• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.3843-3847
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• 2015

Background: Cervical cancer is the second most common cause of cancer related death of women. Persistent HPV infection, especially with high-risk types such as HPV16 and HPV18, has been identified to be the primary cause of cervical cancer. E6 and E7 are the major oncoproteins of high-risk HPVs, which are expressed exclusively in HPV infected tissues, and thereby represent ideal therapeutic targets for immunotherapy of cervical cancer. Materials and Methods: In this work, we used recombinant adenovirus expressing coden-optimized HPV16 E6 and E7 fusion protein (Ad-ofE6E7) to prime dendritic cells (DC-ofE6E7), to investigate the ability of primed DC vaccine in eliciting antitumor immunity in vitro and vivo. Results: Our results indicated that DC-ofE6E7 vaccine co-culturing with splenocytes could strongly induce a tumor-specific cytotoxic T lymphocyte (CTL) response and kill the TC-1 cells effectively in vitro. Moreover, DC-ofE6E7 vaccine induced protective immunity against the challenge of TC-1 cancer cells in vivo. Conclusions: The results suggested that the HPV16 ofE6E7 primed DC vaccine has potential application for cervical cancer immunotherapy.

• Journal of Veterinary Clinics
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• v.36 no.1
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• pp.23-29
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• 2019

While research findings suggest that the highly pathogenic avian influenza (HPAI) is the leading cause of economic loss in Korean poultry industry with an estimated cumulative impact of $909 million since 2003, identifying the environmental and anthropogenic risk factors involved remains a challenge. The objective of this study was to identify areas at high risk for potential HPAI outbreaks according to the likelihood of HPAI virus detection in wild birds. This study integrates spatial information regarding HPAI surveillance with relevant demographic and environmental factors collected between 2003 and 2018. The Maximum Entropy (Maxent) species distribution modeling with presence-only data was used to model the spatial risk of HPAI virus. We used historical data on HPAI occurrence in wild birds during the period 2003-2018, collected by the National Quarantine Inspection Agency of Korea. The database contains a total of 1,065 HPAI cases (farms) tied to 168 unique locations for wild birds. Among the environmental variables, the most effective predictors of the potential distribution of HPAI in wild birds were (in order of importance) altitude, number of HPAI outbreaks at farm-level, daily amount of manure processed and number of wild birds migrated into Korea. The area under the receiver operating characteristic curve for the 10 Maxent replicate runs of the model with twelve variables was 0.855 with a standard deviation of 0.012 which indicates that the model performance was excellent. Results revealed that geographic area at risk of HPAI is heterogeneously distributed throughout the country with higher likelihood in the west and coastal areas. The results may help biosecurity authority to design risk-based surveillance and implementation of control interventions optimized for the areas at highest risk of HPAI outbreak potentials.

• Journal of fish pathology
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• v.22 no.1
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• pp.45-52
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• 2009

Zebrafish was firstly applied to an experimental model for scuticociliatosis caused by Philasterides dicentrarchi, a facultative parasitic ciliate in cultured marine fish. The susceptibility of zebrafish to infection of P. dicentrarchi was assessed by intraperitoneal injection of the ciliates, which produced typical symptoms of scuticociliatosis and significant mortality. The potential use of zebrafish as a model to evaluate the vaccine efficacy against scuticociliatosis was analyzed by immunization of zebrafish with the ciliates lysate. Furthermore, the effect of different adjuvants, such as Quillaja saponin (QS), Montanide, and Freund’s incomplete adjuvant (FIA) on the protective efficacy of the vaccine was investigated. Groups of zebrafish injected with QS or Montanide alone showed higher survival of fish against challenge test compared to control fish. The results suggest that adjuvant-mediated enhancement of innate immune responses play important roles in protection of fish against scuticociliatosis. The considerably high survival in the fish immunized with the antigen alone indicates that the ciliate lysate itself is highly immunogenic to zebrafish, which can elicit protective immune responses. The protective potential of the antigen, ciliate lysate, was enforced through combined administration with adjuvants including QS, Montinide and FIA. No or low mortalities in the groups of fish immunized with the antigen plus adjuvants suggests that the adaptive immune responses of zebrafish might be accelerated by the adjuvants or the protective potential of the antigen and adjuvants might synergistically interact. In spite of several shortcomings such as difficulties in sampling of serum and leucocytes enough to routine immunological analyses, zebrafsih might be the most convenient experimental animal for scuticociliatosis.

