteristics of the thin Film have been investigated by x-ray diffraction (XRD), atomic force microscopy (AFM) and spectroscopic ellipsometer (SE) analyses. The X-ray diffraction studies confirmed that the HfAlO has amorphous structure. The RMS value can be compared to the surface roughness via AFM analysis, it showed HfAlO thin Film has more lower properties than HfO2. The energy band gap (Eg) deduced by spectroscopic ellipsometer was increased. HfAlO films was expected to improved the interface quality between channel and gate insulator. Apply to an oxide thin Film Transistors, HfAlO may help improve the properties of device.

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A Case Study on the Nonverbal Immediacy of the Robot (로봇의 비언어적 즉시성에 대한 사례연구)

  • Jeong, Seongmi;Shin, Dong-Hee;Gu, Jihyang
    • The Journal of the Korea Contents Association
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    • v.15 no.7
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    • pp.181-192
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    • 2015
  • Nonverbal immediacy plays a key role in interpersonal communication, inducing closeness and another interaction. This case study investigates the nonverbal behaviors in Human-Robot Interaction(HRI) focusing on immediacy, and how they affect perception of a robot. The results show that nonverbal immediacy, such as nodding and leaning forward, affect perceived interactivity. Nonverbal immediacy can be interpreted their meaning clearly when verbal feedback or other communication channel reinforcing them. Also, touch is found to affect come up with similar ones to the robot, because it is associated with concrete and discrete context. People tend to apply social rules to the robot, but they are more open to the unfamiliar robot compared to strangers. The findings in this study provide future HRI studies with heuristic implications by showing a direction and clarifying principles for composition of verbal and nonverbal expression.