• Journal of Information Processing Systems
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• v.18 no.3
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• pp.359-373
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• 2022

The new crown pneumonia (COVID-19) has become a global epidemic. The disease has spread to most countries and poses a challenge to the healthcare system. Contact tracing technology is an effective way for public health to deal with diseases. Many experts have studied traditional contact tracing and developed digital contact tracking. In order to better understand the field of contact tracking, it is necessary to analyze the development of contact tracking in the field of computer science by bibliometrics. The purpose of this research is to use literature statistics and topic analysis to characterize the research literature of contact tracking in the field of computer science, to gain an in-depth understanding of the literature development status of contact tracking and the trend of hot topics over the past decade. In order to achieve the aforementioned goals, we conducted a bibliometric study in this paper. The study uses data collected from the Scopus database. Which contains more than 10,000 articles, including more than 2,000 in the field of computer science. For popular trends, we use VOSviewer for visual analysis. The number of contact tracking documents published annually in the computer field is increasing. At present, there are 200 to 300 papers published in the field of computer science each year, and the number of uncited papers is relatively small. Through the visual analysis of the paper, we found that the hot topic of contact tracking has changed from the past "mathematical model," "biological model," and "algorithm" to the current "digital contact tracking," "privacy," and "mobile application" and other topics. Contact tracking is currently a hot research topic. By selecting the most cited papers, we can display high-quality literature in contact tracking and characterize the development trend of the entire field through topic analysis. This is useful for students and researchers new to field of contact tracking ai well as for presenting our results to other subjects. Especially when comprehensive research cannot be conducted due to time constraints or lack of precise research questions, our research analysis can provide value for it.

• Journal of Periodontal and Implant Science
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• v.34 no.2
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• pp.425-448
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• 2004

A bioactive and degradable poly(epsilon -caprolactone)/silica nanohybrid(PSH) was synthesized for the application as a bone substitute. PSH was manufactured by using silica and polycaprolacton. PSH was manufactured in some composition after low crystaline apatite had been formed in simulated body fluid and, was used this study. The safety of the PSH was established by test of acute, and subacute toxicity, sensitization cytotoxicity and sterility. In order to assess activity of osteoblast, the test for attaching osteoblast, proliferation test for osteoblast, differentiating gene expression test are performed in vitro. And bone substitutes were grafted in rabbit's calvarium, during 8 weeks for testing efficacy of bone substitutes. Degree of osteogenesis and absorption of substitutes were evaluated in microscopic level. In result, it was not appeared that acute and subacute toxicity, sensitization in intradermal induction phase, topical induction phase and challenge phase. It was shown that the test can not inhibit cell proliferation. adversely, it had some ability to accelerate cell proliferation. The result of sterility test described bacterial growth was not detected in most test tube. The attaching and proliferation test of osteoblast had good results. In the result of differentiating gene expression test for osteoblast, cbfa1 and, alkaline phosphatase, osteocalcin and GAPDH were detected with mRNA analysis. In the PSH bone formation test, ostgeoblastic activity would be different as material constitution but it had good new bone formation ability except group #218. futhermore, some material had been absorbed within 8 weeks. Above studies, PSH had bio-compatibility with human body, new bone formation ability and accelerate osteoblastic activity. So it would be the efficient bone substitute material with bio-active and biodegradable.

• Journal of Environmental Impact Assessment
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• v.4 no.3
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• pp.27-29
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• 1995

Within regional and municipal planning we are using several levels or types of EIA in the city of Dresden. Some of these types, practical aspects and some experiences of our work will be presented in this contribution. Firstly I may introduce you to some general conditions for your better understanding of our principles of work. Surely you know about. the destruction of the political and economical structures in Eastern Germany since 1989. Until today our not quite simple task is to build up new ones. At the same time people were in great expectation of freedom and high standard of living as soon as possible. Economical difficulties increased in association with the breakdown of the market in Eastern Europe. How to rebuild industrial estatements and how to renew the traffic systems? We had to find answers to all these complex question. Should we only repair the former damages or could we reach a really environmental sound production for the future? The demand for a rapid economic growth is an incredible challenge for the application of new environmental ideas. I am truly not sure whether you know the city of Dresden or not. So I would like to give you a short introduction. Dresden is situated in a valley shaped by the river Elbe. There live about 500,000 people. Dresden has a great reputation for arts and sciences. Its also well know as a town of high technology industries such as electronics and optics. We restored the power plant and therefore we don't need any atomic power plants actually we haven't got one. Since 1990 there were founded many official agencies in Dresden because it is the capatal of Saxony. Considering nature and environment we there is a large forest area called "Dresdner Heide". The river Elbe and the meadows are situated on both sides of the river. There are a lot of green and free places in the city area too. Furtheron there is something unusual for a large city: about 50% of the drinking water resources mostly take place within the city itself. The origin is the ground water as well as water from the river Elbe after filtration of course.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.8
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• pp.395-401
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• 2015

Recently, many research studies have been carried out on the efficiency of light-shelf daylighting systems, especially comparing performance improvements and the limitations of reflective surfaces and their lighting performance. In this study, a crystal face reflective surface is proposed. The objective of the study is to evaluate the lighting performance of a crystal face light-shelf through a performance study. The performance study was carried out in a full scale test-bed in order to calculate the light distribution and energy consumption utilizing the standard indoor illumination as an index. The conclusions of the performance study are as follows. 1) The optimal angle of incidence for daylighting for both the operable flat type light-shelf and the crystal face light-shelf are taken in the natural environment on the dates of the winter and summer solstices, as well as the autumn and spring equinoxes. 2) The application and installation of the crystal face light-shelf can produce a 29.9%~34.3% increase of light distribution within the indoor space. However, the increase of light distribution can also lead to a decrease in the uniformity ratio, a design challenge that should be considered when applying a crystal face light-shelf. 3) It is possible to achieve a 7.98%~13.3% greater reduction in energy consumption when applying a crystal face light-shelf than when applying a flat type light-shelf. The increase in the number of crystal faces should concur with the analysis of the energy reduction. A limitation of the study is that only one predetermined pattern was performance tested for a crystal face light-shelf. In order to carry out further research on crystal face light-shelves, additional performance studies are needed based on alternative patterns and designs.

• Advances in aircraft and spacecraft science
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• v.10 no.3
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• pp.223-243
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• 2023

There is a burgeoning demand for minimizing the mass of satellites because of its direct impact on reducing launch-to-orbit cost. This must be done without compromising the structure's efficiency. The present paper introduces a relatively low-cost and easily implementable approach for optimizing structural mass to a maximum natural frequency. The natural frequencies of the satellite are of utmost pertinence to the application requirements, as the sensitive electronic instrumentation and onboard computers should not be affected by the vibrations of the satellite structure. This methodology is applied to a realistic model of Al-Azhar University micro-satellite in partnership with the Egyptian Space Agency. The procedure used in structural design can be summarized in two steps. The first step is to select the most favorable primary structural configuration among several different candidate variants. The nominated variant is selected as the one scoring maximum relative dynamic stiffness. The second step is to use perforation patterns reduce the overall mass of structural elements in the selected variant without changing the weight. The results of the presented procedure demonstrate that the mass reduction percentage was found to be 39% when compared to the unperforated configuration that had the same plate thickness. The findings of this study challenge the commonly accepted notion that isogrid perforations are the most effective means of achieving the goal of reducing mass while maintaining stiffness. Rather, the study highlights the potential benefits of exploring a wider range of perforation unit cells during the design process. The study revealed that rectangular perforation patterns had the lowest efficiency in terms of modal stiffness, while triangular patterns resulted in the highest efficiency. These results suggest that there may be significant gains to be made by considering a broader range of perforation shapes and configurations in the design of lightweight structures.

• Journal of Korean Institute of Industrial Engineers
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• v.41 no.1
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• pp.34-42
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• 2015

Modeling and control of human-involved manufacturing systems poses a huge challenge on how to model all possible interactions among system components within the time and space dimensions. As the manufacturing environment are getting complicated, the importance of human in the manufacturing system is getting more and more spotlighted to incorporate the manufacturing flexibility. This paper presents a formal modeling methodology of affordance-based MPSG (Message-based Part State Graph) for a human-machine collaboration system incorporating supervisory control scheme for flexible manufacturing systems in automotive industry. Basically, we intend to extend the existing model of affordance-based MPSG to the real industrial application of humanmachine cooperative environments. The suggested extension with the real industrial example is illustrated in three steps; first, the manufacturing process and relevant data are analyzed in perspectives of MABA-MABA and the supervisory control; second, the manufacturing processes and task allocation between human and machine are mapped onto the concept of MABA-MABA; and the last, the affordance-based MPSG of humanmachine collaboration for the manufacturing process is presented with UMLs for verification.

• Information Systems Review
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• v.11 no.2
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• pp.131-145
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• 2009

Knowledge management is systematic management of vital knowledge resources and its associated processes of creating, gathering, organizing, diffusion, use and exploitation. A key challenge emerging for such organizations is how to encourage knowledge sharing within organization because knowledge is the organization's intellectual capital, of increasing importance in promoting competitive advantages. Isolated initiatives for promoting knowledge sharing and team collaboration, without taking consideration of the knowledge sharing limitations and constraints can defeat further development of KM culture. This article investigates knowledge sharing bottlenecks and proposes the use of community of practice as an effective instrument for knowledge sharing. The article demonstrates the opportunity for overcoming barriers of knowledge sharing through the application of communities of practice. The article introduces a steel company case as the best practice of communities of practice. Then, the paper empirically analyzes the case study to provide evidence for the feasibility and effectiveness of the proposed approach.